##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5859591
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.93426640187003	31.0	30.0	34.0	26.0	34.0
2	29.924288231038652	31.0	30.0	34.0	26.0	34.0
3	30.101822294422938	31.0	30.0	34.0	26.0	34.0
4	33.74225538949732	35.0	35.0	37.0	30.0	37.0
5	33.228481134604785	35.0	33.0	37.0	27.0	37.0
6	33.113225650049635	35.0	33.0	37.0	27.0	37.0
7	32.916916385461036	35.0	33.0	37.0	26.0	37.0
8	32.804145374651576	35.0	33.0	37.0	26.0	37.0
9	34.1150501801235	37.0	34.0	39.0	26.0	39.0
10-11	33.8974762061038	37.0	33.0	39.0	25.0	39.0
12-13	33.70850670294223	37.0	33.0	39.0	25.0	39.0
14-15	34.56109103860662	38.0	33.0	40.0	24.0	41.0
16-17	34.40706254071317	38.0	33.0	40.0	23.5	41.0
18-19	34.261950791446026	38.0	33.0	40.0	22.5	41.0
20-21	34.11231859015416	38.0	33.0	40.0	20.0	41.0
22-23	33.93178662128466	38.0	33.0	40.0	19.0	41.0
24-25	33.76047611514183	38.0	32.0	40.0	18.0	41.0
26-27	33.662134353745856	38.0	32.5	40.0	18.0	41.0
28-29	33.66935021574031	38.0	32.0	40.0	18.0	41.0
30-31	33.504377694620665	38.0	32.0	40.0	17.0	41.0
32-33	33.329953745918445	38.0	32.0	40.0	16.0	41.0
34-35	33.11583299926565	38.0	32.0	40.0	16.0	41.0
36-37	32.932366098589476	38.0	31.0	40.0	15.0	41.0
38-39	32.7375236599278	37.5	31.0	40.0	13.5	41.0
40-41	32.740347235839494	38.0	31.0	40.0	11.0	41.0
42-43	32.52312251827816	37.0	31.0	40.0	9.0	41.0
44-45	32.30584805321736	37.0	31.0	40.0	8.5	41.0
46-47	32.02241504569176	37.0	30.0	40.0	7.5	41.0
48-49	31.712236826768283	37.0	30.0	40.0	4.0	41.0
50-51	31.420074455708598	36.0	30.0	40.0	2.0	41.0
52-53	31.078125759971982	36.0	29.0	39.0	2.0	41.0
54-55	30.73549186282797	35.5	28.5	39.0	2.0	40.0
56-57	30.371802741863725	35.0	28.0	39.0	2.0	40.0
58-59	29.874042744621597	35.0	28.0	38.5	2.0	40.0
60-61	29.501498056775635	34.0	27.0	38.0	2.0	40.0
62-63	29.147233655045206	34.0	26.5	38.0	2.0	40.0
64-65	28.675314966522407	34.0	26.0	37.0	2.0	40.0
66-67	28.24069205512808	34.0	26.0	37.0	2.0	39.0
68-69	27.79897709584167	33.0	25.5	36.0	2.0	39.0
70-71	27.329614046441126	33.0	24.5	36.0	2.0	39.0
72-73	26.850878670542023	32.5	24.0	35.0	2.0	38.0
74-75	26.32118120530938	32.0	22.5	35.0	2.0	37.0
76-77	24.876631150535935	30.5	20.0	34.0	2.0	35.5
78-79	25.74116836823594	31.5	21.5	34.5	2.0	36.0
80-81	25.7975946614704	32.0	21.5	35.0	2.0	36.0
82-83	25.48454644701311	32.0	20.0	35.0	2.0	35.5
84-85	25.066471277602822	32.0	19.0	34.5	2.0	35.0
86-87	24.62633262287419	31.0	17.5	34.0	2.0	35.0
88-89	24.183648568645832	31.0	11.5	34.0	2.0	35.0
90-91	23.755934074579606	31.0	7.0	34.0	2.0	35.0
92-93	23.34381205104588	31.0	2.0	34.0	2.0	35.0
94-95	22.953732094953384	31.0	2.0	34.0	2.0	35.0
96-97	22.41046593525043	30.0	2.0	34.0	2.0	35.0
98-99	21.636331528941184	30.0	2.0	34.0	2.0	35.0
100-101	19.071473247876856	25.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	175734.0
3	77038.0
4	40293.0
5	29123.0
6	25219.0
7	25030.0
8	28357.0
9	32543.0
10	35897.0
11	40322.0
12	44161.0
13	47693.0
14	51362.0
15	54088.0
16	56986.0
17	58614.0
18	57752.0
19	56980.0
20	57342.0
21	59571.0
22	64261.0
23	71208.0
24	81840.0
25	96055.0
26	112856.0
27	133202.0
28	156502.0
29	182896.0
30	213689.0
31	251963.0
32	299113.0
33	362589.0
34	450652.0
35	567675.0
36	716094.0
37	725777.0
38	305960.0
39	13154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20020296395114	18.083191611553275	13.63310775890606	25.083497665589515
2	16.49929150345135	19.559709884188162	37.61240332303057	26.32859528932992
3	20.38946062959002	22.87342239415686	27.546871445464365	29.190245530788754
4	14.667628195554494	15.343833342578803	33.87726851832096	36.111269943545736
5	15.753450144027681	36.194388553161886	32.58898548389409	15.46317581891634
6	35.88273915479155	32.97445315021321	16.19097018096233	14.951837514032906
7	31.73861907566274	30.154848041857917	18.9824203175506	19.12411256492875
8	28.278485162525858	33.65570318590639	18.518284917176075	19.547526734391685
9	27.560594114044136	15.717711258250992	18.111132847037076	38.6105617806678
10-11	27.08318862933726	24.482882382285275	25.890562856041242	22.543366132336214
12-13	27.465851929051478	21.40631066908029	26.324920895846954	24.802916506021276
14-15	24.600416689057134	23.102774330334277	23.502035133139476	28.794773847469113
16-17	25.08403928955269	26.149194481509646	24.203298312621886	24.563467916315773
18-19	25.084208452502143	25.492246793298236	25.51731099308971	23.90623376110991
20-21	25.781480118606197	24.48091859185449	25.32997914231634	24.407622147222973
22-23	25.543775023027887	24.238115465501547	25.213476605833414	25.00463290563715
24-25	25.20723064371427	24.517107981618363	25.174652714147154	25.101008660520215
26-27	25.054289456115097	24.753857633392926	25.24242005132697	24.949432859165004
28-29	25.047924140700378	24.89576003321682	25.156416492168827	24.899899333913975
30-31	25.355182329190978	24.67650694334368	25.0096860342111	24.958624693254244
32-33	25.045815140730248	24.72816520562436	24.987238003467166	25.238781650178222
34-35	25.295981963162266	24.78865748576146	25.176814877830044	24.738545673246225
36-37	25.31530438741929	24.73988652465076	25.002195941865292	24.942613146064662
38-39	25.377801725352167	24.657897688655016	25.024000215801333	24.940300370191483
40-41	25.305681943295788	24.63087809678413	25.03406707924405	25.029372880676032
42-43	25.157053022322668	24.568976444586063	25.002136093527522	25.271834439563744
44-45	25.042975731662793	24.619013375177666	25.135899923171973	25.20211096998757
46-47	25.146456646673066	24.527656882290877	25.062781766814336	25.263104704221718
48-49	25.038654713571134	24.643992647924676	25.056471720621136	25.260880917883057
50-51	25.022176849127614	24.744908627285042	25.034671329451964	25.19824319413538
52-53	25.120255274766244	24.64378265700773	25.068609104284555	25.167352963941465
54-55	24.895529541729637	24.48835846962599	25.218755558074175	25.397356430570202
56-57	24.98910634477544	24.63049899170751	25.01251511585336	25.36787954766369
58-59	25.02367685668969	24.54935461897968	25.105447136307863	25.32152138802276
60-61	25.203208174095586	24.515543535719374	25.064579257696394	25.216669032488642
62-63	24.950546541893495	24.559049421710945	25.120991248792517	25.36941278760304
64-65	24.833455274252707	24.399665353038806	25.185298849414245	25.581580523294246
66-67	24.846963152647852	24.612208180657934	25.046165489490797	25.49466317720342
68-69	24.870615831409637	24.478116646091813	24.998958163252063	25.652309359246487
70-71	24.809233186336506	24.292179258450055	25.119248731073657	25.77933882413978
72-73	24.777880003975213	24.450736250604905	24.963483732203333	25.80790001321655
74-75	24.60402796977849	24.487778908747686	25.068144897364554	25.840048224109267
76-77	24.61993801601649	24.157683709952998	25.32718282812059	25.89519544590992
78-79	24.47974164349573	24.18669427452138	25.347431717144854	25.98613236483804
80-81	24.482515567354245	24.360064243419714	25.156970768629556	26.00044942059649
82-83	24.46402614651054	24.357232895905426	25.15344595979047	26.02529499779356
84-85	24.4462667951606	24.315320426413	25.130461998624643	26.107950779801758
86-87	24.21004607273844	24.30295920063081	25.31520708112755	26.17178764550321
88-89	24.168291337449546	24.14564302699295	25.292612059454385	26.39345357610312
90-91	24.193220881109355	24.06175365674236	25.346605327845324	26.39842013430296
92-93	24.14842709001127	24.118058695457968	25.32333055976079	26.41018365476997
94-95	23.98045795617316	24.060832133423695	25.373579073462118	26.58513083694103
96-97	23.768655484149768	24.03597704854157	25.47217124145292	26.723196225855745
98-99	23.76839047228296	23.947627389285106	25.44894563909174	26.83503649934019
100-101	24.0331413214083	23.63923569679268	25.469975234975884	26.857647746823133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	76.0
2	77.0
3	102.5
4	103.5
5	118.5
6	221.0
7	477.5
8	697.5
9	740.5
10	655.0
11	546.0
12	561.0
13	607.0
14	664.5
15	776.0
16	904.0
17	995.0
18	1100.0
19	1202.0
20	1274.0
21	1436.5
22	1617.5
23	1817.5
24	2082.5
25	2440.5
26	2886.0
27	3499.0
28	4335.5
29	5680.0
30	7988.0
31	11712.5
32	17967.0
33	25833.5
34	34544.5
35	45148.5
36	59293.0
37	74587.5
38	87411.0
39	99619.5
40	112895.0
41	126506.0
42	141212.0
43	160414.5
44	180903.5
45	199671.0
46	217850.5
47	236204.0
48	255294.5
49	270003.5
50	279116.0
51	283224.0
52	285753.5
53	289351.5
54	287876.0
55	280697.5
56	266802.0
57	245414.5
58	223224.0
59	202063.0
60	177127.5
61	148852.5
62	121524.0
63	94900.0
64	70620.0
65	52118.5
66	38501.0
67	28373.0
68	21031.0
69	15677.5
70	11922.5
71	8949.5
72	6655.5
73	5209.0
74	3958.5
75	2862.5
76	2011.5
77	1242.5
78	698.5
79	414.5
80	262.0
81	159.5
82	84.5
83	46.5
84	26.0
85	14.5
86	11.0
87	5.0
88	3.5
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24264492180426928
2	0.0
3	0.0
4	0.014779188513327978
5	0.016980707356537342
6	0.10894958368254713
7	0.13954898899940285
8	0.15646143220576317
9	0.11103164026294668
10-11	0.12162111655915916
12-13	0.09556981024784836
14-15	0.11989744676718904
16-17	0.07328156521504658
18-19	0.07895602269851257
20-21	0.05522569749322094
22-23	0.08079062173452037
24-25	0.07509056519473799
26-27	0.05155649942120533
28-29	0.01900303280553199
30-31	0.032945985479191295
32-33	0.0402246504918176
34-35	0.04588204193773934
36-37	0.03746848542841984
38-39	0.04013932030409631
40-41	0.02211758465735919
42-43	0.03303131566691259
44-45	0.017953471496560083
46-47	0.030710334560893414
48-49	3.5838678842943137E-4
50-51	0.01713430169443567
52-53	0.008405023490547378
54-55	0.017014839431625857
56-57	0.011536641379918838
58-59	0.008430622546863767
60-61	0.03185375907635874
62-63	0.013644297016634778
64-65	0.01500958002017547
66-67	0.05947514084174134
68-69	0.07759927271374402
70-71	0.033816353393948485
72-73	0.05626672578342072
74-75	0.04726439097882429
76-77	0.22598846916107285
78-79	0.08081622079083677
80-81	0.01598234416019821
82-83	0.010350551770592862
84-85	0.025044410096199548
86-87	0.1805416111807121
88-89	0.3841735711588061
90-91	0.2783385393280862
92-93	0.2789443836609074
94-95	0.0020820565803995536
96-97	0.006066976346983945
98-99	0.005239273526087401
100-101	0.005452598995390634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5859591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.50012850942758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21509228681991	33.58011071657298
2	12.650680764496745	11.765165625616843
3	5.362829295775051	7.481167542829886
4	2.8398119421149937	5.282064810038173
5	1.7141017239453418	3.985297522084487
6	1.1073807459241745	3.089600819660393
7	0.7601034016492356	2.4741434099999755
8	0.5597120735341166	2.082134667809168
9	0.4073209159516318	1.7046427442695764
>10	2.168862419940508	18.381201961148047
>50	0.14916510562126903	4.718696302629739
>100	0.06338976581405355	4.8838502585286365
>500	0.0012913012188671695	0.39571738431922077
>1k	2.582571940984679E-4	0.17620623449275874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.7066037544258635E-5	0.0
3	0.0	0.0	0.0	1.7066037544258635E-5	0.0
4	0.0	0.0	0.0	1.7066037544258635E-5	0.0
5	0.0	0.0	0.0	1.7066037544258635E-5	0.0
6	0.0	0.0	0.0	1.7066037544258635E-5	1.7066037544258635E-5
7	1.7066037544258635E-5	0.0	0.0	1.7066037544258635E-5	1.7066037544258635E-5
8	1.7066037544258635E-5	0.0	0.0	1.7066037544258635E-5	1.7066037544258635E-5
9	1.7066037544258635E-5	0.0	0.0	1.7066037544258635E-5	3.413207508851727E-5
10-11	1.7066037544258635E-5	0.0	0.0	3.413207508851727E-5	3.413207508851727E-5
12-13	1.7066037544258635E-5	0.0	0.0	5.11981126327759E-5	3.413207508851727E-5
14-15	1.7066037544258635E-5	0.0	0.0	5.11981126327759E-5	3.413207508851727E-5
16-17	1.7066037544258635E-5	0.0	0.0	8.533018772129318E-5	3.413207508851727E-5
18-19	1.7066037544258635E-5	0.0	0.0	1.535943378983277E-4	3.413207508851727E-5
20-21	1.7066037544258635E-5	0.0	0.0	1.7919339421471568E-4	3.413207508851727E-5
22-23	1.7066037544258635E-5	0.0	0.0	2.7305660070813816E-4	5.11981126327759E-5
24-25	1.7066037544258635E-5	8.533018772129318E-6	0.0	4.437169761507245E-4	6.826415017703454E-5
26-27	1.7066037544258635E-5	1.7066037544258635E-5	0.0	9.13033008617837E-4	6.826415017703454E-5
28-29	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.003464405621484503	6.826415017703454E-5
30-31	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.011963292318525304	6.826415017703454E-5
32-33	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.029976494946490292	6.826415017703454E-5
34-35	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.05418466920302117	6.826415017703454E-5
36-37	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.09672176778208583	6.826415017703454E-5
38-39	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.16664132360091344	6.826415017703454E-5
40-41	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.24881429437651875	8.533018772129318E-5
42-43	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.3301424962936833	9.386320649342249E-5
44-45	1.7066037544258635E-5	1.7066037544258635E-5	0.0	0.4295350989514456	1.023962252655518E-4
46-47	2.559905631638795E-5	1.7066037544258635E-5	0.0	0.5459169419845173	1.023962252655518E-4
48-49	3.413207508851727E-5	3.413207508851727E-5	0.0	0.6756700254335157	1.1946226280981044E-4
50-51	3.413207508851727E-5	3.413207508851727E-5	0.0	0.8137342691665681	1.1946226280981044E-4
52-53	3.413207508851727E-5	3.413207508851727E-5	0.0	0.9595038288508533	1.2799528158193976E-4
54-55	3.413207508851727E-5	3.413207508851727E-5	0.0	1.1248140015233146	1.3652830035406908E-4
56-57	4.266509386064659E-5	3.413207508851727E-5	0.0	1.3176175606795764	1.3652830035406908E-4
58-59	5.11981126327759E-5	3.413207508851727E-5	0.0	1.5291937611345228	1.535943378983277E-4
60-61	5.11981126327759E-5	3.413207508851727E-5	0.0	1.7617099896562747	1.6212735667045703E-4
62-63	5.11981126327759E-5	3.413207508851727E-5	0.0	1.998748718127255	1.7066037544258636E-4
64-65	5.11981126327759E-5	3.413207508851727E-5	0.0	2.253629988850758	1.7066037544258636E-4
66-67	5.11981126327759E-5	3.413207508851727E-5	0.0	2.5487188440285333	1.7919339421471568E-4
68-69	6.826415017703454E-5	3.413207508851727E-5	0.0	2.8807812695459463	1.8772641298684498E-4
70-71	6.826415017703454E-5	3.413207508851727E-5	0.0	3.2427007277470388	1.8772641298684498E-4
72-73	6.826415017703454E-5	3.413207508851727E-5	0.0	3.6178890301387927	1.8772641298684498E-4
74-75	6.826415017703454E-5	3.413207508851727E-5	0.0	4.008086912550722	1.8772641298684498E-4
76-77	6.826415017703454E-5	3.413207508851727E-5	0.0	4.437903601121648	1.8772641298684498E-4
78-79	6.826415017703454E-5	3.413207508851727E-5	0.0	4.918261359879896	1.8772641298684498E-4
80-81	6.826415017703454E-5	3.413207508851727E-5	0.0	5.4289710664106074	1.8772641298684498E-4
82-83	6.826415017703454E-5	6.826415017703454E-5	0.0	5.966457385848262	2.2185848807536226E-4
84-85	6.826415017703454E-5	6.826415017703454E-5	0.0	6.516751766462881	2.2185848807536226E-4
86-87	6.826415017703454E-5	6.826415017703454E-5	0.0	7.093575302440051	2.2185848807536226E-4
88-89	6.826415017703454E-5	6.826415017703454E-5	0.0	7.717262177513755	2.2185848807536226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5135	0.0	44.676933	1
GTATCAA	9580	0.0	44.466644	1
TCAACGC	13120	0.0	32.07166	4
TATCAAC	13675	0.0	31.25517	2
CAACGCA	13535	0.0	30.878038	5
ATCAACG	13885	0.0	30.441477	3
AACGCAG	14435	0.0	29.079721	6
GTACATG	23565	0.0	26.863401	1
TACATGG	24805	0.0	25.214668	2
ACGCAGA	17475	0.0	23.504646	7
ACATGGG	26355	0.0	23.26402	3
CGCAGAG	18460	0.0	21.813177	8
CATGGGG	20455	0.0	20.478148	4
GAGTACA	18560	0.0	19.054064	1
AGTACAT	17685	0.0	18.985458	2
GCAGAGT	20350	0.0	18.781673	9
GTGGTAT	3045	0.0	18.132072	1
AGAGTAC	16430	0.0	18.060757	10-11
GTATAGG	1520	0.0	16.909351	1
GTATTAG	1215	0.0	16.453178	1
>>END_MODULE