FastQCFastQC Report
Mon 15 Aug 2016
SRR2921568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097491
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.044.93281
GTATCAA108600.043.396351
TCAACGC143700.032.226164
TATCAAC148200.031.2143542
CAACGCA149050.030.972925
ATCAACG155350.029.9011783
AACGCAG155100.029.5769546
GTACATG226400.025.2530841
ACGCAGA181600.024.4241317
TACATGG245100.023.1756252
CGCAGAG191550.022.7337478
ACATGGG252350.022.2283523
GCAGAGT202200.020.1702149
GTATAGG13850.019.9354521
CATGGGG182800.019.6169474
GAGTACA180800.019.2208371
AGTACAT173700.018.6751672
AGAGTAC179450.017.69962510-11
GTGGTAT36350.017.5488321
CATATAG8700.017.4699383
Produced by FastQC (version 0.11.5)