##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921548_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194862
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.939448584922424	31.0	30.0	34.0	26.0	34.0
2	29.958005560258457	31.0	30.0	34.0	25.0	34.0
3	30.111817508344487	31.0	30.0	34.0	26.0	34.0
4	33.47511187491514	37.0	35.0	37.0	27.0	37.0
5	33.53176190575991	37.0	35.0	37.0	27.0	37.0
6	33.52498425869143	37.0	35.0	37.0	28.0	37.0
7	33.38220717293388	37.0	35.0	37.0	27.0	37.0
8	33.3332004472263	37.0	35.0	37.0	26.0	37.0
9	34.789635977113825	39.0	35.0	39.0	27.0	39.0
10-11	34.667245138874335	39.0	35.0	39.0	26.5	39.0
12-13	34.51722773459106	39.0	35.0	39.0	25.5	39.0
14-15	35.66287493245589	40.0	36.0	41.0	25.0	41.0
16-17	35.59295231317504	40.0	36.0	41.0	25.0	41.0
18-19	35.61573962281	40.0	36.0	41.0	25.0	41.0
20-21	35.64845603049304	40.0	36.0	41.0	25.0	41.0
22-23	35.61447963471052	40.0	36.0	41.0	25.0	41.0
24-25	35.57940909269011	40.0	35.5	41.0	25.0	41.0
26-27	35.50246097476743	40.0	35.0	41.0	25.0	41.0
28-29	35.46338995344583	40.0	35.0	41.0	25.0	41.0
30-31	35.364398308413016	40.0	35.0	41.0	25.0	41.0
32-33	35.25220628905143	40.0	35.0	41.0	25.0	41.0
34-35	35.08003168308531	40.0	35.0	41.0	24.0	41.0
36-37	34.935670215257275	39.5	35.0	41.0	24.0	41.0
38-39	34.80131438787495	39.0	34.5	41.0	23.0	41.0
40-41	34.65520201270057	39.0	34.0	41.0	22.5	41.0
42-43	34.446993478405474	39.0	34.0	41.0	20.5	41.0
44-45	34.244265470904324	39.0	33.5	41.0	19.0	41.0
46-47	33.98339736165645	39.0	33.0	41.0	16.5	41.0
48-49	33.8199506392657	38.0	33.0	41.0	15.0	41.0
50-51	33.62198078968063	38.0	33.0	40.5	13.0	41.0
52-53	33.442636029053304	38.0	33.0	40.0	12.5	41.0
54-55	33.245301299124954	38.0	33.0	40.0	10.0	41.0
56-57	33.01122963539393	37.5	32.5	40.0	8.0	41.0
58-59	32.77745480125857	37.0	32.0	40.0	7.5	41.0
60-61	32.48274287859556	36.5	32.0	40.0	7.0	41.0
62-63	32.17345486868878	36.0	32.0	39.5	4.0	41.0
64-65	31.875234069385684	35.5	31.0	39.0	2.0	41.0
66-67	31.839149112791603	35.0	32.0	39.0	2.0	41.0
68-69	31.593222489614384	35.0	31.0	39.0	2.0	40.5
70-71	31.29785016096684	35.0	31.0	37.5	2.0	40.0
72-73	30.973144553051625	35.0	31.0	37.0	2.0	39.5
74-75	30.668134033028046	35.0	31.0	36.5	2.0	39.0
76-77	30.38976368445943	35.0	31.0	36.0	2.0	39.0
78-79	30.107419509745945	35.0	31.0	36.0	2.0	37.5
80-81	29.861817280539732	35.0	30.0	35.0	2.0	37.0
82-83	29.65850768749926	35.0	30.0	35.0	2.0	37.0
84-85	29.453124615579476	35.0	30.0	35.0	2.0	36.0
86-87	29.29589149568401	35.0	30.0	35.0	2.0	36.0
88-89	29.164292333641022	34.5	30.0	35.0	2.0	36.0
90-91	29.05074100330681	34.0	30.0	35.0	2.0	35.0
92-93	28.95656241713602	34.0	30.0	35.0	2.0	35.0
94-95	28.892598942439207	34.0	30.0	35.0	2.0	35.0
96-97	28.79608900240653	34.0	29.5	35.0	2.0	35.0
98-99	28.735653995558717	34.0	29.0	35.0	2.0	35.0
100-101	28.676469181205924	34.0	29.0	35.0	2.0	35.0
102-103	27.704255438382916	33.0	28.0	34.5	2.0	35.0
104-105	28.52435711220113	34.0	29.0	35.0	2.0	35.0
106-107	28.849471174041923	34.5	30.0	35.0	2.0	35.0
108-109	28.89263653022377	35.0	30.0	35.0	2.0	35.0
110-111	28.838814695411372	35.0	30.0	35.0	2.0	35.0
112-113	28.772488885406005	35.0	30.0	35.0	2.0	35.0
114-115	28.660836991118348	35.0	30.0	35.0	2.0	35.0
116-117	28.604412259176204	35.0	30.0	35.0	2.0	35.0
118-119	28.453266993551303	35.0	30.0	35.0	2.0	35.0
120-121	28.288095105751523	35.0	30.0	35.0	2.0	35.0
122-123	28.170862222773003	35.0	30.0	35.0	2.0	35.0
124-125	27.332043654680795	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82126.0
3	14810.0
4	9695.0
5	8101.0
6	8797.0
7	10152.0
8	11126.0
9	11654.0
10	12568.0
11	13457.0
12	13532.0
13	14351.0
14	16846.0
15	14600.0
16	9701.0
17	8518.0
18	7672.0
19	7217.0
20	6886.0
21	7159.0
22	8323.0
23	11501.0
24	16279.0
25	22337.0
26	27165.0
27	28946.0
28	34053.0
29	40757.0
30	45635.0
31	54694.0
32	69207.0
33	90180.0
34	127210.0
35	198499.0
36	374854.0
37	579103.0
38	177012.0
39	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21735444642401	17.580872972791898	12.483586581948135	24.718185998835963
2	16.47493374835083	19.597272004320317	38.83433555805194	25.093458689276915
3	18.447626677895933	25.084834746562173	29.287561307516956	27.179977268024935
4	13.02624874553474	15.647568411083594	36.379082310239625	34.94710053314204
5	14.03731537494263	37.404066981175504	33.77703660118983	14.781581042692046
6	32.46604958108102	35.40064753418745	17.765209372881873	14.368093511849656
7	29.21110792565728	31.510149721900767	21.377930092101145	17.90081226034081
8	27.60900191288598	33.41438524894637	19.867305784189817	19.109307053977844
9	27.906995821757484	14.603932871028663	19.339866850421835	38.14920445679201
10-11	25.962904699365534	25.02582551047685	27.37410676093975	21.637163029217866
12-13	26.399636292369888	22.992178918630675	27.33577324333317	23.272411545666273
14-15	23.979082121904888	24.058545082639657	24.741921987289572	27.220450808165886
16-17	23.85377320330028	26.997309778800236	25.21214690134602	23.93677011655346
18-19	23.97543659799207	26.09149213117347	26.885588454298492	23.047482816535965
20-21	25.21799386831698	24.986517135660637	26.5161782974892	23.27931069853318
22-23	24.98603380286955	24.647061491610366	26.252894951632854	24.11400975388723
24-25	24.370071820827928	25.29904449371753	26.332008856390026	23.998874829064516
26-27	24.01613817825111	25.288243617023216	26.45530320849082	24.24031499623485
28-29	24.01984223757353	25.24413063148274	26.60840411978275	24.127623011160974
30-31	24.48559764573746	25.142947787591257	26.347542720238053	24.023911846433236
32-33	23.978999331933686	25.247755504595204	26.4168601612522	24.35638500221891
34-35	24.344550339791887	25.270238241742216	26.45107771685377	23.934133701612133
36-37	24.52674814579633	25.03141337032358	26.162385870832416	24.279452613047678
38-39	24.363794763107105	25.398267225004766	26.176896774086195	24.06104123780193
40-41	24.34323073998761	25.18020044607388	26.46684507798544	24.00972373595307
42-43	24.24215209839623	25.234115008342528	26.20962080252373	24.314112090737517
44-45	24.23734531388545	25.23638568442551	26.16200642336299	24.364262578326052
46-47	24.369701925275365	25.32054299517217	26.02275480851535	24.28700027103712
48-49	24.462180687232685	25.503335972573783	25.74693011665078	24.287553223542755
50-51	24.382505224469277	25.63658297715319	25.576002153377065	24.404909645000476
52-53	24.494272916880362	25.57898815832963	25.772033598887635	24.154705325902373
54-55	24.458351737363213	25.437582875112874	25.804295990588077	24.29976939693584
56-57	24.3618044916748	25.54400170291825	25.60407788754267	24.490115917864276
58-59	24.423890770515115	25.34208283830015	25.702905336740695	24.531121054444043
60-61	24.476699735999848	25.563350352247078	25.751739586692207	24.208210325060872
62-63	24.319391194777058	25.58786455305338	25.850269595314174	24.242474656855386
64-65	24.34223600559357	25.246997649898724	25.926115842308022	24.48465050219969
66-67	24.275790842917065	25.610403070439908	25.706889848743188	24.406916237899843
68-69	24.41067378489317	25.50828225311557	25.70801522847151	24.37302873351975
70-71	24.237671150920498	25.32697446767292	25.99383486322171	24.441519518184872
72-73	24.53043704948755	25.52345581559844	25.709972982925645	24.236134151988363
74-75	24.29300762952392	25.73908021825206	25.583153332965658	24.38475881925836
76-77	24.30067973977149	25.429887721820105	25.811151797610783	24.458280740797626
78-79	24.2828895480703	25.481721616554676	25.727604334372238	24.507784501002785
80-81	24.264913639885158	25.67531786902429	25.563824454267873	24.495944036822678
82-83	24.481778956911615	25.622609172347843	25.552341139905156	24.343270730835386
84-85	24.20076675353745	25.61112207312366	25.518319649551803	24.669791523787087
86-87	24.031680694038783	25.776666190467402	25.701545186867197	24.490107928626617
88-89	24.2776868754006	25.506862718067918	25.632731053500198	24.582719353031287
90-91	24.23903845829618	25.48566705201364	25.709926231192693	24.565368258497493
92-93	24.231240182445312	25.798585588570237	25.58025821051754	24.389916018466913
94-95	24.224315861638743	25.383470405173224	25.712561297279763	24.679652435908274
96-97	24.35705502826586	25.464131757441024	25.602949227618506	24.57586398667462
98-99	24.10861886388035	25.515941653893055	25.557497671587164	24.817941810639436
100-101	24.124893574218042	25.585840182137602	25.680754778366673	24.608511465277687
102-103	24.277769538274168	25.333511944508423	25.796032872657747	24.592685644559662
104-105	24.144793167913836	25.54388908203069	25.711725003337584	24.599592746717892
106-107	24.061570609632238	25.547775022087794	25.670277415406822	24.720376952873142
108-109	24.159647017116008	25.574968763584767	25.54594236459891	24.719441854700317
110-111	24.120321272880325	25.728713279560033	25.5497828298304	24.601182617729243
112-113	24.12921942838839	25.52604521577314	25.801350508701937	24.543384847136533
114-115	23.886390913241467	25.865553118333267	25.695504464005005	24.55255150442026
116-117	23.954550290599194	25.88008463156178	25.568878877854935	24.596486199984096
118-119	24.174752404027302	25.386573984211402	25.667658496817847	24.77101511494345
120-121	23.78148563214055	25.607902631329875	25.846852568437484	24.76375916809209
122-123	23.93551217122316	25.691085070384023	25.68028392874437	24.69311882964845
124-125	23.85895944591992	25.703774376666743	25.774533402771038	24.662732774642297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	77.5
2	107.5
3	130.5
4	122.5
5	111.5
6	141.0
7	186.0
8	252.0
9	324.5
10	388.5
11	449.0
12	521.5
13	643.5
14	782.5
15	890.0
16	1026.5
17	1156.5
18	1266.0
19	1497.5
20	1690.5
21	1873.0
22	2167.0
23	2527.5
24	2938.5
25	3927.0
26	5805.0
27	7743.5
28	8833.0
29	10681.0
30	14143.5
31	17455.0
32	21935.5
33	26944.5
34	31907.5
35	36615.5
36	41621.5
37	46602.5
38	48605.5
39	50640.0
40	52720.5
41	54261.5
42	56245.5
43	61718.0
44	67828.5
45	71501.5
46	74415.5
47	76459.5
48	81229.5
49	86361.5
50	86002.0
51	84109.0
52	83379.5
53	82794.0
54	83552.0
55	83036.5
56	79144.0
57	73076.0
58	69086.0
59	64716.0
60	57941.0
61	51631.5
62	44572.0
63	35200.0
64	27167.0
65	21933.5
66	17123.0
67	13508.5
68	11092.0
69	9143.5
70	7991.5
71	6666.5
72	5412.0
73	4951.5
74	4186.5
75	3261.5
76	2311.5
77	1510.5
78	1012.5
79	657.0
80	404.5
81	219.5
82	141.5
83	84.0
84	54.5
85	41.5
86	31.5
87	19.5
88	15.5
89	13.0
90	10.0
91	11.5
92	9.0
93	12.0
94	14.5
95	11.5
96	8.0
97	10.5
98	14.5
99	11.5
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03521861511110949
2	0.02628866871812442
3	0.027154326786832154
4	0.0318926656892324
5	0.034307396091417135
6	0.04560651193560233
7	0.03922797879775585
8	0.03622095603277108
9	0.029067886728186103
10-11	0.03692715077303266
12-13	0.03608427317981723
14-15	0.03453520084634022
16-17	0.036653785067124946
18-19	0.050595436068417966
20-21	0.06091499146643387
22-23	0.053420215029464264
24-25	0.0645826480206956
26-27	0.04820348614172554
28-29	0.04815792519074092
30-31	0.04802124233778707
32-33	0.055470457823772065
34-35	0.039729149258586643
36-37	0.05150665508811032
38-39	0.060527723383064634
40-41	0.048066803288771685
42-43	0.05840913916227991
44-45	0.05954816293689535
46-47	0.06540274513841872
48-49	0.04779343758286398
50-51	0.050048704656602556
52-53	0.06091499146643387
54-55	0.04779343758286398
56-57	0.04469529291590997
58-59	0.04490031719534075
60-61	0.03346451849820171
62-63	0.042166660136263696
64-65	0.04164270919994059
66-67	0.035491980817017196
68-69	0.031095349047001588
70-71	0.04895524183297173
72-73	0.031619299983324696
74-75	0.04032144162138668
76-77	0.032507738527524734
78-79	0.03310003089032477
80-81	0.025605254453355153
82-83	0.018566087526231716
84-85	0.01890779465861635
86-87	0.01833828277130863
88-89	0.023031060722724254
90-91	0.013804968148339166
92-93	0.020547988894062588
94-95	0.015103455251400772
96-97	0.01154970107460059
98-99	0.010661262530400544
100-101	0.01173194487853906
102-103	0.006856923123184966
104-105	0.007244191206554216
106-107	0.009613360657754337
108-109	0.014032772903262256
110-111	0.0070391669271234365
112-113	0.009453897329308176
114-115	0.008929946392985071
116-117	0.007859264044846555
118-119	0.2417919668753662
120-121	0.35505649102312586
122-123	0.029591837664509207
124-125	0.004054924637630976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2194862.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.78459786973587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06391597733466	41.73357010870195
2	10.677238529951092	11.271874843254363
3	3.688366424839065	5.840668155940962
4	1.806396549569838	3.8139966184928915
5	1.040006300653064	2.74481571809818
6	0.7030094972332017	2.2264844166035846
7	0.49656517613255197	1.8347695196789922
8	0.34972194753916713	1.4767945893660641
9	0.280720644612612	1.3335953705648782
>10	1.7037829896886518	16.669314929065003
>50	0.12781795479675376	4.5956529129692365
>100	0.06019148958883565	5.545722671217016
>500	0.001917822974303277	0.6049963420158292
>1k	3.4869508623694325E-4	0.30774380403116103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2325	0.10592921103923618	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.556095098461771E-5
2	0.0	0.0	0.0	0.0	4.556095098461771E-5
3	0.0	0.0	0.0	0.0	4.556095098461771E-5
4	0.0	0.0	0.0	0.0	4.556095098461771E-5
5	0.0	0.0	0.0	0.0	4.556095098461771E-5
6	0.0	0.0	0.0	0.0	4.556095098461771E-5
7	0.0	0.0	0.0	0.0	4.556095098461771E-5
8	0.0	0.0	0.0	0.0	9.112190196923542E-5
9	0.0	0.0	0.0	0.0	1.3668285295385314E-4
10-11	0.0	0.0	0.0	0.0	1.3668285295385314E-4
12-13	0.0	0.0	0.0	2.2780475492308856E-5	2.2780475492308855E-4
14-15	0.0	0.0	0.0	4.556095098461771E-5	2.9614618140001513E-4
16-17	0.0	0.0	0.0	4.556095098461771E-5	3.644876078769417E-4
18-19	0.0	0.0	0.0	4.556095098461771E-5	4.100485588615594E-4
20-21	0.0	0.0	0.0	4.556095098461771E-5	4.100485588615594E-4
22-23	0.0	0.0	0.0	4.556095098461771E-5	4.556095098461771E-4
24-25	0.0	0.0	0.0	1.3668285295385314E-4	5.011704608307949E-4
26-27	0.0	0.0	0.0	2.050242794307797E-4	5.695118873077214E-4
28-29	0.0	0.0	0.0	8.428775932154276E-4	5.922923628000303E-4
30-31	0.0	0.0	0.0	0.004168827015092521	5.922923628000303E-4
32-33	0.0	0.0	0.0	0.010638482054908235	6.37853313784648E-4
34-35	0.0	0.0	0.0	0.018725550854677878	6.834142647692657E-4
36-37	0.0	0.0	0.0	0.029341252434093804	6.834142647692657E-4
38-39	0.0	0.0	0.0	0.048932461357479425	6.834142647692657E-4
40-41	0.0	0.0	0.0	0.07007274261434204	6.834142647692657E-4
42-43	0.0	0.0	0.0	0.09066629245938924	7.289752157538834E-4
44-45	0.0	0.0	0.0	0.11629432738823671	8.200971177231188E-4
46-47	0.0	0.0	0.0	0.14451933652320736	8.884385442000453E-4
48-49	0.0	0.0	0.0	0.17663980696736287	9.567799706769719E-4
50-51	0.0	0.0	0.0	0.21181286112748776	9.567799706769719E-4
52-53	0.0	0.0	0.0	0.2451406967727356	0.0010479018726462074
54-55	0.0	0.0	0.0	0.2818628232663375	0.0011390237746154428
56-57	0.0	0.0	0.0	0.32375611769669344	0.0011390237746154428
58-59	0.0	0.0	0.0	0.3714812138530805	0.0011390237746154428
60-61	0.0	0.0	0.0	0.4216438208871446	0.0011390237746154428
62-63	0.0	0.0	0.0	0.47547408447547046	0.0011390237746154428
64-65	2.2780475492308856E-5	0.0	0.0	0.5310812251521964	0.0011390237746154428
66-67	4.556095098461771E-5	0.0	0.0	0.5953221660405074	0.0012073652010923695
68-69	4.556095098461771E-5	0.0	0.0	0.667354029547188	0.001275706627569296
70-71	4.556095098461771E-5	0.0	0.0	0.7509128136529768	0.001412389480523149
72-73	4.556095098461771E-5	0.0	0.0	0.8339932077734271	0.0016401942354462376
74-75	4.556095098461771E-5	0.0	0.0	0.9193060884921238	0.0016629747109385465
76-77	4.556095098461771E-5	0.0	0.0	1.015781402202052	0.0016857551864308553
78-79	4.556095098461771E-5	0.0	0.0	1.1282941706585654	0.0016857551864308553
80-81	4.556095098461771E-5	0.0	0.0	1.250033031689464	0.0016857551864308553
82-83	4.556095098461771E-5	0.0	0.0	1.3808385219663013	0.0016857551864308553
84-85	4.556095098461771E-5	0.0	0.0	1.5130108407726772	0.0016857551864308553
86-87	4.556095098461771E-5	0.0	0.0	1.6571884701635	0.001731316137415473
88-89	4.556095098461771E-5	0.0	0.0	1.818428675698062	0.0017768770884000907
90-91	4.556095098461771E-5	0.0	0.0	1.9999662848962712	0.0017768770884000907
92-93	4.556095098461771E-5	0.0	0.0	2.192096815198404	0.0019135599413539438
94-95	4.556095098461771E-5	2.2780475492308856E-5	0.0	2.3998547516882613	0.0019591208923385615
96-97	4.556095098461771E-5	4.556095098461771E-5	0.0	2.616633756473072	0.002027462318815488
98-99	4.556095098461771E-5	4.556095098461771E-5	0.0	2.848812362690684	0.002095803745292415
100-101	4.556095098461771E-5	4.556095098461771E-5	0.0	3.11507056024479	0.002095803745292415
102-103	4.556095098461771E-5	4.556095098461771E-5	0.0	3.4089614745710666	0.002164145171769341
104-105	4.556095098461771E-5	4.556095098461771E-5	0.0	3.7109622381726046	0.0022780475492308857
106-107	4.556095098461771E-5	4.556095098461771E-5	0.0	4.023464800976098	0.0023008280247231944
108-109	4.556095098461771E-5	4.556095098461771E-5	0.0	4.349954575731869	0.00239194992669243
110-111	4.556095098461771E-5	4.556095098461771E-5	0.0	4.706218431956087	0.0026425351571078273
112-113	4.556095098461771E-5	4.556095098461771E-5	4.556095098461771E-5	5.0941471491146135	0.0027336570590770627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2655	0.0	72.61238	1
GTATCAA	5540	0.0	60.790665	1
TCAACGC	6985	0.0	47.528965	4
CAACGCA	7040	0.0	47.24216	5
AACGCAG	7405	0.0	44.668438	6
ATCAACG	7460	0.0	44.49759	3
TATCAAC	8000	0.0	41.791447	2
ACGCAGA	8550	0.0	38.616943	7
GTACATG	11645	0.0	37.50479	1
CGCAGAG	8960	0.0	36.451496	8
TACATGG	12230	0.0	35.314354	2
ACATGGG	12190	0.0	35.039818	3
GCAGAGT	9660	0.0	33.3174	9
CATGGGG	9490	0.0	32.538067	4
ATGGGGG	5150	0.0	28.419634	5
AGAGTAC	8825	0.0	27.80739	10-11
CAGAGTA	9435	0.0	26.923838	10-11
GAGTACT	3785	0.0	22.477646	12-13
GTATAGG	750	0.0	22.214008	1
GTGGTAT	1275	0.0	21.46732	1
>>END_MODULE