##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6051636
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11891346406162	31.0	30.0	34.0	26.0	34.0
2	30.102964388472802	31.0	30.0	34.0	26.0	34.0
3	30.29354558007124	31.0	30.0	34.0	26.0	34.0
4	33.940929361911394	35.0	35.0	37.0	30.0	37.0
5	33.45618655847774	35.0	35.0	37.0	28.0	37.0
6	33.35162442023942	35.0	33.0	37.0	28.0	37.0
7	33.16034226116706	35.0	33.0	37.0	27.0	37.0
8	33.05796449092444	35.0	33.0	37.0	26.0	37.0
9	34.38841034060872	38.0	34.0	39.0	27.0	39.0
10-11	34.18702215400926	37.0	34.0	39.0	26.0	39.0
12-13	34.00114836715228	37.0	33.0	39.0	25.0	39.0
14-15	34.878735436169656	38.0	34.0	40.0	25.0	41.0
16-17	34.72861289079515	38.0	33.0	40.0	25.0	41.0
18-19	34.59777116469001	38.0	33.5	40.0	24.5	41.0
20-21	34.45335377078199	38.0	33.0	40.0	23.5	41.0
22-23	34.28778118842574	38.0	33.0	40.0	23.0	41.0
24-25	34.136066181112014	38.0	33.0	40.0	22.0	41.0
26-27	34.051130223298294	38.0	33.0	40.0	21.5	41.0
28-29	34.069032902838174	38.0	33.0	40.0	21.5	41.0
30-31	33.917598150318355	38.0	33.0	40.0	20.0	41.0
32-33	33.74442704419103	38.0	33.0	40.0	19.0	41.0
34-35	33.54743403271446	38.0	32.0	40.0	18.0	41.0
36-37	33.381312921001864	38.0	32.0	40.0	17.5	41.0
38-39	33.20678259564852	38.0	32.0	40.0	16.0	41.0
40-41	33.22425621765751	38.0	32.0	40.0	15.5	41.0
42-43	33.02781454469502	38.0	32.0	40.0	15.0	41.0
44-45	32.82743121033717	37.5	31.0	40.0	13.5	41.0
46-47	32.562086186280865	37.0	31.0	40.0	9.0	41.0
48-49	32.27452262495629	37.0	31.0	40.0	8.5	41.0
50-51	32.00405411032653	37.0	30.5	40.0	7.5	41.0
52-53	31.68866146278461	36.0	30.0	40.0	6.5	41.0
54-55	31.371351399852866	36.0	30.0	39.0	2.0	41.0
56-57	31.026400712138006	35.5	29.0	39.0	2.0	40.0
58-59	30.552249837895076	35.0	28.5	39.0	2.0	40.0
60-61	30.189396883751765	35.0	28.0	38.5	2.0	40.0
62-63	29.84450551883821	34.5	28.0	38.0	2.0	40.0
64-65	29.39739212669103	34.0	27.5	38.0	2.0	40.0
66-67	28.976203624937124	34.0	26.5	37.0	2.0	39.5
68-69	28.543224757734933	34.0	26.0	36.5	2.0	39.0
70-71	28.077824575040534	33.5	26.0	36.0	2.0	39.0
72-73	27.606022569764605	33.0	26.0	35.5	2.0	38.5
74-75	27.08043981825741	33.0	24.5	35.0	2.0	37.5
76-77	25.586466370416197	30.5	22.0	34.0	2.0	36.0
78-79	26.467125501269408	32.0	24.0	35.0	2.0	36.0
80-81	26.51855052088394	33.0	24.0	35.0	2.0	36.0
82-83	26.210691208129504	32.0	23.5	35.0	2.0	36.0
84-85	25.801695855467845	32.0	22.5	35.0	2.0	35.0
86-87	25.3738840207838	32.0	20.0	34.5	2.0	35.0
88-89	24.944679339603375	32.0	19.0	34.0	2.0	35.0
90-91	24.533052054023077	31.0	17.0	34.0	2.0	35.0
92-93	24.130408702704525	31.0	9.5	34.0	2.0	35.0
94-95	23.75937168064966	31.0	4.5	34.0	2.0	35.0
96-97	23.244548746818218	31.0	2.0	34.0	2.0	35.0
98-99	22.515046096625774	31.0	2.0	34.0	2.0	35.0
100-101	19.944586885265405	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	154272.0
3	72189.0
4	38172.0
5	27465.0
6	23248.0
7	23002.0
8	26057.0
9	29896.0
10	34003.0
11	37341.0
12	41678.0
13	45417.0
14	49154.0
15	52111.0
16	54647.0
17	56915.0
18	56483.0
19	55443.0
20	55473.0
21	57882.0
22	62338.0
23	70061.0
24	80974.0
25	94426.0
26	111393.0
27	131866.0
28	154793.0
29	182757.0
30	213440.0
31	254463.0
32	302254.0
33	368635.0
34	462979.0
35	586681.0
36	759007.0
37	820414.0
38	384229.0
39	20078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.993424340574634	17.85893838947033	12.669657098642679	25.47798017131236
2	16.451022500361887	19.5100795883956	37.52770325247586	26.511194658766655
3	21.072362676621896	22.383844479535096	27.84019440719093	28.70359843665209
4	14.707933619158647	14.627514261792937	33.93620857601547	36.72834354303295
5	15.230914110356444	36.64787639274339	33.025371827943054	15.09583766895711
6	36.04608462025298	32.90029268951125	16.01250158387141	15.041121106364367
7	31.44164546166669	30.63466407738973	18.715087866305414	19.208602594638172
8	28.449348737326265	34.260716243304	18.417037158646476	18.872897860723263
9	27.580027963550773	15.682532970985003	18.193942377616455	38.543496687847764
10-11	27.324263544999777	24.66944540813634	25.813269102648157	22.193021944215722
12-13	27.32790977660991	21.374027430829557	26.49992863015718	24.79813416240336
14-15	24.351316260537594	23.139976719516497	23.714386915282994	28.794320104662912
16-17	24.862908087337654	26.4533586536761	24.3985447086693	24.285188550316946
18-19	24.83923080828368	25.966719491558077	25.638996246582824	23.555053453575418
20-21	25.167398480468194	24.94272078660918	25.790253093275922	24.099627639646705
22-23	25.090378381954093	24.59289210822253	25.571004498247007	24.745725011576372
24-25	24.87109782625142	24.805176046209574	25.539030232986736	24.78469589455227
26-27	24.64451998832123	25.02424535205111	25.74966330951618	24.58157135011148
28-29	24.605377687349996	25.452745603442228	25.481858897458153	24.460017811749623
30-31	25.077515112926267	25.043348626431204	25.339053203186623	24.540083057455895
32-33	24.7922776532363	25.09873454800811	25.35960766376148	24.74938013499411
34-35	24.92974325695209	25.118301370121177	25.591308472790868	24.360646900135862
36-37	24.904855096912005	25.109220546571372	25.640524430539795	24.345399925976828
38-39	24.925562028997582	25.24970525671264	25.45750506006283	24.367227654226948
40-41	24.99005633161483	25.054449486867043	25.446378360197556	24.509115821320567
42-43	24.680261343225098	24.9866626620905	25.38585300819679	24.947222986487613
44-45	24.506632633751323	24.979551658650124	25.62756864469765	24.8862470629009
46-47	24.643025920322444	24.94149815308219	25.51846678134797	24.897009145247395
48-49	24.502574932476705	25.07568640767795	25.564597219089453	24.857141440755896
50-51	24.454501642312664	25.07159603967465	25.699895911390147	24.774006406622533
52-53	24.566194266867257	25.07799170220345	25.510018840299846	24.845795190629445
54-55	24.114053690320137	24.85982190380419	25.855548530548134	25.17057587532754
56-57	24.44045444362997	24.972684084234906	25.573630099490835	25.01323137264429
58-59	24.546450977192304	24.973040192856303	25.664059030223658	24.81644979972774
60-61	24.77527609786641	24.958266918820048	25.665146550514343	24.6013104327992
62-63	24.480626308345073	24.89880093141072	25.709930461508407	24.910642298735798
64-65	24.203359169112357	24.813445600915713	25.78006446108949	25.20313076888244
66-67	24.35070241488728	24.92282583634175	25.470586518683625	25.255885230087344
68-69	24.124246579816546	24.90802268725481	25.605582596019598	25.362148136909042
70-71	24.25450106134125	24.7403633578204	25.553103717541646	25.452031863296703
72-73	24.14227531113259	24.92108638569033	25.4586678165051	25.477970486671982
74-75	23.802636992135742	24.994968029733386	25.600970172133476	25.601424805997397
76-77	23.88580539348642	24.59206689034775	25.693471795001738	25.828655921164096
78-79	23.868533360058027	24.523734868547916	25.827936301295786	25.77979547009828
80-81	23.94112971340227	24.7151942222487	25.592751598854903	25.75092446549413
82-83	23.827771170025052	24.729923600716777	25.685712033420078	25.75659319583809
84-85	23.816188320643196	24.627181994292556	25.575998230132523	25.980631454931725
86-87	23.441236542034012	24.791784130898222	25.670435895899352	26.096543431168417
88-89	23.38744946802974	24.568003024972025	25.75452823101258	26.290019275985653
90-91	23.589090714162207	24.297949548128074	25.819447920785976	26.293511816923743
92-93	23.70237058503672	24.336627168101067	25.697553406578326	26.263448840283882
94-95	23.492831161710473	24.264284074197427	25.759978491295595	26.4829062727965
96-97	23.084308670816256	24.154676988667607	25.97174664573116	26.78926769478498
98-99	22.97916526201817	24.108973621692797	25.93609080705484	26.975770309234193
100-101	23.312502086319594	23.87730391653003	25.898489025380556	26.911704971769822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	128.5
2	130.5
3	197.0
4	196.0
5	196.5
6	367.0
7	686.5
8	1042.0
9	1172.0
10	1005.0
11	862.0
12	894.0
13	988.0
14	1069.5
15	1155.0
16	1259.5
17	1363.0
18	1482.5
19	1626.0
20	1785.0
21	1950.5
22	2202.5
23	2482.5
24	2785.0
25	3240.5
26	3994.0
27	5060.5
28	6425.5
29	8792.0
30	12860.0
31	18531.5
32	26557.5
33	36507.0
34	47373.0
35	59201.5
36	74253.0
37	89194.0
38	101325.0
39	114266.5
40	127943.5
41	140635.5
42	153498.0
43	170744.0
44	189388.0
45	208140.0
46	226457.0
47	245555.5
48	265718.5
49	278209.0
50	285691.0
51	291694.5
52	294896.0
53	296882.0
54	294504.0
55	285005.0
56	268594.5
57	247703.5
58	224239.5
59	197279.0
60	168092.0
61	139157.5
62	111287.0
63	85146.5
64	62241.0
65	44469.5
66	32034.0
67	23484.0
68	17285.5
69	12323.5
70	8660.0
71	6188.0
72	4459.0
73	3260.0
74	2294.5
75	1502.0
76	1018.5
77	609.0
78	305.5
79	152.5
80	84.0
81	50.5
82	29.5
83	20.0
84	9.5
85	2.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24500812672804512
2	0.0
3	1.3219565750484662E-4
4	0.014111886438642376
5	0.01632616370184856
6	0.10446761834320503
7	0.13632677180187308
8	0.15235549527433573
9	0.109458004414013
10-11	0.1185299314102831
12-13	0.09361104997061952
14-15	0.11712535254929411
16-17	0.07146001510996365
18-19	0.07622732100873218
20-21	0.05277911625881001
22-23	0.07991227496167978
24-25	0.07134434390964692
26-27	0.05046569225247519
28-29	0.018763521137094168
30-31	0.03053719688361957
32-33	0.03858460753422711
34-35	0.04250916611640224
36-37	0.036006792212882596
38-39	0.03807234936139583
40-41	0.021539629944695948
42-43	0.031239486314114065
44-45	0.016904519703432264
46-47	0.03019018328266935
48-49	3.470136009502224E-4
50-51	0.016887995246244156
52-53	0.008452259851716132
54-55	0.01701192867515495
56-57	0.011178795287753594
58-59	0.008237441908270755
60-61	0.03104945505645085
62-63	0.013459170379712197
64-65	0.014111886438642376
66-67	0.05726550638538075
68-69	0.07648345009514783
70-71	0.032710163003855486
72-73	0.053704485861343944
74-75	0.04647503584154764
76-77	0.2243195063285366
78-79	0.07921824775977933
80-81	0.016152656901373446
82-83	0.010955715115714163
84-85	0.02421659200916909
86-87	0.17829889305966187
88-89	0.382549446133244
90-91	0.2775365207028314
92-93	0.2772060315590693
94-95	0.0021894905774240223
96-97	0.0058579200731835165
98-99	0.004957337156431749
100-101	0.005122581728312807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6051636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.33385244908441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0124118756835	34.80637290101979
2	13.424600007483722	12.977252718993912
3	5.46495777459196	7.924273881528135
4	2.734256105309546	5.286285246081592
5	1.5966592742486327	3.8586346886497807
6	1.0265924822684565	2.977150173798172
7	0.7176639530388833	2.428122452994562
8	0.49481353757176744	1.9132995611842631
9	0.35742709869615713	1.5548245784715946
>10	1.9899127868421973	17.310860994838915
>50	0.12760561571339396	4.2144106837328765
>100	0.05179268959847192	4.12355686960931
>500	9.629113707640788E-4	0.29723087514179847
>1k	3.438875826190982E-4	0.32772437395537574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.6524457188105828E-5	0.0
7	0.0	0.0	0.0	1.6524457188105828E-5	0.0
8	0.0	0.0	0.0	1.6524457188105828E-5	0.0
9	0.0	0.0	0.0	6.609782875242331E-5	3.3048914376211655E-5
10-11	0.0	0.0	0.0	6.609782875242331E-5	3.3048914376211655E-5
12-13	0.0	8.262228594052914E-6	0.0	6.609782875242331E-5	3.3048914376211655E-5
14-15	0.0	1.6524457188105828E-5	0.0	8.262228594052914E-5	5.78356001583704E-5
16-17	0.0	1.6524457188105828E-5	0.0	1.4045788609889952E-4	8.262228594052914E-5
18-19	0.0	1.6524457188105828E-5	0.0	1.5698234328700539E-4	9.914674312863497E-5
20-21	0.0	1.6524457188105828E-5	0.0	2.0655571485132286E-4	1.156712003167408E-4
22-23	0.0	1.6524457188105828E-5	0.0	3.057024579799578E-4	1.3219565750484662E-4
24-25	0.0	3.3048914376211655E-5	0.0	4.874714870491219E-4	1.5698234328700539E-4
26-27	0.0	4.957337156431748E-5	0.0	0.0010906141744149846	1.817690290691641E-4
28-29	1.6524457188105828E-5	4.957337156431748E-5	0.0	0.004775568127362584	1.817690290691641E-4
30-31	1.6524457188105828E-5	4.957337156431748E-5	0.0	0.017962084963471035	1.90031257663217E-4
32-33	1.6524457188105828E-5	4.957337156431748E-5	0.0	0.04502914583758838	2.313424006334816E-4
34-35	1.6524457188105828E-5	6.609782875242331E-5	0.0	0.0818043253097179	2.6439131500969324E-4
36-37	1.6524457188105828E-5	9.914674312863497E-5	0.0	0.14495253845406433	2.6439131500969324E-4
38-39	1.6524457188105828E-5	1.652445718810583E-4	0.0	0.2474454841632907	2.6439131500969324E-4
40-41	1.6524457188105828E-5	2.0655571485132286E-4	0.0	0.36387680951068435	2.6439131500969324E-4
42-43	1.6524457188105828E-5	2.1481794344537576E-4	0.0	0.47726763473546663	2.6439131500969324E-4
44-45	1.6524457188105828E-5	2.1481794344537576E-4	0.0	0.6117932407038361	2.6439131500969324E-4
46-47	1.6524457188105828E-5	2.313424006334816E-4	0.0	0.7761454918967368	2.809157721977991E-4
48-49	1.6524457188105828E-5	2.4786685782158743E-4	0.0	0.9541304202698246	2.974402293859049E-4
50-51	1.6524457188105828E-5	2.6439131500969324E-4	0.0	1.1463181195960894	2.974402293859049E-4
52-53	1.6524457188105828E-5	2.809157721977991E-4	0.0	1.341133207615263	3.1396468657401077E-4
54-55	2.478668578215874E-5	2.974402293859049E-4	0.0	1.560222723243764	3.552758295442753E-4
56-57	3.3048914376211655E-5	3.139646865740107E-4	0.0	1.814691101712	3.7180028673238115E-4
58-59	3.3048914376211655E-5	3.139646865740107E-4	0.0	2.0937065613331667	3.8832474392048696E-4
60-61	3.3048914376211655E-5	3.470136009502224E-4	0.0	2.4023668971497956	3.9658697251453986E-4
62-63	3.3048914376211655E-5	3.635380581383282E-4	0.0	2.7193142482462593	3.9658697251453986E-4
64-65	3.3048914376211655E-5	3.635380581383282E-4	0.0	3.04544919753931	3.9658697251453986E-4
66-67	3.3048914376211655E-5	3.8006251532643405E-4	0.0	3.4153161227806828	3.9658697251453986E-4
68-69	3.3048914376211655E-5	3.8006251532643405E-4	0.0	3.8319803107787713	3.9658697251453986E-4
70-71	3.3048914376211655E-5	3.8006251532643405E-4	0.0	4.2893359746025705	4.131114297026457E-4
72-73	3.3048914376211655E-5	3.8006251532643405E-4	0.0	4.755796614337015	4.2963588689075153E-4
74-75	3.3048914376211655E-5	3.8006251532643405E-4	0.0	5.234551450219412	4.2963588689075153E-4
76-77	3.3048914376211655E-5	3.8006251532643405E-4	0.0	5.75167607569259	4.2963588689075153E-4
78-79	3.3048914376211655E-5	3.8006251532643405E-4	0.0	6.335369476948052	4.2963588689075153E-4
80-81	3.3048914376211655E-5	3.8006251532643405E-4	0.0	6.964282055298765	4.2963588689075153E-4
82-83	3.3048914376211655E-5	3.965869725145399E-4	0.0	7.595921169085516	4.2963588689075153E-4
84-85	3.3048914376211655E-5	4.131114297026457E-4	0.0	8.235368088893647	4.2963588689075153E-4
86-87	4.131114297026457E-5	4.131114297026457E-4	0.0	8.91367226977961	4.2963588689075153E-4
88-89	4.957337156431748E-5	4.131114297026457E-4	0.0	9.641508511086919	4.544225726729103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	22390	0.0	28.03703	1
GTATCAA	14460	0.0	26.692783	1
TACATGG	23865	0.0	25.966175	2
ACATGGG	24590	0.0	24.621887	3
GGTATCA	10635	0.0	22.733564	1
GAGTACA	17810	0.0	22.687418	1
TCAACGC	16680	0.0	22.60446	4
AGTACAT	16750	0.0	22.59448	2
CAACGCA	16835	0.0	22.424362	5
CATGGGG	19155	0.0	22.237192	4
TATCAAC	17365	0.0	21.985693	2
ATCAACG	17680	0.0	21.406504	3
AACGCAG	17920	0.0	21.222294	6
GTATAGG	1375	0.0	20.767769	1
GTAAGTA	2780	0.0	19.130754	2
ATGGGGG	11125	0.0	18.951736	5
ACGCAGA	21400	0.0	17.349653	7
AGAGTAC	16025	0.0	17.275738	5
GTACACT	5170	0.0	16.80855	4
TACACTG	7460	0.0	16.804716	5
>>END_MODULE