##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5097491
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.992216366836157	31.0	30.0	34.0	26.0	34.0
2	29.987789875450492	31.0	30.0	34.0	26.0	34.0
3	30.16498057573814	31.0	30.0	34.0	26.0	34.0
4	33.81309510894673	35.0	35.0	37.0	30.0	37.0
5	33.31623361375233	35.0	33.0	37.0	27.0	37.0
6	33.206672655233724	35.0	33.0	37.0	27.0	37.0
7	33.011953920075584	35.0	33.0	37.0	26.0	37.0
8	32.90697403879673	35.0	33.0	37.0	26.0	37.0
9	34.21358958750491	37.0	34.0	39.0	26.0	39.0
10-11	34.00730751658021	37.0	33.0	39.0	25.0	39.0
12-13	33.82891926636064	37.0	33.0	39.0	25.0	39.0
14-15	34.68421523451439	38.0	33.0	40.0	25.0	41.0
16-17	34.53657907390126	38.0	33.0	40.0	24.0	41.0
18-19	34.41379003906039	38.0	33.0	40.0	23.5	41.0
20-21	34.2841414531188	38.0	33.0	40.0	22.5	41.0
22-23	34.109291316061174	38.0	33.0	40.0	21.5	41.0
24-25	33.952051999699464	38.0	33.0	40.0	20.0	41.0
26-27	33.85750470182292	38.0	32.5	40.0	19.5	41.0
28-29	33.86136689598864	38.0	33.0	40.0	19.0	41.0
30-31	33.69940211763003	38.0	32.0	40.0	18.5	41.0
32-33	33.53113904467904	38.0	32.0	40.0	18.0	41.0
34-35	33.32132906168937	38.0	32.0	40.0	16.5	41.0
36-37	33.14991384977433	38.0	32.0	40.0	16.0	41.0
38-39	32.96846389723886	38.0	31.0	40.0	15.0	41.0
40-41	32.97896533804572	38.0	32.0	40.0	15.0	41.0
42-43	32.77710455987073	37.5	31.0	40.0	12.5	41.0
44-45	32.56803533346111	37.0	31.0	40.0	9.0	41.0
46-47	32.29066848769325	37.0	31.0	40.0	8.5	41.0
48-49	31.990903662213434	37.0	30.0	40.0	7.5	41.0
50-51	31.71863040072067	36.5	30.0	40.0	4.0	41.0
52-53	31.39534949644835	36.0	30.0	39.0	2.0	41.0
54-55	31.070813661073654	36.0	29.0	39.0	2.0	40.5
56-57	30.723219619220515	35.0	29.0	39.0	2.0	40.0
58-59	30.24586732963334	35.0	28.0	39.0	2.0	40.0
60-61	29.88582186805234	35.0	28.0	38.0	2.0	40.0
62-63	29.542548579291264	34.0	27.5	38.0	2.0	40.0
64-65	29.087965432405866	34.0	26.5	37.5	2.0	40.0
66-67	28.671493485716795	34.0	26.0	37.0	2.0	39.0
68-69	28.23733430819201	34.0	26.0	36.0	2.0	39.0
70-71	27.785916542079228	33.0	26.0	36.0	2.0	39.0
72-73	27.32848258094031	33.0	25.0	35.0	2.0	38.0
74-75	26.816128463983553	32.5	24.0	35.0	2.0	37.0
76-77	25.3226380389882	30.5	21.5	34.0	2.0	35.5
78-79	26.21581215150748	32.0	23.5	35.0	2.0	36.0
80-81	26.28738314594376	32.0	24.0	35.0	2.0	36.0
82-83	25.991442064341065	32.0	23.0	35.0	2.0	35.5
84-85	25.59956221599999	32.0	20.5	35.0	2.0	35.0
86-87	25.190270370266468	32.0	20.0	34.0	2.0	35.0
88-89	24.771698763175845	31.5	18.5	34.0	2.0	35.0
90-91	24.368942093276868	31.0	15.0	34.0	2.0	35.0
92-93	23.98291178934892	31.0	8.5	34.0	2.0	35.0
94-95	23.628080265369768	31.0	2.0	34.0	2.0	35.0
96-97	23.126336368225076	31.0	2.0	34.0	2.0	35.0
98-99	22.39759167794509	31.0	2.0	34.0	2.0	35.0
100-101	19.808902654266582	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141154.0
3	63108.0
4	33090.0
5	23651.0
6	20554.0
7	20616.0
8	23066.0
9	26537.0
10	29954.0
11	32962.0
12	36337.0
13	39424.0
14	42446.0
15	45367.0
16	47835.0
17	49549.0
18	48011.0
19	46449.0
20	47005.0
21	48574.0
22	52558.0
23	58986.0
24	68220.0
25	80702.0
26	94743.0
27	112794.0
28	132389.0
29	155637.0
30	182614.0
31	216113.0
32	257460.0
33	313931.0
34	393413.0
35	499384.0
36	639883.0
37	671066.0
38	289027.0
39	12882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.627108228198466	18.886676558242797	13.595922232153129	24.890292981405608
2	16.326188707346418	20.261928858726776	37.50882542019201	25.9030570137348
3	19.89450880404403	23.722846178911293	28.317051127349615	28.065593889695062
4	14.530599559840518	15.810464196183126	34.35929955114877	35.299636692827576
5	15.144001641853771	36.88902850052524	32.91781430963463	15.049155547986354
6	34.60450876212789	33.6851211242546	16.632008303082134	15.078361810535371
7	30.67271964727179	31.08365312178022	19.561898831278405	18.681728399669588
8	27.86033455781733	33.58274245775294	19.280603045760678	19.27631993866905
9	27.533000845457945	15.71078857365058	18.731518495497273	38.02469208539419
10-11	26.5761227155329	24.81919688890198	26.380225672450873	22.22445472311424
12-13	26.948300296844863	22.05242672025943	26.62673390633997	24.372539076555736
14-15	24.44192349246821	23.644249070666287	23.902215650987294	28.011611785878216
16-17	24.519608817978018	26.667672455975534	24.708503510168697	24.104215215877755
18-19	24.550965976415437	25.935666490008057	26.138959682670222	23.374407850906284
20-21	25.155553802154763	25.06043835150753	25.84491510170739	23.939092744630326
22-23	25.104459699048085	24.69912710810299	25.66383261396476	24.532580578884165
24-25	24.715166181812865	25.081876440659716	25.617841363531202	24.58511601399622
26-27	24.498114894755403	25.214325347437782	25.868111132199566	24.419448625607245
28-29	24.49401962998404	25.301971455037297	25.83684859153893	24.367160323439727
30-31	24.900890795609172	25.111756641719786	25.561010491976482	24.426342070694563
32-33	24.678630345710577	25.224608451243775	25.376996797587847	24.719764405457802
34-35	24.86014340446707	25.20555919234331	25.723103910195032	24.211193492994585
36-37	24.86705026941221	25.064716037351324	25.539810543077007	24.528423150159455
38-39	24.83369485417133	25.13623019689721	25.50026954611652	24.529805402814937
40-41	24.767501215803353	24.98872493843478	25.632588101717197	24.611185744044672
42-43	24.718494224473446	24.84436014136073	25.488191583074936	24.948954051090887
44-45	24.372923258038405	24.969690726706396	25.746096681627545	24.911289333627646
46-47	24.73009528126665	24.86339682474271	25.510722699233607	24.89578519475703
48-49	24.412458648612223	25.05938729224747	25.401439262724594	25.126714796415712
50-51	24.37758311310029	25.047322430886197	25.568680972895226	25.00641348311829
52-53	24.552634488243832	25.052046849079883	25.53249741200397	24.862821250672322
54-55	24.230675353632307	24.843859078403618	25.691950717702788	25.23351485026129
56-57	24.403617543784613	24.93249379676704	25.444771852282322	25.219116807166024
58-59	24.344775582221974	24.876186694387062	25.635100188058303	25.143937535332668
60-61	24.640966363427108	24.989035455657028	25.43007995924624	24.93991822166963
62-63	24.323723263095708	24.89400787664359	25.564943069638623	25.217325790622073
64-65	24.09906549262427	24.683398703428526	25.658000260754427	25.559535543192773
66-67	24.185738203343423	24.92342580141969	25.393642268231353	25.497193727005534
68-69	24.12922974894178	24.752622394526284	25.401802406343123	25.71634545018881
70-71	23.950676252391286	24.6129417486788	25.601002307469727	25.835379691460187
72-73	24.10432061641663	24.66006089422494	25.352726814500297	25.882891674858126
74-75	23.76088442170027	24.80006382570323	25.493245898138735	25.94580585445777
76-77	23.69670678523809	24.465270042155517	25.814431380274883	26.023591792331512
78-79	23.591396587039828	24.423609694536157	25.795111659700552	26.189882058723455
80-81	23.7069094065498	24.677399354563264	25.490827012908735	26.1248642259782
82-83	23.630384883152082	24.523304300176466	25.602627904693133	26.24368291197832
84-85	23.50652594680975	24.453787003519437	25.552266441037684	26.487420608633133
86-87	23.288515872970642	24.580853309614458	25.753440163914938	26.377190653499966
88-89	23.25168936465553	24.291641481231462	25.730684490159984	26.725984663953025
90-91	23.23385076700318	24.185920063987997	25.822366625366005	26.75786254364282
92-93	23.24731518912028	24.202735372854853	25.760256664605368	26.7896927734195
94-95	22.993947107916625	24.107829131647165	25.87142498976929	27.026798770666915
96-97	22.832512262117085	24.026618127628037	25.964161129152597	27.176708481102285
98-99	22.654184936682203	23.948340124068533	25.901201693810805	27.496273245438456
100-101	22.905151068624612	23.702129362619434	26.024223243382266	27.368496325373687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	182.0
2	196.5
3	288.0
4	256.5
5	256.0
6	472.0
7	979.5
8	1373.5
9	1393.5
10	1204.5
11	991.0
12	977.0
13	1034.0
14	1097.5
15	1229.5
16	1332.0
17	1365.0
18	1474.0
19	1606.5
20	1731.5
21	1900.5
22	2092.5
23	2293.0
24	2516.5
25	2880.0
26	3484.0
27	4400.5
28	5376.5
29	6683.5
30	9225.5
31	13143.5
32	19985.0
33	29232.5
34	39246.5
35	50081.5
36	64278.5
37	79229.5
38	90166.0
39	101311.5
40	112105.5
41	121468.5
42	132136.0
43	148618.0
44	165662.0
45	179704.0
46	193327.5
47	206644.5
48	221142.5
49	232461.5
50	237665.0
51	238640.5
52	240850.0
53	245141.5
54	246080.5
55	239719.0
56	224674.5
57	203545.0
58	184256.5
59	165337.0
60	142964.5
61	118314.0
62	93810.5
63	71881.5
64	52689.0
65	37782.5
66	27040.5
67	19314.0
68	13888.5
69	9975.5
70	7047.0
71	4913.0
72	3505.0
73	2655.5
74	1983.0
75	1413.0
76	938.5
77	533.5
78	259.5
79	132.5
80	70.0
81	45.5
82	29.5
83	15.0
84	7.5
85	4.0
86	4.0
87	4.5
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24239375802723337
2	0.0
3	7.846997670030216E-5
4	0.013771480910903031
5	0.015949022764336414
6	0.10605217351045838
7	0.1350860648895702
8	0.15160399498498378
9	0.10938714752022122
10-11	0.11850928231163134
12-13	0.09344793350297234
14-15	0.11652791539994872
16-17	0.07155481000358804
18-19	0.07577257125122927
20-21	0.05159400968044867
22-23	0.07885251783671614
24-25	0.07233950977059106
26-27	0.05002461014644263
28-29	0.01765574475756799
30-31	0.03132913819759564
32-33	0.03839143610062283
34-35	0.04281518103710237
36-37	0.03468372970153356
38-39	0.03703782900254263
40-41	0.020922057537718065
42-43	0.031231050726720264
44-45	0.01698874995561542
46-47	0.02993629611116528
48-49	3.9234988350151084E-4
50-51	0.015821509052198424
52-53	0.007856806417117754
54-55	0.015949022764336414
56-57	0.010828856784641698
58-59	0.00787642391129283
60-61	0.029818591146114824
62-63	0.012751371213799101
64-65	0.014546371930818515
66-67	0.057332126726658265
68-69	0.076733828465808
70-71	0.03235905664178711
72-73	0.05404619645233312
74-75	0.046395373724053654
76-77	0.2244731770983019
78-79	0.07894079656050398
80-81	0.015438967915784451
82-83	0.00985779082297546
84-85	0.023913725399417086
86-87	0.17651821258732975
88-89	0.3790982661862473
90-91	0.27538057448262293
92-93	0.27710691397002957
94-95	0.0018244269582820252
96-97	0.005649838322421757
98-99	0.0044629799248296865
100-101	0.004668963613667979
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5097491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.513428260344085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9560691290774	27.327614780387382
2	11.866024668180813	9.1400257958691
3	5.364523943974351	6.1981862420146285
4	3.1132764135034487	4.7961179122434565
5	1.8668448414741035	3.5949297437653156
6	1.2755835647915301	2.9476257667603543
7	0.9095148530610134	2.4519974531557915
8	0.6745334061651562	2.078287515803783
9	0.5080266864152215	1.7609264407433662
>10	3.059157784408863	22.122762804598374
>50	0.267770436031928	7.114913754389181
>100	0.13373671991113717	8.989270740146269
>500	0.004320359549424	1.0447623742168566
>1k	6.171934556586112E-4	0.43257867590595833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.961749417507554E-5
2	0.0	0.0	0.0	0.0	1.961749417507554E-5
3	0.0	0.0	0.0	0.0	1.961749417507554E-5
4	0.0	0.0	0.0	0.0	1.961749417507554E-5
5	0.0	1.961749417507554E-5	0.0	0.0	1.961749417507554E-5
6	0.0	1.961749417507554E-5	0.0	0.0	1.1770496505045326E-4
7	0.0	1.961749417507554E-5	0.0	0.0	1.1770496505045326E-4
8	0.0	1.961749417507554E-5	0.0	0.0	1.373224592255288E-4
9	0.0	1.961749417507554E-5	0.0	1.961749417507554E-5	1.373224592255288E-4
10-11	0.0	1.961749417507554E-5	0.0	1.961749417507554E-5	1.5693995340060432E-4
12-13	9.80874708753777E-6	3.923498835015108E-5	0.0	3.923498835015108E-5	1.5693995340060432E-4
14-15	1.961749417507554E-5	3.923498835015108E-5	0.0	5.885248252522662E-5	2.4521867718844427E-4
16-17	1.961749417507554E-5	3.923498835015108E-5	0.0	1.1770496505045326E-4	2.9426241262613313E-4
18-19	1.961749417507554E-5	3.923498835015108E-5	0.0	1.1770496505045326E-4	3.5311489515135974E-4
20-21	1.961749417507554E-5	3.923498835015108E-5	0.0	1.5693995340060432E-4	3.82541136413973E-4
22-23	1.961749417507554E-5	3.923498835015108E-5	0.0	3.1387990680120865E-4	4.315848718516619E-4
24-25	1.961749417507554E-5	3.923498835015108E-5	0.0	8.337435024407106E-4	4.5120236602673745E-4
26-27	1.961749417507554E-5	3.923498835015108E-5	0.0	0.0021088806238206206	4.904373543768885E-4
28-29	1.961749417507554E-5	3.923498835015108E-5	0.0	0.007415412798178555	4.904373543768885E-4
30-31	1.961749417507554E-5	3.923498835015108E-5	0.0	0.02694462824946626	5.100548485519641E-4
32-33	1.961749417507554E-5	3.923498835015108E-5	0.0	0.07062297903027195	5.296723427270396E-4
34-35	1.961749417507554E-5	4.9043735437688855E-5	0.0	0.12984819394482502	6.081423194273418E-4
36-37	1.961749417507554E-5	6.86612296127644E-5	0.0	0.22482629199345325	6.179510665148796E-4
38-39	1.961749417507554E-5	7.846997670030216E-5	0.0	0.37387020398858967	6.375685606899551E-4
40-41	1.961749417507554E-5	7.846997670030216E-5	0.0	0.535479121002862	6.473773077774929E-4
42-43	1.961749417507554E-5	7.846997670030216E-5	2.942624126261331E-5	0.7034539148769463	6.86612296127644E-4
44-45	1.961749417507554E-5	7.846997670030216E-5	3.923498835015108E-5	0.9021398958821114	7.356560315653328E-4
46-47	2.942624126261331E-5	7.846997670030216E-5	3.923498835015108E-5	1.136676847492227	7.74891019915484E-4
48-49	3.923498835015108E-5	7.846997670030216E-5	3.923498835015108E-5	1.3815424097855198	9.024047320534749E-4
50-51	3.923498835015108E-5	7.846997670030216E-5	3.923498835015108E-5	1.6432397820810276	9.024047320534749E-4
52-53	3.923498835015108E-5	7.846997670030216E-5	3.923498835015108E-5	1.9101848340683683	9.710659616662394E-4
54-55	3.923498835015108E-5	9.808747087537771E-5	3.923498835015108E-5	2.2118724682397675	0.0010593446854540792
56-57	3.923498835015108E-5	9.808747087537771E-5	3.923498835015108E-5	2.55278528201423	0.0010789621796291548
58-59	3.923498835015108E-5	9.808747087537771E-5	3.923498835015108E-5	2.92617485739553	0.0010887709267166927
60-61	3.923498835015108E-5	1.0789621796291548E-4	3.923498835015108E-5	3.3230661907985715	0.0011181971679793058
62-63	3.923498835015108E-5	1.1770496505045326E-4	3.923498835015108E-5	3.7274808332177534	0.0011378146621543815
64-65	3.923498835015108E-5	1.2751371213799104E-4	3.923498835015108E-5	4.171748415053601	0.0011378146621543815
66-67	3.923498835015108E-5	1.373224592255288E-4	3.923498835015108E-5	4.668836099955842	0.0011378146621543815
68-69	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	5.217763013215718	0.0011476234092419192
70-71	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	5.812800846534108	0.0012162846388546835
72-73	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	6.425013796002778	0.0013928420864303636
74-75	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	7.057746644378577	0.001412459580605439
76-77	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	7.748458996788813	0.001412459580605439
78-79	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	8.508587852337552	0.001412459580605439
80-81	3.923498835015108E-5	1.373224592255288E-4	5.885248252522663E-5	9.315180742839958	0.0014320770747805146
82-83	5.885248252522663E-5	1.373224592255288E-4	5.885248252522663E-5	10.139537274317895	0.0014811208102182036
84-85	5.885248252522663E-5	1.373224592255288E-4	5.885248252522663E-5	10.978449986473738	0.0014909295573057413
86-87	5.885248252522663E-5	1.373224592255288E-4	5.885248252522663E-5	11.853566784129683	0.001500738304393279
88-89	5.885248252522663E-5	1.373224592255288E-4	5.885248252522663E-5	12.787320271874929	0.0015105470514808167
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5880	0.0	44.9328	1
GTATCAA	10860	0.0	43.39635	1
TCAACGC	14370	0.0	32.22616	4
TATCAAC	14820	0.0	31.214354	2
CAACGCA	14905	0.0	30.97292	5
ATCAACG	15535	0.0	29.901178	3
AACGCAG	15510	0.0	29.576954	6
GTACATG	22640	0.0	25.253084	1
ACGCAGA	18160	0.0	24.424131	7
TACATGG	24510	0.0	23.175625	2
CGCAGAG	19155	0.0	22.733747	8
ACATGGG	25235	0.0	22.228352	3
GCAGAGT	20220	0.0	20.170214	9
GTATAGG	1385	0.0	19.935452	1
CATGGGG	18280	0.0	19.616947	4
GAGTACA	18080	0.0	19.220837	1
AGTACAT	17370	0.0	18.675167	2
AGAGTAC	17945	0.0	17.699625	10-11
GTGGTAT	3635	0.0	17.548832	1
CATATAG	870	0.0	17.469938	3
>>END_MODULE