FastQCFastQC Report
Mon 15 Aug 2016
SRR2921551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425401
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25800.075.66641
GTATCAA53250.060.803441
TCAACGC68550.046.173394
CAACGCA71100.044.350035
AACGCAG75300.041.7139936
ATCAACG76350.041.6086853
TATCAAC83050.038.8249932
GTACATG129450.037.057991
ACGCAGA87350.036.027627
TACATGG134750.035.319372
ACATGGG137150.034.4410553
CGCAGAG92700.033.819318
CATGGGG110650.032.3692554
GCAGAGT100050.030.3834929
AGAGTAC92050.026.04417810-11
ATGGGGG54350.025.8345015
CAGAGTA96700.025.80684310-11
ATGGGGA44150.024.256575
CATGGGT26500.022.0022854
GTATAGG8250.021.6429371
Produced by FastQC (version 0.11.5)