FastQCFastQC Report
Mon 15 Aug 2016
SRR2921564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8544377
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159550.041.304271
GGTATCA89550.035.921991
TCAACGC205000.031.6876454
TATCAAC206950.031.3901652
CAACGCA212350.030.5897735
ATCAACG215300.030.1725833
AACGCAG226000.029.0330496
GTACATG323100.025.2534391
ACGCAGA262800.024.2110967
TACATGG332700.024.0825982
CGCAGAG275100.022.9588818
ACATGGG351900.022.3246143
GCAGAGT295800.019.8611779
GTATATA17450.019.7794691
CATGGGG261100.019.6183454
AGTACAT239900.019.1313172
GAGTACA258750.019.0483721
GTATAGG20150.017.5858941
AGAGTAC242100.017.33412610-11
ATGGGGG139450.015.845495
Produced by FastQC (version 0.11.5)