##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921562_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5859591
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.71372626519496	31.0	28.0	31.0	15.0	34.0
2	27.684732432690268	31.0	28.0	33.0	12.0	34.0
3	27.776698066469145	31.0	28.0	33.0	15.0	34.0
4	31.145927591191946	35.0	32.0	37.0	16.0	37.0
5	31.05467634174467	35.0	32.0	37.0	16.0	37.0
6	31.13945853900042	35.0	32.0	37.0	15.0	37.0
7	30.926669113936452	35.0	32.0	37.0	11.0	37.0
8	30.866090312446723	35.0	32.0	37.0	10.0	37.0
9	32.067409994997945	37.0	32.0	39.0	10.0	39.0
10-11	31.830933336473485	37.0	31.0	39.0	10.0	39.0
12-13	31.623935441910536	37.0	31.0	39.0	10.0	39.0
14-15	32.29707892581581	37.5	31.0	40.0	8.0	41.0
16-17	32.1391591153717	37.0	31.0	40.0	7.5	41.0
18-19	31.941668880985038	37.0	30.5	40.0	2.0	41.0
20-21	31.794447257496298	37.0	30.0	40.0	2.0	41.0
22-23	31.558472852456767	37.0	30.0	40.0	2.0	41.0
24-25	31.647986437961286	37.0	30.0	40.0	2.0	41.0
26-27	31.391754305035967	37.0	30.0	40.0	2.0	41.0
28-29	31.183908313737255	37.0	30.0	40.0	2.0	41.0
30-31	30.921083997159528	37.0	29.0	40.0	2.0	41.0
32-33	30.664496208011787	36.0	29.0	40.0	2.0	41.0
34-35	30.314394810149718	36.0	27.5	39.5	2.0	41.0
36-37	30.000719333482493	36.0	27.0	39.0	2.0	41.0
38-39	29.764297286278172	35.5	26.5	39.0	2.0	40.0
40-41	29.513943379324598	35.0	26.0	39.0	2.0	40.0
42-43	29.246485974874354	35.0	25.5	39.0	2.0	40.0
44-45	28.96734447847981	35.0	24.5	39.0	2.0	40.0
46-47	28.65857087977642	35.0	24.0	39.0	2.0	40.0
48-49	28.346929845444844	34.0	23.5	38.5	2.0	40.0
50-51	27.43186341845361	33.0	22.5	37.5	2.0	39.5
52-53	27.63195528493371	33.5	23.0	37.5	2.0	39.5
54-55	28.171661810525684	34.0	23.0	38.5	2.0	40.0
56-57	28.127256578146834	34.0	23.0	39.0	2.0	40.0
58-59	27.86782405120084	34.0	23.0	38.0	2.0	40.0
60-61	27.50722388303211	34.0	22.0	38.0	2.0	40.0
62-63	27.071427681556614	33.5	20.5	37.5	2.0	40.0
64-65	26.587020152089114	33.0	19.5	37.0	2.0	39.5
66-67	26.22613088524438	33.0	18.0	36.5	2.0	39.0
68-69	25.761291786406254	32.0	15.0	36.0	2.0	39.0
70-71	25.32822913749441	32.0	11.5	35.5	2.0	38.5
72-73	24.848810949433158	31.5	7.5	35.0	2.0	37.5
74-75	24.401665747660545	31.0	4.5	35.0	2.0	37.0
76-77	23.9696677635009	31.0	2.0	35.0	2.0	36.5
78-79	23.57139380547209	31.0	2.0	34.5	2.0	36.0
80-81	23.13043197042251	30.5	2.0	34.0	2.0	35.5
82-83	22.593473247535535	30.0	2.0	34.0	2.0	35.0
84-85	22.17035429605923	30.0	2.0	34.0	2.0	35.0
86-87	21.66721713170766	29.0	2.0	34.0	2.0	35.0
88-89	21.150822813401142	29.0	2.0	34.0	2.0	35.0
90-91	20.558370200240937	29.0	2.0	34.0	2.0	35.0
92-93	20.010480338986117	27.5	2.0	34.0	2.0	35.0
94-95	19.323802036695053	26.5	2.0	33.0	2.0	35.0
96-97	18.677750802061098	25.0	2.0	33.0	2.0	35.0
98	18.19966018106042	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	438206.0
3	70860.0
4	50112.0
5	40501.0
6	37259.0
7	39026.0
8	44376.0
9	50015.0
10	52852.0
11	54352.0
12	55068.0
13	53368.0
14	51811.0
15	52794.0
16	56589.0
17	59908.0
18	62151.0
19	63154.0
20	65796.0
21	69566.0
22	75383.0
23	83104.0
24	93108.0
25	105692.0
26	120384.0
27	137910.0
28	157131.0
29	180170.0
30	207634.0
31	240041.0
32	282377.0
33	337540.0
34	410796.0
35	504153.0
36	599994.0
37	579043.0
38	260705.0
39	16662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12998112618069	16.79804157375983	12.958156614182329	25.113820685877165
2	16.480599517039007	18.53051733709638	38.86576296077514	26.123120185089466
3	20.55747497153351	22.185508905030442	27.22711220362286	30.029903919813194
4	14.074145222309879	14.459234415611938	34.320484721536694	37.14613564054149
5	15.750933842570985	35.96460720847679	32.54993154584036	15.734527403111857
6	35.813625648295215	32.461051062116475	16.486770116147593	15.23855317344072
7	31.823587883200066	30.019849555869175	18.92583273035712	19.230729830573644
8	28.248214079668127	33.40396449393064	18.659172048494828	19.68864937790641
9	27.73666104106417	15.854521509736935	18.163647001635795	38.245170447563105
10-11	27.14454001152835	24.335615821744895	25.827312806155167	22.69253136057159
12-13	27.521560220986927	21.382371564573138	26.23555896142286	24.860509253017067
14-15	24.704757865307815	22.954746877708228	23.535062817066922	28.805432439917034
16-17	25.116980064234923	26.022645387923575	24.16996039335613	24.690414154485367
18-19	25.1038818116544	25.37922944326862	25.48743557278642	24.02945317229056
20-21	25.854325161945262	24.46596055090048	25.28447465309891	24.395239634055347
22-23	25.644199187634275	24.123776859963012	25.127787435239245	25.10423651716347
24-25	25.19927224020309	24.474202599322695	25.121163773453674	25.205361387020535
26-27	25.072518962375206	24.65832621375045	25.224453693899818	25.044701129974527
28-29	25.11583962316938	24.838256782494188	25.069607139211286	24.976296455125148
30-31	25.380164496606106	24.65629948996538	24.92715529746696	25.03638071596156
32-33	25.055044849699414	24.7039800347463	24.953503030592707	25.287472084961575
34-35	25.39883720123864	24.7008565814463	25.101874811594634	24.798431405720432
36-37	25.32649666640928	24.664551997407756	24.99968933850011	25.009261997682852
38-39	25.467194139937256	24.644645625706843	24.922486633757988	24.965673600597917
40-41	25.39264616107389	24.61828506830048	24.919236547862937	25.069832222762688
42-43	25.251350609938093	24.452885105294005	24.921280974057858	25.374483310710044
44-45	25.150304522344126	24.57166711265721	25.032640085630188	25.245388279368473
46-47	25.286998231044272	24.448600390956603	24.970338285273293	25.29406309272584
48-49	25.190072434275812	24.59346218815375	24.91130780812397	25.305157569446475
50-51	25.080308041730554	24.690554794685944	25.041697500581854	25.187439663001648
52-53	25.16302971001586	24.61300764487834	25.099905682353345	25.124056962752455
54-55	24.879875983994143	24.427587959099114	25.356089017478787	25.336447039427952
56-57	25.000982786126297	24.540242372616145	25.171212477161465	25.287562364096093
58-59	25.049621370532837	24.47346545376453	25.229277276512025	25.247635899190612
60-61	25.208679391057725	24.36972285609345	25.268538795959326	25.153058956889495
62-63	24.99014126444096	24.41991456875771	25.318979870709907	25.27096429609142
64-65	24.87284845330856	24.296116342701662	25.398790178150062	25.432245025839716
66-67	24.907913241577262	24.49902713068318	25.23453895109678	25.358520676642776
68-69	24.893006742392814	24.3449491569547	25.256283287540555	25.505760813111934
70-71	24.874906844156104	24.20685584205392	25.360802589229493	25.55743472456048
72-73	24.85086192537096	24.375333717145498	25.26919493087187	25.504609426611673
74-75	24.73806408810615	24.454871624979578	25.35565301879828	25.451411268115994
76-77	24.761229370263806	24.16482700694081	25.449636116247664	25.624307506547716
78-79	24.699575102415537	24.13042015747967	25.50976668721454	25.660238052890254
80-81	24.737039125250714	24.303272641627956	25.348004232569494	25.611684000551836
82-83	24.787714523709177	24.34376552426347	25.315389695603752	25.5531302564236
84-85	24.804983788053782	24.348558769321357	25.274417732877964	25.572039709746896
86-87	24.619641914229547	24.320769265659727	25.48519415211585	25.574394667994877
88-89	24.618845896311154	24.119058107521145	25.465576328494393	25.796519667673305
90-91	24.67569403291399	24.0380714596028	25.544974729605237	25.74125977787797
92-93	24.740244708640965	24.145199213543485	25.4932661265305	25.621289951285053
94-95	24.704472705354984	24.07514999545507	25.531863538501014	25.688513760688924
96-97	24.573726228562204	24.061899845713434	25.583881273465874	25.780492652258484
98	24.717223722994024	23.930720775020202	25.48477706135206	25.86727844063371
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	89.5
2	148.0
3	213.0
4	240.5
5	322.0
6	474.0
7	809.0
8	1166.0
9	1292.0
10	1340.5
11	1492.0
12	1743.0
13	1952.5
14	2186.5
15	2484.0
16	2553.0
17	2545.0
18	2598.5
19	2465.5
20	2331.0
21	2280.5
22	2236.0
23	2330.5
24	2596.5
25	2914.0
26	3410.0
27	4035.0
28	4956.0
29	6852.0
30	10031.0
31	14952.0
32	22193.0
33	31057.5
34	42383.5
35	55895.0
36	70839.0
37	85976.5
38	99587.0
39	113079.0
40	127497.0
41	142565.0
42	160685.0
43	181386.0
44	200214.5
45	219769.5
46	240054.5
47	257673.5
48	271817.5
49	282840.0
50	287892.0
51	287620.0
52	288737.5
53	291324.5
54	288540.0
55	280111.5
56	265510.0
57	245263.0
58	223068.0
59	199349.0
60	174041.0
61	146118.0
62	118921.5
63	92845.0
64	69474.5
65	51552.5
66	38038.5
67	28099.0
68	20874.5
69	15679.5
70	11787.0
71	8764.0
72	6614.0
73	4956.0
74	3777.0
75	2800.0
76	1880.5
77	1188.5
78	732.0
79	429.5
80	249.5
81	159.5
82	115.0
83	72.0
84	51.5
85	48.5
86	39.5
87	26.0
88	15.0
89	18.5
90	19.0
91	14.5
92	15.5
93	16.5
94	15.5
95	8.0
96	4.5
97	5.5
98	3.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31002163802900234
2	0.1538503284614916
3	0.1510856303793217
4	0.14470293233776896
5	0.14045348898924856
6	0.05251219752368383
7	0.04322827309960712
8	0.016844179056183274
9	0.011314782891843475
10-11	0.0021076556367159416
12-13	0.007184801806132885
14-15	0.012176617787828536
16-17	0.008072235758434333
18-19	0.05420173524056542
20-21	0.10754163558514579
22-23	0.10778909312953754
24-25	0.08382837641739842
26-27	0.09271124895918503
28-29	0.11201293742174157
30-31	0.08847887164820889
32-33	0.02412284406880958
34-35	0.3320624255174124
36-37	0.4311990376120108
38-39	0.39831278326422437
40-41	0.394259599347463
42-43	0.3682936232238735
44-45	0.35034015172731336
46-47	0.35557942525340075
48-49	0.3487530102356974
50-51	0.3500585621078331
52-53	0.400394839844624
54-55	0.3857351135941058
56-57	0.3685666798245816
58-59	0.3476863828891812
60-61	0.35684231203167593
62-63	0.35600607619200725
64-65	0.37326837316802486
66-67	0.39673417479138046
68-69	0.35477732148882063
70-71	0.32841029348294104
72-73	0.3104653550051531
74-75	0.2948072655582958
76-77	0.2982802041985524
78-79	0.306301241844354
80-81	0.2948499306521564
82-83	0.30597698713101307
84-85	0.2977767560909968
86-87	0.292443619358416
88-89	0.3193396945281676
90-91	0.299969741915434
92-93	0.2889024165679823
94-95	0.3057209965678492
96-97	0.30486769469063624
98	0.30346827961200706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5859591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.572542890137974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40803272493228	39.40556544857637
2	11.007314602566353	11.353504088921907
3	4.611825223756435	7.135306624619966
4	2.288608802732366	4.721175025506491
5	1.4113462745719783	3.63933581390999
6	0.9032765264460955	2.795056044107767
7	0.6217412551841371	2.2445344284686493
8	0.46478324268641846	1.917604297445018
9	0.3248474408031925	1.5077887716216785
>10	1.793788346359141	16.66341492509707
>50	0.11586665139304644	4.079129754659869
>100	0.04750087284961568	4.081169234889397
>500	8.344059233878953E-4	0.2770421048106918
>1k	2.3362979565581089E-4	0.17937343736505362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.7066037544258635E-5	0.0	0.0	0.0
6	0.0	1.7066037544258635E-5	0.0	0.0	0.0
7	0.0	1.7066037544258635E-5	0.0	0.0	0.0
8	0.0	1.7066037544258635E-5	0.0	0.0	1.7066037544258635E-5
9	0.0	1.7066037544258635E-5	0.0	1.7066037544258635E-5	1.7066037544258635E-5
10-11	0.0	1.7066037544258635E-5	0.0	1.7066037544258635E-5	1.7066037544258635E-5
12-13	0.0	1.7066037544258635E-5	0.0	3.413207508851727E-5	1.7066037544258635E-5
14-15	0.0	1.7066037544258635E-5	0.0	3.413207508851727E-5	3.413207508851727E-5
16-17	0.0	1.7066037544258635E-5	0.0	7.679716894916385E-5	5.11981126327759E-5
18-19	0.0	1.7066037544258635E-5	0.0	1.1946226280981044E-4	5.11981126327759E-5
20-21	0.0	1.7066037544258635E-5	0.0	1.2799528158193976E-4	5.11981126327759E-5
22-23	0.0	1.7066037544258635E-5	0.0	2.2185848807536226E-4	5.11981126327759E-5
24-25	0.0	1.7066037544258635E-5	0.0	3.925188635179486E-4	5.11981126327759E-5
26-27	0.0	1.7066037544258635E-5	0.0	8.959669710735783E-4	5.11981126327759E-5
28-29	0.0	1.7066037544258635E-5	0.0	0.003362009396218951	7.679716894916385E-5
30-31	0.0	1.7066037544258635E-5	0.0	0.011852363074487622	8.533018772129318E-5
32-33	0.0	2.559905631638795E-5	0.0	0.029805834571047705	8.533018772129318E-5
34-35	0.0	3.413207508851727E-5	0.0	0.05417613618424903	8.533018772129318E-5
36-37	0.0	3.413207508851727E-5	8.533018772129318E-6	0.09715695173946441	8.533018772129318E-5
38-39	0.0	3.413207508851727E-5	1.7066037544258635E-5	0.1669485122767101	8.533018772129318E-5
40-41	0.0	3.413207508851727E-5	1.7066037544258635E-5	0.24869483211370896	8.533018772129318E-5
42-43	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.33031315666912586	9.386320649342249E-5
44-45	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.4289633866937129	1.023962252655518E-4
46-47	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.5461473334913648	1.023962252655518E-4
48-49	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.6772742329626761	1.023962252655518E-4
50-51	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.8174461323324445	1.023962252655518E-4
52-53	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	0.9662107816057468	1.023962252655518E-4
54-55	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	1.134874430655655	1.1092924403768113E-4
56-57	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	1.3313557209027045	1.1946226280981044E-4
58-59	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	1.547206963762488	1.1946226280981044E-4
60-61	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	1.7842371592146962	1.1946226280981044E-4
62-63	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	2.0258325197099936	1.1946226280981044E-4
64-65	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	2.286251719616608	1.1946226280981044E-4
66-67	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	2.5876208766106714	1.1946226280981044E-4
68-69	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	2.9259294718692823	1.1946226280981044E-4
70-71	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	3.2935831186852464	1.1946226280981044E-4
72-73	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	3.674642137992225	1.1946226280981044E-4
74-75	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	4.068381223194589	1.1946226280981044E-4
76-77	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	4.500570090984166	1.1946226280981044E-4
78-79	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	4.9839997365003805	1.1946226280981044E-4
80-81	1.7066037544258635E-5	3.413207508851727E-5	1.7066037544258635E-5	5.49730348073782	1.3652830035406908E-4
82-83	1.7066037544258635E-5	5.11981126327759E-5	1.7066037544258635E-5	6.036888922793417	1.7066037544258636E-4
84-85	3.413207508851727E-5	5.11981126327759E-5	1.7066037544258635E-5	6.587686751515593	1.7066037544258636E-4
86	3.413207508851727E-5	5.11981126327759E-5	1.7066037544258635E-5	7.0163941476461416	1.7066037544258636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9725	0.0	41.546417	1
GGTATCA	5400	0.0	40.649323	1
TCAACGC	12625	0.0	31.695276	4
TATCAAC	13045	0.0	30.499762	2
ATCAACG	13250	0.0	30.270079	3
CAACGCA	13170	0.0	30.242579	5
AACGCAG	14140	0.0	28.622492	6
GTACATG	24005	0.0	26.627426	1
TACATGG	24655	0.0	25.566525	2
ACATGGG	26660	0.0	23.53996	3
ACGCAGA	16850	0.0	23.087881	7
CGCAGAG	17775	0.0	21.702587	8
CATGGGG	20525	0.0	20.615044	4
AGTACAT	17440	0.0	20.126556	2
GAGTACA	18720	0.0	19.83793	1
GCAGAGT	19235	0.0	18.789474	9
GTGTAGC	3660	0.0	17.728283	1
GTGGTAT	3300	0.0	17.431097	1
GTATAGG	1250	0.0	17.302805	1
AGAGTAC	16310	0.0	17.154234	10-11
>>END_MODULE