FastQCFastQC Report
Mon 15 Aug 2016
SRR2921566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730636
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91200.038.49861
TCAACGC114000.029.7821524
GGTATCA58000.029.2761251
CAACGCA117100.028.913975
TATCAAC121200.028.3924052
ATCAACG126500.026.9847283
AACGCAG127300.026.6762986
GTACATG224450.025.2994351
TACATGG237100.023.5820732
ACATGGG245400.022.0916023
ACGCAGA156250.020.7893967
CGCAGAG163400.019.596448
GAGTACA177450.019.1379591
CATGGGG190400.019.038234
AGTACAT165300.018.8720862
GTGTAGC36550.018.6332911
GTATAGG17450.016.8771761
GCAGAGT183000.016.4175789
GTATATG18700.016.241181
GTATATA15300.015.9404171
Produced by FastQC (version 0.11.5)