FastQCFastQC Report
Mon 15 Aug 2016
SRR2921549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709934
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.065.858291
GTATCAA52200.058.601061
TCAACGC67150.045.00934
CAACGCA68150.043.999655
AACGCAG71300.042.1376536
ATCAACG72650.041.6806683
TATCAAC73350.041.2828982
GTACATG137000.037.3586121
TACATGG140950.035.8339842
ACGCAGA84150.035.490997
ACATGGG142950.034.9580843
CGCAGAG90850.032.9372638
CATGGGG131500.030.9919834
GCAGAGT101250.028.9670589
ATGGGGG66300.026.2929125
AGAGTAC86550.026.22261210-11
GTATATA7150.025.80291
CAGAGTA93600.024.94665310-11
ATGGGGA54550.023.0129475
ATAGGAC7450.020.7620183
Produced by FastQC (version 0.11.5)