FastQCFastQC Report
Mon 15 Aug 2016
SRR2921569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051636
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG223900.028.037031
GTATCAA144600.026.6927831
TACATGG238650.025.9661752
ACATGGG245900.024.6218873
GGTATCA106350.022.7335641
GAGTACA178100.022.6874181
TCAACGC166800.022.604464
AGTACAT167500.022.594482
CAACGCA168350.022.4243625
CATGGGG191550.022.2371924
TATCAAC173650.021.9856932
ATCAACG176800.021.4065043
AACGCAG179200.021.2222946
GTATAGG13750.020.7677691
GTAAGTA27800.019.1307542
ATGGGGG111250.018.9517365
ACGCAGA214000.017.3496537
AGAGTAC160250.017.2757385
GTACACT51700.016.808554
TACACTG74600.016.8047165
Produced by FastQC (version 0.11.5)