##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6730636
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.04707058887154	31.0	30.0	34.0	26.0	34.0
2	30.032101275421816	31.0	30.0	34.0	26.0	34.0
3	30.21551707743518	31.0	30.0	34.0	26.0	34.0
4	33.857267426139224	35.0	35.0	37.0	30.0	37.0
5	33.372166612486545	35.0	33.0	37.0	28.0	37.0
6	33.26269954280695	35.0	33.0	37.0	27.0	37.0
7	33.078055327906604	35.0	33.0	37.0	26.0	37.0
8	32.97570913060816	35.0	33.0	37.0	26.0	37.0
9	34.29657583622112	37.0	34.0	39.0	27.0	39.0
10-11	34.083263899577986	37.0	33.5	39.0	26.0	39.0
12-13	33.89902603557821	37.0	33.0	39.0	25.0	39.0
14-15	34.76714161930611	38.0	33.0	40.0	25.0	41.0
16-17	34.605921862361896	38.0	33.0	40.0	24.5	41.0
18-19	34.45519888462249	38.0	33.0	40.0	24.0	41.0
20-21	34.305339198257045	38.0	33.0	40.0	23.0	41.0
22-23	34.12209306817364	38.0	33.0	40.0	22.0	41.0
24-25	33.95705487564622	38.0	33.0	40.0	20.5	41.0
26-27	33.86242555681216	38.0	32.5	40.0	20.0	41.0
28-29	33.8789777073073	38.0	33.0	40.0	19.5	41.0
30-31	33.72252503329552	38.0	32.5	40.0	18.5	41.0
32-33	33.55039464324025	38.0	32.0	40.0	18.0	41.0
34-35	33.33956924724498	38.0	32.0	40.0	16.5	41.0
36-37	33.15931904503527	38.0	32.0	40.0	16.0	41.0
38-39	32.97031068089257	38.0	31.0	40.0	15.0	41.0
40-41	32.98377813032825	38.0	32.0	40.0	15.0	41.0
42-43	32.76965029753504	37.5	31.0	40.0	13.5	41.0
44-45	32.55752101287308	37.0	31.0	40.0	9.0	41.0
46-47	32.27433224735374	37.0	31.0	40.0	8.5	41.0
48-49	31.964385386462737	37.0	30.0	40.0	7.5	41.0
50-51	31.669687975995135	36.5	30.0	40.0	6.5	41.0
52-53	31.317473935598358	36.0	30.0	39.0	2.0	41.0
54-55	30.969573900594238	35.5	29.0	39.0	2.0	40.0
56-57	30.595484810053613	35.0	28.0	39.0	2.0	40.0
58-59	30.084182163468654	35.0	28.0	38.5	2.0	40.0
60-61	29.697150908175693	34.0	28.0	38.0	2.0	40.0
62-63	29.339632317064837	34.0	27.0	38.0	2.0	40.0
64-65	28.857024878481024	34.0	26.0	37.0	2.0	40.0
66-67	28.405919514886854	34.0	26.0	37.0	2.0	39.0
68-69	27.947571150779808	33.0	26.0	36.0	2.0	39.0
70-71	27.459935806957915	33.0	25.0	36.0	2.0	39.0
72-73	26.96911829729018	32.5	24.0	35.0	2.0	38.0
74-75	26.422647800297028	32.0	22.5	35.0	2.0	37.0
76-77	24.985033955186402	30.5	20.5	34.0	2.0	35.5
78-79	25.84535644179837	31.5	22.0	34.5	2.0	36.0
80-81	25.892444785307063	32.0	22.5	35.0	2.0	36.0
82-83	25.56636497650445	32.0	20.5	35.0	2.0	35.5
84-85	25.1325209831582	32.0	19.5	34.5	2.0	35.0
86-87	24.67267929806336	31.0	17.5	34.0	2.0	35.0
88-89	24.211342657662662	31.0	13.0	34.0	2.0	35.0
90-91	23.763190729672502	31.0	7.0	34.0	2.0	35.0
92-93	23.33049751910518	31.0	2.0	34.0	2.0	35.0
94-95	22.91906515223821	30.0	2.0	34.0	2.0	35.0
96-97	22.350061792080275	30.0	2.0	34.0	2.0	35.0
98-99	21.539423540360822	30.0	2.0	34.0	2.0	35.0
100-101	18.937951034642197	25.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	180055.0
3	80897.0
4	43327.0
5	31245.0
6	27435.0
7	27084.0
8	30588.0
9	35579.0
10	40628.0
11	45334.0
12	50297.0
13	54701.0
14	58705.0
15	62272.0
16	65237.0
17	66798.0
18	67393.0
19	66975.0
20	67835.0
21	70408.0
22	75846.0
23	83689.0
24	96763.0
25	112015.0
26	132731.0
27	155332.0
28	182442.0
29	212590.0
30	249845.0
31	293658.0
32	347730.0
33	421272.0
34	521340.0
35	660575.0
36	828051.0
37	831442.0
38	338648.0
39	13874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65063573470653	17.91866062912805	13.243599313479196	25.187104322686228
2	16.742964557881308	19.296913397188618	37.47339775914193	26.48672428578815
3	21.14875690722654	22.207364776442983	27.085004795983497	29.558873520346975
4	14.884971337563528	15.038826315650264	33.43498698458805	36.64121536219815
5	15.99046005706134	35.700026747503564	32.631924631478846	15.677588563956252
6	36.469198168646685	32.667899586722896	15.937405459850723	14.925496784779693
7	32.12336013494886	29.52654975681265	18.680829671252592	19.6692604369859
8	28.174248463251892	34.12468414244404	18.13091193606378	19.570155458240286
9	26.920655876594914	16.022846028926562	17.991359335448827	39.0651387590297
10-11	27.21015237064724	24.47893927383146	25.80914598744602	22.501762368075276
12-13	27.519262831861962	21.206960986844717	26.318045262951728	24.955730918341594
14-15	24.470259092205932	22.94619691769258	23.38476065751687	29.198783332584615
16-17	25.169105275709917	26.133139905901132	24.013774344706835	24.683980473682112
18-19	25.1804463881393	25.49275794493821	25.212006151960747	24.114789514961743
20-21	25.598049333778803	24.566546490543793	25.241170474060798	24.5942337016166
22-23	25.39657662796338	24.264436263583647	25.114673476494765	25.22431363195821
24-25	25.162225352845624	24.56108122976504	25.07431596085151	25.20237745653782
26-27	25.07120927443801	24.717368507465647	25.142360946717858	25.069061271378484
28-29	25.152123879392228	24.837095044657875	25.04921698627987	24.96156408967002
30-31	25.37123950709173	24.67955953750576	24.88777171283886	25.061429242563655
32-33	25.146660289303018	24.728643721732716	24.83596446651089	25.28873152245338
34-35	25.31932608915869	24.719495526354144	25.00855249814327	24.952625886343895
36-37	25.331605672387685	24.654036787406746	24.946575384814775	25.067782155390795
38-39	25.36098750253233	24.681788934993044	24.933067063138946	25.024156499335685
40-41	25.379128040800147	24.554177463531648	25.035505753960617	25.031188741707584
42-43	25.218277414553867	24.53011297204019	24.99717246646679	25.25443714693915
44-45	25.10392624971738	24.553506647646678	25.052272401531695	25.290294701104248
46-47	25.270027898969232	24.586575976686852	24.974429908075482	25.168966216268434
48-49	25.09890258138449	24.6148382027016	24.958603324838315	25.327655891075594
50-51	25.038394297360213	24.74651590790718	25.001994943171983	25.213094851560626
52-53	25.213637495594416	24.643006366034818	24.965565106687993	25.177791031682776
54-55	24.973803943759336	24.432429926338987	25.09702924128509	25.49673688861659
56-57	25.093998285266817	24.622000888570575	25.027355492123966	25.25664533403864
58-59	25.180818076146487	24.543896553204874	25.02499588788113	25.250289482767514
60-61	25.21744874946487	24.48320322708962	25.05233862415462	25.24700939929089
62-63	25.039373740943	24.542653140040052	25.060971903962397	25.357001215054552
64-65	24.987714754049154	24.399152284595097	25.10766214481448	25.50547081654127
66-67	24.939917872645218	24.66648955955036	24.917083245002768	25.476509322801654
68-69	24.976769044941037	24.43826587899622	24.99092433366246	25.59404074240029
70-71	24.914715587091017	24.324349630646	25.129217794089477	25.63171698817351
72-73	24.970374415506523	24.435192496244355	24.915058954225604	25.67937413402352
74-75	24.711618837193935	24.495391958690877	25.029615716980548	25.76337348713464
76-77	24.829949066431336	24.257917090951757	25.163607086281363	25.748526756335536
78-79	24.75667617355784	24.199310454244625	25.208648777890403	25.835364594307137
80-81	24.70526645677655	24.402712929075676	25.12176514547103	25.770255468676744
82-83	24.691183294767	24.386322777703235	25.15605955040169	25.76643437712808
84-85	24.76560121641193	24.369931477630942	25.003297309744482	25.861169996212645
86-87	24.489114618639007	24.36946617579897	25.23617693234877	25.905242273213254
88-89	24.50504418697048	24.236161072752502	25.1486992673088	26.110095472968215
90-91	24.483861906088936	24.23282960755336	25.17059342742184	26.112715058935866
92-93	24.504429098701337	24.26114209982477	25.206243445073223	26.02818535640067
94-95	24.448773648912926	24.128783300476762	25.18924441968821	26.233198630922104
96-97	24.242152066594606	24.132706315863764	25.318504815925053	26.306636801616577
98-99	24.276430407095166	24.09933657789911	25.24301741199782	26.381215603007906
100-101	24.60852950534943	23.736978006838182	25.318052481045967	26.33644000676642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	48.5
2	52.5
3	77.0
4	86.5
5	87.0
6	155.0
7	355.0
8	525.5
9	529.5
10	468.0
11	423.0
12	431.0
13	457.5
14	519.0
15	592.0
16	660.0
17	755.5
18	848.0
19	952.0
20	1016.5
21	1096.5
22	1255.5
23	1458.0
24	1662.5
25	2016.5
26	2436.5
27	2998.5
28	3851.5
29	5098.0
30	7252.0
31	10842.5
32	16735.0
33	25190.0
34	35645.0
35	48520.0
36	65785.0
37	83307.0
38	98803.0
39	114798.5
40	130249.0
41	144542.0
42	161116.5
43	182784.5
44	205290.0
45	227448.5
46	248675.0
47	268395.5
48	289179.0
49	306238.0
50	316756.0
51	324701.0
52	331673.0
53	336954.5
54	338776.5
55	334105.5
56	319776.0
57	296052.5
58	271109.0
59	245345.0
60	213045.0
61	176410.5
62	140829.5
63	108457.5
64	79240.0
65	56550.0
66	40522.5
67	28973.5
68	20664.0
69	14598.5
70	10383.0
71	7438.5
72	5243.0
73	3758.0
74	2753.5
75	1904.0
76	1235.0
77	747.0
78	418.0
79	211.0
80	111.5
81	66.0
82	38.0
83	22.0
84	13.0
85	5.5
86	3.5
87	4.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24069047858181605
2	0.0
3	5.942974779797927E-5
4	0.01369855686743422
5	0.015392304679676632
6	0.10848900460521116
7	0.13780272770656443
8	0.1550967843157764
9	0.11205478947308992
10-11	0.12104353882753428
12-13	0.09534017290490825
14-15	0.11900806996545349
16-17	0.07345516827830238
18-19	0.07875184455079728
20-21	0.05502451774245405
22-23	0.08103246112254474
24-25	0.0737077447064438
26-27	0.05136958825287834
28-29	0.01860151106076751
30-31	0.03213663612175729
32-33	0.03971392896599965
34-35	0.04560490271647434
36-37	0.03660872464355523
38-39	0.039119631488019856
40-41	0.02146899639202001
42-43	0.032047491500060324
44-45	0.018192931544656406
46-47	0.030784609359353263
48-49	3.417210498383808E-4
50-51	0.01665518682038369
52-53	0.00808244570052518
54-55	0.01623174986732309
56-57	0.010786499225333238
58-59	0.00785958414628276
60-61	0.031148616564615882
62-63	0.013327120943696851
64-65	0.015139728251535218
66-67	0.05957832216747422
68-69	0.07777125371213063
70-71	0.03368180956450475
72-73	0.05593082139637324
74-75	0.04771465876330261
76-77	0.2217843900635839
78-79	0.07932385587335283
80-81	0.016090604216302887
82-83	0.009999055067010012
84-85	0.02474506123938362
86-87	0.17510232316827115
88-89	0.3787457827165219
90-91	0.27562774156855313
92-93	0.27641518572687634
94-95	0.0017903211524141255
96-97	0.005363534738767629
98-99	0.004538946988070666
100-101	0.005044099844353491
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6730636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.567751473424515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11206057347296	34.777763270689206
2	13.105387114565833	12.46787594457375
3	5.0615470557825235	7.222992372615199
4	2.5896613196332368	4.927374642106174
5	1.4821193094901794	3.5250541483896205
6	0.9495186689591787	2.7099880838656234
7	0.6398540790339252	2.1305493867519876
8	0.47161666304355215	1.7946995374705184
9	0.3564800568857675	1.5261259276056978
>10	2.0079262272048966	17.728964720068912
>50	0.15175443576202374	4.935058853160214
>100	0.07047130588299466	5.699567567666387
>500	0.0013517227307729175	0.4003369175173185
>1k	2.514675521666125E-4	0.15364862751935995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.457231084848445E-5
2	0.0	0.0	0.0	0.0	5.942974779797927E-5
3	0.0	0.0	0.0	0.0	5.942974779797927E-5
4	0.0	0.0	0.0	0.0	7.428718474747409E-5
5	0.0	1.4857436949494818E-5	0.0	0.0	7.428718474747409E-5
6	0.0	1.4857436949494818E-5	0.0	0.0	1.3371693254545335E-4
7	0.0	1.4857436949494818E-5	0.0	1.4857436949494818E-5	1.4857436949494818E-4
8	0.0	1.4857436949494818E-5	0.0	1.4857436949494818E-5	2.0800411729292746E-4
9	0.0	1.4857436949494818E-5	0.0	2.9714873898989635E-5	2.525764281414119E-4
10-11	0.0	2.2286155424242225E-5	0.0	5.942974779797927E-5	2.525764281414119E-4
12-13	0.0	2.9714873898989635E-5	0.0	5.942974779797927E-5	4.5315182695959195E-4
14-15	0.0	2.9714873898989635E-5	0.0	7.428718474747409E-5	5.942974779797927E-4
16-17	1.4857436949494818E-5	2.9714873898989635E-5	0.0	1.1143077712121113E-4	7.725867213737305E-4
18-19	1.4857436949494818E-5	2.9714873898989635E-5	0.0	1.4857436949494818E-4	8.617313430706994E-4
20-21	1.4857436949494818E-5	2.9714873898989635E-5	0.0	1.4857436949494818E-4	9.285898093434261E-4
22-23	1.4857436949494818E-5	2.9714873898989635E-5	0.0	1.782892433939378E-4	0.0010251631495151424
24-25	1.4857436949494818E-5	4.457231084848445E-5	0.0	3.491497683131282E-4	0.001144022645111101
26-27	1.4857436949494818E-5	4.457231084848445E-5	0.0	6.314410703535298E-4	0.0012480247037575647
28-29	1.4857436949494818E-5	4.457231084848445E-5	0.0	0.0022137581054747276	0.0012925970146060492
30-31	1.4857436949494818E-5	4.457231084848445E-5	0.0	0.007985872360353464	0.0013223118885050387
32-33	1.4857436949494818E-5	4.457231084848445E-5	0.0	0.020867270195565474	0.0014486001025757447
34-35	1.4857436949494818E-5	5.942974779797927E-5	0.0	0.0378418919103633	0.0015748883166464506
36-37	1.4857436949494818E-5	8.91446216969689E-5	0.0	0.06728190322578728	0.0015748883166464506
38-39	1.4857436949494818E-5	1.0400205864646373E-4	0.0	0.11989951618242317	0.0016046031905454401
40-41	1.4857436949494818E-5	1.0400205864646373E-4	0.0	0.18227846521487717	0.0016343180644444299
42-43	1.4857436949494818E-5	1.0400205864646373E-4	0.0	0.24621744512702815	0.001671461656818167
44-45	2.9714873898989635E-5	1.0400205864646373E-4	0.0	0.32454585272476477	0.0017160339676666513
46-47	2.9714873898989635E-5	1.0400205864646373E-4	0.0	0.4187568604215114	0.0017754637154646308
48-49	3.7143592373737045E-5	1.0400205864646373E-4	0.0	0.5248686751148034	0.0019166093664848315
50-51	4.457231084848445E-5	1.1143077712121114E-4	0.0	0.6444858999951862	0.001946324240383821
52-53	4.457231084848445E-5	1.1885949559595854E-4	0.0	0.7647048510720236	0.0021023273283535164
54-55	4.457231084848445E-5	1.1885949559595854E-4	0.0	0.8990309385324061	0.002288045290222202
56-57	4.457231084848445E-5	1.1885949559595854E-4	0.0	1.0592981109066066	0.002362332474969676
58-59	4.457231084848445E-5	1.1885949559595854E-4	0.0	1.2405068406611202	0.002414333504292908
60-61	4.457231084848445E-5	1.3371693254545335E-4	0.0	1.4422262621244113	0.0024960494075151295
62-63	4.457231084848445E-5	1.3371693254545335E-4	0.0	1.6502378081358136	0.002600051466161593
64-65	4.457231084848445E-5	1.3371693254545335E-4	0.0	1.8687535620705087	0.0026594812139595723
66-67	4.457231084848445E-5	1.3371693254545335E-4	0.0	2.119428238282385	0.002711482243282804
68-69	4.457231084848445E-5	1.3371693254545335E-4	0.0	2.4096311254983926	0.002763483272606036
70-71	4.457231084848445E-5	1.3371693254545335E-4	0.0	2.7311832046778344	0.002845199175828258
72-73	5.942974779797927E-5	1.3371693254545335E-4	0.0	3.0634474958978615	0.0030457745746464374
74-75	5.942974779797927E-5	1.3371693254545335E-4	0.0	3.409239483460404	0.003149776633292901
76-77	5.942974779797927E-5	1.3371693254545335E-4	0.0	3.7878515492443805	0.003149776633292901
78-79	5.942974779797927E-5	1.3371693254545335E-4	0.0	4.211147356653963	0.003194348944141386
80-81	5.942974779797927E-5	1.3371693254545335E-4	0.0	4.67413480687412	0.0033132084397373444
82-83	5.942974779797927E-5	1.3371693254545335E-4	0.0	5.1488522035659035	0.0035434987124545137
84-85	7.428718474747409E-5	1.3371693254545335E-4	0.0	5.633732087131142	0.0035732135863535035
86-87	7.428718474747409E-5	1.3371693254545335E-4	0.0	6.144894776660037	0.0036846443634747147
88-89	7.428718474747409E-5	1.3371693254545335E-4	0.0	6.7095888115179605	0.0038480761699191575
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9470	0.0	38.643024	1
GGTATCA	6020	0.0	37.15319	1
TCAACGC	11660	0.0	30.829529	4
CAACGCA	12100	0.0	29.786726	5
TATCAAC	12480	0.0	29.030876	2
AACGCAG	13115	0.0	27.694996	6
ATCAACG	13060	0.0	27.63376	3
GTACATG	22055	0.0	25.331202	1
TACATGG	23610	0.0	22.827065	2
ACGCAGA	16775	0.0	21.369413	7
ACATGGG	24315	0.0	21.170183	3
CGCAGAG	17645	0.0	19.992878	8
CATGGGG	18495	0.0	18.280802	4
GAGTACA	17775	0.0	17.910646	1
AGTACAT	16590	0.0	17.602684	2
GCAGAGT	19760	0.0	16.577978	9
TACACTG	6130	0.0	16.500057	5
AGAGTAC	16035	0.0	16.080412	5
CAGAGTA	18025	0.0	15.358997	4
GTATTAG	1445	0.0	15.149028	1
>>END_MODULE