FastQCFastQC Report
Mon 15 Aug 2016
SRR2921565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468285
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52680.11789758262957713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109100.038.1676981
GGTATCA67850.037.384371
ATATGTA22300.028.3830781
TATCAAC144700.028.193592
GTACATG273350.028.0819971
TCAACGC148200.027.4014074
TACATGG283750.026.7632182
CAACGCA152050.026.582645
ATCAACG155250.026.2488733
TATGTAA26200.025.5589852
AACGCAG162450.025.0809886
ACATGGG296500.025.0374013
GTAAGTA47200.023.947532
CATGGGG243450.023.0601774
TGTAAGT47350.023.0159111
ATGGGGG169750.022.663675
GTATAGG10100.022.6168751
TACCGTA2755.2750693E-1122.4485237
TGGGGGG147200.022.0015686
TAAGTAC68200.021.561891
Produced by FastQC (version 0.11.5)