##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8544377
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.85691654289131	31.0	30.0	34.0	26.0	34.0
2	29.829045464637154	31.0	30.0	34.0	26.0	34.0
3	30.017434858035877	31.0	30.0	34.0	26.0	34.0
4	33.65391473246089	35.0	35.0	37.0	28.0	37.0
5	33.11490890441749	35.0	33.0	37.0	27.0	37.0
6	32.989294128758594	35.0	33.0	37.0	26.0	37.0
7	32.791801672608784	35.0	33.0	37.0	26.0	37.0
8	32.68097697468171	35.0	33.0	37.0	25.0	37.0
9	33.97114230797634	37.0	33.0	39.0	25.0	39.0
10-11	33.754340778736704	37.0	33.0	39.0	25.0	39.0
12-13	33.570477578412095	37.0	33.0	39.0	23.0	39.0
14-15	34.413476313135526	38.0	33.0	40.0	23.0	41.0
16-17	34.26559987931245	38.0	33.0	40.0	22.0	41.0
18-19	34.135961755900986	38.0	33.0	40.0	20.0	41.0
20-21	33.99180256208264	38.0	33.0	40.0	18.5	41.0
22-23	33.81729329124873	38.0	32.5	40.0	18.0	41.0
24-25	33.66035861947571	38.0	32.0	40.0	18.0	41.0
26-27	33.567006289633525	38.0	32.0	40.0	18.0	41.0
28-29	33.57130765648566	38.0	32.0	40.0	17.5	41.0
30-31	33.41357216564765	38.0	32.0	40.0	16.5	41.0
32-33	33.24074645816775	38.0	32.0	40.0	16.0	41.0
34-35	33.03594568685347	38.0	31.5	40.0	15.0	41.0
36-37	32.862581321025516	38.0	31.0	40.0	14.5	41.0
38-39	32.68311949484439	37.5	31.0	40.0	9.0	41.0
40-41	32.69287280980228	38.0	31.0	40.0	9.0	41.0
42-43	32.49178916145671	37.0	31.0	40.0	9.0	41.0
44-45	32.286391915993406	37.0	31.0	40.0	8.0	41.0
46-47	32.01486977927121	37.0	30.0	40.0	6.5	41.0
48-49	31.72513045714158	37.0	30.0	40.0	2.0	41.0
50-51	31.456889250088096	36.0	30.0	40.0	2.0	41.0
52-53	31.136445933975057	36.0	29.0	39.0	2.0	41.0
54-55	30.82021667583254	35.5	29.0	39.0	2.0	40.5
56-57	30.47855074746819	35.0	28.0	39.0	2.0	40.0
58-59	30.007906895962105	35.0	28.0	39.0	2.0	40.0
60-61	29.657027715420327	34.5	28.0	38.0	2.0	40.0
62-63	29.323330712116285	34.0	27.0	38.0	2.0	40.0
64-65	28.88211855586428	34.0	26.0	37.5	2.0	40.0
66-67	28.469792297320215	34.0	26.0	37.0	2.0	39.0
68-69	28.051713659170233	33.5	26.0	36.0	2.0	39.0
70-71	27.60276313884558	33.0	25.5	36.0	2.0	39.0
72-73	27.147061628952	33.0	24.5	35.0	2.0	38.0
74-75	26.635348077454914	32.0	23.5	35.0	2.0	37.0
76-77	25.14578107918225	30.5	21.0	34.0	2.0	35.5
78-79	26.028900117586105	32.0	23.0	35.0	2.0	36.0
80-81	26.101550469975752	32.0	23.0	35.0	2.0	36.0
82-83	25.806050692753843	32.0	22.0	35.0	2.0	35.5
84-85	25.41331117529107	32.0	20.0	35.0	2.0	35.0
86-87	25.004380717283425	32.0	18.5	34.0	2.0	35.0
88-89	24.589470888281262	31.0	17.0	34.0	2.0	35.0
90-91	24.196371602048927	31.0	11.0	34.0	2.0	35.0
92-93	23.813338994756435	31.0	6.5	34.0	2.0	35.0
94-95	23.45800249684676	31.0	2.0	34.0	2.0	35.0
96-97	22.963263910288603	31.0	2.0	34.0	2.0	35.0
98-99	22.24619811368342	31.0	2.0	34.0	2.0	35.0
100-101	19.706033687418053	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	271678.0
3	119555.0
4	60895.0
5	42784.0
6	36663.0
7	36273.0
8	40628.0
9	45794.0
10	51606.0
11	57171.0
12	62838.0
13	67226.0
14	71569.0
15	75528.0
16	78934.0
17	80984.0
18	79315.0
19	76914.0
20	77633.0
21	80760.0
22	86560.0
23	98801.0
24	113517.0
25	134193.0
26	159482.0
27	187806.0
28	221244.0
29	258670.0
30	303886.0
31	357908.0
32	427053.0
33	518149.0
34	648139.0
35	821400.0
36	1054358.0
37	1113823.0
38	501887.0
39	22753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7217616167551	18.629513918260496	13.423753757724432	25.224970707259963
2	16.317234129533375	20.356533893577026	37.46435813869168	25.86187383819792
3	19.976091856519773	23.630896558891592	28.203896624782942	28.189114959805693
4	14.590666268059357	15.824893557099678	34.57154435438843	35.012895820452535
5	15.394319351583032	36.490345547845706	33.01342237727759	15.101912723293665
6	34.66816038263623	33.73900875105576	16.74065145589857	14.852179410409436
7	30.50827434942887	30.81299636281386	19.845652516584735	18.83307677117253
8	27.629162618347763	33.66110211093971	19.454682212680126	19.25505305803241
9	27.40005891052092	15.744727168599615	18.79499865143179	38.06021526944768
10-11	26.54937396507689	24.793481153978046	26.636787489695074	22.020357391249988
12-13	26.9702132375406	21.915357498317753	26.747485534560546	24.3669437295811
14-15	24.48126114225985	23.482922861888618	24.06804291634226	27.967773079509268
16-17	24.605382659868884	26.44253137657672	24.768292152311446	24.18379381124295
18-19	24.58170844477448	25.915265880473402	26.01293781217918	23.490087862572935
20-21	25.201629075102915	25.024617043811837	25.864904567604803	23.90884931348044
22-23	25.113682104280127	24.71052022348193	25.562325611549547	24.613472060688395
24-25	24.81491306664793	24.925247272535618	25.601031839450002	24.658807821366455
26-27	24.529165079047623	25.144566033750188	25.75729135758349	24.568977529618707
28-29	24.5931119749477	25.308278300429087	25.59848868657091	24.500121038052303
30-31	25.028344775731053	25.087671390844733	25.351231415067087	24.532752418357124
32-33	24.81990275038299	25.117777340280757	25.310623374131197	24.751696535205056
34-35	25.01388948896165	25.119010527442292	25.468656824068386	24.398443159527673
36-37	25.01726765845283	24.946838910645056	25.468887393792706	24.56700603710941
38-39	24.977116380620448	25.089274800598147	25.383173298252533	24.550435520528872
40-41	24.996758860193413	24.915044092085477	25.50039833096079	24.58779871676032
42-43	24.830916002360894	24.8671851864383	25.34356651932847	24.95833229187234
44-45	24.616593447884732	24.942185841091245	25.532805643055216	24.908415067968804
46-47	24.763620641978754	24.798390920485172	25.45409164227869	24.983896795257383
48-49	24.585763937613457	24.90672483536768	25.49379747512477	25.01371375189409
50-51	24.552623856385463	25.00322779676738	25.52606063997114	24.918087706876022
52-53	24.65624433974714	24.93032420757923	25.42457858457321	24.988852868100416
54-55	24.319328885912547	24.799837385983192	25.641703214436173	25.23913051366809
56-57	24.450820110585678	24.8545050044941	25.47412213444708	25.22055275047314
58-59	24.446930880434774	24.754462007507765	25.611018333066472	25.187588778990992
60-61	24.684742292614317	24.796060335233726	25.533099688564697	24.986097683587257
62-63	24.446686633726383	24.811249833567192	25.54191546237594	25.20014807033048
64-65	24.2789256289039	24.591152840510876	25.60752416778964	25.52239736279558
66-67	24.266645119491674	24.741274345599706	25.423131185115118	25.568949349793503
68-69	24.149500468855816	24.670793644175095	25.4457585473364	25.73394733963269
70-71	24.087881365308146	24.45478934357664	25.58297327815967	25.874356012955545
72-73	24.01107208153189	24.610977675150945	25.384713028373323	25.993237214943843
74-75	23.733644951461397	24.654635763624928	25.519791741983	26.091927542930677
76-77	23.686224741159347	24.319977299536326	25.759788695187858	26.23400926411647
78-79	23.546209352178348	24.284707211994004	25.83003117260035	26.339052263227302
80-81	23.538506928741615	24.457633537814548	25.65918430425185	26.34467522919199
82-83	23.39917168370179	24.329062569442613	25.791441667972244	26.480324078883356
84-85	23.351457317210222	24.251879359495927	25.65864210364643	26.73802121964742
86-87	23.034976621792435	24.34217244803289	25.89475767481599	26.728093255358687
88-89	22.926167179257927	24.129516650575678	25.913304863982432	27.03101130618396
90-91	22.942191311532454	23.947683292442264	25.980496690082454	27.129628705942828
92-93	22.978423976199988	23.826214648566086	25.9548985361715	27.24046283906243
94-95	22.678893827650608	23.873007684041177	26.016472925148538	27.431625563159674
96-97	22.462243546411433	23.6951098451223	26.176104846158427	27.666541762307844
98-99	22.21912062120504	23.60629704594741	26.192283228373398	27.98229910447415
100-101	22.37235474575936	23.442488371573138	26.28922183729671	27.89593504537079
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	199.0
2	237.0
3	311.0
4	298.0
5	332.0
6	552.0
7	1138.0
8	1669.5
9	1686.0
10	1397.5
11	1169.5
12	1188.0
13	1285.0
14	1380.5
15	1461.0
16	1568.5
17	1741.5
18	1916.0
19	2093.0
20	2307.0
21	2517.0
22	2801.5
23	3230.5
24	3643.5
25	4169.0
26	4913.0
27	5836.5
28	6952.5
29	8822.0
30	11913.0
31	17316.0
32	27355.0
33	41559.5
34	59064.0
35	79780.0
36	105185.0
37	131734.0
38	153328.5
39	173931.0
40	194872.0
41	214109.0
42	232948.5
43	256357.5
44	281332.5
45	302687.0
46	325886.5
47	349533.0
48	371459.5
49	390107.5
50	401099.5
51	407252.0
52	409414.5
53	410105.0
54	407510.5
55	393933.5
56	369023.0
57	338395.0
58	308189.5
59	275027.0
60	237462.0
61	197319.5
62	158446.5
63	122214.5
64	88850.5
65	63247.0
66	45969.5
67	34283.5
68	25137.0
69	18174.5
70	13176.0
71	9526.0
72	7014.5
73	5351.0
74	3949.5
75	2690.0
76	1795.0
77	1061.5
78	573.0
79	337.5
80	196.5
81	112.0
82	64.5
83	35.0
84	17.5
85	8.5
86	6.5
87	6.0
88	3.5
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24500323429080903
2	0.0
3	9.362882747331959E-5
4	0.015202980860980268
5	0.017309629479129958
6	0.1060814615272711
7	0.13672149531791494
8	0.1528256536433259
9	0.11000216867771634
10-11	0.11971030772635619
12-13	0.09560673645369346
14-15	0.11873890864132047
16-17	0.07256819309353976
18-19	0.07738422590669863
20-21	0.0533918388666605
22-23	0.08032768217039112
24-25	0.07287833858454514
26-27	0.05073512088710505
28-29	0.018831097925571402
30-31	0.03135980540184498
32-33	0.0394001809611163
34-35	0.0438416984643819
36-37	0.036521094516311726
38-39	0.03900811024607177
40-41	0.02173944337896139
42-43	0.03148854503962079
44-45	0.01740325830660328
46-47	0.0304176653253947
48-49	3.979225167616082E-4
50-51	0.016361637600962597
52-53	0.008186670602198381
54-55	0.016946817772670843
56-57	0.01118864488306169
58-59	0.00806378276613965
60-61	0.03083899504902464
62-63	0.013336256113230957
64-65	0.014506616456647452
66-67	0.058219575283253534
68-69	0.07690437816589787
70-71	0.033495713028580086
72-73	0.05500693614057526
74-75	0.04692559796928436
76-77	0.22752975436360076
78-79	0.08088945513523103
80-81	0.016098306523693887
82-83	0.0105507984958997
84-85	0.024636085228917217
86-87	0.17883106047404043
88-89	0.3848671471307972
90-91	0.27821806083696915
92-93	0.27945279099927356
94-95	0.002048130600978866
96-97	0.005927875139404546
98-99	0.004710700382251391
100-101	0.005196399924769237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8544377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.596737425849966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92958220096743	30.922983709408186
2	13.268821300195476	11.569546363502948
3	5.646857640897411	7.385537095540771
4	3.015153516482809	5.258034246269169
5	1.7413484850265806	3.795855633430273
6	1.0940940170969284	2.8619357749540915
7	0.7769794821529015	2.371163932808505
8	0.5756271460335639	2.0076372432653344
9	0.4180135732879123	1.6401625195566958
>10	2.2433964291178503	18.077018840080978
>50	0.19033925885264075	5.733807485095296
>100	0.09664746033088446	7.357223992209809
>500	0.0029230520329366332	0.8172223959220002
>1k	2.1643752480177592E-4	0.20187076795594455
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3407206868329898E-5
2	0.0	0.0	0.0	0.0	2.3407206868329898E-5
3	0.0	0.0	0.0	0.0	2.3407206868329898E-5
4	0.0	0.0	0.0	0.0	2.3407206868329898E-5
5	0.0	0.0	0.0	0.0	2.3407206868329898E-5
6	0.0	0.0	0.0	0.0	2.3407206868329898E-5
7	0.0	0.0	0.0	0.0	2.3407206868329898E-5
8	0.0	0.0	0.0	0.0	2.3407206868329898E-5
9	0.0	0.0	0.0	0.0	2.3407206868329898E-5
10-11	0.0	0.0	0.0	0.0	2.3407206868329898E-5
12-13	0.0	0.0	0.0	1.1703603434164949E-5	7.607342232207216E-5
14-15	0.0	0.0	0.0	3.511081030249484E-5	1.0533243090748454E-4
16-17	0.0	0.0	0.0	3.511081030249484E-5	1.1703603434164949E-4
18-19	5.8518017170824746E-6	0.0	0.0	4.0962612019577316E-5	1.1703603434164949E-4
20-21	1.1703603434164949E-5	5.8518017170824746E-6	0.0	9.948062919040205E-5	1.2873963777581442E-4
22-23	1.1703603434164949E-5	1.1703603434164949E-5	0.0	3.3940449959078353E-4	1.2873963777581442E-4
24-25	1.1703603434164949E-5	1.1703603434164949E-5	0.0	7.958450335232164E-4	1.2873963777581442E-4
26-27	1.1703603434164949E-5	1.1703603434164949E-5	0.0	0.0019427981700713814	1.4629504292706187E-4
28-29	1.1703603434164949E-5	1.1703603434164949E-5	0.0	0.005594322441530845	1.5214684464414434E-4
30-31	1.1703603434164949E-5	1.1703603434164949E-5	0.0	0.021253743836443545	1.5214684464414434E-4
32-33	1.1703603434164949E-5	1.1703603434164949E-5	0.0	0.05824883429183895	1.638504480783093E-4
34-35	1.1703603434164949E-5	1.1703603434164949E-5	0.0	0.10886106734288527	1.7555405151247424E-4
36-37	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.18953985761630135	1.7555405151247424E-4
38-39	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.31892904538271194	1.814058532295567E-4
40-41	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.47068381931181175	1.8725765494663919E-4
42-43	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.6305082278087683	1.8725765494663919E-4
44-45	1.1703603434164949E-5	2.3407206868329898E-5	0.0	0.8216924417075697	1.8725765494663919E-4
46-47	1.1703603434164949E-5	2.3407206868329898E-5	0.0	1.042796917785814	1.8725765494663919E-4
48-49	1.7555405151247424E-5	2.3407206868329898E-5	0.0	1.278571860768784	1.8725765494663919E-4
50-51	2.3407206868329898E-5	2.3407206868329898E-5	0.0	1.5274314323911504	1.8725765494663919E-4
52-53	2.3407206868329898E-5	2.3407206868329898E-5	0.0	1.7866252858458842	2.1066486181496908E-4
54-55	2.3407206868329898E-5	2.3407206868329898E-5	0.0	2.0804032874485756	2.3407206868329898E-4
56-57	2.3407206868329898E-5	2.925900858541237E-5	0.0	2.414459240269946	2.3407206868329898E-4
58-59	2.3407206868329898E-5	3.511081030249484E-5	0.0	2.776258585032004	2.3407206868329898E-4
60-61	2.3407206868329898E-5	3.511081030249484E-5	0.0	3.1643266677020456	2.5747927555162884E-4
62-63	2.3407206868329898E-5	3.511081030249484E-5	0.0	3.5677849888880138	2.8088648241995874E-4
64-65	2.3407206868329898E-5	3.511081030249484E-5	0.0	4.007021225772224	2.8088648241995874E-4
66-67	2.3407206868329898E-5	3.511081030249484E-5	0.0	4.499438636661281	2.8088648241995874E-4
68-69	2.3407206868329898E-5	3.511081030249484E-5	0.0	5.040039782888794	2.8088648241995874E-4
70-71	2.3407206868329898E-5	3.511081030249484E-5	0.0	5.628099041041846	2.867382841370412E-4
72-73	2.3407206868329898E-5	3.511081030249484E-5	0.0	6.233678593535842	3.0429368928828863E-4
74-75	2.3407206868329898E-5	3.511081030249484E-5	0.0	6.878096554026116	3.1599729272245363E-4
76-77	2.3407206868329898E-5	3.511081030249484E-5	0.0	7.574724289436199	3.1599729272245363E-4
78-79	2.3407206868329898E-5	3.511081030249484E-5	0.0	8.333568380702303	3.218490944395361E-4
80-81	2.3407206868329898E-5	3.511081030249484E-5	0.0	9.136862757811365	3.277008961566186E-4
82-83	2.3407206868329898E-5	3.511081030249484E-5	0.0	9.962598794505439	3.277008961566186E-4
84-85	2.3407206868329898E-5	3.511081030249484E-5	0.0	10.8095007980102	3.277008961566186E-4
86-87	2.3407206868329898E-5	3.511081030249484E-5	0.0	11.705792008007137	3.335526978737011E-4
88-89	2.3407206868329898E-5	4.0962612019577316E-5	0.0	12.65281833889118	3.3940449959078353E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	15225	0.0	39.83039	1
GGTATCA	8960	0.0	38.35585	1
TCAACGC	20085	0.0	29.413328	4
TATCAAC	20410	0.0	29.036669	2
CAACGCA	20680	0.0	28.589684	5
ATCAACG	21250	0.0	28.046612	3
AACGCAG	22210	0.0	26.487562	6
GTACATG	32395	0.0	25.272774	1
TACATGG	33230	0.0	24.179384	2
ACATGGG	35415	0.0	22.340012	3
ACGCAGA	26635	0.0	21.427475	7
CGCAGAG	27795	0.0	20.259897	8
CATGGGG	26865	0.0	20.116434	4
GTATAGG	2140	0.0	18.906347	1
GAGTACA	25930	0.0	18.57721	1
AGTACAT	24440	0.0	18.108812	2
GCAGAGT	30135	0.0	17.550518	9
GTATATA	1845	0.0	17.027466	1
AGAGTAC	24445	0.0	16.798637	10-11
ATGGGGG	14190	0.0	15.494912	5
>>END_MODULE