##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921563_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6316348
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.150863125337615	31.0	28.0	31.0	16.0	34.0
2	28.12290725590167	31.0	28.0	33.0	16.0	34.0
3	28.215255872539004	31.0	28.0	34.0	16.0	34.0
4	31.63917298413577	35.0	32.0	37.0	18.0	37.0
5	31.577003831961125	35.0	32.0	37.0	17.0	37.0
6	31.67173705438649	35.0	32.0	37.0	18.0	37.0
7	31.479946956690796	35.0	32.0	37.0	17.0	37.0
8	31.432567838250836	35.0	32.0	37.0	17.0	37.0
9	32.67221850347701	37.0	32.0	39.0	17.0	39.0
10-11	32.444453187189815	37.0	32.0	39.0	17.0	39.0
12-13	32.254522075097825	37.0	32.0	39.0	15.0	39.0
14-15	32.96364837719518	38.0	32.0	40.0	11.0	41.0
16-17	32.82144896069691	38.0	32.0	40.0	10.5	41.0
18-19	32.646307090742944	38.0	31.0	40.0	10.0	41.0
20-21	32.51909695285947	38.0	31.0	40.0	10.0	41.0
22-23	32.29761398516991	37.0	31.0	40.0	9.5	41.0
24-25	32.39506143423384	38.0	31.0	40.0	8.5	41.0
26-27	32.151885234949056	37.0	30.5	40.0	8.0	41.0
28-29	31.958416714848518	37.0	30.0	40.0	5.0	41.0
30-31	31.705918594098996	37.0	30.0	40.0	2.0	41.0
32-33	31.461440534942028	37.0	30.0	40.0	2.0	41.0
34-35	31.119986105895368	36.5	29.5	40.0	2.0	41.0
36-37	30.815612518499613	36.0	29.0	40.0	2.0	41.0
38-39	30.593378721375075	36.0	28.5	39.0	2.0	41.0
40-41	30.366652771506573	36.0	28.0	39.0	2.0	40.0
42-43	30.112385828013274	35.5	27.5	39.0	2.0	40.0
44-45	29.84609769759361	35.0	27.0	39.0	2.0	40.0
46-47	29.544082751615335	35.0	26.0	39.0	2.0	40.0
48-49	29.251348247436653	35.0	26.0	39.0	2.0	40.0
50-51	28.31171073854702	34.0	25.0	38.0	2.0	39.5
52-53	28.511342709426394	34.0	25.0	38.0	2.0	39.5
54-55	29.08481522867328	35.0	26.0	39.0	2.0	40.0
56-57	29.063768335753508	35.0	26.0	39.0	2.0	40.0
58-59	28.822310376185733	34.5	26.0	39.0	2.0	40.0
60-61	28.47458982627303	34.0	26.0	38.0	2.0	40.0
62-63	28.051362432848855	34.0	24.5	38.0	2.0	40.0
64-65	27.586903381510965	34.0	23.0	37.5	2.0	40.0
66-67	27.23572735384434	33.0	22.5	37.0	2.0	39.5
68-69	26.77627847610676	33.0	21.5	36.0	2.0	39.0
70-71	26.349205981051078	33.0	20.5	36.0	2.0	39.0
72-73	25.87235353403581	32.0	19.0	35.0	2.0	38.5
74-75	25.420977042430216	32.0	18.0	35.0	2.0	37.0
76-77	24.98700419926198	32.0	14.0	35.0	2.0	37.0
78-79	24.58187230975874	31.0	8.5	35.0	2.0	36.0
80-81	24.13287369536954	31.0	6.0	34.5	2.0	36.0
82-83	23.58906744846864	31.0	2.0	34.0	2.0	35.0
84-85	23.164825386441656	30.5	2.0	34.0	2.0	35.0
86-87	22.66019343772699	30.0	2.0	34.0	2.0	35.0
88-89	22.136553828256453	30.0	2.0	34.0	2.0	35.0
90-91	21.536013373550666	29.0	2.0	34.0	2.0	35.0
92-93	20.98873510452559	29.0	2.0	34.0	2.0	35.0
94-95	20.304808173963814	29.0	2.0	34.0	2.0	35.0
96-97	19.65882262978544	27.0	2.0	34.0	2.0	35.0
98	19.177219652875365	27.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	387749.0
3	65317.0
4	47655.0
5	38787.0
6	35820.0
7	38028.0
8	42292.0
9	48936.0
10	51181.0
11	53979.0
12	53864.0
13	52602.0
14	52198.0
15	54261.0
16	58573.0
17	61584.0
18	64746.0
19	64668.0
20	66801.0
21	70918.0
22	77694.0
23	86359.0
24	96889.0
25	111835.0
26	127979.0
27	147459.0
28	169600.0
29	194141.0
30	225569.0
31	262386.0
32	308692.0
33	370855.0
34	455514.0
35	561684.0
36	681635.0
37	680523.0
38	325805.0
39	21769.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45159964528555	17.41200743985608	12.956335947107908	25.18005696775046
2	16.369833256166476	19.509900613317942	38.6351773040649	25.485088826450685
3	19.796060842118315	23.33411713333421	28.36379820706939	28.50602381747808
4	14.112380657028856	15.333025116207684	35.05121572947415	35.503378497289305
5	15.256037383622074	36.30815329187524	33.13103688818603	15.304772436316652
6	34.291030012313605	33.50730880728464	17.145340771971483	15.056320408430274
7	30.15616662651673	30.878770471122692	20.245340667071808	18.719722235288767
8	27.34867325355553	33.45570666123653	19.741221976719032	19.454398108488906
9	27.634919840726475	15.653811246808736	18.767460522040714	37.94380839042408
10-11	26.37358470521805	24.58916286870651	26.881618031165637	22.155634394909804
12-13	27.020614036962677	21.943253410142184	26.76544216149016	24.270690391404983
14-15	24.436308505921843	23.36604598137944	24.197075495598202	28.00057001710051
16-17	24.57830218889171	26.263613034947298	24.855453348253427	24.302631427907567
18-19	24.561091013031145	25.778937770119235	26.127761800778813	23.532209416070806
20-21	25.27303407423725	25.023611434122735	25.815683031899113	23.88767145974091
22-23	25.13610926395609	24.641236360547474	25.609880754433757	24.61277362106268
24-25	24.75413623110608	24.90343697505887	25.617016260360977	24.725410533474072
26-27	24.594554123625578	25.0869616199454	25.697261691409846	24.62122256501918
28-29	24.68055698195885	25.34616430644468	25.466489348888103	24.506789362708368
30-31	25.090651594207586	25.06537871644996	25.29961630384242	24.544353385500038
32-33	24.744643764145493	25.061463249327687	25.3102299661003	24.883663020426518
34-35	25.000921254939833	25.10995495381018	25.428661511874655	24.460462279375324
36-37	25.011582859339725	24.97366231091797	25.416521847800748	24.59823298194155
38-39	25.00529090521408	25.03800987895251	25.32927788660702	24.627421329226387
40-41	25.06326005543028	24.882807672822274	25.348962420654235	24.70496985109321
42-43	24.839640750789947	24.841118514850297	25.257395116688937	25.06184561767082
44-45	24.719198766340504	24.90620497268742	25.435195343321453	24.939400917650623
46-47	24.860894065639634	24.767084347241966	25.349770674997824	25.022250912120576
48-49	24.72819369626706	24.89700623840665	25.298567524063653	25.076232541262637
50-51	24.707652241066054	24.92809017628124	25.440528345668547	24.923729236984165
52-53	24.65719392470815	24.892905139329415	25.48842583946142	24.961475096501008
54-55	24.30094556533078	24.67452313494301	25.784011884427994	25.240519415298223
56-57	24.48241008645474	24.78603211548188	25.58060880443503	25.150948993628354
58-59	24.561464267779527	24.72501608908276	25.65455281722977	25.05896682590794
60-61	24.75219028532217	24.57863729243667	25.718548475121633	24.95062394711952
62-63	24.497224469031448	24.633788631945002	25.77603095973363	25.09295593928992
64-65	24.34348670224684	24.4904511634958	25.881008126784316	25.285054007473047
66-67	24.394754177258505	24.683409052058565	25.612521400569793	25.309315370113133
68-69	24.289805360181376	24.63311403055834	25.677180066060924	25.39990054319936
70-71	24.288343806279862	24.48005817188837	25.734881904665546	25.49671611716623
72-73	24.27626341890651	24.690152739007306	25.619100971477415	25.41448287060877
74-75	24.164181519460364	24.695395132451292	25.722762212339678	25.41766113574866
76-77	24.16918860762579	24.40913855017103	25.74327356921451	25.67839927298867
78-79	24.16229921264344	24.449318080076583	25.8703981342662	25.51798457301378
80-81	24.269786988835808	24.591694661370926	25.67841291721028	25.460105432582985
82-83	24.220480140991224	24.56186584697043	25.77005125906971	25.447602752968635
84-85	24.21232859038705	24.58288259927695	25.663985353144593	25.540803457191412
86-87	24.078002776796854	24.617205978369945	25.81389776662204	25.490893478211163
88-89	24.054061952214045	24.330467289957117	25.915491928760847	25.699978829067994
90-91	24.23380438963026	24.25106526771511	25.873603687063294	25.641526655591335
92-93	24.28362421402434	24.304733609197697	25.838987314277613	25.572654862500354
94-95	24.266244865913478	24.200746753123013	25.903435661463448	25.629572719500064
96-97	24.144006517166716	24.10741891455191	25.932082674642682	25.816491893638695
98	24.14305040185008	24.07033611026021	25.86529451193688	25.921318975952833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	322.0
1	284.0
2	362.5
3	506.5
4	519.5
5	594.5
6	850.5
7	1458.0
8	2117.5
9	2332.0
10	2224.0
11	2240.0
12	2486.0
13	2781.0
14	3069.5
15	3346.0
16	3521.0
17	3539.0
18	3510.0
19	3482.5
20	3416.5
21	3397.0
22	3532.0
23	3808.5
24	4170.5
25	4634.0
26	5394.0
27	6402.5
28	7864.0
29	10284.0
30	14367.0
31	20505.5
32	29302.0
33	40197.5
34	53133.5
35	68705.0
36	86040.0
37	104406.0
38	121712.5
39	137272.0
40	153189.5
41	169619.5
42	189014.5
43	210437.5
44	230291.5
45	250043.5
46	268802.0
47	285442.0
48	297712.0
49	304007.5
50	305925.0
51	306256.5
52	307359.5
53	307272.5
54	301834.0
55	288954.5
56	268181.5
57	243998.5
58	220111.0
59	194477.5
60	166755.0
61	138904.5
62	112809.0
63	87061.0
64	64592.0
65	47926.0
66	35538.0
67	26822.0
68	20031.0
69	14807.0
70	10661.5
71	7574.0
72	5521.0
73	3967.5
74	2831.5
75	1972.5
76	1318.0
77	832.5
78	512.0
79	312.5
80	192.5
81	109.0
82	66.5
83	50.0
84	40.0
85	30.0
86	21.5
87	17.0
88	19.0
89	19.5
90	14.5
91	15.5
92	13.5
93	11.0
94	11.5
95	13.5
96	10.5
97	4.5
98	3.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30796276582607546
2	0.15306313078380102
3	0.14988091219799796
4	0.1441655842901626
5	0.1388935505136829
6	0.047590791387681616
7	0.039817312155695034
8	0.015420302997871555
9	0.010639059152535612
10-11	0.0020344034242571816
12-13	0.006475260704445037
14-15	0.011842286080500948
16-17	0.0076072439327282155
18-19	0.05192082513503055
20-21	0.10093649051635532
22-23	0.10043778461858023
24-25	0.07798810325206908
26-27	0.08736060774358854
28-29	0.1072138520550166
30-31	0.08287225466361259
32-33	0.022093462868100367
34-35	0.3259478420125047
36-37	0.4255940299679498
38-39	0.39408056680854187
40-41	0.3904233902248578
42-43	0.3650289692714841
44-45	0.34679058215285163
46-47	0.3518488848302848
48-49	0.34500948966079764
50-51	0.34565068295793705
52-53	0.3952125500368251
54-55	0.3812408689324907
56-57	0.3640869692423533
58-59	0.343663775333468
60-61	0.3531312714245637
62-63	0.35205469996269995
64-65	0.369359003018833
66-67	0.39186409615176365
68-69	0.35080397723494655
70-71	0.32492668231706046
72-73	0.308651454923003
74-75	0.2944581267529908
76-77	0.297394950373222
78-79	0.30398895057713726
80-81	0.2941494040543681
82-83	0.3042660094092346
84-85	0.29685666464229016
86-87	0.29200417709727206
88-89	0.3163061946555193
90-91	0.29874858066718296
92-93	0.2882124290808549
94-95	0.30428975730912866
96-97	0.3029123791152736
98	0.30237409338434174
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6316348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.765695480836165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78897488441937	43.937219806517795
2	11.022890610157337	12.293983221692047
3	3.843851664646346	6.430651842125198
4	1.9169082248190514	4.275908813198778
5	1.0440996057100693	2.9112470331844413
6	0.6816001745170377	2.2805944663081146
7	0.4788718493142695	1.8693235206243088
8	0.3269551036192023	1.4586302995466938
9	0.25861317822573915	1.2979569369841002
>10	1.4909150751752278	15.42404810795945
>50	0.10747083049394686	4.079831054155073
>100	0.03816181745324878	3.3509484747756724
>500	6.011108075230174E-4	0.2323018424999069
>1k	8.587064150106553E-5	0.15735458042859465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.499159957621081E-5
2	0.0	0.0	0.0	0.0	9.499159957621081E-5
3	0.0	0.0	0.0	0.0	9.499159957621081E-5
4	0.0	0.0	0.0	1.58319332627018E-5	1.108235328389126E-4
5	0.0	3.16638665254036E-5	0.0	1.58319332627018E-5	1.266554661016144E-4
6	0.0	3.16638665254036E-5	0.0	1.58319332627018E-5	2.216470656778252E-4
7	0.0	3.16638665254036E-5	0.0	1.58319332627018E-5	2.216470656778252E-4
8	0.0	3.16638665254036E-5	0.0	1.58319332627018E-5	2.216470656778252E-4
9	0.0	3.16638665254036E-5	0.0	3.16638665254036E-5	2.3747899894052704E-4
10-11	0.0	3.16638665254036E-5	0.0	7.12436996821581E-5	2.3747899894052704E-4
12-13	0.0	6.332773305080722E-5	0.0	9.499159957621081E-5	3.5621849841079055E-4
14-15	0.0	8.707563294485991E-5	0.0	1.0290756620756171E-4	4.432941313556504E-4
16-17	0.0	9.499159957621081E-5	0.0	1.424873993643162E-4	4.907899311437559E-4
18-19	0.0	1.108235328389126E-4	0.0	2.0581513241512342E-4	6.016134639826684E-4
20-21	0.0	1.266554661016144E-4	0.0	2.3747899894052704E-4	6.807731302961775E-4
22-23	0.0	1.266554661016144E-4	0.0	4.3537816472429957E-4	7.599327966096865E-4
24-25	0.0	1.266554661016144E-4	0.0	9.103361626053536E-4	8.390924629231955E-4
26-27	0.0	1.4248739936431623E-4	0.0	0.0020185714909944797	8.945042293426518E-4
28-29	0.0	1.4248739936431623E-4	0.0	0.006435680871288282	9.182521292367045E-4
30-31	0.0	1.4248739936431623E-4	0.0	0.024586992356975895	9.578319623934591E-4
32-33	0.0	1.5831933262701802E-4	0.0	0.06590042220599625	0.0010765714618637225
34-35	0.0	1.7415126588971982E-4	0.0	0.12182672645649037	0.0011398991949145298
36-37	0.0	1.820672325210707E-4	0.0	0.20762788877370278	0.0011557311281772315
38-39	0.0	2.1373109904647433E-4	0.0	0.3445741114960733	0.0011715630614399333
40-41	0.0	2.453949655718779E-4	0.0	0.4990700322401489	0.0011794790280712842
42-43	0.0	2.8497479872863245E-4	0.0	0.6578089110986285	0.001203226927965337
44-45	0.0	2.9289076535998337E-4	0.0	0.8476812867182112	0.0012586386943847934
46-47	0.0	3.008067319913342E-4	0.0	1.0687663187652106	0.0013536302939610042
48-49	7.9159666313509E-6	3.008067319913342E-4	0.0	1.303166006686142	0.0014961176933253203
50-51	1.58319332627018E-5	3.0872269862268514E-4	0.0	1.5566510901552606	0.0015673613930074783
52-53	1.58319332627018E-5	3.245546318853869E-4	0.0	1.8148620057032956	0.0016702689592150402
54-55	1.58319332627018E-5	3.7996639830484324E-4	0.0	2.1013408380918848	0.0017969244253166544
56-57	1.58319332627018E-5	3.7996639830484324E-4	0.0	2.4282227641668888	0.0018048403919480053
58-59	1.58319332627018E-5	4.037142981988959E-4	0.0	2.7820031448552234	0.001820672325210707
60-61	1.58319332627018E-5	4.1163026483024683E-4	0.0	3.1628244675562525	0.0018681681249988126
62-63	1.58319332627018E-5	4.2746219809294866E-4	0.0	3.5616941941767615	0.0019473277913123217
64-65	1.58319332627018E-5	4.3537816472429957E-4	0.0	3.99167366965848	0.0019869076244690763
66-67	3.16638665254036E-5	4.432941313556504E-4	0.0	4.468175281032647	0.0020344034242571816
68-69	3.16638665254036E-5	4.432941313556504E-4	0.0	5.0007694319565665	0.0020581513241512343
70-71	3.16638665254036E-5	4.74957997881054E-4	0.0	5.578239197713615	0.002153142923727445
72-73	3.16638665254036E-5	5.066218644064576E-4	0.0	6.183019048348824	0.002414369822562025
74-75	3.16638665254036E-5	5.066218644064576E-4	0.0	6.809749874452769	0.0024460336890874284
76-77	3.16638665254036E-5	5.145378310378085E-4	0.0	7.484981828107001	0.002477697555612832
78-79	3.16638665254036E-5	5.382857309318613E-4	0.0	8.220573027325283	0.0025014454555068845
80-81	3.16638665254036E-5	5.382857309318613E-4	0.0	8.999678295116102	0.0025014454555068845
82-83	5.54117664194563E-5	5.382857309318613E-4	0.0	9.808769244506477	0.002564773188557692
84-85	6.33277330508072E-5	5.382857309318613E-4	0.0	10.640183219797262	0.002564773188557692
86	6.33277330508072E-5	5.382857309318613E-4	0.0	11.282310600999185	0.002564773188557692
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11505	0.0	45.640224	1
GGTATCA	5915	0.0	42.94702	1
TCAACGC	14940	0.0	34.872833	4
TATCAAC	15160	0.0	34.24662	2
CAACGCA	15200	0.0	34.154335	5
ATCAACG	15355	0.0	33.781254	3
AACGCAG	16185	0.0	32.03813	6
ACGCAGA	18930	0.0	26.877966	7
GTACATG	22510	0.0	25.310102	1
CGCAGAG	19910	0.0	25.113392	8
TACATGG	23125	0.0	24.139767	2
ACATGGG	24110	0.0	22.82923	3
GCAGAGT	21485	0.0	21.838957	9
CATGGGG	18485	0.0	20.405556	4
GTATATA	1200	0.0	18.791592	1
AGAGTAC	18225	0.0	18.661531	10-11
GAGTACA	17665	0.0	17.376444	1
CAGAGTA	19640	0.0	17.281998	10-11
AGTACAT	16900	0.0	17.154623	2
ATGGGGG	10595	0.0	16.99793	5
>>END_MODULE