##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921564_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8544377
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.03506130405997	31.0	28.0	31.0	16.0	34.0
2	28.008084147036115	31.0	28.0	33.0	16.0	34.0
3	28.098976554990493	31.0	28.0	34.0	16.0	34.0
4	31.504617364144863	35.0	32.0	37.0	17.0	37.0
5	31.44038190262438	35.0	32.0	37.0	17.0	37.0
6	31.536378720180537	35.0	32.0	37.0	17.0	37.0
7	31.34563678545551	35.0	32.0	37.0	17.0	37.0
8	31.300510967622333	35.0	32.0	37.0	17.0	37.0
9	32.52757058823598	37.0	32.0	39.0	17.0	39.0
10-11	32.30695713683981	37.0	32.0	39.0	15.0	39.0
12-13	32.118349295683	37.0	32.0	39.0	11.0	39.0
14-15	32.82477932563135	38.0	32.0	40.0	10.0	41.0
16-17	32.68799843452601	38.0	31.5	40.0	10.0	41.0
18-19	32.51539392515101	38.0	31.0	40.0	10.0	41.0
20-21	32.393506805703915	37.5	31.0	40.0	9.5	41.0
22-23	32.17648583389989	37.0	31.0	40.0	8.0	41.0
24-25	32.27404636991088	37.0	31.0	40.0	8.0	41.0
26-27	32.03492074378272	37.0	30.5	40.0	4.5	41.0
28-29	31.846559731622328	37.0	30.0	40.0	2.0	41.0
30-31	31.602366854833303	37.0	30.0	40.0	2.0	41.0
32-33	31.362835991436242	37.0	30.0	40.0	2.0	41.0
34-35	31.027057385225394	36.5	29.5	40.0	2.0	41.0
36-37	30.732908262357803	36.0	29.0	40.0	2.0	41.0
38-39	30.517746115369206	36.0	28.0	39.0	2.0	41.0
40-41	30.29557585064423	36.0	28.0	39.0	2.0	40.0
42-43	30.0471809120782	35.5	27.0	39.0	2.0	40.0
44-45	29.78307482219008	35.0	27.0	39.0	2.0	40.0
46-47	29.48569778697733	35.0	26.0	39.0	2.0	40.0
48-49	29.196492851380505	35.0	26.0	39.0	2.0	40.0
50-51	28.256193400642317	34.0	24.5	38.0	2.0	39.5
52-53	28.458540277424557	34.0	25.0	38.0	2.0	39.5
54-55	29.03148362952618	35.0	26.0	39.0	2.0	40.0
56-57	29.009456570092823	35.0	26.0	39.0	2.0	40.0
58-59	28.768765645523366	34.5	26.0	39.0	2.0	40.0
60-61	28.422225049292653	34.0	25.5	38.0	2.0	40.0
62-63	27.998330715042187	34.0	24.5	38.0	2.0	40.0
64-65	27.53514773517133	34.0	23.0	37.5	2.0	40.0
66-67	27.187011352612366	33.0	22.0	37.0	2.0	39.5
68-69	26.728735459589387	33.0	21.5	36.0	2.0	39.0
70-71	26.304750246858255	33.0	20.0	36.0	2.0	39.0
72-73	25.83220725162291	32.0	19.0	35.0	2.0	38.0
74-75	25.38584790909858	32.0	17.5	35.0	2.0	37.0
76-77	24.95825541171697	32.0	13.5	35.0	2.0	37.0
78-79	24.555853516294988	31.0	8.5	35.0	2.0	36.0
80-81	24.112919584423768	31.0	4.5	34.5	2.0	36.0
82-83	23.57445352657075	31.0	2.0	34.0	2.0	35.0
84-85	23.15471484930967	30.5	2.0	34.0	2.0	35.0
86-87	22.656858422796653	30.0	2.0	34.0	2.0	35.0
88-89	22.139488168651734	30.0	2.0	34.0	2.0	35.0
90-91	21.549670970744852	29.0	2.0	34.0	2.0	35.0
92-93	21.001243741936946	29.0	2.0	34.0	2.0	35.0
94-95	20.319331766376884	29.0	2.0	34.0	2.0	35.0
96-97	19.67572047675331	27.5	2.0	34.0	2.0	35.0
98	19.194051128596033	27.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	559536.0
3	89738.0
4	65033.0
5	51849.0
6	47847.0
7	49786.0
8	56910.0
9	64516.0
10	67666.0
11	71056.0
12	71872.0
13	70450.0
14	68630.0
15	71796.0
16	76520.0
17	81974.0
18	85134.0
19	86075.0
20	89493.0
21	94933.0
22	102980.0
23	114878.0
24	129877.0
25	149048.0
26	170832.0
27	197150.0
28	227176.0
29	261462.0
30	303613.0
31	353795.0
32	417446.0
33	502530.0
34	614582.0
35	761467.0
36	927169.0
37	922547.0
38	437951.0
39	29060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53629821202543	17.33275976257082	12.80970267580388	25.32123934959987
2	16.520500191064734	19.332304944005138	38.39932295097724	25.747871913952885
3	20.292976705450865	22.802038822490342	27.935161834612042	28.969822637446747
4	14.18712666720738	14.97330264704407	34.870636568599004	35.96893411714955
5	15.49622782305583	36.19333779557099	32.861391732937065	15.449042648436109
6	34.72806600514758	33.16326578406874	16.941196308170785	15.1674719026129
7	30.634084851800132	30.655616800165138	19.685013002884862	19.02528534514987
8	27.59735216485959	33.39178288978587	19.506052865165653	19.50481208018889
9	27.620799555590462	15.79619103289518	18.701977217456463	37.881032194057894
10-11	26.61038529821295	24.60865617465295	26.51169861987289	22.26925990726121
12-13	27.088003382098474	21.80511312988307	26.571407035364093	24.53547645265437
14-15	24.57525647252548	23.39195175107597	24.017255971200562	28.015535805197988
16-17	24.633115886712396	26.29038265127569	24.702728534900427	24.37377292711149
18-19	24.57636709833792	25.82750390861325	25.968754396695893	23.627374596352947
20-21	25.263985415895363	25.014158813026345	25.812875205616194	23.908980565462105
22-23	25.210215125319497	24.619134831049955	25.486350574039307	24.68429946959124
24-25	24.817492042858053	24.88861524001912	25.535617295353013	24.758275421769813
26-27	24.529794146910668	25.13057311951191	25.67320179973046	24.666430933846957
28-29	24.607780389004805	25.299929025267648	25.485148764269276	24.607141821458274
30-31	25.11663844197351	25.07995629770275	25.249732014767506	24.553673245556237
32-33	24.845339445478412	25.11315844324325	25.26733171890384	24.774170392374497
34-35	25.04930991947128	25.101865283884983	25.38127005724607	24.46755473939767
36-37	25.07418255488204	24.88976278685552	25.441482222899424	24.59457243536301
38-39	25.003068461323373	25.103456647385936	25.31489111207325	24.578583779217436
40-41	25.03475649245639	24.929912709692704	25.395802171268034	24.63952862658287
42-43	24.83862981377076	24.812503505876805	25.28602521529388	25.06284146505856
44-45	24.611253440998347	24.946657368324463	25.469122407781242	24.972966782895945
46-47	24.81509360738384	24.753603499450787	25.36370635354909	25.067596539616275
48-49	24.688468159964895	24.880687809497008	25.396466240352318	25.03437779018578
50-51	24.65708294155774	24.980727606482947	25.48725079857035	24.874938653388963
52-53	24.642098024506126	24.95273136893246	25.483526661740623	24.921643944820794
54-55	24.2796942041617	24.798855321759813	25.787782876777193	25.13366759730129
56-57	24.499567573942976	24.809190974430592	25.588938210181382	25.102303241445046
58-59	24.494402199962487	24.739794466432873	25.736438123647353	25.029365209957287
60-61	24.72533406879456	24.701683336605594	25.704763334986986	24.868219259612857
62-63	24.498422370188745	24.750202984943144	25.77991293304066	24.971461711827452
64-65	24.355878683243187	24.557503891087496	25.832897139541856	25.25372028612746
66-67	24.3616941209262	24.69989128831194	25.63438407980988	25.304030510951986
68-69	24.23235276585336	24.63619633901506	25.70587321541153	25.425577679720053
70-71	24.267072787379742	24.475973115619816	25.811021177373522	25.44593291962692
72-73	24.229088156238383	24.670270876974154	25.661283910693417	25.439357056094046
74-75	24.04253599530774	24.790547916589997	25.80051250690465	25.36640358119761
76-77	24.099857538059748	24.494531876262666	25.818404843013354	25.58720574266423
78-79	24.06016520554248	24.467182804601205	25.92736974107264	25.54528224878368
80-81	24.18885124572171	24.61066849526335	25.75207015735256	25.44841010166238
82-83	24.181392050432386	24.54493995821368	25.807129126748336	25.4665388646056
84-85	24.196234859326243	24.56196884270463	25.68613567680127	25.55566062116786
86-87	23.997541949871152	24.6363943433153	25.89457793935225	25.4714857674613
88-89	23.918558317676432	24.391749687923596	25.973427992350835	25.716264002049137
90-91	24.08639425032267	24.251591114517105	26.018564807632583	25.643449827527643
92-93	24.301267849315014	24.206799655768606	25.924206294917656	25.56772619999872
94-95	24.220329731255624	24.22374023010947	25.966939528098486	25.58899051053642
96-97	24.11023324296112	24.07969762103321	26.040839427886187	25.76922970811949
98	24.081343660659613	24.000749473114766	25.870663762693148	26.04724310353248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	259.0
1	249.0
2	344.0
3	505.0
4	551.0
5	631.0
6	962.0
7	1659.5
8	2327.0
9	2609.5
10	2621.5
11	2702.5
12	3050.5
13	3503.0
14	3912.5
15	4149.5
16	4280.5
17	4292.0
18	4227.5
19	4181.0
20	4148.5
21	4114.0
22	4164.0
23	4429.5
24	4750.0
25	5305.0
26	6266.5
27	7356.5
28	8637.5
29	10711.0
30	14921.5
31	22506.5
32	34908.5
33	52063.5
34	72767.0
35	97713.5
36	126182.5
37	152222.0
38	175361.0
39	196814.0
40	216944.5
41	237012.0
42	259028.5
43	284675.5
44	307541.0
45	329050.0
46	353150.5
47	376561.5
48	395132.5
49	405630.5
50	408674.0
51	410142.0
52	412345.5
53	412400.5
54	406131.0
55	391925.0
56	367298.5
57	337179.0
58	305438.5
59	269869.5
60	232782.5
61	193335.5
62	154726.0
63	118178.0
64	86349.0
65	62581.0
66	45557.5
67	33781.5
68	24727.0
69	17724.0
70	12748.0
71	9268.0
72	6911.5
73	5109.0
74	3740.5
75	2718.0
76	1719.0
77	967.0
78	593.0
79	357.0
80	220.0
81	139.0
82	86.5
83	59.5
84	45.0
85	39.0
86	31.5
87	25.0
88	25.0
89	27.5
90	20.5
91	13.0
92	14.0
93	15.0
94	12.5
95	15.0
96	11.5
97	8.5
98	7.5
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31569299903316533
2	0.15492059865804142
3	0.15193617978232937
4	0.14565134473818278
5	0.14086457093360932
6	0.05192888843738988
7	0.041898900294310516
8	0.016361637600962597
9	0.010603464711353443
10-11	0.0019193909632030514
12-13	0.006659350354039855
14-15	0.011885009287394506
16-17	0.00785311790432468
18-19	0.05364346634049505
20-21	0.10599953630323194
22-23	0.10618679395817858
24-25	0.0828381051070195
26-27	0.09218343244920021
28-29	0.11293392133797467
30-31	0.0880813194455254
32-33	0.023881202807413578
34-35	0.33467039200166376
36-37	0.4348415337946816
38-39	0.40273854957476707
40-41	0.3990519144930052
42-43	0.3731635436966323
44-45	0.35478888630499333
46-47	0.3606172808152075
48-49	0.35296897597098065
50-51	0.35409837370237757
52-53	0.40383283649586155
54-55	0.39018643489162524
56-57	0.3738013900837943
58-59	0.3516932831966567
60-61	0.3616237907105457
62-63	0.36064068802207583
64-65	0.3779854283115083
66-67	0.4008776766287349
68-69	0.35911921957563436
70-71	0.33184397177231295
72-73	0.3151897440854962
74-75	0.300343723129258
76-77	0.30384310055607333
78-79	0.3108886698234406
80-81	0.30008624385370636
82-83	0.31035030406546904
84-85	0.3030121447122476
86-87	0.2976167835290976
88-89	0.32339982189456296
90-91	0.30494323927888484
92-93	0.2938482232232964
94-95	0.31086526261657227
96-97	0.30963638425598494
98	0.3088932054379155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	8544377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.144376827166234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31785188223643	35.03668814121679
2	11.875860066378646	11.197600442321006
3	4.958139012426601	7.012451218899406
4	2.602504868888636	4.907738809336828
5	1.515803645083433	3.573080911990275
6	0.987140698912545	2.7922879854579157
7	0.6875618680389883	2.269027305917428
8	0.4765882809385562	1.7974766006382925
9	0.36219437863808895	1.5367885444075853
>10	1.9682548535444182	17.04558079641695
>50	0.16469080035625094	5.3729597989026745
>100	0.08103042968850485	6.58101168733672
>500	0.0022290001205272653	0.6929335989709384
>1k	1.251789576788542E-4	0.12270365278413363
>5k	2.5035790767914098E-5	0.061670505403221006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
3	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
4	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
5	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
6	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
7	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
8	0.0	1.1703603434164949E-5	0.0	1.1703603434164949E-5	0.0
9	0.0	2.3407206868329898E-5	0.0	1.1703603434164949E-5	0.0
10-11	0.0	2.3407206868329898E-5	0.0	1.1703603434164949E-5	0.0
12-13	0.0	2.3407206868329898E-5	0.0	2.3407206868329898E-5	1.1703603434164949E-5
14-15	0.0	2.3407206868329898E-5	0.0	3.511081030249484E-5	1.1703603434164949E-5
16-17	0.0	2.3407206868329898E-5	0.0	3.511081030249484E-5	3.511081030249484E-5
18-19	0.0	2.3407206868329898E-5	0.0	4.0962612019577316E-5	3.511081030249484E-5
20-21	0.0	2.3407206868329898E-5	0.0	8.192522403915465E-5	7.02216206049897E-5
22-23	0.0	2.3407206868329898E-5	0.0	3.1599729272245363E-4	8.192522403915465E-5
24-25	0.0	2.3407206868329898E-5	0.0	7.373270163523917E-4	8.192522403915465E-5
26-27	0.0	2.3407206868329898E-5	0.0	0.0018667247477493092	9.948062919040207E-5
28-29	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.005576767036379598	1.0533243090748454E-4
30-31	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.021376631672502276	1.0533243090748454E-4
32-33	1.1703603434164949E-5	3.511081030249484E-5	0.0	0.05853557257597598	1.0533243090748454E-4
34-35	2.3407206868329898E-5	3.511081030249484E-5	0.0	0.10954572814378391	1.4044324120997937E-4
36-37	2.3407206868329898E-5	3.511081030249484E-5	0.0	0.19053466390820537	1.4044324120997937E-4
38-39	2.3407206868329898E-5	4.0962612019577316E-5	0.0	0.319215783666849	1.4044324120997937E-4
40-41	2.3407206868329898E-5	4.6814413736659796E-5	0.0	0.46980604905424933	1.4044324120997937E-4
42-43	2.3407206868329898E-5	4.6814413736659796E-5	0.0	0.6291272026035368	1.4629504292706187E-4
44-45	3.511081030249484E-5	4.6814413736659796E-5	5.8518017170824746E-6	0.82014756605426	1.5214684464414434E-4
46-47	3.511081030249484E-5	4.6814413736659796E-5	1.1703603434164949E-5	1.0420654425711786	1.638504480783093E-4
48-49	3.511081030249484E-5	4.6814413736659796E-5	1.1703603434164949E-5	1.2795959260692733	1.638504480783093E-4
50-51	3.511081030249484E-5	4.6814413736659796E-5	1.1703603434164949E-5	1.5327858309622808	1.7555405151247424E-4
52-53	4.0962612019577316E-5	4.6814413736659796E-5	1.1703603434164949E-5	1.7962632032739192	1.9896125838080413E-4
54-55	5.266621545374227E-5	4.6814413736659796E-5	1.1703603434164949E-5	2.0949450147155257	1.9896125838080413E-4
56-57	5.8518017170824744E-5	4.6814413736659796E-5	1.1703603434164949E-5	2.4353443205982135	1.9896125838080413E-4
58-59	5.8518017170824744E-5	5.266621545374227E-5	1.1703603434164949E-5	2.8044057512911706	1.9896125838080413E-4
60-61	5.8518017170824744E-5	5.8518017170824744E-5	1.1703603434164949E-5	3.1989576302637395	1.9896125838080413E-4
62-63	5.8518017170824744E-5	5.8518017170824744E-5	1.1703603434164949E-5	3.609613667561719	2.1066486181496908E-4
64-65	6.436981888790721E-5	5.8518017170824744E-5	1.1703603434164949E-5	4.055456588584516	2.1066486181496908E-4
66-67	8.192522403915465E-5	5.8518017170824744E-5	1.1703603434164949E-5	4.5575821385222115	2.1066486181496908E-4
68-69	8.192522403915465E-5	5.8518017170824744E-5	1.1703603434164949E-5	5.108745786849059	2.1066486181496908E-4
70-71	8.192522403915465E-5	5.8518017170824744E-5	1.1703603434164949E-5	5.706665330895395	2.1651666353205155E-4
72-73	8.192522403915465E-5	5.8518017170824744E-5	1.1703603434164949E-5	6.3194660067082715	2.282202669662165E-4
74-75	8.192522403915465E-5	5.8518017170824744E-5	1.1703603434164949E-5	6.968934072080387	2.457756721174639E-4
76-77	8.777702575623712E-5	5.8518017170824744E-5	1.1703603434164949E-5	7.668844668253753	2.5747927555162884E-4
78-79	9.362882747331959E-5	5.8518017170824744E-5	1.1703603434164949E-5	8.434611440951166	2.5747927555162884E-4
80-81	9.362882747331959E-5	5.8518017170824744E-5	1.1703603434164949E-5	9.244132135087204	2.633310772687113E-4
82-83	9.362882747331959E-5	5.8518017170824744E-5	1.1703603434164949E-5	10.07233178030417	2.691828789857938E-4
84-85	9.362882747331959E-5	5.8518017170824744E-5	1.1703603434164949E-5	10.919994518032151	2.691828789857938E-4
86	9.362882747331959E-5	5.8518017170824744E-5	1.1703603434164949E-5	11.585888590824117	2.691828789857938E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	15955	0.0	41.30427	1
GGTATCA	8955	0.0	35.92199	1
TCAACGC	20500	0.0	31.687645	4
TATCAAC	20695	0.0	31.390165	2
CAACGCA	21235	0.0	30.589773	5
ATCAACG	21530	0.0	30.172583	3
AACGCAG	22600	0.0	29.033049	6
GTACATG	32310	0.0	25.253439	1
ACGCAGA	26280	0.0	24.211096	7
TACATGG	33270	0.0	24.082598	2
CGCAGAG	27510	0.0	22.958881	8
ACATGGG	35190	0.0	22.324614	3
GCAGAGT	29580	0.0	19.861177	9
GTATATA	1745	0.0	19.779469	1
CATGGGG	26110	0.0	19.618345	4
AGTACAT	23990	0.0	19.131317	2
GAGTACA	25875	0.0	19.048372	1
GTATAGG	2015	0.0	17.585894	1
AGAGTAC	24210	0.0	17.334126	10-11
ATGGGGG	13945	0.0	15.84549	5
>>END_MODULE