FastQCFastQC Report
Mon 15 Aug 2016
SRR2921570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647197
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135450.039.313671
GGTATCA85100.036.9068451
TCAACGC168000.030.7313584
CAACGCA172600.029.8849835
TATCAAC175250.029.7831732
ATCAACG179500.028.921263
AACGCAG190100.027.2053576
GTACATG283750.024.9718881
TACATGG295250.023.4691142
ACGCAGA225600.022.2296947
ACATGGG309900.021.5335793
CGCAGAG235400.020.9810858
CATGGGG238300.019.6922074
AGTACAT215300.019.1252162
GAGTACA231950.018.915991
GCAGAGT269250.017.2294759
AGAGTAC216150.016.41946410-11
GTAAGTA37350.016.4032692
GTGGTAT51750.016.3682461
ATGGGGG138800.016.1174165
Produced by FastQC (version 0.11.5)