FastQCFastQC Report
Mon 15 Aug 2016
SRR2921550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281762
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.1386209429379576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.11670805281181824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.082.3992841
GTATCAA64650.061.8882261
TCAACGC90300.043.649174
CAACGCA91650.043.1096735
AACGCAG95700.041.3393066
ATCAACG96450.040.9903033
TATCAAC98050.040.5035932
ACGCAGA111650.035.2205547
GTACATG126050.034.4856761
CGCAGAG116900.033.6889468
TACATGG130700.033.2098772
ACATGGG132350.032.1660273
GCAGAGT125800.030.5475799
CATGGGG112300.029.425174
AGAGTAC117650.028.44200910-11
CAGAGTA122800.026.0865710-11
ATGGGGG58550.024.7971655
GAGTACT62150.023.21108812-13
AGTACTT66700.023.14389212-13
GCGCGAC3609.458745E-1121.4815569
Produced by FastQC (version 0.11.5)