##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6316348
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.00167232711054	31.0	30.0	34.0	26.0	34.0
2	29.983792374960974	31.0	30.0	34.0	26.0	34.0
3	30.164194721380138	31.0	30.0	34.0	26.0	34.0
4	33.816124127422995	35.0	35.0	37.0	30.0	37.0
5	33.30869261794949	35.0	33.0	37.0	27.0	37.0
6	33.19338263186259	35.0	33.0	37.0	27.0	37.0
7	32.99830091692225	35.0	33.0	37.0	26.0	37.0
8	32.89325192342157	35.0	33.0	37.0	26.0	37.0
9	34.20247744424468	37.0	34.0	39.0	26.0	39.0
10-11	33.988950418817964	37.0	33.0	39.0	25.0	39.0
12-13	33.80850120987634	37.0	33.0	39.0	25.0	39.0
14-15	34.66322849849311	38.0	33.0	40.0	25.0	41.0
16-17	34.51822920459734	38.0	33.0	40.0	24.0	41.0
18-19	34.38760965988575	38.0	33.0	40.0	23.5	41.0
20-21	34.249017391062054	38.0	33.0	40.0	22.0	41.0
22-23	34.073750527994974	38.0	33.0	40.0	21.0	41.0
24-25	33.91477519921322	38.0	33.0	40.0	19.0	41.0
26-27	33.82216400996272	38.0	32.5	40.0	19.0	41.0
28-29	33.824638066173684	38.0	33.0	40.0	19.0	41.0
30-31	33.66644348918078	38.0	32.0	40.0	18.0	41.0
32-33	33.49218852412818	38.0	32.0	40.0	17.5	41.0
34-35	33.281098824827254	38.0	32.0	40.0	16.0	41.0
36-37	33.10885673176969	38.0	32.0	40.0	16.0	41.0
38-39	32.926734799919195	38.0	31.0	40.0	15.0	41.0
40-41	32.931633912507664	38.0	32.0	40.0	15.0	41.0
42-43	32.726975302817394	37.5	31.0	40.0	11.0	41.0
44-45	32.519476523459446	37.0	31.0	40.0	9.0	41.0
46-47	32.245663633479346	37.0	31.0	40.0	8.0	41.0
48-49	31.95443435035562	37.0	30.0	40.0	7.0	41.0
50-51	31.682940917758174	36.5	30.0	40.0	4.0	41.0
52-53	31.36034176710973	36.0	30.0	39.5	2.0	41.0
54-55	31.047244230368563	36.0	29.0	39.0	2.0	40.5
56-57	30.70163273144545	35.0	28.5	39.0	2.0	40.0
58-59	30.23134135421291	35.0	28.0	39.0	2.0	40.0
60-61	29.886989681379177	35.0	28.0	38.0	2.0	40.0
62-63	29.560941702388785	34.0	27.5	38.0	2.0	40.0
64-65	29.117133666479425	34.0	26.5	37.5	2.0	40.0
66-67	28.703652094533105	34.0	26.0	37.0	2.0	39.5
68-69	28.284158820888273	34.0	26.0	36.5	2.0	39.0
70-71	27.831284549236365	33.0	26.0	36.0	2.0	39.0
72-73	27.36913426872617	33.0	25.0	35.5	2.0	38.5
74-75	26.85417499162491	32.5	24.0	35.0	2.0	37.0
76-77	25.354122904564473	30.5	21.5	34.0	2.0	36.0
78-79	26.241384261918437	32.0	23.5	35.0	2.0	36.0
80-81	26.30933602771728	32.0	24.0	35.0	2.0	36.0
82-83	26.011111484041095	32.0	23.0	35.0	2.0	35.5
84-85	25.61401897108899	32.0	20.5	35.0	2.0	35.0
86-87	25.197126804919552	32.0	19.5	34.5	2.0	35.0
88-89	24.77694634621145	31.5	18.5	34.0	2.0	35.0
90-91	24.37992309796737	31.0	14.5	34.0	2.0	35.0
92-93	23.98846303275247	31.0	8.5	34.0	2.0	35.0
94-95	23.63006241898008	31.0	2.0	34.0	2.0	35.0
96-97	23.125955852970737	31.0	2.0	34.0	2.0	35.0
98-99	22.400312728177738	31.0	2.0	34.0	2.0	35.0
100-101	19.83653964284425	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	174203.0
3	79919.0
4	42143.0
5	30275.0
6	26111.0
7	25990.0
8	29171.0
9	33187.0
10	37673.0
11	41970.0
12	46054.0
13	50136.0
14	52908.0
15	56021.0
16	57998.0
17	59852.0
18	58661.0
19	57383.0
20	57412.0
21	59993.0
22	64565.0
23	72720.0
24	84297.0
25	99609.0
26	118264.0
27	139620.0
28	163898.0
29	192289.0
30	225170.0
31	265557.0
32	318224.0
33	386912.0
34	480724.0
35	613356.0
36	784170.0
37	832743.0
38	380005.0
39	17165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78598974527286	18.47304646527424	13.523340082105745	25.21762370734716
2	16.285375663278845	20.507103155177646	37.436806838381926	25.77071434316159
3	19.652840313953767	23.917490877634275	28.479940294575467	27.949728513836487
4	14.63193536404278	16.154365755077908	34.51025367136759	34.70344520951172
5	15.342331399475912	36.54113298741465	33.05705441513441	15.05948119797503
6	34.45800794792249	33.88076531441046	16.859342374788525	14.801884362878525
7	30.208949952139548	30.916187612973022	20.200661961260778	18.674200473626655
8	27.45718745713989	33.63766885396921	19.593632642577006	19.311511046313893
9	27.53141144347145	15.576566958565866	18.61269479405923	38.27932680390346
10-11	26.42213046544988	24.72979655885686	26.830011333106143	22.01806164258712
12-13	27.05329668745006	21.974004854776638	26.78307978181078	24.189618675962517
14-15	24.405387233351888	23.439649684961193	24.14474635223228	28.01021672945464
16-17	24.616858146495534	26.34011323387663	24.841164899526163	24.201863720101674
18-19	24.64826539968986	25.80414276066536	26.07848628222978	23.469105557415006
20-21	25.3009729422345	24.991068129716655	25.788224187370083	23.91973474067876
22-23	25.12041097660418	24.65360528649284	25.613890598536255	24.612093138366724
24-25	24.84581893525829	24.885497973113313	25.593177455208792	24.675505636419608
26-27	24.633882494082478	25.065570536330835	25.69788458049152	24.60266238909517
28-29	24.709270540842578	25.36888622159974	25.463618092401774	24.458225145155907
30-31	25.111106887969825	25.103616091118496	25.253004435090187	24.53227258582149
32-33	24.832260365615703	25.097593393171184	25.238970774564013	24.831175466649096
34-35	25.018222330487976	25.1264396908175	25.416215134053317	24.439122844641208
36-37	25.054106833587564	25.005588657246996	25.403513723231956	24.536790785933484
38-39	25.011434931911474	25.0485192713446	25.356161701974	24.583884094769928
40-41	25.034497196043837	24.908939120564572	25.395724089573314	24.660839593818277
42-43	24.84184652437684	24.8541516613677	25.299219122657984	25.004782691597477
44-45	24.72259328016701	24.926004817524998	25.4628459627101	24.88855593959789
46-47	24.800517857779152	24.80117507789642	25.420260591735655	24.978046472588776
48-49	24.652879641247086	24.956149343170278	25.371382884826225	25.019588130756414
50-51	24.673790781941758	24.928774285919907	25.44879622308357	24.948638709054766
52-53	24.692906845586236	24.87902630783953	25.430861409320954	24.997205437253278
54-55	24.366062366098788	24.71380570013412	25.654870956044775	25.26526097772232
56-57	24.466845365245106	24.78794387857617	25.453630125609934	25.291580630568795
58-59	24.52375521971056	24.768995621883285	25.497053560621	25.210195597785155
60-61	24.703881807693616	24.654772264589738	25.529044074270857	25.112301853445786
62-63	24.424959619274	24.69019539663682	25.569910156150417	25.314934827938764
64-65	24.27130529897813	24.5254992377032	25.65977249298091	25.543422970337758
66-67	24.335112218005538	24.73359896476294	25.38761590221198	25.54367291501954
68-69	24.226241624620272	24.648410116675233	25.427850508735574	25.697497749968928
70-71	24.138923308095396	24.486839721369133	25.524380834372245	25.849856136163226
72-73	24.083507188499603	24.619388463314113	25.378611684160163	25.91849266402612
74-75	23.89863523983922	24.58123707019511	25.457806762962115	26.062320927003558
76-77	23.837179089783735	24.29711010330518	25.667946024669448	26.197764782241645
78-79	23.782783389366475	24.331513923173766	25.72824006232002	26.157462625139736
80-81	23.817168831063952	24.43227373704257	25.545989526622098	26.204567905271375
82-83	23.66093687432737	24.383421766349684	25.638341448291662	26.317299911031284
84-85	23.605076269219722	24.353710299137692	25.5391253721693	26.502088059473294
86-87	23.411723954499625	24.390591666321708	25.65096205590054	26.546722323278125
88-89	23.305193705089195	24.129397472487337	25.76862696858997	26.796781853833494
90-91	23.322519138505307	24.01146563959599	25.740718102755757	26.92529711914295
92-93	23.265363858871538	24.00759375867717	25.725627681339645	27.00141470111165
94-95	23.098911650841664	23.889661811044345	25.823517002066502	27.187909536047485
96-97	22.921025821926467	23.786063248088833	25.91612419914314	27.376786730841555
98-99	22.734935762705557	23.711964864032577	25.918412421717107	27.634686951544758
100-101	22.98609534431051	23.488579980981676	25.93842413902186	27.586900535685956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	307.0
1	266.0
2	285.5
3	354.0
4	343.5
5	360.0
6	539.5
7	1114.5
8	1587.5
9	1570.5
10	1309.5
11	1112.5
12	1116.0
13	1167.5
14	1267.0
15	1356.0
16	1478.5
17	1627.0
18	1748.0
19	1895.0
20	2056.5
21	2281.0
22	2557.0
23	2827.0
24	3073.0
25	3469.5
26	4171.5
27	5010.0
28	6187.5
29	8025.0
30	10709.5
31	15100.0
32	22199.0
33	31233.0
34	41750.5
35	54471.5
36	70630.5
37	87485.5
38	103841.0
39	120136.5
40	134964.5
41	150365.0
42	167153.5
43	187336.0
44	208251.0
45	227795.0
46	247020.0
47	263958.5
48	280920.5
49	295063.0
50	301955.5
51	304953.0
52	307288.5
53	308002.5
54	304571.0
55	292929.5
56	273200.0
57	247789.5
58	223671.0
59	199788.5
60	170971.5
61	142663.5
62	115702.0
63	89608.0
64	66722.0
65	49091.0
66	36348.5
67	27302.0
68	20480.5
69	15143.0
70	11074.0
71	8038.0
72	5722.0
73	4105.5
74	2998.5
75	2106.5
76	1431.0
77	860.5
78	500.5
79	294.0
80	155.5
81	78.5
82	39.5
83	23.0
84	14.5
85	11.0
86	10.0
87	5.5
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24458753697548016
2	0.0
3	6.33277330508072E-5
4	0.013520471006347339
5	0.015689445863337485
6	0.10327170067260386
7	0.13339986967152537
8	0.1502925424628282
9	0.10867038991518518
10-11	0.11712464227746794
12-13	0.09280679278595796
14-15	0.11531980188551993
16-17	0.07054709461859923
18-19	0.07497211996552439
20-21	0.05193665706829326
22-23	0.07824933015090366
24-25	0.07046793495228572
26-27	0.049213564547108554
28-29	0.018293798885051935
30-31	0.03060312699680258
32-33	0.037933312097433516
34-35	0.042207934078363006
36-37	0.03513897587656665
38-39	0.03739502636650165
40-41	0.021246454438545816
42-43	0.030270656398285842
44-45	0.016971832457616333
46-47	0.029653211001040475
48-49	3.641344650421414E-4
50-51	0.01642563076005312
52-53	0.008105949830503322
54-55	0.016750185391938507
56-57	0.010844874284950734
58-59	0.007987210331033058
60-61	0.02996984966629451
62-63	0.013219664274356004
64-65	0.014161664303486763
66-67	0.056852472346362176
68-69	0.07549457376319353
70-71	0.032360471588962485
72-73	0.053551514261088846
74-75	0.04640339639297898
76-77	0.22320651110420134
78-79	0.08000667474306355
80-81	0.015871513095858554
82-83	0.010211596954442662
84-85	0.023961630993099177
86-87	0.1782200727382342
88-89	0.3824045160272993
90-91	0.2775021262286372
92-93	0.2769163446979172
94-95	0.002066067290782585
96-97	0.005992386739932631
98-99	0.004955395111225664
100-101	0.005287865709742401
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6316348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.09612654716877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81447004132184	39.4964022537924
2	12.423817043867324	12.94465489832369
3	4.442712251294301	6.943442989682549
4	2.189344412638566	4.562254543046226
5	1.239538395376455	3.2287574552803164
6	0.774627830028873	2.4213065676085748
7	0.5480883486291623	1.9987295978449517
8	0.38897700113000067	1.621135605984538
9	0.2937113752235072	1.3771102474788124
>10	1.7100857347211027	16.66876268422954
>50	0.1279954984864951	4.549321477669677
>100	0.04589817515540227	3.809189258553871
>500	6.421577119102148E-4	0.23243990455869828
>1k	9.173441508970938E-5	0.14649251594618634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.58319332627018E-5	0.0
3	0.0	0.0	0.0	1.58319332627018E-5	0.0
4	0.0	0.0	0.0	1.58319332627018E-5	0.0
5	0.0	1.58319332627018E-5	0.0	1.58319332627018E-5	0.0
6	0.0	1.58319332627018E-5	0.0	1.58319332627018E-5	7.915966631350901E-5
7	0.0	1.58319332627018E-5	0.0	1.58319332627018E-5	7.915966631350901E-5
8	0.0	1.58319332627018E-5	0.0	1.58319332627018E-5	9.499159957621081E-5
9	0.0	1.58319332627018E-5	0.0	3.16638665254036E-5	1.266554661016144E-4
10-11	0.0	1.58319332627018E-5	0.0	5.54117664194563E-5	1.5831933262701802E-4
12-13	0.0	1.58319332627018E-5	0.0	9.499159957621081E-5	2.6914286546593063E-4
14-15	0.0	2.3747899894052702E-5	0.0	1.108235328389126E-4	4.432941313556504E-4
16-17	0.0	3.16638665254036E-5	0.0	1.741512658897198E-4	5.066218644064576E-4
18-19	0.0	3.9579833156754506E-5	0.0	2.6914286546593063E-4	6.174453972453703E-4
20-21	0.0	6.33277330508072E-5	0.0	3.0872269862268514E-4	6.570252304021248E-4
22-23	0.0	6.33277330508072E-5	0.0	5.382857309318613E-4	7.045210301902301E-4
24-25	0.0	7.12436996821581E-5	0.0	0.0010211596954442662	7.757647298723883E-4
26-27	0.0	7.915966631350901E-5	0.0	0.0021214790572020416	8.470084295545465E-4
28-29	0.0	7.915966631350901E-5	0.0	0.006546504404127195	8.549243961858973E-4
30-31	0.0	7.915966631350901E-5	0.0	0.024563244457081843	8.628403628172482E-4
32-33	0.0	7.915966631350901E-5	0.0	0.0658450104395768	9.024201959740027E-4
34-35	0.0	7.915966631350901E-5	0.0	0.12174756679017687	9.657479290248099E-4
36-37	0.0	8.707563294485991E-5	0.0	0.20760414087380874	9.736638956561608E-4
38-39	0.0	9.499159957621081E-5	0.0	0.34508073336047984	0.0010053277621815643
40-41	0.0	9.499159957621081E-5	0.0	0.5011360995309315	0.0010290756620756172
42-43	0.0	1.5831933262701802E-4	0.0	0.6614581717156813	0.0010686554952323716
44-45	0.0	1.5831933262701802E-4	0.0	0.8521063120651364	0.0011398991949145296
46-47	7.9159666313509E-6	1.5831933262701802E-4	0.0	1.0731596802456103	0.0012190588612280388
48-49	1.58319332627018E-5	1.7415126588971982E-4	0.0	1.305912847107221	0.0012982185275415478
50-51	1.58319332627018E-5	1.8998319915242162E-4	0.0	1.5555349388602402	0.0013219664274356003
52-53	1.58319332627018E-5	2.137310990464743E-4	0.0	1.8097641231927057	0.0013852941604864076
54-55	1.58319332627018E-5	2.2956303230917612E-4	0.0	2.0917071067015307	0.001440705926905864
56-57	1.58319332627018E-5	2.533109322032288E-4	0.0	2.414013604063614	0.0014565378601685659
58-59	1.58319332627018E-5	2.8497479872863245E-4	0.0	2.7629177493070363	0.0014723697934312676
60-61	1.58319332627018E-5	3.008067319913342E-4	0.0	3.138071239900018	0.0015119496265880222
62-63	1.58319332627018E-5	3.008067319913342E-4	0.0	3.531502697444789	0.0016306891260582856
64-65	1.58319332627018E-5	3.324705985167378E-4	0.0	3.9556560214858334	0.0016386050926896364
66-67	1.58319332627018E-5	3.324705985167378E-4	0.0	4.424051683029497	0.0016544370259523383
68-69	1.58319332627018E-5	3.324705985167378E-4	0.0	4.946956690796644	0.001678184925846391
70-71	1.58319332627018E-5	3.4038656514808873E-4	0.0	5.516114691590774	0.0017098487923717946
72-73	1.58319332627018E-5	3.4830253177943964E-4	0.0	6.114886323552787	0.0019869076244690763
74-75	1.58319332627018E-5	3.5621849841079055E-4	0.0	6.738949468901966	0.0020185714909944797
76-77	1.58319332627018E-5	3.7996639830484324E-4	0.0	7.414197254489461	0.0020423193908885324
78-79	1.58319332627018E-5	3.7996639830484324E-4	0.0	8.149479731009121	0.0020423193908885324
80-81	1.58319332627018E-5	3.8788236493619415E-4	0.0	8.926328948310005	0.002058151324151234
82-83	1.58319332627018E-5	3.9579833156754506E-4	0.0	9.73447789767125	0.0021452269570960943
84-85	1.58319332627018E-5	3.9579833156754506E-4	0.0	10.568068763785655	0.002153142923727445
86-87	1.58319332627018E-5	4.1163026483024683E-4	0.0	11.43621282424591	0.0022006387235155504
88-89	1.58319332627018E-5	4.1163026483024683E-4	0.0	12.360742315021275	0.0022560504899350065
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11085	0.0	44.268024	1
GGTATCA	5970	0.0	43.44996	1
TCAACGC	14725	0.0	32.0265	4
TATCAAC	14915	0.0	31.902815	2
CAACGCA	15040	0.0	31.418636	5
ATCAACG	15490	0.0	30.536795	3
AACGCAG	16085	0.0	29.460886	6
GTACATG	23035	0.0	25.414648	1
TACATGG	23645	0.0	24.40178	2
ACGCAGA	19295	0.0	24.116686	7
ACATGGG	24600	0.0	22.64532	3
CGCAGAG	20540	0.0	22.377129	8
CATGGGG	19115	0.0	20.29847	4
GCAGAGT	22075	0.0	19.614416	9
AGAGTAC	18800	0.0	17.790108	10-11
ATGGGGG	10585	0.0	16.780035	5
GTGGTAT	3745	0.0	16.776035	1
GTATAGG	1210	0.0	16.5208	1
AGTACAT	17585	0.0	16.445969	2
GAGTACA	18570	0.0	16.403452	1
>>END_MODULE