FastQCFastQC Report
Mon 15 Aug 2016
SRR2921562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5859591
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.044.6769331
GTATCAA95800.044.4666441
TCAACGC131200.032.071664
TATCAAC136750.031.255172
CAACGCA135350.030.8780385
ATCAACG138850.030.4414773
AACGCAG144350.029.0797216
GTACATG235650.026.8634011
TACATGG248050.025.2146682
ACGCAGA174750.023.5046467
ACATGGG263550.023.264023
CGCAGAG184600.021.8131778
CATGGGG204550.020.4781484
GAGTACA185600.019.0540641
AGTACAT176850.018.9854582
GCAGAGT203500.018.7816739
GTGGTAT30450.018.1320721
AGAGTAC164300.018.06075710-11
GTATAGG15200.016.9093511
GTATTAG12150.016.4531781
Produced by FastQC (version 0.11.5)