FastQCFastQC Report
Mon 15 Aug 2016
SRR2921551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425401
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.11449653067678295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.10146775729044392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.084.0454251
GTATCAA68750.062.0131341
TCAACGC94100.044.675924
CAACGCA96050.043.741835
ATCAACG99750.042.2050973
AACGCAG100400.041.7778856
TATCAAC103050.041.201112
GTACATG134950.036.6295851
ACGCAGA118300.035.3529667
TACATGG142800.034.2359432
ACATGGG142800.033.859943
CGCAGAG124550.033.576168
CATGGGG113000.030.985414
GCAGAGT133750.030.866349
GTATAGG5850.027.5206221
CAGAGTA130250.026.74127210-11
AGAGTAC127450.025.90514410-11
ATGGGGA46350.025.164945
GAGTACT65450.024.67932912-13
ATGGGTA19250.024.4222665
Produced by FastQC (version 0.11.5)