FastQCFastQC Report
Mon 15 Aug 2016
SRR2921549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709934
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.072.850211
GTATCAA60600.061.2767941
TCAACGC81400.044.6889234
CAACGCA83600.043.3472445
AACGCAG86600.041.837896
ATCAACG88400.041.4203643
TATCAAC89550.041.1551482
GTACATG129700.037.959521
TACATGG135300.036.172052
ACGCAGA103350.035.171037
ACATGGG138950.034.8784333
CGCAGAG112650.032.266838
CATGGGG125100.032.266774
GCAGAGT121550.029.5121369
ATGGGGG65000.029.0199345
AGAGTAC108400.027.38530510-11
CAGAGTA116200.024.83029710-11
GTATAGG7950.023.242091
TACCGTA3103.3833203E-1023.0289367
ACCGTAT3557.8216544E-1121.7851878
Produced by FastQC (version 0.11.5)