FastQCFastQC Report
Mon 15 Aug 2016
SRR2921569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051636
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG231650.028.628641
GTATCAA145950.027.8120841
TACATGG240850.026.8781432
ACATGGG259100.024.3107173
TCAACGC168950.023.7667684
TATCAAC172150.023.1658152
CAACGCA172950.023.1360595
GGTATCA105300.022.9893341
ATCAACG176450.022.626943
CATGGGG194800.022.1223284
GAGTACA178150.022.0618061
AACGCAG182500.022.0258946
AGTACAT169500.021.7661082
ATGGGGG113450.019.1535135
ACGCAGA213650.018.3801177
GTATAGG16600.017.7435671
CGCAGAG219100.017.7117848
TGGGGGG93700.016.8955296
GTGTAGC31850.016.7616831
GTATAAG18000.016.619191
Produced by FastQC (version 0.11.5)