##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2709934
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38616770740542	33.0	31.0	34.0	28.0	34.0
2	31.458249536704585	33.0	31.0	34.0	28.0	34.0
3	31.542559339083535	34.0	31.0	34.0	28.0	34.0
4	35.05426220712386	37.0	35.0	37.0	32.0	37.0
5	34.712138376801796	37.0	35.0	37.0	32.0	37.0
6	34.69049725934285	37.0	35.0	37.0	31.0	37.0
7	34.54214604488523	37.0	35.0	37.0	31.0	37.0
8	34.51847683375315	37.0	35.0	37.0	31.0	37.0
9	36.137279727107746	39.0	37.0	39.0	31.0	39.0
10-11	35.99714365737321	39.0	37.0	39.0	30.0	39.0
12-13	35.90885589833553	39.0	37.0	39.0	30.0	39.0
14-15	37.11652202599768	40.0	37.0	41.0	31.0	41.0
16-17	37.088855300534995	40.0	37.0	41.0	31.0	41.0
18-19	37.08467881505601	40.0	37.0	41.0	30.5	41.0
20-21	37.05492569191722	40.0	37.0	41.0	30.0	41.0
22-23	36.98611331493682	40.0	37.0	41.0	30.0	41.0
24-25	36.912963378443905	40.0	37.0	41.0	30.0	41.0
26-27	36.790516853916	40.0	37.0	41.0	30.0	41.0
28-29	36.608902467735376	40.0	36.0	41.0	30.0	41.0
30-31	36.579284587742734	40.0	36.0	41.0	30.0	41.0
32-33	36.48300751974033	40.0	36.0	41.0	30.0	41.0
34-35	36.373654303019926	40.0	36.0	41.0	29.5	41.0
36-37	36.25309583185421	40.0	35.5	41.0	29.0	41.0
38-39	36.09480193982584	40.0	35.0	41.0	28.5	41.0
40-41	35.90901752588809	40.0	35.0	41.0	27.5	41.0
42-43	35.751437857896164	40.0	35.0	41.0	27.0	41.0
44-45	35.5642130029735	39.0	35.0	41.0	26.5	41.0
46-47	35.32769248254755	39.0	35.0	41.0	26.0	41.0
48-49	35.11235624188633	39.0	35.0	41.0	25.0	41.0
50-51	34.900465472590845	39.0	34.5	41.0	24.0	41.0
52-53	34.693518919648966	38.5	34.0	41.0	24.0	41.0
54-55	34.46371664402159	38.0	34.0	40.5	23.5	41.0
56-57	34.195449593975354	38.0	34.0	40.0	23.0	41.0
58-59	34.03592209256757	37.0	34.0	40.0	23.0	41.0
60-61	33.90614808331125	37.0	34.0	40.0	23.0	41.0
62-63	33.62424195570814	36.5	33.5	40.0	23.0	41.0
64-65	33.31783320184182	36.0	33.0	39.0	22.0	41.0
66-67	33.00969949821656	35.0	33.0	39.0	22.0	41.0
68-69	32.68482534999008	35.0	33.0	38.5	22.0	40.5
70-71	32.38061867927411	35.0	33.0	37.0	21.5	40.0
72-73	32.06755090714387	35.0	33.0	37.0	20.5	39.0
74-75	31.78533019623356	35.0	32.0	36.5	20.0	39.0
76-77	31.500764040747853	35.0	32.0	36.0	20.0	38.5
78-79	31.23291323700134	35.0	32.0	35.5	19.0	37.0
80-81	31.021429673194994	35.0	32.0	35.0	18.5	37.0
82-83	30.84815589604765	35.0	32.0	35.0	18.0	36.5
84-85	30.67153738799543	35.0	32.0	35.0	18.0	36.0
86-87	30.534432572896613	35.0	32.0	35.0	17.0	36.0
88-89	30.441936962302403	35.0	32.0	35.0	17.0	35.5
90-91	30.35562545065673	35.0	31.0	35.0	15.0	35.0
92-93	30.429922463056293	35.0	32.0	35.0	17.0	35.0
94-95	30.40723113551843	35.0	32.0	35.0	15.5	35.0
96-97	30.39287119169692	35.0	32.0	35.0	15.0	35.0
98-99	30.354649043113227	35.0	32.0	35.0	14.0	35.0
100-101	30.310206447832307	35.0	32.0	35.0	10.5	35.0
102-103	29.42195640189023	34.0	30.0	35.0	10.0	35.0
104-105	30.138730500447615	35.0	31.5	35.0	10.0	35.0
106-107	30.25044060113641	35.0	32.0	35.0	7.5	35.0
108-109	30.25773561274924	35.0	32.0	35.0	7.0	35.0
110-111	30.229068309412703	35.0	32.0	35.0	6.0	35.0
112-113	30.184045810709783	35.0	32.0	35.0	2.0	35.0
114-115	30.121681930261033	35.0	32.0	35.0	2.0	35.0
116-117	30.045629524556688	35.0	32.0	35.0	2.0	35.0
118-119	29.95187576524004	35.0	32.0	35.0	2.0	35.0
120-121	29.829946042966363	35.0	32.0	35.0	2.0	35.0
122-123	29.713581585381785	35.0	32.0	35.0	2.0	35.0
124-125	28.781961479504666	34.0	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42063.0
3	16048.0
4	9251.0
5	7622.0
6	7848.0
7	9067.0
8	10496.0
9	11649.0
10	13018.0
11	13632.0
12	13920.0
13	15230.0
14	18308.0
15	15105.0
16	9418.0
17	8383.0
18	7606.0
19	6937.0
20	7053.0
21	7450.0
22	9096.0
23	12070.0
24	16533.0
25	23060.0
26	28878.0
27	33226.0
28	39385.0
29	46763.0
30	54968.0
31	66641.0
32	83430.0
33	108157.0
34	150670.0
35	244023.0
36	504029.0
37	803434.0
38	235154.0
39	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03124958434876	17.130779769126978	12.320087667313482	25.51788297921079
2	16.989102164806326	19.380005727157055	37.37209841263281	26.25879369540381
3	19.062760672340865	23.796053651442787	28.742350535554785	28.398835140661564
4	13.771418845149997	15.320587161301733	34.67850825259441	36.22948574095386
5	14.930524155521063	36.658515474039525	33.06256609386852	15.34839427657089
6	34.46433949673129	34.075423868944625	16.700709548640845	14.759527085683239
7	30.225109698211355	30.786582955616193	20.39912470257197	18.589182643600484
8	28.34098772038269	33.01074812484431	19.108745191021395	19.539518963751604
9	28.121867873142865	14.015432759371471	18.355905558302766	39.5067938091829
10-11	26.855541423275405	24.194080225056922	26.62776834976526	22.322610001902415
12-13	27.216882746893123	21.835979534990827	26.89237492416403	24.054762793952015
14-15	24.64288923491197	22.877064444984452	24.159539396905018	28.320506923198558
16-17	24.79661917377387	25.860287009294797	24.60126065019494	24.741833166736388
18-19	24.871515397763012	25.01263488046268	26.175191497551165	23.94065822422314
20-21	25.909932459401553	24.002460920250574	25.8816621397864	24.205944480561477
22-23	25.808312340524154	23.63154316891652	25.72710282995123	24.83304166060809
24-25	25.132726313819813	24.149029183365485	25.72251647245659	24.995728030358112
26-27	24.9149014181344	24.218538804713447	25.873936186996744	24.992623590155414
28-29	25.09700980247424	24.25599368153769	25.798259470312086	24.848737045675986
30-31	25.206283425149167	24.19949887766979	25.663981551080095	24.93023614610095
32-33	24.93151514066684	24.264907647625122	25.626799082722695	25.17677812898534
34-35	25.193351241899254	24.265091805391613	25.717985116549624	24.823571836159505
36-37	25.297860922698987	24.174806586931986	25.590850072670612	24.936482417698414
38-39	25.329564873972533	24.308352987639008	25.4661669121148	24.895915226273654
40-41	25.325495869369856	24.23909493345045	25.49431934559452	24.941089851585176
42-43	25.220355909369125	24.207347036084542	25.445034331918453	25.12726272262788
44-45	25.186534112047433	24.243123261623133	25.303016434489393	25.267326191840038
46-47	25.33983178601093	24.395586509590174	25.096278214282485	25.168303490116408
48-49	25.428027854267988	24.48208781886052	24.84524227613949	25.244642050732004
50-51	25.325966606138444	24.73528130859058	24.7526831716787	25.186068913592273
52-53	25.478226094139483	24.57301389892886	24.81748578373936	25.13127422319229
54-55	25.330375501445513	24.516399653504795	24.908310533225414	25.244914311824278
56-57	25.278094789556672	24.589621485475366	24.807738302634455	25.32454542233351
58-59	25.32087764131682	24.46129168936505	24.924966155417863	25.29286451390027
60-61	25.33037346854864	24.52427844529959	25.0513590304419	25.093989055709876
62-63	25.192563612845316	24.651538155896016	24.938863348256625	25.217034883002043
64-65	25.26127752080467	24.400652905456965	25.00057669009412	25.337492883644234
66-67	25.257133662264636	24.618271520008797	24.925735805103592	25.198859012622975
68-69	25.271984565487237	24.562305550644403	24.804937006621383	25.360772877246973
70-71	25.15477059554293	24.384294632134264	25.07617658500074	25.38475818732206
72-73	25.334653425149224	24.604272336294926	24.800902042236174	25.260172196319676
74-75	25.184413931676236	24.784804277266428	24.798571193410776	25.23221059764656
76-77	25.280351912002807	24.46253811992511	24.795454285430633	25.46165568264145
78-79	25.156856147847407	24.51926209183488	24.8501849605984	25.473696799719313
80-81	25.1301620039892	24.717087264829942	24.687393423284696	25.465357307896163
82-83	25.206023222221646	24.616684245838755	24.72743672051626	25.44985581142334
84-85	25.167707273235575	24.630534690627528	24.650740942017286	25.55101709411961
86-87	24.98508059798661	24.68145185249703	24.763753068303735	25.569714481212625
88-89	25.051036471284306	24.5560629777691	24.75413726679258	25.63876328415402
90-91	24.964745029335162	24.53555889600734	24.780618309997944	25.719077764659552
92-93	24.964438004217804	24.627205660248194	24.788274883848036	25.620081451685966
94-95	24.95978808036959	24.416721884824057	24.89181023848893	25.731679796317426
96-97	24.935886755501727	24.499866874722194	24.840973875525005	25.72327249425107
98-99	24.884487547608177	24.53923253456517	24.741861358487697	25.83441855933895
100-101	24.825767512376377	24.52219849586544	24.857598802479185	25.794435189279003
102-103	24.828670380299908	24.335503822188436	24.97598562274087	25.85984017477079
104-105	24.711096432192466	24.57151344674028	24.82305803902973	25.894332082037526
106-107	24.758872700468924	24.47635591727085	24.81557163429649	25.949199747963736
108-109	24.7311421165932	24.44974906597549	24.825536493161252	25.99357232427006
110-111	24.702536466128787	24.529578820271656	24.839644239370134	25.92824047422942
112-113	24.696382796083054	24.469048169107438	24.86915342208733	25.965415612722175
114-115	24.535308783023797	24.625903735390413	24.877410822092916	25.961376659492874
116-117	24.508252304915953	24.608798583605733	24.846611106959358	26.036338004518957
118-119	24.5311616871481	24.422291517777484	24.85841803524773	26.188128759826686
120-121	24.367715605887778	24.442037967877535	25.014253730048928	26.17599269618576
122-123	24.315249020400504	24.512783365194803	24.89750115179166	26.274466462613038
124-125	24.28515667173265	24.55192816455492	24.889512678335958	26.273402485376472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	20.5
2	37.5
3	53.5
4	51.0
5	53.5
6	83.5
7	168.5
8	272.0
9	319.0
10	304.0
11	295.0
12	357.5
13	450.0
14	549.5
15	662.5
16	806.5
17	948.0
18	1101.0
19	1291.0
20	1511.5
21	1700.5
22	1916.0
23	2260.5
24	2626.5
25	3144.0
26	4038.0
27	5100.0
28	6180.5
29	7766.0
30	9939.5
31	12079.0
32	14690.0
33	17521.0
34	20810.5
35	24808.5
36	29680.0
37	34942.0
38	39532.0
39	45211.0
40	51347.5
41	56715.0
42	62237.5
43	70214.0
44	78292.0
45	84763.0
46	92789.0
47	100337.5
48	108115.0
49	115090.0
50	116971.5
51	118099.5
52	118993.0
53	120230.0
54	123106.5
55	123089.0
56	117867.0
57	108484.0
58	101197.0
59	95104.0
60	85521.0
61	74907.5
62	63672.0
63	50127.0
64	38099.0
65	30079.0
66	23437.5
67	18223.0
68	14667.5
69	11741.0
70	9689.5
71	7796.5
72	6236.0
73	5515.5
74	4464.5
75	3426.5
76	2479.0
77	1524.5
78	882.5
79	494.0
80	278.5
81	144.0
82	85.5
83	38.5
84	25.0
85	14.0
86	10.5
87	8.5
88	2.0
89	4.0
90	4.5
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12317643160313128
2	0.0014022481728337296
3	0.021919353017453562
4	0.055831618039406124
5	0.02653201148072241
6	0.02236216822992737
7	0.016790076806298602
8	0.005867301565277974
9	0.004206744518501188
10-11	0.008099828261500096
12-13	0.005092374943448807
14-15	0.0035240710659373994
16-17	0.011088830945698309
18-19	0.00640236994701716
20-21	0.013967129826778068
22-23	0.010184749886897614
24-25	0.015166421027227968
26-27	0.010424608126987596
28-29	0.015277124830346421
30-31	0.022712730273135803
32-33	0.01452064884237033
34-35	0.022731180906988877
36-37	0.016531767932355546
38-39	0.02228836569451507
40-41	0.011033479044139082
42-43	0.010461509394693745
44-45	0.018819646530136895
46-47	0.01728824392033164
48-49	0.022823434076254253
50-51	0.016753175538592454
52-53	0.02304484168249116
54-55	0.01907795540407995
56-57	0.02236216822992737
58-59	0.018081621176013883
60-61	0.02125513019874285
62-63	0.02341385435955267
64-65	0.018690492093165367
66-67	0.012952344964858923
68-69	0.02512976330788868
70-71	0.016236557790706343
72-73	0.019428517447288386
74-75	0.01992668456132142
76-77	0.014502198208517255
78-79	0.031126219310138183
80-81	0.02280498344240118
82-83	0.010627565099371425
84-85	0.013893327291365767
86-87	0.013837975389806542
88-89	0.022030056820572016
90-91	0.01428079060228035
92-93	0.008468840938561603
94-95	0.008395038403149301
96-97	0.0029705520503451378
98-99	0.001955767188425991
100-101	0.012398825949266662
102-103	0.003653225502908927
104-105	0.002822946979520535
106-107	2.3985824008998004E-4
108-109	0.020535555478472908
110-111	8.118278895353171E-4
112-113	0.01003714481607301
114-115	0.0025830887394305544
116-117	0.008727149812504659
118-119	0.010498410662399897
120-121	0.004317448321619641
122-123	9.409823265068448E-4
124-125	0.02533272028027251
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2709934.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.94212625923647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87381457665843	38.6517344193403
2	11.89679248654278	12.12095809858796
3	4.342406558551012	6.636342695239265
4	2.1612900328221145	4.404028389394139
5	1.2807661604957787	3.2622475728266735
6	0.8596774476633431	2.62762782486505
7	0.5881038869308478	2.0971483723105284
8	0.45163353040789994	1.840573786315516
9	0.3470626334128391	1.5912097642061955
>10	2.036190860771399	18.97500862459036
>50	0.12295961935243907	4.233936844931773
>100	0.038647020436407574	3.2143665994019
>500	3.639921970571452E-4	0.10906719945587735
>1k	2.911937576256117E-4	0.23574980853440855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.690126770615078E-5
2	0.0	0.0	0.0	0.0	3.690126770615078E-5
3	0.0	0.0	0.0	0.0	3.690126770615078E-5
4	0.0	0.0	0.0	0.0	3.690126770615078E-5
5	0.0	0.0	0.0	0.0	3.690126770615078E-5
6	0.0	0.0	0.0	0.0	3.690126770615078E-5
7	0.0	0.0	0.0	0.0	3.690126770615078E-5
8	0.0	0.0	0.0	0.0	3.690126770615078E-5
9	0.0	0.0	0.0	0.0	7.380253541230155E-5
10-11	0.0	0.0	0.0	0.0	7.380253541230155E-5
12-13	0.0	0.0	0.0	0.0	1.476050708246031E-4
14-15	0.0	0.0	0.0	0.0	2.5830887394305546E-4
16-17	0.0	0.0	0.0	1.845063385307539E-5	3.32111409355357E-4
18-19	0.0	0.0	0.0	3.690126770615078E-5	3.505620432084324E-4
20-21	0.0	0.0	0.0	3.690126770615078E-5	3.8746331091458314E-4
22-23	0.0	0.0	0.0	7.380253541230155E-5	4.981671140330355E-4
24-25	0.0	0.0	0.0	2.0295697238382929E-4	5.535190155922617E-4
26-27	0.0	0.0	0.0	2.5830887394305546E-4	6.088709171514879E-4
28-29	0.0	0.0	0.0	0.0012177418343029757	6.64222818710714E-4
30-31	0.0	0.0	0.0	0.004391250857031943	6.64222818710714E-4
32-33	0.0	0.0	0.0	0.010627565099371423	6.64222818710714E-4
34-35	0.0	0.0	0.0	0.018782745262430747	6.64222818710714E-4
36-37	0.0	0.0	0.0	0.030701854731517448	6.64222818710714E-4
38-39	0.0	0.0	0.0	0.050536286123573484	7.011240864168648E-4
40-41	0.0	0.0	0.0	0.07326746703056236	7.195747202699402E-4
42-43	0.0	0.0	0.0	0.09548203018966514	7.380253541230155E-4
44-45	0.0	0.0	0.0	0.12221699864277137	7.564759879760909E-4
46-47	0.0	0.0	0.0	0.15319561288208494	7.933772556822417E-4
48-49	0.0	0.0	0.0	0.18651745762073912	8.487291572414679E-4
50-51	0.0	0.0	0.0	0.22140760623690467	8.856304249476186E-4
52-53	0.0	0.0	0.0	0.2567590206993971	0.0010332354957722216
54-55	0.0	0.0	0.0	0.2956898581293862	0.001236192468156051
56-57	0.0	0.0	0.0	0.34104151614024547	0.0013099950035683526
58-59	0.0	0.0	0.0	0.38971428824465837	0.0013468962712745035
60-61	0.0	0.0	0.0	0.441025500990061	0.0014206988066868048
62-63	0.0	0.0	0.0	0.49624824811231566	0.0015498532436583326
64-65	0.0	0.0	0.0	0.5542201396786786	0.001568303877511408
66-67	0.0	0.0	0.0	0.6207715759867214	0.0015867545113644834
68-69	0.0	0.0	0.0	0.6961424152765344	0.0015867545113644834
70-71	0.0	0.0	0.0	0.7787828043044591	0.0016052051452175589
72-73	0.0	0.0	0.0	0.8671428898268372	0.0018081621176013881
74-75	0.0	0.0	0.0	0.9607798566311947	0.0018081621176013881
76-77	0.0	0.0	0.0	1.060929897185688	0.0018635140191606142
78-79	0.0	0.0	0.0	1.1743090422128362	0.0018819646530136896
80-81	0.0	0.0	0.0	1.3021719348146485	0.0019188659207198403
82-83	0.0	0.0	0.0	1.4377471923670466	0.002103372259250594
84-85	0.0	0.0	0.0	1.5774184906348272	0.00215872416080982
86-87	0.0	0.0	0.0	1.7300974857690261	0.0021956254285159715
88-89	0.0	0.0	0.0	1.9021680970828072	0.0022140760623690467
90-91	0.0	0.0	0.0	2.09416539295791	0.0022140760623690467
92-93	0.0	0.0	0.0	2.3026944567653675	0.002287878597781348
94-95	0.0	0.0	0.0	2.518363915873966	0.002324779865487499
96-97	0.0	0.0	0.0	2.7419486969055336	0.002472384936312102
98-99	0.0	0.0	0.0	2.984611433341181	0.002564638105577479
100-101	0.0	0.0	0.0	3.2651348704433394	0.0025830887394305544
102-103	0.0	0.0	0.0	3.567374703590567	0.002693792542549007
104-105	0.0	0.0	0.0	3.8779911245070915	0.0027306938102551574
106-107	0.0	0.0	0.0	4.202999039829014	0.0027306938102551574
108-109	0.0	0.0	0.0	4.543726895193757	0.002859848247226685
110-111	0.0	0.0	0.0	4.921189962559973	0.003099706487316665
112-113	0.0	0.0	0.0	5.32942868719312	0.003173509022728967
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3060	0.0	72.85021	1
GTATCAA	6060	0.0	61.276794	1
TCAACGC	8140	0.0	44.688923	4
CAACGCA	8360	0.0	43.347244	5
AACGCAG	8660	0.0	41.83789	6
ATCAACG	8840	0.0	41.420364	3
TATCAAC	8955	0.0	41.155148	2
GTACATG	12970	0.0	37.95952	1
TACATGG	13530	0.0	36.17205	2
ACGCAGA	10335	0.0	35.17103	7
ACATGGG	13895	0.0	34.878433	3
CGCAGAG	11265	0.0	32.26683	8
CATGGGG	12510	0.0	32.26677	4
GCAGAGT	12155	0.0	29.512136	9
ATGGGGG	6500	0.0	29.019934	5
AGAGTAC	10840	0.0	27.385305	10-11
CAGAGTA	11620	0.0	24.830297	10-11
GTATAGG	795	0.0	23.24209	1
TACCGTA	310	3.3833203E-10	23.028936	7
ACCGTAT	355	7.8216544E-11	21.785187	8
>>END_MODULE