##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8718444
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.053079540339997	31.0	30.0	34.0	26.0	34.0
2	30.039937172275238	31.0	30.0	34.0	26.0	34.0
3	30.222651427250092	31.0	30.0	34.0	26.0	34.0
4	33.87272958339814	35.0	35.0	37.0	30.0	37.0
5	33.38215592140065	35.0	33.0	37.0	28.0	37.0
6	33.276431666017466	35.0	33.0	37.0	27.0	37.0
7	33.089252508819236	35.0	33.0	37.0	26.0	37.0
8	32.98574676857476	35.0	33.0	37.0	26.0	37.0
9	34.30714276538336	37.0	34.0	39.0	27.0	39.0
10-11	34.10097271944397	37.0	33.5	39.0	26.0	39.0
12-13	33.922678404540996	37.0	33.0	39.0	25.0	39.0
14-15	34.79497448168503	38.0	33.0	40.0	25.0	41.0
16-17	34.64404192995906	38.0	33.0	40.0	25.0	41.0
18-19	34.50830039167539	38.0	33.0	40.0	24.0	41.0
20-21	34.36781660810117	38.0	33.0	40.0	23.5	41.0
22-23	34.19628187093936	38.0	33.0	40.0	22.5	41.0
24-25	34.04161826353418	38.0	33.0	40.0	21.5	41.0
26-27	33.95525629343952	38.0	32.5	40.0	20.5	41.0
28-29	33.97188236800053	38.0	33.0	40.0	20.5	41.0
30-31	33.82320841884171	38.0	33.0	40.0	19.0	41.0
32-33	33.65411890011566	38.0	32.0	40.0	18.5	41.0
34-35	33.45431203090827	38.0	32.0	40.0	18.0	41.0
36-37	33.28674428602168	38.0	32.0	40.0	16.0	41.0
38-39	33.10470153848554	38.0	32.0	40.0	16.0	41.0
40-41	33.12153097502261	38.0	32.0	40.0	15.5	41.0
42-43	32.922170114300215	38.0	31.5	40.0	14.5	41.0
44-45	32.718520644280105	37.0	31.0	40.0	12.0	41.0
46-47	32.459500456732876	37.0	31.0	40.0	9.0	41.0
48-49	32.169154725315664	37.0	30.5	40.0	8.5	41.0
50-51	31.898748503746766	37.0	30.0	40.0	7.5	41.0
52-53	31.57187417846579	36.0	30.0	39.5	4.0	41.0
54-55	31.252036946042207	36.0	29.5	39.0	2.0	40.5
56-57	30.909478457394464	35.5	29.0	39.0	2.0	40.0
58-59	30.435392198424395	35.0	28.0	39.0	2.0	40.0
60-61	30.08313989285244	35.0	28.0	38.5	2.0	40.0
62-63	29.749201463013357	34.5	28.0	38.0	2.0	40.0
64-65	29.29335400904106	34.0	27.0	38.0	2.0	40.0
66-67	28.872514120638957	34.0	26.0	37.0	2.0	39.5
68-69	28.440777677759932	34.0	26.0	36.5	2.0	39.0
70-71	27.972981073228205	33.0	26.0	36.0	2.0	39.0
72-73	27.498506556903962	33.0	25.5	35.5	2.0	38.5
74-75	26.96469536307167	32.5	24.5	35.0	2.0	37.5
76-77	25.470490032395688	30.5	22.0	34.0	2.0	36.0
78-79	26.34310141809708	32.0	24.0	35.0	2.0	36.0
80-81	26.398533155686955	32.0	24.0	35.0	2.0	36.0
82-83	26.080096402523203	32.0	23.5	35.0	2.0	36.0
84-85	25.65923678583013	32.0	21.0	35.0	2.0	35.0
86-87	25.216671117002072	32.0	20.0	34.0	2.0	35.0
88-89	24.773971708713162	31.5	18.5	34.0	2.0	35.0
90-91	24.346423226438112	31.0	14.5	34.0	2.0	35.0
92-93	23.934359732080633	31.0	7.5	34.0	2.0	35.0
94-95	23.546089474222693	31.0	2.0	34.0	2.0	35.0
96-97	23.007718579140956	31.0	2.0	34.0	2.0	35.0
98-99	22.24253398886315	30.5	2.0	34.0	2.0	35.0
100-101	19.66104846231736	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	228932.0
3	104117.0
4	54919.0
5	39418.0
6	33811.0
7	33539.0
8	37865.0
9	43645.0
10	49403.0
11	56147.0
12	61329.0
13	67281.0
14	71425.0
15	75541.0
16	79844.0
17	81628.0
18	81222.0
19	80354.0
20	81205.0
21	84736.0
22	91714.0
23	102506.0
24	118281.0
25	140094.0
26	165394.0
27	195862.0
28	229458.0
29	269234.0
30	314787.0
31	371764.0
32	442277.0
33	536476.0
34	667684.0
35	847136.0
36	1083616.0
37	1149017.0
38	522900.0
39	23883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.872306678564485	18.42344021938528	13.391196649386778	25.313056452663456
2	16.337835054053222	19.919185120647676	37.702863033816584	26.040116791482514
3	20.352322406486945	22.978160644656985	28.036362030570857	28.63315491828522
4	14.624663538442196	15.509466809954397	34.36182201791586	35.50404763368755
5	15.420366799242402	36.17007168726045	33.20266191581307	15.206899597684073
6	35.186037216284646	33.671851434320466	16.396313392628826	14.745797956766058
7	30.873728845291094	30.393521863206306	19.619250271120713	19.11349902038189
8	27.534166412595027	34.276614970842836	18.984573505432692	19.20464511112944
9	26.91979419800226	16.083520051479205	18.67763248472027	38.31905326579826
10-11	26.60623555043733	24.89908560939545	26.490512726639654	22.00416611352757
12-13	27.01640434389111	21.804787814014375	26.762451190122334	24.416356651972183
14-15	24.096979027289425	23.492805155806902	23.97517158490867	28.435044231995004
16-17	24.57886836395525	26.56361761735852	24.683740562262596	24.173773456423632
18-19	24.556073928226958	25.973214319023896	25.923114207763653	23.5475975449855
20-21	25.054967023802412	25.172472539727544	25.828180632109444	23.9443798043606
22-23	24.866095722094162	24.847642791674108	25.653849312853623	24.63241217337811
24-25	24.604885311037478	25.072657530347026	25.687268582487295	24.6351885761282
26-27	24.517633333683346	25.24458951473883	25.752264777233574	24.48551237434425
28-29	24.53136925563889	25.384104613247636	25.63771742276522	24.446808708348254
30-31	24.836051283407528	25.246957856576017	25.470057648885362	24.44693321113109
32-33	24.63571801657657	25.261470172182243	25.390105418825026	24.71270639241616
34-35	24.773360179048957	25.223966112524337	25.570974450822586	24.43169925760412
36-37	24.818651764745706	25.127519912750213	25.534980090835475	24.518848231668606
38-39	24.772476373859455	25.227959654523236	25.50428116762434	24.495282803992964
40-41	24.817096783612026	25.09848634728583	25.51937469393758	24.56504217516456
42-43	24.698275236456745	25.020008661480063	25.46670765829618	24.815008443767013
44-45	24.59519741742236	25.106315367122782	25.566491881119056	24.731995334335796
46-47	24.687584598909595	25.017544368910134	25.542277755598924	24.752593276581347
48-49	24.581275355473423	25.107873759299075	25.51777081791935	24.793080067308157
50-51	24.499994063305127	25.19173086662036	25.576250880251244	24.732024189823264
52-53	24.633971494527334	25.126777562896397	25.483401383353314	24.755849559222963
54-55	24.406063175409244	24.961909128312566	25.687492697445162	24.944534998833028
56-57	24.48286320654236	25.049478225967142	25.589095736596086	24.878562830894417
58-59	24.477326041996992	24.95069968507332	25.61649697539277	24.955477297536916
60-61	24.56073301393806	24.99701258923294	25.632437584340927	24.809816812488073
62-63	24.40863533764313	24.9744716624986	25.625793431856874	24.991099568001392
64-65	24.262979772600133	24.852028105869703	25.69380350360323	25.191188617926937
66-67	24.283713960609195	24.938104156938877	25.56834141749902	25.209840464952908
68-69	24.236431556027007	24.947607387780174	25.551105309925386	25.264855746267433
70-71	24.192463831438246	24.788581553745225	25.63127787677472	25.387676738041804
72-73	24.15538690158027	24.893821387418136	25.49706337917082	25.45372833183078
74-75	24.023639081426868	24.989425424744482	25.533594329686682	25.453341164141964
76-77	24.05969750852861	24.73207129683148	25.710154184019324	25.498077010620584
78-79	23.92495280364595	24.75264817290476	25.728888943988608	25.593510079460685
80-81	23.920611001928236	24.905654468298337	25.60765990558822	25.56607462418521
82-83	23.921788108744416	24.808439002200565	25.665580368094222	25.6041925209608
84-85	23.838936792612206	24.806151256830976	25.567750186648126	25.78716176390869
86-87	23.72172742226894	24.88468860465341	25.696763260666835	25.69682071241082
88-89	23.58125499659453	24.72672618146739	25.695294820593507	25.996724001344575
90-91	23.635003511747026	24.67688218052778	25.72210783493104	25.966006472794156
92-93	23.627986852947252	24.666625183396693	25.7125959310203	25.99279203263575
94-95	23.527476343507274	24.56899001493702	25.732339749646506	26.171193891909205
96-97	23.35681519919917	24.535856148470057	25.830331570568386	26.27699708176239
98-99	23.2555922790566	24.467383696402	25.80510419703964	26.47191982750176
100-101	23.497849874256975	24.25129288859797	25.862050474201098	26.388806762943958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	100.5
2	142.5
3	199.0
4	191.0
5	220.5
6	402.0
7	848.0
8	1246.5
9	1293.0
10	1079.5
11	923.0
12	957.5
13	1051.0
14	1185.0
15	1249.0
16	1326.5
17	1449.0
18	1627.5
19	1892.0
20	2054.0
21	2261.5
22	2546.5
23	2918.5
24	3396.0
25	3956.5
26	4948.5
27	6350.5
28	8235.0
29	11323.0
30	16352.0
31	23760.0
32	34909.5
33	49168.0
34	65344.5
35	84234.5
36	107895.0
37	132710.0
38	154990.0
39	177591.5
40	199997.0
41	221701.0
42	244254.0
43	270522.0
44	297456.0
45	322756.0
46	347352.5
47	369391.5
48	390592.5
49	406064.5
50	412464.5
51	415104.0
52	415588.5
53	414167.0
54	409070.5
55	393470.5
56	367934.5
57	335345.0
58	301517.0
59	267056.5
60	227808.0
61	188809.0
62	152623.0
63	117849.5
64	86734.5
65	63138.0
66	46224.0
67	34563.5
68	25507.0
69	18249.0
70	13050.5
71	9243.0
72	6542.0
73	4734.5
74	3438.0
75	2319.0
76	1547.0
77	896.5
78	440.0
79	226.0
80	131.0
81	76.0
82	41.5
83	27.0
84	17.0
85	9.0
86	5.0
87	2.5
88	0.5
89	1.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2414536355340471
2	0.0
3	1.032294294715892E-4
4	0.014360360633158853
5	0.016218490363647458
6	0.10789769367102661
7	0.13752453992937272
8	0.1554176410377815
9	0.11108633604803794
10-11	0.12121429007286164
12-13	0.09603777921840181
14-15	0.11971746334552358
16-17	0.07353949856189934
18-19	0.07898771845067766
20-21	0.05517613005256443
22-23	0.08132758551870035
24-25	0.07468649222269479
26-27	0.051700739260354255
28-29	0.018501007748630373
30-31	0.03254594512507048
32-33	0.04005301863497661
34-35	0.04537506922106743
36-37	0.03712244983164427
38-39	0.03916983351616412
40-41	0.021569215791258166
42-43	0.03261476474471821
44-45	0.01802500537940027
46-47	0.030791044824053464
48-49	4.0718274958237964E-4
50-51	0.016889481655212788
52-53	0.008195269706383386
54-55	0.016751842415917335
56-57	0.011160248319539589
58-59	0.008034690593872027
60-61	0.030997503682996648
62-63	0.013425560799610572
64-65	0.015283690530099177
66-67	0.059844394252001845
68-69	0.07816761798320894
70-71	0.034037036884104546
72-73	0.0566901616848144
74-75	0.04755435717657876
76-77	0.22352612461581448
78-79	0.07989957841101003
80-81	0.0162586351417753
82-83	0.010558076647621985
84-85	0.024964317027212655
86-87	0.17764637818399706
88-89	0.3802685433318147
90-91	0.2758921201994301
92-93	0.2772971874339045
94-95	0.001967094128264172
96-97	0.005861137606664676
98-99	0.005069711980715826
100-101	0.005339255491002752
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8718444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.1188908386061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98807464476567	37.08200236479651
2	13.174487593261441	13.205814110824813
3	4.8675378363760915	7.318667924223547
4	2.3797945041891273	4.770906438950783
5	1.398997465663625	3.5058100632540903
6	0.8743832084226096	2.629386994442576
7	0.6037775663815941	2.1182463358194856
8	0.41467349809251847	1.6626380627649495
9	0.306932512870841	1.3844805396653466
>10	1.7950395037468643	16.63233771001565
>50	0.14028024681277831	4.817609534451002
>100	0.05530876274074492	4.565789970708685
>500	5.977805712584776E-4	0.1886619879930943
>1k	1.1487610481437127E-4	0.11764796208946124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.587974643181742E-5
2	0.0	0.0	0.0	0.0	4.587974643181742E-5
3	0.0	0.0	0.0	0.0	4.587974643181742E-5
4	0.0	0.0	0.0	1.1469936607954356E-5	4.587974643181742E-5
5	0.0	1.1469936607954356E-5	0.0	3.440980982386307E-5	4.587974643181742E-5
6	0.0	1.1469936607954356E-5	0.0	3.440980982386307E-5	6.881961964772613E-5
7	0.0	1.1469936607954356E-5	0.0	3.440980982386307E-5	6.881961964772613E-5
8	1.1469936607954356E-5	1.1469936607954356E-5	0.0	3.440980982386307E-5	6.881961964772613E-5
9	1.1469936607954356E-5	1.1469936607954356E-5	0.0	6.881961964772613E-5	8.028955625568049E-5
10-11	1.7204904911931534E-5	1.1469936607954356E-5	0.0	9.175949286363485E-5	9.175949286363485E-5
12-13	2.293987321590871E-5	1.1469936607954356E-5	0.0	9.749446116761202E-5	9.175949286363485E-5
14-15	2.293987321590871E-5	2.293987321590871E-5	0.0	1.032294294715892E-4	1.3763923929545227E-4
16-17	2.293987321590871E-5	3.440980982386307E-5	0.0	1.261693026874979E-4	1.8925395403124686E-4
18-19	2.293987321590871E-5	4.587974643181742E-5	0.0	1.3763923929545227E-4	2.4086866876704146E-4
20-21	2.293987321590871E-5	4.587974643181742E-5	0.0	1.4337420759942944E-4	2.5807357367897297E-4
22-23	2.293987321590871E-5	4.587974643181742E-5	0.0	2.293987321590871E-4	3.2115822502272196E-4
24-25	2.293987321590871E-5	4.587974643181742E-5	0.0	4.874723058380601E-4	3.7277293975851656E-4
26-27	2.293987321590871E-5	4.587974643181742E-5	0.0	9.749446116761202E-4	4.18652686190334E-4
28-29	2.293987321590871E-5	4.587974643181742E-5	0.0	0.0033606914261306263	4.2438765449431115E-4
30-31	2.293987321590871E-5	4.587974643181742E-5	0.0	0.013494380419258298	4.2438765449431115E-4
32-33	2.293987321590871E-5	4.587974643181742E-5	0.0	0.036807026574925523	4.4159255940624267E-4
34-35	2.293987321590871E-5	5.734968303977178E-5	0.0	0.06775865051149035	4.702674009261286E-4
36-37	2.867484151988589E-5	7.45545879517033E-5	0.0	0.11988951239464289	4.932072741420373E-4
38-39	3.440980982386307E-5	9.749446116761202E-5	0.0	0.2061032909083318	5.104121790539687E-4
40-41	3.440980982386307E-5	1.1469936607954356E-4	0.0	0.30800220773339826	5.218821156619232E-4
42-43	3.440980982386307E-5	1.1469936607954356E-4	0.0	0.414122061230192	5.448219888778319E-4
44-45	3.440980982386307E-5	1.1469936607954356E-4	0.0	0.5430670885768148	5.620268937897634E-4
46-47	3.440980982386307E-5	1.261693026874979E-4	0.0	0.6942007082915254	5.964367036136264E-4
48-49	3.440980982386307E-5	1.261693026874979E-4	0.0	0.8559898991150257	6.193765768295351E-4
50-51	3.440980982386307E-5	1.261693026874979E-4	0.0	1.031216120674744	6.193765768295351E-4
52-53	3.440980982386307E-5	1.261693026874979E-4	0.0	1.2157960755382498	6.423164500454438E-4
54-55	3.440980982386307E-5	1.6631408081533815E-4	0.0	1.4251338885700247	6.881961964772613E-4
56-57	3.440980982386307E-5	1.9498892233522404E-4	0.0	1.666793982963015	7.1113606969317E-4
58-59	3.440980982386307E-5	2.064588589431784E-4	0.0	1.931325130952266	7.1113606969317E-4
60-61	3.440980982386307E-5	2.2939873215908712E-4	0.0	2.215682064368367	7.226060063011243E-4
62-63	3.440980982386307E-5	2.2939873215908712E-4	0.0	2.514829481040424	7.570158161249875E-4
64-65	3.440980982386307E-5	2.4086866876704146E-4	0.0	2.84148754066666	7.856906576448734E-4
66-67	4.0144778127840245E-5	2.6380854198295014E-4	0.0	3.2112840318754126	7.914256259488505E-4
68-69	4.587974643181742E-5	2.6380854198295014E-4	0.0	3.6158803107526984	7.914256259488505E-4
70-71	4.587974643181742E-5	2.6380854198295014E-4	0.0	4.059204830586742	8.143654991647592E-4
72-73	4.587974643181742E-5	2.6380854198295014E-4	0.0	4.518862540150513	9.290648652443028E-4
74-75	4.587974643181742E-5	2.6380854198295014E-4	0.0	5.003387072280329	9.290648652443028E-4
76-77	4.587974643181742E-5	2.6380854198295014E-4	0.0	5.534181328686633	9.290648652443028E-4
78-79	4.587974643181742E-5	2.695435102869273E-4	0.0	6.114233227855797	9.290648652443028E-4
80-81	4.587974643181742E-5	2.7527847859090454E-4	0.0	6.7329904281085025	9.347998335482799E-4
82-83	5.734968303977178E-5	2.7527847859090454E-4	0.0	7.371366954929114	9.864145482840745E-4
84-85	5.734968303977178E-5	2.7527847859090454E-4	0.0	8.029924835211421	9.97884484892029E-4
86-87	6.308465134374896E-5	2.7527847859090454E-4	0.0	8.724245977837331	9.97884484892029E-4
88-89	6.881961964772613E-5	2.7527847859090454E-4	0.0	9.472125989454081	0.0010093544214999832
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13130	0.0	38.64764	1
GGTATCA	7910	0.0	36.46923	1
TCAACGC	16400	0.0	30.466003	4
CAACGCA	16880	0.0	29.402716	5
TATCAAC	17435	0.0	28.683195	2
ATCAACG	17560	0.0	28.507536	3
AACGCAG	18385	0.0	26.887833	6
GTACATG	27540	0.0	25.045801	1
TACATGG	28470	0.0	23.854465	2
ACATGGG	30190	0.0	21.945967	3
ACGCAGA	22260	0.0	21.652576	7
CGCAGAG	23495	0.0	20.0692	8
CATGGGG	22365	0.0	19.537209	4
AGTACAT	20745	0.0	19.092981	2
GAGTACA	22610	0.0	19.074718	1
GTATATA	1875	0.0	17.009989	1
GCAGAGT	26865	0.0	16.683283	9
AGAGTAC	21035	0.0	16.340956	10-11
GTATAGG	1865	0.0	16.33547	1
GTGTAGC	4595	0.0	15.332277	1
>>END_MODULE