FastQCFastQC Report
Mon 15 Aug 2016
SRR2921563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316348
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115050.045.6402241
GGTATCA59150.042.947021
TCAACGC149400.034.8728334
TATCAAC151600.034.246622
CAACGCA152000.034.1543355
ATCAACG153550.033.7812543
AACGCAG161850.032.038136
ACGCAGA189300.026.8779667
GTACATG225100.025.3101021
CGCAGAG199100.025.1133928
TACATGG231250.024.1397672
ACATGGG241100.022.829233
GCAGAGT214850.021.8389579
CATGGGG184850.020.4055564
GTATATA12000.018.7915921
AGAGTAC182250.018.66153110-11
GAGTACA176650.017.3764441
CAGAGTA196400.017.28199810-11
AGTACAT169000.017.1546232
ATGGGGG105950.016.997935
Produced by FastQC (version 0.11.5)