##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8647197
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.115161595138865	31.0	30.0	34.0	26.0	34.0
2	30.111504225010716	31.0	30.0	34.0	26.0	34.0
3	30.29492030770202	31.0	30.0	34.0	26.0	34.0
4	33.95410408713945	35.0	35.0	37.0	30.0	37.0
5	33.48314060614093	35.0	35.0	37.0	28.0	37.0
6	33.38206473149623	35.0	33.0	37.0	28.0	37.0
7	33.1900547657235	35.0	33.0	37.0	27.0	37.0
8	33.09094102979266	35.0	33.0	37.0	26.0	37.0
9	34.41193302292061	38.0	34.0	39.0	27.0	39.0
10-11	34.21245404724791	37.0	34.0	39.0	26.5	39.0
12-13	34.04473779191107	37.0	33.0	39.0	26.0	39.0
14-15	34.925866497548284	38.0	34.0	40.0	25.0	41.0
16-17	34.782386535197475	38.0	33.0	40.0	25.0	41.0
18-19	34.65017398123346	38.0	33.5	40.0	25.0	41.0
20-21	34.51290961683884	38.0	33.0	40.0	24.0	41.0
22-23	34.34828858415045	38.0	33.0	40.0	23.5	41.0
24-25	34.209284407421265	38.0	33.0	40.0	23.0	41.0
26-27	34.12990353984071	38.0	33.0	40.0	22.5	41.0
28-29	34.145738035111265	38.0	33.0	40.0	22.5	41.0
30-31	33.99830291827514	38.0	33.0	40.0	21.5	41.0
32-33	33.83944028336582	38.0	33.0	40.0	20.5	41.0
34-35	33.65138159799066	38.0	32.0	40.0	19.0	41.0
36-37	33.49840075344646	38.0	32.0	40.0	18.0	41.0
38-39	33.33154957612276	38.0	32.0	40.0	18.0	41.0
40-41	33.33630423824044	38.0	32.0	40.0	17.5	41.0
42-43	33.142915559805104	38.0	32.0	40.0	15.5	41.0
44-45	32.954287730463406	38.0	31.5	40.0	15.0	41.0
46-47	32.7052103704819	37.0	31.0	40.0	13.0	41.0
48-49	32.4304725565984	37.0	31.0	40.0	9.0	41.0
50-51	32.169063686186405	37.0	31.0	40.0	8.5	41.0
52-53	31.857480233190017	36.5	30.0	40.0	7.5	41.0
54-55	31.549060290866507	36.0	30.0	39.0	6.5	41.0
56-57	31.218513872183088	36.0	29.5	39.0	2.0	40.0
58-59	30.75589043478482	35.0	28.5	39.0	2.0	40.0
60-61	30.411390361524088	35.0	28.0	39.0	2.0	40.0
62-63	30.082535994033673	35.0	28.0	38.0	2.0	40.0
64-65	29.634405808032362	34.0	28.0	38.0	2.0	40.0
66-67	29.219024037500244	34.0	27.5	37.0	2.0	39.5
68-69	28.795764801010087	34.0	26.0	37.0	2.0	39.0
70-71	28.331044961737312	33.5	26.0	36.0	2.0	39.0
72-73	27.858247533854033	33.0	26.0	36.0	2.0	38.5
74-75	27.321008067701012	33.0	25.5	35.0	2.0	37.5
76-77	25.803946180479063	30.5	23.0	34.0	2.0	36.0
78-79	26.683925901075227	32.0	25.0	35.0	2.0	36.0
80-81	26.74053262577457	33.0	25.0	35.0	2.0	36.0
82-83	26.422165356010737	32.5	24.5	35.0	2.0	36.0
84-85	26.00829320761398	32.0	23.5	35.0	2.0	35.0
86-87	25.572787228046266	32.0	21.0	34.5	2.0	35.0
88-89	25.137614709136383	32.0	19.5	34.0	2.0	35.0
90-91	24.721054001660885	31.0	18.0	34.0	2.0	35.0
92-93	24.315446612353114	31.0	14.5	34.0	2.0	35.0
94-95	23.942295231622456	31.0	4.5	34.0	2.0	35.0
96-97	23.41746319645545	31.0	2.0	34.0	2.0	35.0
98-99	22.67061135533283	31.0	2.0	34.0	2.0	35.0
100-101	20.102104416032155	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214831.0
3	97214.0
4	51175.0
5	36488.0
6	31241.0
7	31056.0
8	34977.0
9	40340.0
10	46151.0
11	51784.0
12	57829.0
13	62623.0
14	67974.0
15	71739.0
16	75895.0
17	78257.0
18	78265.0
19	77303.0
20	78486.0
21	81261.0
22	88185.0
23	99514.0
24	114560.0
25	135925.0
26	160848.0
27	191330.0
28	224767.0
29	263774.0
30	311646.0
31	366472.0
32	437737.0
33	530156.0
34	660744.0
35	837601.0
36	1081395.0
37	1182874.0
38	567006.0
39	27774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.745507475631484	18.511600769277756	13.63710910955277	25.105782645537992
2	16.018485527738065	20.14723383773956	38.20269157739786	25.631589057124522
3	19.749608948290046	23.59206928860391	28.66851373983283	27.989808023273216
4	14.465041606519272	15.963840894662473	34.81599436325261	34.75512313556565
5	15.094960917087691	36.699723270172555	33.467862166662925	14.737453646076823
6	34.65793799634183	34.19463545646084	16.703711421360932	14.443715125836402
7	30.313910308164278	31.075994925148727	19.98203004241549	18.62806472427151
8	27.44314778951406	34.623447014783366	19.136616139475848	18.796789056226725
9	27.162734492299336	15.964352782788401	18.66988154447966	38.2030311804326
10-11	26.315578934620298	25.060845941815057	26.9258561184081	21.69771900515655
12-13	26.81697077565009	22.01484119284696	27.132386926684067	24.03580110481889
14-15	24.08986418544554	23.687341129505178	24.228920551872175	27.99387413317711
16-17	24.427736529723536	26.762843676618704	24.91591619150428	23.893503602153483
18-19	24.486761977849195	26.276488225057314	26.036611243167346	23.200138553926145
20-21	25.01547550947113	25.350695292945897	25.951573167977372	23.682256029605604
22-23	24.863728844082072	25.019201943335617	25.784473094219056	24.332596118363252
24-25	24.612719610681953	25.242428343703494	25.734289381329845	24.410562664284704
26-27	24.44790405817859	25.462586101697642	25.80782305300261	24.28168678712116
28-29	24.54078569312736	25.642710339751908	25.639911257527014	24.176592709593717
30-31	24.89936640818749	25.41187690100535	25.480341579576205	24.208415111230956
32-33	24.659633653189296	25.463856700518683	25.43889717659485	24.43761246969717
34-35	24.752438076763532	25.451005660929958	25.65050136549884	24.14605489680767
36-37	24.7719011509377	25.387720901800968	25.60396493867716	24.23641300858417
38-39	24.780977168641275	25.460841621475794	25.53691105908833	24.221270150794602
40-41	24.834433773075475	25.279554491792318	25.56482155410257	24.32119018102964
42-43	24.6097774120281	25.287439357720633	25.484700102647306	24.61808312760396
44-45	24.488425654838583	25.26577981346299	25.682395515709334	24.56339901598909
46-47	24.582161822848818	25.154342668457915	25.63742210567479	24.62607340301848
48-49	24.46493027919258	25.291478394514737	25.650266402264094	24.59332492402859
50-51	24.431551270510965	25.30581641501845	25.70939814066488	24.553234173805706
52-53	24.533225667704812	25.273494328298206	25.591414028506026	24.60186597549096
54-55	24.249042043004675	25.134609013189934	25.792587643110902	24.82376130069449
56-57	24.349905884870164	25.229343102416347	25.64178422624641	24.778966786467084
58-59	24.45109281238975	25.144495216685144	25.76046225978832	24.643949711136784
60-61	24.584995467419706	25.15342320140892	25.742840833814316	24.51874049735706
62-63	24.411004979178774	25.135452619153515	25.704708981946727	24.748833419720988
64-65	24.26729123294009	24.99386421466574	25.774543141337038	24.964301411057136
66-67	24.297739190322208	25.108263978740847	25.598960786142396	24.995036044794546
68-69	24.13096296148845	25.112348429222532	25.638258455694523	25.118430153594502
70-71	24.137471302449452	24.96424301975391	25.652671894365714	25.245613783430926
72-73	24.146372605335667	25.135296795566735	25.480685816556843	25.23764478254076
74-75	23.926776828887196	25.152863068081437	25.594939277648898	25.325420825382473
76-77	23.935684949975624	24.920465410173684	25.689391513892467	25.454458125958222
78-79	23.87881563300184	24.958063703590803	25.76139932664679	25.40172133676057
80-81	23.92861942845669	25.096005553644556	25.58217499393351	25.393200023965246
82-83	23.800694262767376	25.058568445625372	25.684408834582772	25.45632845702448
84-85	23.836153899496267	25.05883204148355	25.518094716911882	25.5869193421083
86-87	23.576615003954416	25.12338612546455	25.66397816688352	25.636020703697515
88-89	23.52897440707416	24.93508090016818	25.694846218638013	25.841098474119654
90-91	23.63582956247314	24.831109279113736	25.690541278880414	25.84251987953271
92-93	23.676689396497224	24.80995708172424	25.62905665665826	25.884296865120277
94-95	23.5348053232181	24.848858422405392	25.615838973519793	26.00049728085671
96-97	23.30206826618815	24.76285491674233	25.748113936038802	26.186962881030716
98-99	23.143052211422372	24.768478750433758	25.66384709955207	26.424621938591798
100-101	23.461508726073475	24.605879764075752	25.6768302248756	26.25578128497517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	157.0
2	203.0
3	285.5
4	286.0
5	264.5
6	439.0
7	1016.0
8	1477.0
9	1476.5
10	1241.0
11	1018.5
12	990.0
13	1066.5
14	1224.5
15	1345.5
16	1454.0
17	1574.5
18	1738.0
19	1960.5
20	2139.5
21	2346.0
22	2637.0
23	3011.0
24	3462.0
25	3948.0
26	4774.5
27	6122.0
28	7812.0
29	10505.0
30	15094.0
31	22052.5
32	32992.0
33	47672.0
34	65254.5
35	85303.5
36	108974.5
37	134482.5
38	159283.5
39	183698.0
40	208994.5
41	233061.5
42	256192.0
43	281955.5
44	307474.0
45	331031.5
46	354269.5
47	376807.5
48	396007.0
49	409713.5
50	414661.0
51	416655.0
52	417252.0
53	412453.0
54	402781.5
55	385340.5
56	359327.5
57	325054.5
58	288789.5
59	251737.5
60	211785.5
61	173300.5
62	137931.0
63	105252.0
64	76549.5
65	54760.5
66	39657.0
67	29152.0
68	21185.5
69	15109.5
70	10693.0
71	7435.5
72	4915.0
73	3283.0
74	2285.5
75	1479.5
76	896.0
77	481.5
78	273.5
79	158.5
80	82.0
81	45.0
82	26.5
83	14.5
84	5.5
85	4.0
86	4.0
87	3.0
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2239338365946792
2	0.0
3	3.469332316587676E-5
4	0.012443338575494463
5	0.014097053646401257
6	0.10404527617446441
7	0.13499172043842647
8	0.1514941778243285
9	0.10916832356195887
10-11	0.11818858758508681
12-13	0.09406516354374718
14-15	0.11624576148779774
16-17	0.07072812149416742
18-19	0.0753770267983949
20-21	0.05214406471831277
22-23	0.0785861591912385
24-25	0.07098832141791149
26-27	0.049640363229842
28-29	0.017416048229270134
30-31	0.02999237787690046
32-33	0.03702934025904579
34-35	0.04223912095445495
36-37	0.03501712751542494
38-39	0.03609262053356712
40-41	0.02069456726844549
42-43	0.029460413588357014
44-45	0.01687830172019904
46-47	0.029518235793633475
48-49	3.4115101113112146E-4
50-51	0.01577967981994628
52-53	0.0077944332712669775
54-55	0.015305537736679295
56-57	0.010084192600214845
58-59	0.007291380085361765
60-61	0.029633880204186395
62-63	0.012634151852906787
64-65	0.013842635943184824
66-67	0.05660793896565557
68-69	0.07581069333796836
70-71	0.03158248852200314
72-73	0.05346819321914373
74-75	0.04630402198539018
76-77	0.20011108802077712
78-79	0.07415697826706157
80-81	0.015531044337257495
82-83	0.010442690272928904
84-85	0.023539419768047378
86-87	0.16698474661789248
88-89	0.3773361471931309
90-91	0.27689319440739
92-93	0.2769394521716112
94-95	0.0019890838615102676
96-97	0.0056203183528720346
98-99	0.004862847463750392
100-101	0.005071007402745653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8647197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.18894259781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31642198659117	37.5299011654843
2	13.42835403254259	13.74766487509786
3	5.130667627533782	7.879003520228064
4	2.5891994390492625	5.301535258390982
5	1.431978067162113	3.6650721540642155
6	0.8981670287683841	2.758573228732222
7	0.6110649564755214	2.1895838286391114
8	0.4245554594741013	1.7386036035685295
9	0.3199077498262161	1.4738165498203863
>10	1.6909868956230394	15.732536536756445
>50	0.1133954020954626	3.9605493726538024
>100	0.044551946540962256	3.680516817303518
>500	6.132406003442014E-4	0.1942807320805642
>1k	1.3616771708551724E-4	0.14836235717984964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1564441055292252E-5
2	0.0	0.0	0.0	0.0	1.1564441055292252E-5
3	0.0	0.0	0.0	0.0	1.1564441055292252E-5
4	0.0	0.0	0.0	0.0	2.3128882110584504E-5
5	0.0	0.0	0.0	0.0	2.3128882110584504E-5
6	0.0	0.0	0.0	0.0	3.469332316587676E-5
7	0.0	0.0	0.0	0.0	3.469332316587676E-5
8	0.0	0.0	0.0	0.0	3.469332316587676E-5
9	0.0	0.0	0.0	1.1564441055292252E-5	3.469332316587676E-5
10-11	0.0	0.0	0.0	5.203998474881513E-5	3.469332316587676E-5
12-13	0.0	0.0	0.0	7.516886685939963E-5	5.7822205276461265E-5
14-15	0.0	0.0	0.0	8.67333079146919E-5	1.2142663108056866E-4
16-17	0.0	0.0	0.0	1.098621900252764E-4	1.6768439530173766E-4
18-19	0.0	0.0	0.0	1.2720885160821477E-4	1.8503105688467603E-4
20-21	0.0	0.0	0.0	1.8503105688467606E-4	1.8503105688467603E-4
22-23	0.0	0.0	0.0	3.353687906034753E-4	2.0815993899526053E-4
24-25	0.0	0.0	0.0	5.608753911816743E-4	2.370710416334912E-4
26-27	0.0	0.0	0.0	0.0011159685618357023	2.833288058546602E-4
28-29	0.0	0.0	0.0	0.00410537657462875	3.0645768796524466E-4
30-31	0.0	0.0	0.0	0.01562934208622748	3.353687906034753E-4
32-33	0.0	5.782220527646126E-6	0.0	0.040677921411990496	3.816265548246443E-4
34-35	0.0	1.1564441055292252E-5	0.0	0.07441139597027799	4.0475543693522885E-4
36-37	0.0	1.734666158293838E-5	0.0	0.12942344207030326	4.0475543693522885E-4
38-39	0.0	2.3128882110584504E-5	0.0	0.21997879775376922	4.278843190458133E-4
40-41	0.0	2.8911102638230632E-5	0.0	0.32263634100159855	4.278843190458133E-4
42-43	0.0	3.469332316587676E-5	0.0	0.43252744212951316	4.3366653957345946E-4
44-45	0.0	3.469332316587676E-5	0.0	0.5652004921363536	4.452309806287517E-4
46-47	0.0	3.469332316587676E-5	0.0	0.7201697844977974	4.741420832669823E-4
48-49	0.0	3.469332316587676E-5	0.0	0.8853620427521196	4.857065243222746E-4
50-51	0.0	3.469332316587676E-5	0.0	1.0628819951713833	4.857065243222746E-4
52-53	1.1564441055292252E-5	3.469332316587676E-5	0.0	1.2470804123000783	5.261820680157975E-4
54-55	1.1564441055292252E-5	3.469332316587676E-5	0.0	1.4577035772401161	5.782220527646126E-4
56-57	1.1564441055292252E-5	3.469332316587676E-5	0.0	1.7011524081156009	5.897864938199049E-4
58-59	1.1564441055292252E-5	3.469332316587676E-5	0.0	1.9664580325855883	5.95568714347551E-4
60-61	1.1564441055292252E-5	3.469332316587676E-5	0.0	2.2511745713668834	6.013509348751972E-4
62-63	1.1564441055292252E-5	3.469332316587676E-5	0.0	2.5488779774532717	6.244798169857816E-4
64-65	1.1564441055292252E-5	3.469332316587676E-5	0.0	2.8744805975855527	6.244798169857816E-4
66-67	1.1564441055292252E-5	3.469332316587676E-5	0.0	3.2442015603437735	6.360442580410739E-4
68-69	1.1564441055292252E-5	4.047554369352288E-5	0.0	3.650194392471919	6.360442580410739E-4
70-71	1.1564441055292252E-5	4.625776422116901E-5	0.0	4.087544206521489	6.4182647856872E-4
72-73	1.1564441055292252E-5	4.625776422116901E-5	0.0	4.544079428281789	7.632531096492887E-4
74-75	1.1564441055292252E-5	4.625776422116901E-5	0.0	5.03261924066261	7.80599771232227E-4
76-77	1.1564441055292252E-5	4.625776422116901E-5	0.0	5.56582092439897	7.863819917598732E-4
78-79	1.1564441055292252E-5	4.625776422116901E-5	0.0	6.146222874302506	7.863819917598732E-4
80-81	1.1564441055292252E-5	4.625776422116901E-5	0.0	6.757137601930429	8.326397559810421E-4
82-83	1.1564441055292252E-5	4.625776422116901E-5	0.0	7.3920138514249185	9.13590843368088E-4
84-85	1.1564441055292252E-5	4.625776422116901E-5	0.0	8.053332195392333	9.13590843368088E-4
86-87	1.1564441055292252E-5	5.7822205276461265E-5	0.0	8.749667666875173	9.193730638957341E-4
88-89	1.1564441055292252E-5	5.7822205276461265E-5	0.0	9.497239394453487	9.251552844233802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13545	0.0	39.31367	1
GGTATCA	8510	0.0	36.906845	1
TCAACGC	16800	0.0	30.731358	4
CAACGCA	17260	0.0	29.884983	5
TATCAAC	17525	0.0	29.783173	2
ATCAACG	17950	0.0	28.92126	3
AACGCAG	19010	0.0	27.205357	6
GTACATG	28375	0.0	24.971888	1
TACATGG	29525	0.0	23.469114	2
ACGCAGA	22560	0.0	22.229694	7
ACATGGG	30990	0.0	21.533579	3
CGCAGAG	23540	0.0	20.981085	8
CATGGGG	23830	0.0	19.692207	4
AGTACAT	21530	0.0	19.125216	2
GAGTACA	23195	0.0	18.91599	1
GCAGAGT	26925	0.0	17.229475	9
AGAGTAC	21615	0.0	16.419464	10-11
GTAAGTA	3735	0.0	16.403269	2
GTGGTAT	5175	0.0	16.368246	1
ATGGGGG	13880	0.0	16.117416	5
>>END_MODULE