##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921565_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4468285
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.737455421934815	31.0	28.0	31.0	16.0	34.0
2	27.69105641202385	31.0	28.0	33.0	15.0	34.0
3	27.774258580193518	31.0	28.0	33.0	16.0	34.0
4	31.15700856145031	35.0	32.0	37.0	16.0	37.0
5	31.064286185863256	35.0	32.0	37.0	16.0	37.0
6	31.159373227088246	35.0	32.0	37.0	16.0	37.0
7	30.96107074638256	35.0	32.0	37.0	11.0	37.0
8	30.900324173592328	35.0	32.0	37.0	11.0	37.0
9	32.0805416395776	37.0	32.0	39.0	10.0	39.0
10-11	31.831557073910908	37.0	31.0	39.0	10.0	39.0
12-13	31.586233644451955	37.0	31.0	39.0	10.0	39.0
14-15	32.220094734333195	37.0	31.0	40.0	8.0	41.0
16-17	32.039939260812595	37.0	31.0	40.0	7.5	41.0
18-19	31.852406012597676	37.0	30.0	40.0	2.0	41.0
20-21	31.704875919955867	37.0	30.0	40.0	2.0	41.0
22-23	31.492033968289846	37.0	30.0	40.0	2.0	41.0
24-25	31.580434887210643	37.0	30.0	40.0	2.0	41.0
26-27	31.31115741274337	37.0	30.0	40.0	2.0	41.0
28-29	31.08892349078002	37.0	30.0	40.0	2.0	41.0
30-31	30.810783555659498	36.0	29.0	40.0	2.0	41.0
32-33	30.533398943890106	36.0	28.5	39.5	2.0	41.0
34-35	30.1926479622495	36.0	27.5	39.0	2.0	41.0
36-37	29.883062629174283	35.0	27.0	39.0	2.0	40.0
38-39	29.6500962897398	35.0	26.0	39.0	2.0	40.0
40-41	29.4029642245291	35.0	26.0	39.0	2.0	40.0
42-43	29.134602201963393	35.0	25.0	39.0	2.0	40.0
44-45	28.85872376090603	35.0	24.5	39.0	2.0	40.0
46-47	28.53019547768327	34.5	24.0	38.5	2.0	40.0
48-49	28.214230515734783	34.0	23.0	38.0	2.0	40.0
50-51	27.311896958228942	33.0	22.5	37.5	2.0	39.5
52-53	27.52519803459269	33.5	23.0	37.5	2.0	39.5
54-55	28.076568750650416	34.0	23.0	38.0	2.0	40.0
56-57	28.043513450910137	34.0	23.0	38.5	2.0	40.0
58-59	27.780542646675404	34.0	22.5	38.0	2.0	40.0
60-61	27.399026136425945	34.0	22.0	38.0	2.0	40.0
62-63	26.92421644993549	33.0	20.5	37.5	2.0	40.0
64-65	26.462397317986657	33.0	18.5	37.0	2.0	39.5
66-67	26.109083574570555	33.0	18.0	36.0	2.0	39.0
68-69	25.644219874068014	32.0	14.5	36.0	2.0	39.0
70-71	25.208421799415213	32.0	10.0	35.0	2.0	38.5
72-73	24.72667533516774	31.5	7.5	35.0	2.0	37.5
74-75	24.27853180806506	31.0	4.5	35.0	2.0	37.0
76-77	23.856853020789856	31.0	2.0	35.0	2.0	36.0
78-79	23.462622795994434	31.0	2.0	34.0	2.0	36.0
80-81	23.03482700857264	30.5	2.0	34.0	2.0	35.0
82-83	22.493962224880462	30.0	2.0	34.0	2.0	35.0
84-85	22.086628874389167	30.0	2.0	34.0	2.0	35.0
86-87	21.5896094362826	29.0	2.0	34.0	2.0	35.0
88-89	21.087171028705647	29.0	2.0	34.0	2.0	35.0
90-91	20.485092490743092	29.0	2.0	34.0	2.0	35.0
92-93	19.909143888538892	27.0	2.0	34.0	2.0	35.0
94-95	19.205057980858427	26.5	2.0	33.0	2.0	35.0
96-97	18.558884337055495	25.0	2.0	33.0	2.0	35.0
98	18.094852723136505	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	330143.0
3	54098.0
4	40217.0
5	31641.0
6	28776.0
7	30318.0
8	33668.0
9	38195.0
10	39933.0
11	41797.0
12	41590.0
13	40779.0
14	39750.0
15	41418.0
16	43616.0
17	46998.0
18	48830.0
19	48502.0
20	50753.0
21	54184.0
22	58107.0
23	64674.0
24	72085.0
25	81945.0
26	93810.0
27	106548.0
28	122618.0
29	141043.0
30	160852.0
31	188069.0
32	221531.0
33	266367.0
34	322992.0
35	380857.0
36	449986.0
37	426417.0
38	175146.0
39	10032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.84444095265887	16.611618667085683	12.783160115827515	24.760780264427932
2	16.736228756817912	18.633735256762666	39.63938725596212	24.990648730457306
3	21.345149617074146	23.15329292925671	26.864960844590573	28.63659660907857
4	14.77980261565629	14.079478123683431	33.911898636595886	37.2288206240644
5	15.84099598570684	37.12004381347542	31.197314363030674	15.84164583778706
6	35.507939034911246	31.268913326333937	17.37882092574344	15.844326713011384
7	31.947880513313986	31.012093121558554	18.285702515568097	18.754323849559366
8	29.34310735569794	33.498156179177975	18.48082594219524	18.67791052292885
9	29.99107173432848	15.972485990411906	17.599673040599324	36.4367692346603
10-11	28.214095561054663	24.411390624548897	24.929485043309377	22.445028771087067
12-13	28.137674497720177	21.045847669064642	25.76982667310504	25.04665116011014
14-15	25.70746706283591	22.626434224993915	23.312377770876523	28.35372094129365
16-17	25.8395928888495	25.62108197259202	23.97020883537912	24.569116303179364
18-19	25.801975877160327	25.511179817629426	25.23722202996787	23.449622275242376
20-21	26.210103295169336	24.43565813272058	25.349058187808645	24.005180384301447
22-23	26.24128783746153	23.593536438463698	25.124665890484888	25.04050983358988
24-25	25.656638776804463	23.886903777343687	24.95232391316496	25.504133532686886
26-27	25.1286057402887	24.315358041449738	25.291038001846864	25.264998216414696
28-29	25.06833388539623	25.521189749485924	24.551896506858927	24.858579858258924
30-31	26.680486586614165	24.488707759089902	24.582400992704784	24.24840466159115
32-33	26.051839537689897	24.33342074622404	24.68167325509152	24.933066460994542
34-35	25.909911261053075	24.80133955408143	24.940764179411428	24.347985005454063
36-37	25.688375034440664	24.826201063356887	25.13136893827469	24.35405496392776
38-39	26.0157831671903	24.871501860930064	24.636651622187557	24.476063349692083
40-41	26.079306089965904	24.448534388007094	24.58639306853788	24.885766453489122
42-43	25.54146122125285	24.568776594388737	24.27691281111315	25.61284937324527
44-45	24.915221105534975	24.542073831601826	25.20360913378835	25.339095929074844
46-47	25.139687825976626	24.08385199524362	24.838326822690053	25.938133356089704
48-49	25.045887360732365	24.591044475821953	24.968281919728668	25.394786243717014
50-51	25.287830863319115	24.29000447882238	25.316911209170883	25.10525344868762
52-53	25.34255231170365	24.48425765195537	24.978277526656438	25.194912509684542
54-55	24.38467784226974	23.907246141105794	25.947599211659817	25.76047680496465
56-57	24.959201888773993	24.120252124017274	25.10112496327833	25.8194210239304
58-59	25.13823450554218	24.392215035045904	25.37843829140714	25.091112168004777
60-61	26.10786915029436	24.174306827356133	25.374526010606584	24.343298011742924
62-63	25.546093652627643	24.137553910951066	25.51231825230269	24.8040341841186
64-65	24.97310862498318	23.761755781617552	25.704280001823832	25.56085559157544
66-67	25.19254753830647	24.115494487015614	25.224078549173605	25.467879425504307
68-69	24.58788218156977	24.19933552728276	25.53745454333136	25.675327747816105
70-71	24.78704801093267	23.96478538215736	25.532057241602423	25.716109365307542
72-73	24.57344257985995	24.82764356283626	24.983358359861004	25.61555549744278
74-75	24.518657860796615	24.540439495254105	25.44340663416292	25.497496009786364
76-77	24.367364440928633	23.758556556344693	25.486830279062943	26.38724872366373
78-79	24.30552017161467	23.945370886425145	25.84254618923	25.90656275273019
80-81	24.84112693214675	24.235843828924537	25.126643970140055	25.796385268788658
82-83	24.859642032611195	24.359824575127103	25.34792221949253	25.43261117276917
84-85	24.828822580808062	24.623658810023198	24.86852662965594	25.6789919795128
86-87	24.22354477345637	24.95817714755354	25.148899229705425	25.66937884928467
88-89	24.0458920510432	24.179081995101402	25.552164134549905	26.222861819305493
90-91	24.97717922929426	23.563054569417453	25.746576435497744	25.71318976579055
92-93	25.2103222253305	23.741306776733417	25.346862091032634	25.70150890690344
94-95	24.920229090178978	24.094321981216364	25.107003734931745	25.878445193672917
96-97	24.224718919539516	23.968758126084623	25.507564294058515	26.298958660317346
98	24.19127268972731	23.766264665414198	25.201730109242888	26.8407325356156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	113.0
2	174.0
3	267.0
4	275.5
5	308.0
6	478.5
7	825.0
8	1171.5
9	1399.5
10	1434.0
11	1410.5
12	1576.5
13	1803.5
14	2076.0
15	2367.0
16	2556.0
17	2635.5
18	2676.0
19	2600.5
20	2465.0
21	2403.5
22	2394.5
23	2400.5
24	2478.0
25	2820.0
26	3315.0
27	3811.0
28	4623.0
29	6138.5
30	8548.0
31	12165.0
32	17827.0
33	24418.0
34	32241.5
35	41766.5
36	51082.5
37	59725.5
38	68459.0
39	75785.5
40	82571.5
41	90836.5
42	101719.0
43	117120.0
44	132815.5
45	149214.0
46	170510.5
47	193279.5
48	211583.5
49	226971.5
50	234952.0
51	237703.5
52	239902.5
53	239614.5
54	239763.5
55	233757.5
56	220759.0
57	205680.5
58	183930.5
59	156553.0
60	131057.5
61	107771.0
62	86795.5
63	67597.5
64	50724.0
65	37740.0
66	27853.5
67	20569.0
68	14988.0
69	10845.5
70	7777.0
71	5608.5
72	4185.5
73	3028.0
74	2116.5
75	1462.5
76	999.0
77	635.5
78	391.5
79	243.0
80	151.0
81	92.0
82	56.0
83	44.5
84	37.0
85	29.5
86	20.0
87	15.0
88	14.0
89	10.5
90	8.0
91	8.5
92	8.0
93	9.0
94	9.5
95	6.0
96	4.0
97	5.0
98	6.5
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2995556460700246
2	0.1415308110382395
3	0.13862141738944586
4	0.1326012105315574
5	0.12825950000951147
6	0.04896733310431183
7	0.039813933086184077
8	0.014770767755413989
9	0.010115737917344127
10-11	0.0018799158961435988
12-13	0.006322336198340079
14-15	0.01113402569442191
16-17	0.0070720645616830615
18-19	0.05076891917144945
20-21	0.10123570900244724
22-23	0.10191829751235652
24-25	0.07878861800444689
26-27	0.08868055641034536
28-29	0.10571169923136058
30-31	0.08314151850206511
32-33	0.021943542097247602
34-35	0.31926119305281553
36-37	0.41633423114237345
38-39	0.38382735210489033
40-41	0.380179420068326
42-43	0.35506911488412224
44-45	0.3375679930890711
46-47	0.3428608515347611
48-49	0.33545308770590954
50-51	0.33806035201425155
52-53	0.3860429672682025
54-55	0.37270451638604074
56-57	0.3552369645177065
58-59	0.3353188079990421
60-61	0.34442744811488074
62-63	0.34330845055765247
64-65	0.36063053274354706
66-67	0.38295453401025226
68-69	0.3419768434645507
70-71	0.3158258705521246
72-73	0.2986716379998142
74-75	0.28414704970699045
76-77	0.2877278418901211
78-79	0.295146795694545
80-81	0.2838673003176834
82-83	0.2944865871357803
84-85	0.28691097367334445
86-87	0.2816964450566604
88-89	0.30760123850649634
90-91	0.28901468908093375
92-93	0.2784849220674151
94-95	0.29504608591439446
96-97	0.29341234948084105
98	0.29261786121520894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4468285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.68379351031048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97836843095915	40.81901685766916
2	10.31181663773989	10.659076036422652
3	3.8711297918065726	6.002240184340262
4	1.9358574424421804	4.002098252823175
5	1.1070742306200043	2.8608897967975073
6	0.7287848102141876	2.259981818673653
7	0.4937956459393749	1.7864862540714725
8	0.37274817863267523	1.5412031916636408
9	0.27777451047273144	1.2920796397530203
>10	1.7227622157460178	16.642035146024455
>50	0.13363470447275383	4.768399942222789
>100	0.0624479679379199	5.474936376927957
>500	0.0027119180263170677	0.918785847766148
>1k	0.0010497743905932137	0.8487271367605927
>5k	4.3740599608037536E-5	0.12404351808338321
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5487	0.12279879193023721	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.4759902289133304E-5
9	0.0	0.0	0.0	2.2379951144566652E-5	4.4759902289133304E-5
10-11	0.0	0.0	0.0	3.3569926716849977E-5	6.713985343369995E-5
12-13	0.0	0.0	0.0	8.951980457826661E-5	1.1189975572283325E-4
14-15	0.0	0.0	0.0	1.1189975572283325E-4	1.4546968243968323E-4
16-17	0.0	0.0	0.0	1.2308973129511659E-4	1.6784963358424987E-4
18-19	0.0	0.0	0.0	1.342797068673999E-4	1.7903960915653322E-4
20-21	0.0	0.0	0.0	1.4546968243968323E-4	1.7903960915653322E-4
22-23	0.0	0.0	0.0	2.685594137347998E-4	1.7903960915653322E-4
24-25	0.0	0.0	0.0	3.804591694576331E-4	1.7903960915653322E-4
26-27	0.0	0.0	0.0	9.063880213549494E-4	2.1260953587338318E-4
28-29	0.0	0.0	0.0	0.002808683868643115	2.237995114456665E-4
30-31	0.0	2.2379951144566652E-5	0.0	0.01086546628068711	2.3498948701794985E-4
32-33	0.0	2.2379951144566652E-5	0.0	0.02964224529097853	3.356992671684998E-4
34-35	0.0	2.2379951144566652E-5	0.0	0.05602820769042261	3.5807921831306643E-4
36-37	0.0	2.2379951144566652E-5	0.0	0.10017266132308034	3.804591694576331E-4
38-39	0.0	2.2379951144566652E-5	0.0	0.18123284436870074	3.916491450299164E-4
40-41	0.0	2.2379951144566652E-5	0.0	0.2799619988429565	4.028391206021997E-4
42-43	0.0	2.2379951144566652E-5	0.0	0.37286117604405267	4.2521907174676636E-4
44-45	0.0	2.2379951144566652E-5	0.0	0.481090619779177	4.47599022891333E-4
46-47	0.0	2.2379951144566652E-5	0.0	0.6028711239323364	4.47599022891333E-4
48-49	0.0	2.2379951144566652E-5	0.0	0.7398028550103675	4.47599022891333E-4
50-51	0.0	2.2379951144566652E-5	0.0	0.8992600069154049	4.47599022891333E-4
52-53	0.0	2.2379951144566652E-5	0.0	1.0701868837820327	4.923589251804663E-4
54-55	0.0	2.2379951144566652E-5	0.0	1.2573392252284714	5.371188274695996E-4
56-57	0.0	2.2379951144566652E-5	0.0	1.4630445461737558	5.48308803041883E-4
58-59	0.0	2.2379951144566652E-5	0.0	1.701637205325981	5.706887541864496E-4
60-61	0.0	2.2379951144566652E-5	0.0	1.973128392660719	5.818787297587329E-4
62-63	0.0	2.2379951144566652E-5	0.0	2.273623996678815	6.042586809032996E-4
64-65	0.0	2.2379951144566652E-5	0.0	2.565839018773422	6.266386320478662E-4
66-67	0.0	2.2379951144566652E-5	0.0	2.8971406255420145	6.602085587647163E-4
68-69	0.0	2.2379951144566652E-5	0.0	3.2652124920411296	6.713985343369996E-4
70-71	0.0	2.2379951144566652E-5	0.0	3.6715205050707374	6.713985343369996E-4
72-73	0.0	3.3569926716849977E-5	0.0	4.0841396643231125	7.385383877706995E-4
74-75	0.0	4.4759902289133304E-5	1.1189975572283326E-5	4.499869636784583	7.385383877706995E-4
76-77	0.0	4.4759902289133304E-5	2.2379951144566652E-5	4.9629108259656665	7.497283633429828E-4
78-79	0.0	4.4759902289133304E-5	2.2379951144566652E-5	5.482830660980667	7.609183389152662E-4
80-81	0.0	4.4759902289133304E-5	2.2379951144566652E-5	6.024772367921921	7.832982900598328E-4
82-83	0.0	4.4759902289133304E-5	2.2379951144566652E-5	6.5910746516840355	8.728180946380994E-4
84-85	0.0	5.594987786141663E-5	2.2379951144566652E-5	7.165556807589489	8.728180946380994E-4
86	0.0	6.713985343369995E-5	2.2379951144566652E-5	7.615628815082297	8.728180946380994E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11320	0.0	37.320744	1
GGTATCA	7015	0.0	34.376183	1
GTACATG	26760	0.0	28.960762	1
TATCAAC	14670	0.0	28.435402	2
TCAACGC	14940	0.0	28.043882	4
TACATGG	27930	0.0	27.584541	2
CAACGCA	15225	0.0	27.397612	5
ATCAACG	15505	0.0	26.992943	3
AACGCAG	16040	0.0	26.065443	6
ACATGGG	29330	0.0	25.656977	3
CATGGGG	24195	0.0	24.266052	4
ATGGGGG	17020	0.0	23.914335	5
TGGGGGG	15565	0.0	22.467615	6
GTAAGTA	4730	0.0	22.436468	2
ATATGTA	2450	0.0	21.977274	1
ACGCAGA	18850	0.0	21.639595	7
TGTAAGT	4770	0.0	21.322	1
TATGTAA	2660	0.0	20.725416	2
CGCAGAG	19635	0.0	20.677505	8
TACCTGG	3410	0.0	20.47825	2
>>END_MODULE