FastQCFastQC Report
Mon 15 Aug 2016
SRR2921566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730636
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94700.038.6430241
GGTATCA60200.037.153191
TCAACGC116600.030.8295294
CAACGCA121000.029.7867265
TATCAAC124800.029.0308762
AACGCAG131150.027.6949966
ATCAACG130600.027.633763
GTACATG220550.025.3312021
TACATGG236100.022.8270652
ACGCAGA167750.021.3694137
ACATGGG243150.021.1701833
CGCAGAG176450.019.9928788
CATGGGG184950.018.2808024
GAGTACA177750.017.9106461
AGTACAT165900.017.6026842
GCAGAGT197600.016.5779789
TACACTG61300.016.5000575
AGAGTAC160350.016.0804125
CAGAGTA180250.015.3589974
GTATTAG14450.015.1490281
Produced by FastQC (version 0.11.5)