##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921566_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6730636
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.422727213297524	31.0	28.0	31.0	10.0	34.0
2	27.385045781706218	31.0	27.0	31.0	10.0	34.0
3	27.481989220632343	31.0	27.0	33.0	10.0	34.0
4	30.81165316323747	35.0	31.0	37.0	10.0	37.0
5	30.702444018663318	35.0	31.0	37.0	10.0	37.0
6	30.7843047818958	35.0	31.0	37.0	10.0	37.0
7	30.567076870595884	35.0	30.0	37.0	10.0	37.0
8	30.502040668965012	35.0	30.0	37.0	10.0	37.0
9	31.678946684978953	37.0	31.0	39.0	10.0	39.0
10-11	31.42750484501019	37.0	30.0	39.0	8.0	39.0
12-13	31.205337875945155	37.0	30.0	39.0	4.5	39.0
14-15	31.844386919750228	37.0	30.0	40.0	2.0	41.0
16-17	31.66781631037542	37.0	30.0	40.0	2.0	41.0
18-19	31.45183991527695	37.0	30.0	40.0	2.0	41.0
20-21	31.29114707733415	37.0	30.0	40.0	2.0	41.0
22-23	31.03770572350072	37.0	29.0	40.0	2.0	41.0
24-25	31.134949654089155	37.0	30.0	40.0	2.0	41.0
26-27	30.865885482441776	37.0	29.0	40.0	2.0	41.0
28-29	30.642419899100176	36.5	28.5	40.0	2.0	41.0
30-31	30.369710826733165	36.0	27.5	40.0	2.0	41.0
32-33	30.105694172140645	36.0	27.0	39.0	2.0	41.0
34-35	29.741559267207435	36.0	26.5	39.0	2.0	40.5
36-37	29.415248276685887	35.0	25.0	39.0	2.0	40.0
38-39	29.17297904685382	35.0	25.0	39.0	2.0	40.0
40-41	28.912523645610904	35.0	24.5	39.0	2.0	40.0
42-43	28.634608750198346	35.0	24.0	39.0	2.0	40.0
44-45	28.34318190732644	34.5	23.5	39.0	2.0	40.0
46-47	28.03052824428479	34.0	23.0	38.0	2.0	40.0
48-49	27.71765238827356	34.0	22.0	38.0	2.0	40.0
50-51	26.847458100542056	33.0	20.5	37.5	2.0	39.5
52-53	27.070535830492098	33.5	21.5	37.5	2.0	39.5
54-55	27.581952805054378	34.0	22.0	38.0	2.0	40.0
56-57	27.528188866549907	34.0	21.5	38.0	2.0	40.0
58-59	27.251650438383535	34.0	20.0	38.0	2.0	40.0
60-61	26.87684455079728	33.5	18.5	38.0	2.0	40.0
62-63	26.425279498103894	33.0	16.5	37.5	2.0	40.0
64-65	25.92422528866514	32.5	12.5	37.0	2.0	39.5
66-67	25.548268989735888	32.0	8.0	36.0	2.0	39.0
68-69	25.077668440248438	32.0	7.0	36.0	2.0	39.0
70-71	24.637089347871434	31.5	4.0	35.0	2.0	38.5
72-73	24.14724812038565	31.0	2.0	35.0	2.0	37.5
74-75	23.684000962167616	31.0	2.0	35.0	2.0	37.0
76-77	23.241172676698014	30.5	2.0	34.5	2.0	36.0
78-79	22.825009256183222	30.0	2.0	34.0	2.0	36.0
80-81	22.364819387053466	30.0	2.0	34.0	2.0	35.5
82-83	21.81628147770879	29.0	2.0	34.0	2.0	35.0
84-85	21.38003184245887	29.0	2.0	34.0	2.0	35.0
86-87	20.86001634912362	29.0	2.0	34.0	2.0	35.0
88-89	20.3201271766888	28.0	2.0	34.0	2.0	35.0
90-91	19.708604654894426	27.0	2.0	33.5	2.0	35.0
92-93	19.140369721375514	26.0	2.0	33.0	2.0	35.0
94-95	18.443655918994878	25.0	2.0	33.0	2.0	35.0
96-97	17.7814691657668	24.0	2.0	33.0	2.0	34.0
98	17.29588229106432	20.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	548291.0
3	87902.0
4	62782.0
5	51041.0
6	46787.0
7	48581.0
8	54748.0
9	62227.0
10	65718.0
11	67158.0
12	67579.0
13	65835.0
14	63973.0
15	65757.0
16	68710.0
17	72451.0
18	75723.0
19	77502.0
20	81163.0
21	84940.0
22	91469.0
23	101118.0
24	112096.0
25	126468.0
26	142425.0
27	161646.0
28	184445.0
29	209769.0
30	240386.0
31	276109.0
32	323061.0
33	382556.0
34	460023.0
35	557149.0
36	654673.0
37	607686.0
38	264853.0
39	15836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.510125096328544	16.516199662330376	12.585667386327106	25.388007855013978
2	16.59935522067555	18.180994409998053	38.72242513446046	26.497225234865933
3	21.265105773894724	21.413894484997634	26.73093347061572	30.590066270491928
4	14.151153652854651	14.119433815786303	33.9305252251558	37.79888730620325
5	16.024138041844353	35.475682240966236	32.54041553946696	15.959764177722446
6	36.4535106175187	32.07646539952712	16.24283997182813	15.227184011126047
7	32.20890715097359	29.322135296482664	18.60874722148525	19.8602103310585
8	28.27243234825425	33.700338147281	18.213546338184134	19.81368316628061
9	27.17786747325233	16.028572056894483	17.96496606503602	38.82859440481717
10-11	27.261331357843314	24.23202379859273	25.773807878841364	22.732836964722587
12-13	27.603221841169688	21.08610174246221	26.192258493780784	25.118417922587316
14-15	24.59420660904173	22.79184604807157	23.349013743415334	29.26493359947137
16-17	25.231423733099838	25.927414795458137	23.94908937894296	24.892072092499067
18-19	25.229646202625588	25.353911464475658	25.15545421483999	24.260988118058762
20-21	25.718915829396195	24.468961098481213	25.17696586997291	24.635157202149685
22-23	25.52375921119941	24.107792812459078	25.01154688212617	25.356901094215345
24-25	25.172233209706175	24.46982147639171	25.036842063786025	25.32110325011609
26-27	25.15544760717085	24.631298485132156	25.038823795510613	25.17443011218638
28-29	25.20350548748469	24.68968383220583	25.001448311922736	25.105362368386746
30-31	25.462585709731293	24.59854418363414	24.784387386572895	25.154482720061665
32-33	25.193506882969164	24.668200673540372	24.791974342338143	25.34631810115232
34-35	25.392577289469013	24.616030717542596	24.95229449966984	25.039097493318547
36-37	25.446598356528195	24.482515265339007	24.899724347329823	25.171162030802975
38-39	25.462201325193785	24.585943131295167	24.842554464092277	25.109301079418778
40-41	25.51502705558336	24.455840063204146	24.900093196881226	25.129039684331268
42-43	25.31722183672284	24.369442592746815	24.89628235861801	25.41705321191234
44-45	25.24432497547507	24.468785501516223	24.954312064477463	25.332577458531247
46-47	25.450104191181893	24.47604542113805	24.867389483284345	25.206460904395712
48-49	25.301150753681938	24.484577503382727	24.834627983405102	25.37964375953023
50-51	25.146295430635917	24.638280200987705	24.94573112829384	25.269693240082535
52-53	25.22563978225935	24.576988102036413	25.020753736325112	25.17661837937912
54-55	24.940389121678376	24.360469382349574	25.229569739806045	25.469571756166005
56-57	25.10336512223388	24.506719329397146	25.169332707456427	25.22058284091255
58-59	25.179187016644168	24.437326119363988	25.162430221327597	25.221056642664248
60-61	25.25651812254931	24.32324377209528	25.21605312395306	25.20418498140235
62-63	25.02035884040339	24.4415520130726	25.248704369658693	25.28938477686532
64-65	24.997725907594006	24.277539482717792	25.289898317499688	25.434836292188518
66-67	24.944369079962502	24.548870862732315	25.113226951333107	25.39353310597207
68-69	24.94346211702792	24.309355197903276	25.202950816421417	25.544231868647387
70-71	24.93168503975034	24.17837593954031	25.33837760297773	25.551561417731616
72-73	24.929568753921334	24.307587528632254	25.207888418784247	25.554955298662165
74-75	24.732239385964565	24.392659093467614	25.370750766810428	25.504350753757386
76-77	24.888060015641834	24.148384768815813	25.330239438354347	25.633315777188002
78-79	24.780886284401994	24.088288221295183	25.426177080288458	25.704648414014365
80-81	24.823072995027196	24.274481936719475	25.294344400419543	25.60810066783379
82-83	24.858484316925388	24.290789786043636	25.32538734353175	25.52533855349922
84-85	24.89911799914006	24.274905380167247	25.23410860290769	25.591868017785004
86-87	24.743438256658596	24.26327435276588	25.450504749487802	25.542782641087726
88-89	24.72201010753103	24.056958203540134	25.41219781977976	25.80883386914908
90-91	24.763744385865046	24.044494347866255	25.44401213577042	25.747749130498278
92-93	24.789446370416748	24.104172064761343	25.476382010327686	25.62999955449422
94-95	24.82712156189743	23.980288065408203	25.491229892445265	25.701360480249097
96-97	24.66903704688347	23.992972758261676	25.526460085092573	25.81153010976228
98	24.75986502937115	23.951132084553496	25.41004041876481	25.878962467310547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	69.5
2	102.0
3	188.5
4	236.0
5	261.0
6	394.0
7	689.0
8	967.0
9	1144.0
10	1254.5
11	1397.0
12	1690.0
13	1974.0
14	2198.5
15	2406.5
16	2520.5
17	2563.5
18	2487.0
19	2318.0
20	2138.0
21	1945.5
22	1867.0
23	1928.0
24	2096.5
25	2447.5
26	2948.5
27	3569.0
28	4520.0
29	6124.5
30	8898.5
31	13505.5
32	20956.5
33	31136.5
34	44117.5
35	59894.5
36	77945.5
37	96174.5
38	113546.5
39	129217.5
40	144154.5
41	161931.5
42	182806.0
43	205405.0
44	226856.5
45	248294.0
46	271294.0
47	292621.5
48	308606.0
49	320259.5
50	325655.0
51	328253.5
52	334453.5
53	340279.0
54	339775.0
55	332804.0
56	317598.5
57	295528.0
58	271577.5
59	243375.5
60	210420.5
61	174919.5
62	141018.5
63	108837.0
64	79705.0
65	57402.5
66	41353.0
67	29695.0
68	21147.0
69	15010.0
70	10549.5
71	7417.5
72	5356.0
73	3903.5
74	2770.0
75	1900.5
76	1254.5
77	767.5
78	455.5
79	278.5
80	176.5
81	115.5
82	81.5
83	59.5
84	48.0
85	38.5
86	28.0
87	20.0
88	23.5
89	21.5
90	15.0
91	18.5
92	17.5
93	13.5
94	12.5
95	10.5
96	9.5
97	10.0
98	6.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3049786082622801
2	0.14689547911965528
3	0.14405770866230175
4	0.13802558926080685
5	0.13318206481527153
6	0.0498912732764036
7	0.039684214092100656
8	0.015214015436282693
9	0.010459635612444352
10-11	0.002288045290222202
12-13	0.006641274316424183
14-15	0.01181166237484838
16-17	0.0066709891903231735
18-19	0.05191931342000964
20-21	0.10329633039136271
22-23	0.10340033245000918
24-25	0.078848417890969
26-27	0.08948634274680728
28-29	0.10833300151724146
30-31	0.08337993616056491
32-33	0.02106784559438365
34-35	0.3233869726427042
36-37	0.4213717693246225
38-39	0.39124831590952175
40-41	0.38647907864873393
42-43	0.36144429738883516
44-45	0.34433595874149187
46-47	0.349068052409906
48-49	0.3421964878207646
50-51	0.34263478221077476
52-53	0.39255577036107736
54-55	0.37862692322092595
56-57	0.361214007116118
58-59	0.34037645179445153
60-61	0.35070979919282513
62-63	0.34879318982634033
64-65	0.3664438249223402
66-67	0.3889825567747238
68-69	0.3477754553952999
70-71	0.32147036327621936
72-73	0.3052608995643205
74-75	0.2905743231397449
76-77	0.2939618187642297
78-79	0.3008705269457448
80-81	0.2902400308083813
82-83	0.3008482407903205
84-85	0.29271379406047215
86-87	0.28802627270290654
88-89	0.31326162936162344
90-91	0.2956035655471489
92-93	0.2847130642631692
94-95	0.3013831085205024
96-97	0.29981564892233065
98	0.2992139227258761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6730636.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.39881375566424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82500368639764	41.5576287738383
2	11.012531830745404	11.761122724165961
3	4.174546467832495	6.687474880504602
4	2.078320120291692	4.43919316112423
5	1.2171335867253819	3.2496744856656137
6	0.7479275626326126	2.396306677184807
7	0.5163173744326285	1.9299514721299038
8	0.3859692529363644	1.6488240202369429
9	0.27404314064680907	1.3170220765574634
>10	1.598663684642921	15.824870436860841
>50	0.11884804109040403	4.326045409109054
>100	0.049543802028515	4.395739089835344
>500	9.548717857477053E-4	0.3302103588084195
>1k	1.9657781134629578E-4	0.13593643397844288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4857436949494818E-5	1.1885949559595854E-4
2	0.0	0.0	0.0	1.4857436949494818E-5	1.4857436949494818E-4
3	0.0	0.0	0.0	1.4857436949494818E-5	1.4857436949494818E-4
4	0.0	0.0	0.0	1.4857436949494818E-5	1.4857436949494818E-4
5	1.4857436949494818E-5	0.0	0.0	1.4857436949494818E-5	1.4857436949494818E-4
6	1.4857436949494818E-5	0.0	0.0	1.4857436949494818E-5	2.525764281414119E-4
7	1.4857436949494818E-5	0.0	0.0	1.4857436949494818E-5	2.674338650909067E-4
8	1.4857436949494818E-5	0.0	0.0	1.4857436949494818E-5	2.8229130204040153E-4
9	1.4857436949494818E-5	0.0	0.0	1.4857436949494818E-5	3.8629336068686526E-4
10-11	2.9714873898989635E-5	0.0	0.0	2.9714873898989635E-5	4.011507976363601E-4
12-13	2.9714873898989635E-5	7.428718474747409E-6	0.0	5.942974779797927E-5	6.240123518787824E-4
14-15	2.9714873898989635E-5	1.4857436949494818E-5	0.0	7.428718474747409E-5	7.948728767979727E-4
16-17	4.457231084848445E-5	1.4857436949494818E-5	0.0	8.171590322222149E-5	0.0010400205864646373
18-19	4.457231084848445E-5	1.4857436949494818E-5	0.0	1.0400205864646373E-4	0.001144022645111101
20-21	4.457231084848445E-5	1.4857436949494818E-5	0.0	1.2628821407070594E-4	0.001218309829858575
22-23	4.457231084848445E-5	1.4857436949494818E-5	0.0	1.9314668034343263E-4	0.0013297406069797861
24-25	4.457231084848445E-5	1.4857436949494818E-5	0.0	3.565784867878756E-4	0.0014411713841009973
26-27	4.457231084848445E-5	1.4857436949494818E-5	0.0	6.462985073030245E-4	0.0014634575395252396
28-29	4.457231084848445E-5	1.4857436949494818E-5	0.0	0.0021691857946262434	0.0014708862579999869
30-31	4.457231084848445E-5	2.9714873898989635E-5	0.0	0.00785215542780801	0.001493172413424229
32-33	4.457231084848445E-5	6.685846627272668E-5	0.0	0.020532977864201836	0.0015228872873232187
34-35	4.457231084848445E-5	7.428718474747409E-5	0.0	0.037455598549676436	0.0015748883166464506
36-37	4.457231084848445E-5	7.428718474747409E-5	0.0	0.06668017702933274	0.0015748883166464506
38-39	4.457231084848445E-5	1.0400205864646373E-4	0.0	0.11908978586867572	0.0016417467829191773
40-41	4.457231084848445E-5	1.3371693254545335E-4	0.0	0.1807778640829782	0.0017011765307171566
42-43	4.457231084848445E-5	1.7086052491919042E-4	0.0	0.2442414060127453	0.0017383201230908936
44-45	4.457231084848445E-5	1.782892433939378E-4	0.0	0.321715510985886	0.001797749870888873
46-47	4.457231084848445E-5	1.782892433939378E-4	0.0	0.41584480277941044	0.0019686103958080634
48-49	4.457231084848445E-5	1.782892433939378E-4	0.0	0.522528628795258	0.0020800411729292746
50-51	5.2001029323231864E-5	2.0057539881818002E-4	0.0	0.6440401768867013	0.0021691857946262434
52-53	5.942974779797927E-5	2.2286155424242225E-4	0.0	0.7665100296613871	0.0022657591347979597
54-55	5.942974779797927E-5	2.525764281414119E-4	0.0	0.902596723400285	0.0023697611934444234
56-57	7.428718474747409E-5	2.674338650909067E-4	0.0	1.0652559431233541	0.0023920473488686657
58-59	7.428718474747409E-5	2.674338650909067E-4	0.0	1.248306995059605	0.0024291909412424025
60-61	7.428718474747409E-5	2.748625835656541E-4	0.0	1.4533099100887346	0.0025257642814141192
62-63	1.0400205864646373E-4	2.8229130204040153E-4	0.0	1.6644638040149549	0.0026446237770100774
64-65	1.0400205864646373E-4	2.8229130204040153E-4	0.0	1.8852601745213975	0.002652052495484825
66-67	1.0400205864646373E-4	2.9714873898989636E-4	0.0	2.1397680694662435	0.0026817673693838146
68-69	1.0400205864646373E-4	2.9714873898989636E-4	0.0	2.434502474951847	0.002704053524808057
70-71	1.0400205864646373E-4	2.9714873898989636E-4	0.0	2.759709483620864	0.0027857694280302783
72-73	1.0400205864646373E-4	3.0457745746464374E-4	0.0	3.094432680656033	0.0030977756039696695
74-75	1.0400205864646373E-4	3.120061759393912E-4	0.0	3.4401206661599293	0.0032092063810908807
76-77	1.0400205864646373E-4	3.26863612888886E-4	0.0	3.8187327319439053	0.003246349973464618
78-79	1.0400205864646373E-4	3.342923313636334E-4	0.0	4.2410479485148205	0.003276064847363607
80-81	1.0400205864646373E-4	3.417210498383808E-4	0.0	4.703663962811241	0.003350352032111081
82-83	1.0400205864646373E-4	3.417210498383808E-4	0.0	5.17721505070249	0.0035880710233029984
84-85	1.1885949559595854E-4	3.417210498383808E-4	0.0	5.659242306373425	0.003595499741777746
86	1.3371693254545335E-4	3.417210498383808E-4	0.0	6.033679432374593	0.003595499741777746
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9120	0.0	38.4986	1
TCAACGC	11400	0.0	29.782152	4
GGTATCA	5800	0.0	29.276125	1
CAACGCA	11710	0.0	28.91397	5
TATCAAC	12120	0.0	28.392405	2
ATCAACG	12650	0.0	26.984728	3
AACGCAG	12730	0.0	26.676298	6
GTACATG	22445	0.0	25.299435	1
TACATGG	23710	0.0	23.582073	2
ACATGGG	24540	0.0	22.091602	3
ACGCAGA	15625	0.0	20.789396	7
CGCAGAG	16340	0.0	19.59644	8
GAGTACA	17745	0.0	19.137959	1
CATGGGG	19040	0.0	19.03823	4
AGTACAT	16530	0.0	18.872086	2
GTGTAGC	3655	0.0	18.633291	1
GTATAGG	1745	0.0	16.877176	1
GCAGAGT	18300	0.0	16.417578	9
GTATATG	1870	0.0	16.24118	1
GTATATA	1530	0.0	15.940417	1
>>END_MODULE