FastQCFastQC Report
Mon 15 Aug 2016
SRR2921548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194862
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.15012333349431536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.13363026923788376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.077.367481
GTATCAA69300.060.4682731
TCAACGC92500.044.4831854
CAACGCA94900.043.2658655
ATCAACG98200.042.2043573
AACGCAG99400.041.2388276
TATCAAC103000.040.411912
GTACATG118500.038.4811941
TACATGG123350.036.400022
ACATGGG122100.036.332913
ACGCAGA114450.035.6600387
CGCAGAG121050.033.6658338
CATGGGG97050.032.51924
GCAGAGT127200.031.3365579
ATGGGGG51200.028.8233365
AGAGTAC121100.028.14972110-11
CAGAGTA126450.026.06480810-11
GTGGTAT13400.025.800531
GAGTACT69200.025.10385112-13
AGTACTT76050.023.62497112-13
Produced by FastQC (version 0.11.5)