FastQCFastQC Report
Mon 15 Aug 2016
SRR2921563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316348
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110850.044.2680241
GGTATCA59700.043.449961
TCAACGC147250.032.02654
TATCAAC149150.031.9028152
CAACGCA150400.031.4186365
ATCAACG154900.030.5367953
AACGCAG160850.029.4608866
GTACATG230350.025.4146481
TACATGG236450.024.401782
ACGCAGA192950.024.1166867
ACATGGG246000.022.645323
CGCAGAG205400.022.3771298
CATGGGG191150.020.298474
GCAGAGT220750.019.6144169
AGAGTAC188000.017.79010810-11
ATGGGGG105850.016.7800355
GTGGTAT37450.016.7760351
GTATAGG12100.016.52081
AGTACAT175850.016.4459692
GAGTACA185700.016.4034521
Produced by FastQC (version 0.11.5)