##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921568_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5097491
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.335604123675747	31.0	28.0	33.0	16.0	34.0
2	28.306016038086188	31.0	28.0	33.0	16.0	34.0
3	28.394122912625054	31.0	28.0	34.0	16.0	34.0
4	31.833850417783964	35.0	32.0	37.0	19.0	37.0
5	31.781192943744283	35.0	32.0	37.0	19.0	37.0
6	31.883520932160547	35.0	32.0	37.0	19.0	37.0
7	31.694375527097545	35.0	32.0	37.0	18.0	37.0
8	31.645942091903645	35.0	32.0	37.0	18.0	37.0
9	32.8953008450628	37.0	32.0	39.0	17.0	39.0
10-11	32.681756377794486	37.0	32.0	39.0	17.0	39.0
12-13	32.499282293975604	37.0	32.0	39.0	17.0	39.0
14-15	33.234158432060006	38.0	32.0	40.0	16.0	41.0
16-17	33.100255105894256	38.0	32.0	40.0	15.0	41.0
18-19	32.934515431219005	38.0	32.0	40.0	11.0	41.0
20-21	32.808543261773295	38.0	32.0	40.0	10.0	41.0
22-23	32.59473699904522	37.5	31.0	40.0	10.0	41.0
24-25	32.694522756391336	38.0	31.5	40.0	10.0	41.0
26-27	32.46475432717782	38.0	31.0	40.0	9.0	41.0
28-29	32.27737930287665	37.0	31.0	40.0	8.0	41.0
30-31	32.03339397754699	37.0	30.0	40.0	8.0	41.0
32-33	31.79886958113315	37.0	30.0	40.0	2.0	41.0
34-35	31.46544898264656	37.0	30.0	40.0	2.0	41.0
36-37	31.170904862804072	37.0	30.0	40.0	2.0	41.0
38-39	30.957188938636676	36.0	29.5	40.0	2.0	41.0
40-41	30.736114982841556	36.0	29.0	39.0	2.0	41.0
42-43	30.486371530621632	36.0	28.0	39.0	2.0	40.0
44-45	30.223633057910256	35.5	28.0	39.0	2.0	40.0
46-47	29.92446372146611	35.0	27.0	39.0	2.0	40.0
48-49	29.631603174973726	35.0	27.0	39.0	2.0	40.0
50-51	28.67020961881051	34.0	25.0	38.0	2.0	39.5
52-53	28.85875237445245	34.5	26.0	38.0	2.0	39.5
54-55	29.44270926618605	35.0	27.0	39.0	2.0	40.0
56-57	29.42181045537893	35.0	27.0	39.0	2.0	40.0
58-59	29.1747501859248	35.0	26.0	39.0	2.0	40.0
60-61	28.825621859852227	34.0	26.0	38.0	2.0	40.0
62-63	28.401241905086245	34.0	26.0	38.0	2.0	40.0
64-65	27.93288590406535	34.0	25.0	37.5	2.0	40.0
66-67	27.58499642274994	34.0	24.0	37.0	2.0	39.5
68-69	27.121354603666784	33.0	22.5	36.0	2.0	39.0
70-71	26.697289803944727	33.0	22.0	36.0	2.0	39.0
72-73	26.220166548602045	32.5	20.5	35.0	2.0	38.0
74-75	25.776218339571372	32.0	20.0	35.0	2.0	37.0
76-77	25.352675855631723	32.0	18.0	35.0	2.0	37.0
78-79	24.960957655442648	32.0	16.0	35.0	2.0	36.0
80-81	24.519081740409156	31.0	10.0	35.0	2.0	36.0
82-83	23.979228800992487	31.0	6.0	34.0	2.0	35.0
84-85	23.567129593755045	31.0	2.0	34.0	2.0	35.0
86-87	23.07391606968997	30.5	2.0	34.0	2.0	35.0
88-89	22.5613626389924	30.0	2.0	34.0	2.0	35.0
90-91	21.969383369190844	29.5	2.0	34.0	2.0	35.0
92-93	21.42317514636122	29.0	2.0	34.0	2.0	35.0
94-95	20.739058195492646	29.0	2.0	34.0	2.0	35.0
96-97	20.093642244782778	29.0	2.0	34.0	2.0	35.0
98	19.612399511838277	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	297386.0
3	49544.0
4	35801.0
5	29438.0
6	27224.0
7	28233.0
8	32329.0
9	37104.0
10	38614.0
11	41091.0
12	41293.0
13	41009.0
14	40045.0
15	42012.0
16	45460.0
17	48623.0
18	50798.0
19	50117.0
20	51793.0
21	55667.0
22	59860.0
23	66975.0
24	76574.0
25	88249.0
26	100979.0
27	116617.0
28	135093.0
29	155233.0
30	180326.0
31	210577.0
32	249071.0
33	301828.0
34	371837.0
35	461826.0
36	568280.0
37	575842.0
38	274823.0
39	19920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62532697883072	17.57386381190263	12.959174898573348	24.8416343106933
2	16.454297046603173	19.36162195168912	38.55687416648133	25.627206835226374
3	20.186621375112107	22.995863445570073	28.016958792609408	28.800556386708408
4	14.0633076039494	14.931097232396825	34.84668552217762	36.15890964147615
5	15.20756319656084	36.59907994152297	32.84826421370124	15.345092648214944
6	34.573651474678165	33.23084743700208	16.856120007615296	15.339381080704454
7	30.847201634856532	30.921777343232538	19.518719778439493	18.712301243471437
8	27.851535077478847	33.32114254859865	19.340398756382996	19.486923617539503
9	27.781664649112308	15.794095661506043	18.763392865901633	37.660846823480014
10-11	26.68301428214242	24.65874867664846	26.30245599724329	22.355781043965823
12-13	27.023975244222093	22.007312512758347	26.518840368452707	24.449871874566853
14-15	24.544425486254998	23.515729253249308	23.938300788804778	28.001544471690913
16-17	24.54856743529134	26.504715541644035	24.698534048885705	24.248182974178924
18-19	24.524042134642627	25.862852886464438	26.178779288723973	23.434325690168958
20-21	25.22795069276018	25.00053757903271	25.871886914187364	23.899624814019745
22-23	25.262511505221756	24.570646823714377	25.64055635099042	24.526285320073452
24-25	24.736900332671734	24.98771218265307	25.620405934316352	24.654981550358848
26-27	24.496076633638076	25.137622406703773	25.865924316094713	24.500376643563442
28-29	24.570468348157164	25.239786836942596	25.776838259373264	24.41290655552698
30-31	24.958265387273155	25.049131738440384	25.51201412001839	24.480588754268073
32-33	24.715079541286034	25.19143740991765	25.35529886565529	24.73818418314103
34-35	24.93598155788624	25.143844058839594	25.61016186054751	24.310012522726655
36-37	24.953562542999467	24.959088935315403	25.52490876034647	24.56243976133866
38-39	24.894079177950232	25.115763730414013	25.448128069877068	24.542029021758687
40-41	24.87217558813213	24.986208690819232	25.51571421453152	24.62590150651712
42-43	24.783995271346278	24.835532792630737	25.393368622342493	24.98710331368049
44-45	24.476424253006996	24.95618372067981	25.65936044276343	24.908031583549764
46-47	24.835329946960076	24.8420333949834	25.443680159496786	24.87895649855974
48-49	24.56365648600701	25.0174907496868	25.31979429224769	25.099058472058505
50-51	24.499338994296878	25.060123417260204	25.50630595798876	24.93423163045416
52-53	24.60940342824684	25.039148042479702	25.553673613308437	24.797774915965025
54-55	24.25973737995758	24.787076739416904	25.827635571834236	25.125550308791283
56-57	24.455122809401033	24.84635657403661	25.608337000534576	25.090183616027783
58-59	24.437006855644068	24.738636690235364	25.795694483869962	25.02866197025061
60-61	24.72368485943343	24.718782696467223	25.676171186287018	24.88136125781233
62-63	24.450211469620843	24.675796783630048	25.863286853919114	25.01070489282999
64-65	24.259972403689815	24.475889163845586	25.945808644251265	25.318329788213333
66-67	24.345368898246292	24.790312725109835	25.64730941582597	25.2170089608179
68-69	24.303248153638247	24.61602398574746	25.669775853666387	25.410952006947905
70-71	24.19596573663056	24.53668440769489	25.875281791847787	25.392068063826763
72-73	24.337214898037715	24.646871608271667	25.637572363020546	25.378341130670073
74-75	24.131687197456227	24.79387722135968	25.7925335989229	25.281901982261196
76-77	24.085854091213797	24.478869786459732	25.94978875000566	25.485487372320808
78-79	24.054425797895103	24.433226124146863	25.970161148295983	25.542186929662048
80-81	24.238123143960717	24.726400368632877	25.643073618263696	25.392402869142707
82-83	24.265017681055447	24.552621382988917	25.77159197188977	25.410768964065873
84-85	24.194406186053833	24.55954863843853	25.711081378876123	25.53496379663151
86-87	24.01273588975188	24.673661516165453	25.92055179149358	25.393050802589084
88-89	24.053589874940297	24.31276399322776	25.949726184054462	25.683919947777483
90-91	24.242055462689347	24.240264928261055	25.98362566103432	25.534053948015277
92-93	24.34882749354914	24.325297389722557	25.88437334523217	25.441501771496135
94-95	24.2735803761526	24.193985415133472	25.965391441918307	25.567042766795616
96-97	24.230385452189932	24.08674282874041	25.99663876261128	25.686232956458376
98	24.133652992098124	24.05392195252107	25.925830456233918	25.886594599146882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	257.0
1	221.0
2	270.5
3	380.0
4	401.5
5	478.0
6	730.0
7	1324.5
8	1909.0
9	2055.0
10	1953.5
11	1936.5
12	2061.0
13	2291.0
14	2527.5
15	2712.5
16	2838.0
17	2880.5
18	2850.0
19	2794.0
20	2780.0
21	2708.5
22	2750.0
23	2958.5
24	3128.0
25	3564.5
26	4349.5
27	5204.5
28	6157.5
29	7920.0
30	10957.0
31	16319.5
32	25552.0
33	36457.5
34	48248.5
35	62008.0
36	76363.5
37	89993.0
38	102837.0
39	113096.0
40	122800.0
41	134002.0
42	147946.0
43	166008.5
44	180916.0
45	193666.5
46	207794.0
47	222551.5
48	234810.0
49	239476.5
50	239899.0
51	240644.0
52	243282.5
53	246805.0
54	246039.0
55	238894.5
56	224233.5
57	204228.0
58	183964.0
59	162883.5
60	140046.5
61	115652.5
62	92394.0
63	70801.0
64	51614.0
65	37085.5
66	26614.5
67	19208.5
68	13758.0
69	9771.5
70	6884.0
71	4852.5
72	3519.5
73	2608.5
74	1957.5
75	1388.5
76	884.5
77	502.0
78	294.5
79	179.0
80	117.5
81	72.5
82	41.5
83	29.0
84	24.5
85	27.0
86	18.5
87	8.5
88	8.5
89	12.5
90	13.0
91	8.5
92	8.5
93	10.5
94	10.0
95	7.0
96	5.5
97	6.0
98	4.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30877935831568903
2	0.14954415809660085
3	0.147248911278117
4	0.14128519304889406
5	0.13624349704589964
6	0.04867100304836242
7	0.039195753361800936
8	0.014615033160431277
9	0.011005414232217379
10-11	0.0021873506005209227
12-13	0.006620904284087995
14-15	0.011819540240483014
16-17	0.00699363667341443
18-19	0.051662670910061444
20-21	0.10233465836428156
22-23	0.10275643448904569
24-25	0.07908792776681704
26-27	0.0878765651572509
28-29	0.10829837659350453
30-31	0.08428656372321207
32-33	0.02182446226977154
34-35	0.3281712513077512
36-37	0.4285833952428754
38-39	0.39689133340304084
40-41	0.39270299839666223
42-43	0.36619976376613517
44-45	0.34836746156099146
46-47	0.35341896631107345
48-49	0.34650379961435934
50-51	0.347847597965352
52-53	0.3980291480651952
54-55	0.3834533498931141
56-57	0.3665724961554616
58-59	0.3450521050454037
60-61	0.35508645331595484
62-63	0.35386035993001264
64-65	0.3710354760802913
66-67	0.3944783816195065
68-69	0.35261464904989537
70-71	0.32590543073052997
72-73	0.3086714620977261
74-75	0.2935169478474803
76-77	0.2966949819038425
78-79	0.30458121456222287
80-81	0.29284014429844013
82-83	0.30403192472532076
84-85	0.296047604596065
86-87	0.29125112727025904
88-89	0.31697947088087064
90-91	0.2983722776558115
92-93	0.2872099234701935
94-95	0.3042869521495967
96-97	0.3027764050981159
98	0.30220749776703876
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5097491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.93061958022135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96189468425726	31.012680694379867
2	10.997261682265865	9.222439920464701
3	4.778119563311362	6.010485411540663
4	2.655470764133752	4.453821376691682
5	1.655387411277059	3.4705709900072894
6	1.138254374370649	2.863662669435512
7	0.7787009454979028	2.2855989178701868
8	0.5749743949637361	1.9287226098873886
9	0.4437950454356814	1.6747741099575542
>10	2.6739723698097513	21.06158080506448
>50	0.22434294038107255	6.487647007805464
>100	0.11408703402436829	8.325194724909366
>500	0.0033128526369928667	0.8723940824743475
>1k	4.259376348750327E-4	0.3304266795115416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.923498835015108E-5
9	0.0	0.0	0.0	3.923498835015108E-5	5.885248252522663E-5
10-11	0.0	0.0	0.0	3.923498835015108E-5	5.885248252522663E-5
12-13	0.0	0.0	0.0	5.885248252522663E-5	5.885248252522663E-5
14-15	0.0	0.0	0.0	6.86612296127644E-5	1.4713120631306656E-4
16-17	0.0	0.0	0.0	1.1770496505045326E-4	1.7655744757567987E-4
18-19	0.0	0.0	0.0	1.1770496505045326E-4	1.7655744757567987E-4
20-21	0.0	0.0	0.0	1.373224592255288E-4	1.7655744757567987E-4
22-23	0.0	0.0	0.0	2.746449184510576E-4	2.354099301009065E-4
24-25	0.0	0.0	0.0	7.650822728279462E-4	2.746449184510576E-4
26-27	0.0	0.0	0.0	0.0020304106471203186	3.727323893264353E-4
28-29	0.0	0.0	0.0	0.007356560315653328	3.727323893264353E-4
30-31	9.80874708753777E-6	0.0	0.0	0.027121185697041937	4.0215863058904865E-4
32-33	1.961749417507554E-5	0.0	0.0	0.07087800645454793	4.806286072893508E-4
34-35	1.961749417507554E-5	9.80874708753777E-6	0.0	0.1310644785836797	5.492898369021152E-4
36-37	1.961749417507554E-5	1.961749417507554E-5	0.0	0.2265820577221225	5.492898369021152E-4
38-39	1.961749417507554E-5	1.961749417507554E-5	0.0	0.37482165245608084	5.492898369021152E-4
40-41	1.961749417507554E-5	1.961749417507554E-5	0.0	0.5359597496101514	5.689073310771907E-4
42-43	1.961749417507554E-5	3.923498835015108E-5	0.0	0.7044838333211378	5.885248252522663E-4
44-45	1.961749417507554E-5	3.923498835015108E-5	0.0	0.9030226831199898	6.081423194273418E-4
46-47	1.961749417507554E-5	3.923498835015108E-5	0.0	1.1383639519912836	6.375685606899551E-4
48-49	1.961749417507554E-5	3.923498835015108E-5	0.0	1.3860053897103497	6.86612296127644E-4
50-51	1.961749417507554E-5	3.923498835015108E-5	0.0	1.652587518055451	6.86612296127644E-4
52-53	1.961749417507554E-5	3.923498835015108E-5	1.961749417507554E-5	1.9237895662787832	7.454647786528706E-4
54-55	3.923498835015108E-5	5.885248252522663E-5	1.961749417507554E-5	2.230999525060466	8.239347553531727E-4
56-57	3.923498835015108E-5	5.885248252522663E-5	1.961749417507554E-5	2.5790923417030065	8.53360996615786E-4
58-59	3.923498835015108E-5	5.885248252522663E-5	1.961749417507554E-5	2.960201401042199	8.631697437033239E-4
60-61	3.923498835015108E-5	7.846997670030216E-5	1.961749417507554E-5	3.364625852208469	8.827872378783994E-4
62-63	3.923498835015108E-5	7.846997670030216E-5	1.961749417507554E-5	3.7772504159399203	8.827872378783994E-4
64-65	5.885248252522663E-5	7.846997670030216E-5	1.961749417507554E-5	4.23047338386669	8.827872378783994E-4
66-67	7.846997670030216E-5	7.846997670030216E-5	1.961749417507554E-5	4.737507138315692	9.02404732053475E-4
68-69	7.846997670030216E-5	9.808747087537771E-5	1.961749417507554E-5	5.297920094415076	9.220222262285505E-4
70-71	7.846997670030216E-5	9.808747087537771E-5	1.961749417507554E-5	5.903051128486544	9.612572145787016E-4
72-73	7.846997670030216E-5	1.1770496505045326E-4	1.961749417507554E-5	6.525180721260714	9.80874708753777E-4
74-75	7.846997670030216E-5	1.1770496505045326E-4	1.961749417507554E-5	7.163112205592908	9.80874708753777E-4
76-77	7.846997670030216E-5	1.1770496505045326E-4	1.961749417507554E-5	7.858915297741575	9.906834558413148E-4
78-79	7.846997670030216E-5	1.1770496505045326E-4	1.961749417507554E-5	8.623340384514655	0.0010004922029288527
80-81	7.846997670030216E-5	1.1770496505045326E-4	2.942624126261331E-5	9.437682185216218	0.001029918444191466
82-83	7.846997670030216E-5	1.1770496505045326E-4	3.923498835015108E-5	10.268129948635515	0.0011378146621543815
84-85	7.846997670030216E-5	1.1770496505045326E-4	3.923498835015108E-5	11.11201569556474	0.0011378146621543815
86	7.846997670030216E-5	1.1770496505045326E-4	3.923498835015108E-5	11.762551419904419	0.0011378146621543815
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11035	0.0	42.74621	1
GGTATCA	6070	0.0	39.878895	1
TCAACGC	14405	0.0	32.40413	4
TATCAAC	14635	0.0	31.644354	2
CAACGCA	14975	0.0	31.078066	5
ATCAACG	15455	0.0	30.17289	3
AACGCAG	15610	0.0	29.867788	6
GTACATG	23175	0.0	27.065886	1
TACATGG	24775	0.0	25.035053	2
ACGCAGA	18480	0.0	24.626495	7
ACATGGG	25930	0.0	23.706831	3
CGCAGAG	19160	0.0	23.246468	8
CATGGGG	18910	0.0	21.355867	4
GCAGAGT	19960	0.0	20.81913	9
GAGTACA	17770	0.0	19.915215	1
AGTACAT	16905	0.0	19.486435	2
GTATAGG	1355	0.0	19.019314	1
ATGGGGG	9745	0.0	18.669157	5
ATATGTA	1340	0.0	17.858486	1
AGAGTAC	17380	0.0	17.74812	10-11
>>END_MODULE