FastQCFastQC Report
Mon 15 Aug 2016
SRR2921562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5859591
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97250.041.5464171
GGTATCA54000.040.6493231
TCAACGC126250.031.6952764
TATCAAC130450.030.4997622
ATCAACG132500.030.2700793
CAACGCA131700.030.2425795
AACGCAG141400.028.6224926
GTACATG240050.026.6274261
TACATGG246550.025.5665252
ACATGGG266600.023.539963
ACGCAGA168500.023.0878817
CGCAGAG177750.021.7025878
CATGGGG205250.020.6150444
AGTACAT174400.020.1265562
GAGTACA187200.019.837931
GCAGAGT192350.018.7894749
GTGTAGC36600.017.7282831
GTGGTAT33000.017.4310971
GTATAGG12500.017.3028051
AGAGTAC163100.017.15423410-11
Produced by FastQC (version 0.11.5)