FastQCFastQC Report
Mon 15 Aug 2016
SRR2921567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8718444
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131450.039.5602341
GGTATCA77900.034.6179351
TCAACGC164500.031.3639374
CAACGCA170200.030.0960545
TATCAAC172300.029.7046262
ATCAACG174150.029.4152873
AACGCAG183500.027.8336096
GTACATG278250.026.5448841
TACATGG290350.024.7479442
ACATGGG304150.022.9149783
ACGCAGA219900.022.6370727
CGCAGAG229900.021.2113198
GAGTACA217400.020.4271971
CATGGGG224250.020.323384
AGTACAT202400.019.634952
GCAGAGT252150.018.1185689
AGAGTAC201900.016.91465410-11
GTATTAG20150.016.2152751
GTATAGG21750.016.0803431
GTATACG5350.015.4831641
Produced by FastQC (version 0.11.5)