FastQCFastQC Report
Mon 15 Aug 2016
SRR2921550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281762
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.075.446731
GTATCAA49450.058.9640661
TCAACGC66200.043.3153574
CAACGCA67350.042.4874155
ATCAACG71850.040.0730483
AACGCAG72250.039.604186
TATCAAC74800.038.6499982
GTACATG121800.038.1558721
TACATGG125650.036.3117872
ACATGGG128250.034.97423
ACGCAGA84350.033.7819147
CATGGGG110150.032.5675964
CGCAGAG91650.031.0911548
ATGGGGG57450.028.5806355
GCAGAGT97900.028.255579
GTATACG1704.424237E-728.0026231
AGAGTAC88400.026.27862210-11
CAGAGTA92750.024.88580110-11
ATGGGGA41500.024.2265455
GTATCGA2055.9490038E-520.3131839
Produced by FastQC (version 0.11.5)