##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921551_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2425401
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.119064022815195	31.0	31.0	34.0	26.0	34.0
2	30.237807273931196	31.0	31.0	34.0	26.0	34.0
3	30.312888054387706	31.0	31.0	34.0	26.0	34.0
4	33.70806559410176	37.0	35.0	37.0	28.0	37.0
5	33.74590634703292	37.0	35.0	37.0	28.0	37.0
6	33.75474241166718	37.0	35.0	37.0	28.0	37.0
7	33.590504003255546	37.0	35.0	37.0	28.0	37.0
8	33.53976229085417	37.0	35.0	37.0	27.0	37.0
9	35.033081539918555	39.0	35.0	39.0	27.0	39.0
10-11	34.919600923723536	39.0	35.0	39.0	27.0	39.0
12-13	34.7825295693372	39.0	35.0	39.0	27.0	39.0
14-15	35.95657584044865	40.0	36.0	41.0	27.0	41.0
16-17	35.873195607654154	40.0	36.0	41.0	27.0	41.0
18-19	35.88749674796044	40.0	36.0	41.0	27.0	41.0
20-21	35.90870602428217	40.0	36.0	41.0	27.0	41.0
22-23	35.86551089902247	40.0	36.0	41.0	26.5	41.0
24-25	35.82268664027103	40.0	36.0	41.0	26.0	41.0
26-27	35.74631370235272	40.0	36.0	41.0	26.0	41.0
28-29	35.696223634772146	40.0	35.5	41.0	26.0	41.0
30-31	35.60019683343084	40.0	35.0	41.0	26.0	41.0
32-33	35.485642580340325	40.0	35.0	41.0	25.0	41.0
34-35	35.310363523392624	40.0	35.0	41.0	25.0	41.0
36-37	35.17206762922915	40.0	35.0	41.0	24.5	41.0
38-39	35.035656784177135	39.5	35.0	41.0	24.0	41.0
40-41	34.87822776522316	39.0	35.0	41.0	23.5	41.0
42-43	34.676094179890256	39.0	34.0	41.0	22.5	41.0
44-45	34.48463738573539	39.0	34.0	41.0	22.0	41.0
46-47	34.22968160728885	39.0	33.0	41.0	20.0	41.0
48-49	34.077480383656145	39.0	33.0	41.0	19.5	41.0
50-51	33.87529093127281	38.0	33.0	40.5	18.0	41.0
52-53	33.67785574426662	38.0	33.0	40.0	18.0	41.0
54-55	33.459615337834855	38.0	33.0	40.0	16.0	41.0
56-57	33.210373253742375	37.0	33.0	40.0	14.5	41.0
58-59	32.963453053742455	37.0	32.5	40.0	13.5	41.0
60-61	32.651079347291436	36.0	32.0	40.0	12.0	41.0
62-63	32.339508600845804	36.0	32.0	39.0	8.5	41.0
64-65	32.02982475887492	35.0	31.5	39.0	8.0	41.0
66-67	31.984930120833628	35.0	32.0	39.0	7.5	41.0
68-69	31.739119428086326	35.0	32.0	38.5	7.0	40.5
70-71	31.44771792375776	35.0	31.5	37.0	4.0	40.0
72-73	31.127335850855175	35.0	31.0	37.0	2.0	39.0
74-75	30.832534908660463	35.0	31.0	36.0	2.0	39.0
76-77	30.55932132459746	35.0	31.0	36.0	2.0	38.5
78-79	30.29563503107321	35.0	31.0	35.5	2.0	37.0
80-81	30.05931349084131	35.0	31.0	35.0	2.0	37.0
82-83	29.87056697016287	35.0	31.0	35.0	2.0	36.5
84-85	29.67827010873666	35.0	30.0	35.0	2.0	36.0
86-87	29.534470382423358	35.0	30.0	35.0	2.0	36.0
88-89	29.416989809107854	35.0	30.0	35.0	2.0	35.5
90-91	29.316349543848624	34.5	30.0	35.0	2.0	35.0
92-93	29.228912043822856	34.0	30.0	35.0	2.0	35.0
94-95	29.16311653207037	34.0	30.0	35.0	2.0	35.0
96-97	29.073391369097315	34.0	30.0	35.0	2.0	35.0
98-99	29.016182272539673	34.0	30.0	35.0	2.0	35.0
100-101	28.952856455489215	34.0	30.0	35.0	2.0	35.0
102-103	27.97465470658254	33.5	28.0	34.5	2.0	35.0
104-105	28.80336571148441	34.0	29.5	35.0	2.0	35.0
106-107	29.13944333328798	35.0	30.0	35.0	2.0	35.0
108-109	29.182738029711373	35.0	31.0	35.0	2.0	35.0
110-111	29.12681222610199	35.0	31.0	35.0	2.0	35.0
112-113	29.057739936612542	35.0	30.0	35.0	2.0	35.0
114-115	28.941545336214503	35.0	30.0	35.0	2.0	35.0
116-117	28.885568613190152	35.0	30.0	35.0	2.0	35.0
118-119	28.73299714150361	35.0	30.0	35.0	2.0	35.0
120-121	28.569818763989957	35.0	30.0	35.0	2.0	35.0
122-123	28.450183701581718	35.0	30.0	35.0	2.0	35.0
124-125	27.59557347424199	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80987.0
3	15233.0
4	10208.0
5	8842.0
6	9359.0
7	10944.0
8	12197.0
9	12773.0
10	13596.0
11	13849.0
12	14092.0
13	14689.0
14	16334.0
15	14028.0
16	10040.0
17	8953.0
18	8170.0
19	7535.0
20	7407.0
21	7831.0
22	9323.0
23	12657.0
24	18307.0
25	25071.0
26	29932.0
27	31790.0
28	37732.0
29	44592.0
30	50614.0
31	61229.0
32	77020.0
33	100567.0
34	142401.0
35	230267.0
36	437142.0
37	637439.0
38	182088.0
39	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25905997418948	17.224540366156297	12.534268638076576	24.98213102157764
2	16.757405029906337	19.308362102776506	38.333476991525266	25.60075587579189
3	18.79556203695608	24.21211391354699	28.91365421764858	28.078669831848345
4	13.421587830299611	15.279931072569712	35.50657660567051	35.79190449146017
5	14.713352625188078	36.837722395269644	33.16061129002455	15.288313689517727
6	33.521157436473246	34.29842747500881	17.504848914346436	14.675566174171518
7	29.85061944916953	30.974273349579196	20.895293374228473	18.2798138270228
8	27.812563801683233	32.82612327052326	19.76428178122957	19.597031146563936
9	27.953977611577113	14.272310267148278	19.028221026904546	38.74549109437007
10-11	26.42571001679726	24.524474608708385	26.895373380724497	22.154441993769854
12-13	26.771315624112578	22.381062466629974	26.88696745353768	23.960654455719766
14-15	24.326600593802013	23.51956273866587	24.270548851320793	27.88328781621133
16-17	24.478732294097608	26.288836258916366	24.849369388989118	24.38306205799691
18-19	24.566249488995513	25.420075597725717	26.196802421635322	23.81687249164345
20-21	25.893969954770846	24.48455554093253	25.67776691823313	23.943707586063496
22-23	25.58991175508416	24.07003736310611	25.470132823222634	24.8699180585871
24-25	24.9471378088957	24.613853297878705	25.639905848569676	24.799103044655915
26-27	24.61676969055707	24.60699324930544	25.744896759542858	25.03134030059463
28-29	24.6565402706262	24.601779767902094	25.780708421464055	24.96097154000765
30-31	25.022455931201833	24.544131705712314	25.545062943639994	24.888349419445856
32-33	24.66616597837497	24.622519050666945	25.538031923725963	25.17328304723212
34-35	25.004903229531983	24.699985254220337	25.605644230739482	24.689467285508197
36-37	25.118182817613683	24.417841722582505	25.406763611057197	25.05721184874662
38-39	25.074379698174067	24.636754988540073	25.387823368355	24.901041944930867
40-41	25.13133193163438	24.53583675611057	25.591854800818908	24.740976511436138
42-43	25.053620514039103	24.61001106032684	25.392464208642075	24.94390421699198
44-45	24.979341613190016	24.622445548700366	25.3524508499539	25.045761988155718
46-47	25.03545556507455	24.68854284486584	25.311962558098134	24.964039031961477
48-49	25.23041187133937	24.83551732608583	24.898094890605798	25.035975911969
50-51	25.092635887797805	25.073474465083823	24.869794110616066	24.964095536502303
52-53	25.28122057941382	24.778476652070346	25.07657087880761	24.86373188970823
54-55	25.208528840938104	24.76616431095042	25.145313283104638	24.879993565006835
56-57	25.00556862233933	24.906447144699207	24.966155150893165	25.12182908206829
58-59	25.147632314275743	24.643129095569545	24.96786656249768	25.241372027657032
60-61	24.965070945935494	24.93644716613238	25.182863264740064	24.915618623192067
62-63	24.92525243608895	25.035674856501384	25.084884399852907	24.954188307556755
64-65	25.01661786661447	24.67458783256615	25.180105088026743	25.128689212792633
66-67	24.929511937188494	24.91045665046005	25.104144153656556	25.055887258694902
68-69	25.07589795915842	24.863804355043662	25.008362076467527	25.05193560933039
70-71	24.905121930694456	24.645362277469676	25.26468918769522	25.184826604140643
72-73	25.16671131273675	24.906774262664484	25.03914522057118	24.88736920402759
74-75	24.927250491355647	25.103870418224606	24.947626540835625	25.021252549584126
76-77	25.01850310165395	24.789206933038464	25.003325299684253	25.18896466562333
78-79	25.027045820961817	24.866523769595588	24.978996440221415	25.127433969221176
80-81	24.926539999975255	25.007289294037545	24.914435854006268	25.151734851980933
82-83	25.14109878182777	24.90375715648609	24.871097167385127	25.084046894301014
84-85	24.865800637294907	24.95875150570922	24.910111066802973	25.2653367901929
86-87	24.71569809998041	24.963741894246333	24.971432695182425	25.34912731059083
88-89	24.812874560177892	24.828030174312243	25.00381467838756	25.3552805871223
90-91	24.770694219166575	24.83557836889866	25.046931218883906	25.346796193050857
92-93	24.904599768528016	25.030624778471662	24.91043502340597	25.154340429594352
94-95	24.708123912777683	24.759999249498307	25.154528092164906	25.377348745559104
96-97	24.92420893592315	24.735741548347935	25.03624610735775	25.303803408371163
98-99	24.737082243276216	24.791800722473607	24.95498714404382	25.51612989020636
100-101	24.69822770435335	24.93539010152645	25.091732139061325	25.274650055058874
102-103	24.860117744368218	24.58709479501941	25.265353649555884	25.28743381105649
104-105	24.640176938131646	24.85036106519955	25.109591591315468	25.399870405353337
106-107	24.659359255222952	24.93358304377066	24.962549619434864	25.444508081571527
108-109	24.790183131272187	24.753545330876243	25.025308328635916	25.43096320921566
110-111	24.78043355816387	24.928542977708542	25.03166704738594	25.25935641674164
112-113	24.82418002909441	24.869475113062467	25.10491883582769	25.201426022015433
114-115	24.47297504302234	25.086121993976974	25.17258932122323	25.26831364177745
116-117	24.563784180736448	25.18590997236142	25.027657136448333	25.2226487104538
118-119	24.721579859927132	24.7658046956856	25.018402904786406	25.49421253960086
120-121	24.322666315761595	24.888355910433134	25.254882049550442	25.534095724254826
122-123	24.472863491791163	24.928939779865914	25.18313575595207	25.415060972390858
124-125	24.448051305479368	25.1080890077829	25.153134684305055	25.290725002432673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	91.5
2	133.5
3	164.5
4	142.5
5	141.0
6	156.5
7	193.0
8	260.5
9	284.5
10	284.0
11	324.0
12	392.0
13	439.0
14	528.0
15	639.5
16	718.0
17	804.0
18	888.5
19	1031.5
20	1199.5
21	1400.5
22	1596.5
23	1871.0
24	2289.0
25	3048.5
26	4367.0
27	5883.0
28	7166.5
29	9028.5
30	11928.5
31	14776.5
32	18466.5
33	22114.0
34	25680.0
35	29659.0
36	34366.5
37	39316.0
38	42473.0
39	45707.0
40	49536.5
41	52997.5
42	56402.0
43	63458.0
44	70529.0
45	75375.0
46	81250.5
47	86306.5
48	92760.5
49	100268.0
50	100951.5
51	99456.0
52	99345.5
53	99797.0
54	101386.0
55	100395.5
56	95957.5
57	88137.5
58	84031.5
59	79983.0
60	71909.5
61	65074.0
62	57627.5
63	45747.0
64	34609.0
65	27681.0
66	21660.5
67	16910.5
68	13991.0
69	11813.5
70	10253.0
71	8428.0
72	6787.0
73	6496.5
74	5579.5
75	4389.5
76	3124.0
77	1898.0
78	1189.5
79	750.5
80	440.5
81	195.0
82	116.5
83	70.5
84	45.0
85	33.0
86	28.5
87	24.5
88	18.5
89	16.0
90	14.5
91	10.5
92	10.5
93	10.0
94	10.5
95	11.0
96	8.5
97	11.0
98	14.0
99	13.5
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03331407878532251
2	0.02737691622952246
3	0.028242752435576637
4	0.033891302922691956
5	0.03665373272296004
6	0.04729114896876846
7	0.04077676227559896
8	0.036942344791644766
9	0.031128873122423878
10-11	0.03704542053046073
12-13	0.03638573580203851
14-15	0.035272517822826
16-17	0.03822048395296283
18-19	0.05170279059009211
20-21	0.06275250979116442
22-23	0.05574335955167826
24-25	0.0678650664364367
26-27	0.04972373640482543
28-29	0.0498268121436414
30-31	0.0498268121436414
32-33	0.0579079500668137
34-35	0.04034384417257188
36-37	0.053455078149963656
38-39	0.06269066434787485
40-41	0.04947635463166709
42-43	0.0586294802385255
44-45	0.05961900733115885
46-47	0.06565924562577487
48-49	0.04986804243916779
50-51	0.051929557215487254
52-53	0.06196913417616303
54-55	0.04766222162850597
56-57	0.04547701596560734
58-59	0.04679638542245179
60-61	0.03477775427650933
62-63	0.04430195254310524
64-65	0.04339488604152468
66-67	0.036158969176643366
68-69	0.031499945782161386
70-71	0.0510018755661435
72-73	0.03197409418071486
74-75	0.040653071389019795
76-77	0.03329346363755931
78-79	0.03347899996742807
80-81	0.025232940862150218
82-83	0.01713118779121473
84-85	0.01913085712424461
86-87	0.017564105894241816
88-89	0.02296527460819881
90-91	0.012760776465417472
92-93	0.020017308478061976
94-95	0.014698600355157763
96-97	0.012905082499759832
98-99	0.010740491984624397
100-101	0.011111564644361901
102-103	0.006060853442379219
104-105	0.006040238294616024
106-107	0.007916216741066734
108-109	0.012781391613180666
110-111	0.006885459352907004
112-113	0.007957447036593124
114-115	0.008555286321725768
116-117	0.0069266896484333935
118-119	0.24515533719991048
120-121	0.36253798856354064
122-123	0.0287375159818933
124-125	0.003504575119743086
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2425401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.4459813390657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96450350351952	38.82549907509002
2	11.549010817926026	11.652023684115283
3	4.049388732414831	6.128261652900646
4	2.0284249756346897	4.093035538742494
5	1.1769245977815948	2.9685558148588873
6	0.7684926823724002	2.3260420508499986
7	0.5380668049565109	1.9000315601404798
8	0.41432026092966534	1.6720633721003784
9	0.3324839588569551	1.509523162563298
>10	1.992007247648269	18.437307646898322
>50	0.12763870468902808	4.412257327310535
>100	0.05543738447318777	4.7325042856289325
>500	0.0028052794776733826	0.9671306794572759
>1k	4.950493195892078E-4	0.3757641493431512
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1230295526389245E-5
2	0.0	0.0	0.0	0.0	4.1230295526389245E-5
3	0.0	0.0	0.0	0.0	4.1230295526389245E-5
4	0.0	0.0	0.0	0.0	4.1230295526389245E-5
5	0.0	0.0	0.0	0.0	4.1230295526389245E-5
6	0.0	0.0	0.0	0.0	1.2369088657916772E-4
7	0.0	0.0	0.0	0.0	1.2369088657916772E-4
8	0.0	0.0	0.0	0.0	1.6492118210555698E-4
9	0.0	0.0	0.0	0.0	2.4738177315833545E-4
10-11	0.0	0.0	0.0	0.0	2.4738177315833545E-4
12-13	0.0	0.0	0.0	0.0	3.0922721644791935E-4
14-15	0.0	0.0	0.0	0.0	3.916878075006978E-4
16-17	0.0	0.0	0.0	0.0	4.329181030270871E-4
18-19	0.0	0.0	0.0	2.0615147763194623E-5	4.947635463166709E-4
20-21	0.0	0.0	0.0	4.1230295526389245E-5	4.947635463166709E-4
22-23	0.0	0.0	0.0	4.1230295526389245E-5	5.359938418430602E-4
24-25	0.0	0.0	0.0	1.4430603434236237E-4	5.772241373694495E-4
26-27	0.0	0.0	0.0	2.473817731583355E-4	5.772241373694495E-4
28-29	0.0	0.0	0.0	7.833756150013956E-4	5.97839285132644E-4
30-31	0.0	0.0	0.0	0.003154117607768777	6.390695806590334E-4
32-33	0.0	0.0	0.0	0.008514056026199378	7.421453194750064E-4
34-35	0.0	0.0	0.0	0.015997354664239027	7.833756150013956E-4
36-37	0.0	0.0	0.0	0.02702645871754815	7.833756150013956E-4
38-39	0.0	0.0	0.0	0.04430195254310525	8.039907627645903E-4
40-41	0.0	0.0	0.0	0.06473156397643111	9.482967971069527E-4
42-43	0.0	0.0	0.0	0.08584147528594241	9.482967971069527E-4
44-45	0.0	0.0	0.0	0.11002304361216969	9.482967971069527E-4
46-47	0.0	0.0	0.0	0.14049223200617134	0.0010101422403965363
48-49	0.0	0.0	0.0	0.1721570989704383	0.0010307573881597311
50-51	0.0	0.0	0.0	0.2066050108827365	0.0010307573881597311
52-53	0.0	0.0	0.0	0.2411559985338507	0.0011132179792125096
54-55	0.0	4.1230295526389245E-5	0.0	0.2801186278062885	0.001257524013554872
56-57	0.0	4.1230295526389245E-5	0.0	0.3246679621225521	0.001360599752370845
58-59	0.0	4.1230295526389245E-5	0.0	0.371691114170399	0.0014018300478972343
60-61	0.0	4.1230295526389245E-5	0.0	0.42275483517983214	0.0014018300478972343
62-63	0.0	4.1230295526389245E-5	0.0	0.4764366799551909	0.0015255209344764021
64-65	0.0	4.1230295526389245E-5	0.0	0.5364061447983242	0.0015255209344764021
66-67	0.0	4.1230295526389245E-5	0.0	0.60439490212134	0.0015255209344764021
68-69	0.0	4.1230295526389245E-5	0.0	0.6807534094362129	0.0015255209344764021
70-71	0.0	4.1230295526389245E-5	0.0	0.7670484179729455	0.0016079815255291806
72-73	0.0	4.1230295526389245E-5	0.0	0.8598784283506109	0.001855363298687516
74-75	0.0	4.1230295526389245E-5	0.0	0.9537391961164361	0.001855363298687516
76-77	0.0	4.1230295526389245E-5	0.0	1.061164731110443	0.0018759784464507105
78-79	0.0	4.1230295526389245E-5	0.0	1.1819076515594742	0.0018965935942139053
80-81	0.0	4.1230295526389245E-5	0.0	1.3160091877590552	0.0019996693330298783
82-83	0.0	4.1230295526389245E-5	0.0	1.4592638495654944	0.0022470511061882135
84-85	0.0	4.1230295526389245E-5	0.0	1.611197488580239	0.0022676662539514083
86-87	0.0	4.1230295526389245E-5	0.0	1.7723460986451314	0.002308896549477798
88-89	0.0	4.1230295526389245E-5	0.0	1.9538006292567704	0.002350126845004187
90-91	0.0	4.1230295526389245E-5	0.0	2.155272468346471	0.002350126845004187
92-93	0.0	4.1230295526389245E-5	0.0	2.375236094979758	0.002556278322636133
94-95	0.0	8.246059105277849E-5	0.0	2.6090118706143848	0.0026593540614521065
96-97	0.0	8.246059105277849E-5	0.0	2.8500029479661304	0.0028036600957944686
98-99	0.0	8.246059105277849E-5	0.0	3.113196539458836	0.0028448903913208578
100-101	0.0	8.246059105277849E-5	0.0	3.4144250785746353	0.0028448903913208578
102-103	0.0	8.246059105277849E-5	0.0	3.7390724255494243	0.0029685812779000256
104-105	0.0	8.246059105277849E-5	0.0	4.076295012659762	0.0030098115734264147
106-107	0.0	8.246059105277849E-5	0.0	4.42732974877144	0.0030304267211896095
108-109	0.0	8.246059105277849E-5	0.0	4.796773811835651	0.0031747327555319716
110-111	0.0	8.246059105277849E-5	0.0	5.204376513409535	0.0032778084943479446
112-113	0.0	8.246059105277849E-5	0.0	5.639747819020442	0.0032984236421111394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2580	0.0	75.6664	1
GTATCAA	5325	0.0	60.80344	1
TCAACGC	6855	0.0	46.17339	4
CAACGCA	7110	0.0	44.35003	5
AACGCAG	7530	0.0	41.713993	6
ATCAACG	7635	0.0	41.608685	3
TATCAAC	8305	0.0	38.824993	2
GTACATG	12945	0.0	37.05799	1
ACGCAGA	8735	0.0	36.02762	7
TACATGG	13475	0.0	35.31937	2
ACATGGG	13715	0.0	34.441055	3
CGCAGAG	9270	0.0	33.81931	8
CATGGGG	11065	0.0	32.369255	4
GCAGAGT	10005	0.0	30.383492	9
AGAGTAC	9205	0.0	26.044178	10-11
ATGGGGG	5435	0.0	25.834501	5
CAGAGTA	9670	0.0	25.806843	10-11
ATGGGGA	4415	0.0	24.25657	5
CATGGGT	2650	0.0	22.002285	4
GTATAGG	825	0.0	21.642937	1
>>END_MODULE