FastQCFastQC Report
Mon 15 Aug 2016
SRR2921567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8718444
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131300.038.647641
GGTATCA79100.036.469231
TCAACGC164000.030.4660034
CAACGCA168800.029.4027165
TATCAAC174350.028.6831952
ATCAACG175600.028.5075363
AACGCAG183850.026.8878336
GTACATG275400.025.0458011
TACATGG284700.023.8544652
ACATGGG301900.021.9459673
ACGCAGA222600.021.6525767
CGCAGAG234950.020.06928
CATGGGG223650.019.5372094
AGTACAT207450.019.0929812
GAGTACA226100.019.0747181
GTATATA18750.017.0099891
GCAGAGT268650.016.6832839
AGAGTAC210350.016.34095610-11
GTATAGG18650.016.335471
GTGTAGC45950.015.3322771
Produced by FastQC (version 0.11.5)