FastQCFastQC Report
Mon 15 Aug 2016
SRR2921568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097491
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110350.042.746211
GGTATCA60700.039.8788951
TCAACGC144050.032.404134
TATCAAC146350.031.6443542
CAACGCA149750.031.0780665
ATCAACG154550.030.172893
AACGCAG156100.029.8677886
GTACATG231750.027.0658861
TACATGG247750.025.0350532
ACGCAGA184800.024.6264957
ACATGGG259300.023.7068313
CGCAGAG191600.023.2464688
CATGGGG189100.021.3558674
GCAGAGT199600.020.819139
GAGTACA177700.019.9152151
AGTACAT169050.019.4864352
GTATAGG13550.019.0193141
ATGGGGG97450.018.6691575
ATATGTA13400.017.8584861
AGAGTAC173800.017.7481210-11
Produced by FastQC (version 0.11.5)