FastQCFastQC Report
Mon 15 Aug 2016
SRR2921565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468285
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54870.12279879193023721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113200.037.3207441
GGTATCA70150.034.3761831
GTACATG267600.028.9607621
TATCAAC146700.028.4354022
TCAACGC149400.028.0438824
TACATGG279300.027.5845412
CAACGCA152250.027.3976125
ATCAACG155050.026.9929433
AACGCAG160400.026.0654436
ACATGGG293300.025.6569773
CATGGGG241950.024.2660524
ATGGGGG170200.023.9143355
TGGGGGG155650.022.4676156
GTAAGTA47300.022.4364682
ATATGTA24500.021.9772741
ACGCAGA188500.021.6395957
TGTAAGT47700.021.3221
TATGTAA26600.020.7254162
CGCAGAG196350.020.6775058
TACCTGG34100.020.478252
Produced by FastQC (version 0.11.5)