FastQCFastQC Report
Mon 15 Aug 2016
SRR2921548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194862
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.10592921103923618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.072.612381
GTATCAA55400.060.7906651
TCAACGC69850.047.5289654
CAACGCA70400.047.242165
AACGCAG74050.044.6684386
ATCAACG74600.044.497593
TATCAAC80000.041.7914472
ACGCAGA85500.038.6169437
GTACATG116450.037.504791
CGCAGAG89600.036.4514968
TACATGG122300.035.3143542
ACATGGG121900.035.0398183
GCAGAGT96600.033.31749
CATGGGG94900.032.5380674
ATGGGGG51500.028.4196345
AGAGTAC88250.027.8073910-11
CAGAGTA94350.026.92383810-11
GAGTACT37850.022.47764612-13
GTATAGG7500.022.2140081
GTGGTAT12750.021.467321
Produced by FastQC (version 0.11.5)