##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921567_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8718444
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.060435669484143	31.0	28.0	31.0	16.0	34.0
2	28.03170370767995	31.0	28.0	33.0	16.0	34.0
3	28.130150058886656	31.0	28.0	34.0	16.0	34.0
4	31.53432149131198	35.0	32.0	37.0	17.0	37.0
5	31.461205462809648	35.0	32.0	37.0	17.0	37.0
6	31.552192226044006	35.0	32.0	37.0	17.0	37.0
7	31.355540277599992	35.0	32.0	37.0	17.0	37.0
8	31.30266134645127	35.0	32.0	37.0	17.0	37.0
9	32.5337959388166	37.0	32.0	39.0	17.0	39.0
10-11	32.30947018757017	37.0	32.0	39.0	15.0	39.0
12-13	32.11039062704308	37.0	32.0	39.0	11.0	39.0
14-15	32.816292792613	38.0	32.0	40.0	10.0	41.0
16-17	32.66814640318846	38.0	31.5	40.0	10.0	41.0
18-19	32.48466308896404	38.0	31.0	40.0	10.0	41.0
20-21	32.3448745555973	37.0	31.0	40.0	10.0	41.0
22-23	32.11662172745504	37.0	30.5	40.0	8.0	41.0
24-25	32.2292144102778	37.0	31.0	40.0	8.0	41.0
26-27	31.984240593849087	37.0	30.0	40.0	4.5	41.0
28-29	31.791209704392205	37.0	30.0	40.0	2.0	41.0
30-31	31.545899761471198	37.0	30.0	40.0	2.0	41.0
32-33	31.302156611890837	37.0	30.0	40.0	2.0	41.0
34-35	30.954895908031297	36.5	29.5	40.0	2.0	41.0
36-37	30.65322739929281	36.0	28.5	39.5	2.0	41.0
38-39	30.433266130974747	36.0	28.0	39.0	2.0	41.0
40-41	30.207992446817343	36.0	27.5	39.0	2.0	40.0
42-43	29.956089068186937	35.0	27.0	39.0	2.0	40.0
44-45	29.687097147151487	35.0	26.5	39.0	2.0	40.0
46-47	29.398203567058527	35.0	26.0	39.0	2.0	40.0
48-49	29.107436946317485	35.0	25.5	39.0	2.0	40.0
50-51	28.192333976108582	34.0	23.5	38.0	2.0	39.5
52-53	28.39908543313463	34.0	25.0	38.0	2.0	39.5
54-55	28.96391454713708	35.0	26.0	39.0	2.0	40.0
56-57	28.93639036965771	35.0	26.0	39.0	2.0	40.0
58-59	28.687369615495612	34.5	26.0	39.0	2.0	40.0
60-61	28.338266438369047	34.0	25.0	38.0	2.0	40.0
62-63	27.913261070438715	34.0	23.5	38.0	2.0	40.0
64-65	27.440764372633467	34.0	22.5	37.5	2.0	40.0
66-67	27.08183467141614	33.0	22.0	37.0	2.0	39.5
68-69	26.620545535418934	33.0	20.5	36.0	2.0	39.0
70-71	26.19160947756274	32.5	19.5	36.0	2.0	39.0
72-73	25.70789478030713	32.0	18.0	35.0	2.0	38.5
74-75	25.250700813126745	32.0	15.0	35.0	2.0	37.5
76-77	24.807568013283102	31.5	9.5	35.0	2.0	37.0
78-79	24.39520538297889	31.0	7.0	35.0	2.0	36.0
80-81	23.941226152281303	31.0	2.0	34.5	2.0	36.0
82-83	23.391784933183033	31.0	2.0	34.0	2.0	35.0
84-85	22.96287026675861	30.0	2.0	34.0	2.0	35.0
86-87	22.450899610068035	30.0	2.0	34.0	2.0	35.0
88-89	21.923448725483585	29.5	2.0	34.0	2.0	35.0
90-91	21.32052365077989	29.0	2.0	34.0	2.0	35.0
92-93	20.763045160352007	29.0	2.0	34.0	2.0	35.0
94-95	20.076691895939227	28.5	2.0	34.0	2.0	35.0
96-97	19.423507222160282	27.0	2.0	33.5	2.0	35.0
98	18.935810220264074	26.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	559350.0
3	93724.0
4	67640.0
5	55077.0
6	50475.0
7	52502.0
8	60181.0
9	67970.0
10	72133.0
11	75592.0
12	76658.0
13	74494.0
14	72913.0
15	75964.0
16	80766.0
17	85078.0
18	89343.0
19	90615.0
20	94437.0
21	100622.0
22	109005.0
23	121117.0
24	136602.0
25	155088.0
26	176274.0
27	203339.0
28	233217.0
29	267317.0
30	308682.0
31	359287.0
32	421780.0
33	505596.0
34	617044.0
35	760963.0
36	925197.0
37	931269.0
38	458969.0
39	32163.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70136295734499	17.261169521961673	12.740206505341655	25.29726101535168
2	16.3233155974497	18.89722235592069	38.8626182566146	25.916843790015008
3	20.50879922624473	22.317027153227322	27.776964124795605	29.39720949573234
4	14.032649020900376	14.69083038339392	34.90580352122779	36.370717074477916
5	15.41671146051847	35.95175219765528	33.181137607631584	15.450398734194662
6	35.1147158975696	33.20562449815488	16.696711866628	14.982947737647509
7	30.927081046800236	30.297360509385165	19.614429275028495	19.161129168786104
8	27.547089465884035	33.89282898384401	19.156203392490394	19.40387815778156
9	27.09551355047544	16.13248359595972	18.692111526711102	38.07989132685374
10-11	26.584804892849483	24.712817279705668	26.503768205941235	22.198609621503614
12-13	27.005405223114288	21.74174201386945	26.717100656766384	24.53575210624988
14-15	24.16824579010986	23.403670525658544	23.996124298227368	28.431959386004223
16-17	24.56579270211245	26.44034039388225	24.683173462794382	24.310693441210912
18-19	24.566854983019624	25.909901475610813	25.902350306975663	23.620893234393897
20-21	25.080778428314687	25.113599015324983	25.806534207439086	23.999088348921248
22-23	24.911995815158317	24.76525998133768	25.616098183954612	24.706646019549392
24-25	24.607487603844632	25.024920875359808	25.691327581290725	24.67626393950483
26-27	24.530476507437466	25.19742688670121	25.733882482140054	24.538214123721268
28-29	24.547337152410577	25.339445464472593	25.604869036423615	24.508348346693214
30-31	24.873541850448337	25.200418524285755	25.412332461283377	24.513707163982527
32-33	24.617520095001748	25.245503598876194	25.392650305381615	24.74432600074044
34-35	24.815355176576926	25.18612065864944	25.524055279896896	24.47446888487674
36-37	24.852085353326938	25.05843930890517	25.509850465518834	24.579624872249052
38-39	24.784820621477056	25.1807430779063	25.48758370134095	24.54685259927569
40-41	24.878388717508265	25.06462205146147	25.45749280430715	24.59949642672311
42-43	24.735632400911364	24.943816848798082	25.417848157105148	24.902702593185406
44-45	24.622379680038385	25.047881792154726	25.525304162534923	24.804434365271966
46-47	24.802350090402257	24.906064064745237	25.483618705509635	24.80796713934287
48-49	24.663306193112746	25.03511555214976	25.43986516364584	24.86171309109165
50-51	24.5600097279741	25.163622924525736	25.54591665352166	24.730450693978504
52-53	24.609079514120996	25.10115953894811	25.5568798216962	24.732881125234698
54-55	24.36044781893444	24.917838148521362	25.82073771579	24.9009763167542
56-57	24.475567705092946	24.98788816370402	25.727347642826853	24.809196488376177
58-59	24.519256803708096	24.894284749962665	25.751625486523793	24.834832959805446
60-61	24.59111571991155	24.898319203630592	25.791274288430106	24.719290788027752
62-63	24.383614354986932	24.934224035846032	25.83167545751387	24.850486151653165
64-65	24.335201658657983	24.791149556839482	25.83602488033023	25.037623904172314
66-67	24.336982928095217	24.85433047405389	25.75159936655786	25.05708723129303
68-69	24.27927053773659	24.862850017046632	25.779385524797956	25.078493920418826
70-71	24.278584517580644	24.67288963593518	25.86861248521255	25.179913361271623
72-73	24.2848140827832	24.780252772446694	25.761022797520067	25.173910347250033
74-75	24.19753778049618	24.888224856049582	25.821549393043984	25.09268797041025
76-77	24.25507017499055	24.680686019461394	25.837542038156624	25.226701767391436
78-79	24.152392665281113	24.67363124037558	25.92019636255283	25.25377973179047
80-81	24.22865823058564	24.793904993289154	25.80387650203166	25.17356027409355
82-83	24.273204619585524	24.69781537292835	25.86119073821066	25.167789269275463
84-85	24.209772271905223	24.72427402100065	25.789198137902737	25.276755569191394
86-87	24.129151669295158	24.77872334551528	25.942801204160958	25.1493237810286
88-89	24.04916295918041	24.54854887380862	25.98742672570416	25.414861441306808
90-91	24.20887112587617	24.509437314716568	25.96844935076633	25.31324220864093
92-93	24.274331670351497	24.4939607607758	25.96458477322277	25.267122795649932
94-95	24.260068637117197	24.422756782315876	25.997849769157938	25.319324811408993
96-97	24.171677611796063	24.350745738503587	26.04672165260381	25.430854997096542
98	24.18129613003545	24.30118417776886	25.95154594722947	25.565973744966218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	142.5
2	235.0
3	376.0
4	432.5
5	503.5
6	803.5
7	1332.0
8	1888.0
9	2144.0
10	2184.0
11	2411.0
12	2789.5
13	3129.0
14	3451.5
15	3701.0
16	3808.0
17	3880.0
18	3818.5
19	3628.0
20	3499.0
21	3419.5
22	3453.0
23	3694.5
24	4169.0
25	4966.5
26	6101.0
27	7696.0
28	10329.5
29	14548.0
30	21138.0
31	30999.0
32	44610.5
33	60796.5
34	80128.0
35	103044.0
36	127419.0
37	151878.5
38	176640.0
39	199456.0
40	221685.5
41	245354.0
42	271146.0
43	298923.5
44	324536.0
45	348121.5
46	371431.0
47	393413.0
48	408356.0
49	415723.5
50	418329.0
51	419432.5
52	420145.5
53	417990.5
54	409901.0
55	393290.0
56	367614.5
57	335968.0
58	302637.5
59	264877.0
60	225094.5
61	186597.0
62	150242.0
63	115520.5
64	84693.0
65	61984.5
66	45886.0
67	34376.0
68	24977.5
69	17930.0
70	12730.5
71	8927.0
72	6417.0
73	4462.0
74	3190.0
75	2257.0
76	1500.0
77	898.0
78	506.5
79	290.5
80	157.5
81	111.0
82	83.0
83	64.5
84	59.0
85	36.0
86	25.5
87	20.5
88	17.5
89	20.5
90	18.5
91	15.0
92	17.0
93	17.0
94	11.0
95	7.5
96	8.0
97	9.0
98	7.5
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30688962388242674
2	0.15045115848653728
3	0.1473772154756055
4	0.14145872818590108
5	0.13640048614179318
6	0.0499056941812094
7	0.03976627021977775
8	0.015679403343073606
9	0.010632631235573687
10-11	0.0019556241916562174
12-13	0.006652563232613527
14-15	0.012100783121391845
16-17	0.007581628097857829
18-19	0.05231438086887981
20-21	0.1029025362782625
22-23	0.10225448485991309
24-25	0.07882140436986232
26-27	0.08890347864825421
28-29	0.10935437562023682
30-31	0.08442446840284804
32-33	0.022630184927493944
34-35	0.3263139615279974
36-37	0.42440485939922307
38-39	0.39335000603318665
40-41	0.3889283454708202
42-43	0.36332171199356217
44-45	0.34548022559988917
46-47	0.35107181969626694
48-49	0.3439375191261193
50-51	0.3452278869945142
52-53	0.3944396500109423
54-55	0.38020545868047095
56-57	0.36352243588420136
58-59	0.3428536101166676
60-61	0.3523220427865339
62-63	0.3512094589355624
64-65	0.3686953772943888
66-67	0.3916581903835134
68-69	0.34994776590868737
70-71	0.3236758761081679
72-73	0.30685521407260286
74-75	0.2917206327184071
76-77	0.2953049879083928
78-79	0.30233605904906885
80-81	0.2916403431621514
82-83	0.30241061363702054
84-85	0.2943759230431485
86-87	0.28966177909727925
88-89	0.3150791586205062
90-91	0.2964921263473161
92-93	0.28587096504835036
94-95	0.3022787093660291
96-97	0.3007589427654751
98	0.30014530115694954
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	8718444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.06033565469364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17992329772785	41.72372559278677
2	11.693843462663708	12.6434620537009
3	4.303201275390597	6.978975160119644
4	2.066187340941387	4.467951247070811
5	1.1934418613725457	3.2258933805081087
6	0.7543638975792792	2.4468699305351254
7	0.4999870107221054	1.8920625935836748
8	0.3638370451053999	1.5735322225607857
9	0.27853127904453573	1.3551744991930865
>10	1.5033553420319665	15.161488901404018
>50	0.11874144116802629	4.359073331568514
>100	0.04396650410185451	3.8815669766659484
>500	5.133832264808989E-4	0.1670621276884718
>1k	1.0685892425223235E-4	0.12316198261416204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.293987321590871E-5
2	0.0	0.0	0.0	0.0	2.293987321590871E-5
3	0.0	1.1469936607954356E-5	0.0	0.0	2.293987321590871E-5
4	0.0	1.1469936607954356E-5	0.0	1.1469936607954356E-5	2.293987321590871E-5
5	0.0	4.587974643181742E-5	0.0	1.1469936607954356E-5	3.440980982386307E-5
6	0.0	4.587974643181742E-5	0.0	1.1469936607954356E-5	5.734968303977178E-5
7	0.0	4.587974643181742E-5	0.0	1.1469936607954356E-5	5.734968303977178E-5
8	0.0	4.587974643181742E-5	0.0	1.1469936607954356E-5	6.881961964772613E-5
9	0.0	4.587974643181742E-5	0.0	2.293987321590871E-5	8.028955625568049E-5
10-11	0.0	4.587974643181742E-5	0.0	3.440980982386307E-5	8.602452455965768E-5
12-13	0.0	5.734968303977178E-5	0.0	4.0144778127840245E-5	9.749446116761202E-5
14-15	0.0	5.734968303977178E-5	0.0	5.734968303977178E-5	2.0645885894317838E-4
16-17	0.0	6.881961964772613E-5	0.0	6.881961964772613E-5	2.9248338350283605E-4
18-19	0.0	6.881961964772613E-5	0.0	8.602452455965768E-5	3.4409809823863065E-4
20-21	0.0	9.175949286363485E-5	0.0	9.749446116761202E-5	3.6130300315056216E-4
22-23	0.0	9.175949286363485E-5	0.0	1.7778401742329252E-4	4.014477812784024E-4
24-25	0.0	9.175949286363485E-5	0.0	4.4159255940624267E-4	4.7600236923010575E-4
26-27	0.0	1.032294294715892E-4	0.0	9.003900237244168E-4	4.817373375340829E-4
28-29	0.0	1.032294294715892E-4	0.0	0.0032804018698749456	4.817373375340829E-4
30-31	0.0	1.032294294715892E-4	0.0	0.0133682111165708	4.874723058380601E-4
32-33	0.0	1.032294294715892E-4	0.0	0.03670379714545394	5.333520522698775E-4
34-35	0.0	1.2043433438352073E-4	0.0	0.06732852788869206	5.734968303977178E-4
36-37	0.0	1.319042709914751E-4	0.0	0.11928734072272529	5.734968303977178E-4
38-39	0.0	1.3763923929545227E-4	0.0	0.20553552904623806	6.136416085255579E-4
40-41	0.0	1.5484414420738378E-4	0.0	0.30687241897751477	6.48051418349421E-4
42-43	1.1469936607954356E-5	1.7778401742329252E-4	0.0	0.4127456688372375	6.652563232613526E-4
44-45	1.1469936607954356E-5	1.835189857272697E-4	0.0	0.5415358520396529	6.939311647812384E-4
46-47	1.1469936607954356E-5	1.9498892233522404E-4	0.0	0.692566242324892	7.398109112130559E-4
48-49	1.1469936607954356E-5	2.007238906392012E-4	0.0	0.8553991973797159	7.914256259488505E-4
50-51	1.1469936607954356E-5	2.1792879555113277E-4	1.1469936607954356E-5	1.0336592171722385	7.914256259488505E-4
52-53	1.1469936607954356E-5	2.2939873215908712E-4	1.1469936607954356E-5	1.2210779813462127	8.430403406846451E-4
54-55	1.1469936607954356E-5	2.4086866876704146E-4	1.1469936607954356E-5	1.4337592808992063	8.946550554204397E-4
56-57	1.1469936607954356E-5	2.4086866876704146E-4	1.1469936607954356E-5	1.6798754456643867	9.003900237244168E-4
58-59	1.7204904911931534E-5	2.6380854198295014E-4	1.1469936607954356E-5	1.948713554849925	9.175949286363485E-4
60-61	4.587974643181742E-5	2.7527847859090454E-4	1.1469936607954356E-5	2.2375494985114317	9.290648652443028E-4
62-63	4.587974643181742E-5	2.7527847859090454E-4	1.1469936607954356E-5	2.5415372284320457	9.405348018522572E-4
64-65	4.587974643181742E-5	3.0968828841476757E-4	1.1469936607954356E-5	2.8717968481531795	9.577397067641886E-4
66-67	4.587974643181742E-5	3.2115822502272196E-4	1.1469936607954356E-5	3.2479247443695227	9.806795799800973E-4
68-69	4.587974643181742E-5	3.2115822502272196E-4	1.1469936607954356E-5	3.6584337755682093	9.864145482840745E-4
70-71	4.587974643181742E-5	3.326281616306763E-4	1.1469936607954356E-5	4.10861731749381	0.0010150893898039604
72-73	4.587974643181742E-5	3.498330665426078E-4	1.1469936607954356E-5	4.573080930496314	0.0011699335340113442
74-75	4.587974643181742E-5	3.55568034846585E-4	1.7204904911931534E-5	5.061195552784419	0.0011699335340113442
76-77	4.587974643181742E-5	3.55568034846585E-4	2.293987321590871E-5	5.592786969784976	0.0011756685023153214
78-79	4.587974643181742E-5	3.55568034846585E-4	2.293987321590871E-5	6.175408134754321	0.0011814034706192987
80-81	4.587974643181742E-5	3.9571281297442524E-4	2.293987321590871E-5	6.7967231308706	0.0012043433438352073
82-83	4.587974643181742E-5	4.014477812784024E-4	2.293987321590871E-5	7.436080337271192	0.0012616930268749792
84-85	4.587974643181742E-5	4.129177178863568E-4	2.293987321590871E-5	8.09575080140447	0.0012616930268749792
86	4.587974643181742E-5	4.2438765449431115E-4	2.293987321590871E-5	8.60988497488772	0.0012616930268749792
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13145	0.0	39.560234	1
GGTATCA	7790	0.0	34.617935	1
TCAACGC	16450	0.0	31.363937	4
CAACGCA	17020	0.0	30.096054	5
TATCAAC	17230	0.0	29.704626	2
ATCAACG	17415	0.0	29.415287	3
AACGCAG	18350	0.0	27.833609	6
GTACATG	27825	0.0	26.544884	1
TACATGG	29035	0.0	24.747944	2
ACATGGG	30415	0.0	22.914978	3
ACGCAGA	21990	0.0	22.637072	7
CGCAGAG	22990	0.0	21.211319	8
GAGTACA	21740	0.0	20.427197	1
CATGGGG	22425	0.0	20.32338	4
AGTACAT	20240	0.0	19.63495	2
GCAGAGT	25215	0.0	18.118568	9
AGAGTAC	20190	0.0	16.914654	10-11
GTATTAG	2015	0.0	16.215275	1
GTATAGG	2175	0.0	16.080343	1
GTATACG	535	0.0	15.483164	1
>>END_MODULE