##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2194862
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248349554550582	31.0	31.0	34.0	28.0	34.0
2	31.31081771883608	33.0	31.0	34.0	28.0	34.0
3	31.385157700119642	33.0	31.0	34.0	28.0	34.0
4	34.8825042303343	37.0	35.0	37.0	32.0	37.0
5	34.53816230815423	37.0	35.0	37.0	31.0	37.0
6	34.51257300003372	37.0	35.0	37.0	31.0	37.0
7	34.352207109148544	37.0	35.0	37.0	30.0	37.0
8	34.3222662745995	37.0	35.0	37.0	30.0	37.0
9	35.88766993095693	39.0	37.0	39.0	30.0	39.0
10-11	35.7554766541131	39.0	37.0	39.0	30.0	39.0
12-13	35.664589163236684	39.0	36.0	39.0	30.0	39.0
14-15	36.83708201244543	40.0	37.0	41.0	30.0	41.0
16-17	36.82422539549184	40.0	37.0	41.0	30.0	41.0
18-19	36.85662561017504	40.0	37.0	41.0	30.0	41.0
20-21	36.84283271567871	40.0	37.0	41.0	30.0	41.0
22-23	36.781645041920626	40.0	37.0	41.0	30.0	41.0
24-25	36.71605618029744	40.0	37.0	41.0	30.0	41.0
26-27	36.59364780109182	40.0	36.0	41.0	29.5	41.0
28-29	36.4118575564204	40.0	36.0	41.0	29.0	41.0
30-31	36.360304429162284	40.0	36.0	41.0	29.0	41.0
32-33	36.22986821039318	40.0	35.5	41.0	29.0	41.0
34-35	36.098098194784	40.0	35.0	41.0	28.5	41.0
36-37	35.9575515453819	40.0	35.0	41.0	28.0	41.0
38-39	35.779837866799824	40.0	35.0	41.0	27.0	41.0
40-41	35.57798121248625	40.0	35.0	41.0	26.5	41.0
42-43	35.40361079648743	39.0	35.0	41.0	26.0	41.0
44-45	35.179348405503404	39.0	35.0	41.0	25.0	41.0
46-47	34.88462850967396	39.0	34.5	41.0	23.5	41.0
48-49	34.61509083486798	39.0	34.0	41.0	23.0	41.0
50-51	34.39831843642106	39.0	34.0	41.0	22.0	41.0
52-53	34.209157113294594	38.5	34.0	41.0	21.5	41.0
54-55	33.991024264851276	38.0	33.5	40.0	20.5	41.0
56-57	33.739920778618426	38.0	33.0	40.0	20.0	41.0
58-59	33.5996992066016	37.0	33.0	40.0	20.0	41.0
60-61	33.492357833886594	37.0	33.0	40.0	20.0	41.0
62-63	33.23097329125932	36.5	33.0	40.0	19.0	41.0
64-65	32.938802302832705	36.0	33.0	39.5	18.0	41.0
66-67	32.64461159744895	35.5	33.0	39.0	18.0	41.0
68-69	32.32303352101408	35.0	32.5	39.0	16.0	41.0
70-71	32.025342139961424	35.0	32.0	37.5	14.0	40.0
72-73	31.714199343740063	35.0	32.0	37.0	13.0	39.5
74-75	31.42287533339226	35.0	32.0	37.0	11.5	39.0
76-77	31.133858757407072	35.0	32.0	36.0	9.0	39.0
78-79	30.855270627492754	35.0	31.5	36.0	7.5	37.5
80-81	30.626197683499008	35.0	31.0	35.0	7.0	37.0
82-83	30.43951464830135	35.0	31.0	35.0	7.0	37.0
84-85	30.241404698791996	35.0	31.0	35.0	5.5	36.0
86-87	30.096481692243067	35.0	31.0	35.0	2.0	36.0
88-89	29.98422976023094	35.0	31.0	35.0	2.0	36.0
90-91	29.881807147784237	35.0	31.0	35.0	2.0	35.0
92-93	29.943080931739672	35.0	31.0	35.0	2.0	35.0
94-95	29.905882465503524	35.0	31.0	35.0	2.0	35.0
96-97	29.88324596261633	35.0	31.0	35.0	2.0	35.0
98-99	29.842457521247347	35.0	31.0	35.0	2.0	35.0
100-101	29.798610117629266	35.0	31.0	35.0	2.0	35.0
102-103	28.92284116267902	33.5	29.5	35.0	2.0	35.0
104-105	29.61891818255544	34.5	31.0	35.0	2.0	35.0
106-107	29.728884321656672	35.0	31.0	35.0	2.0	35.0
108-109	29.73379396973477	35.0	31.0	35.0	2.0	35.0
110-111	29.698794958407404	35.0	31.0	35.0	2.0	35.0
112-113	29.653553389689193	35.0	31.0	35.0	2.0	35.0
114-115	29.593456445097686	35.0	31.0	35.0	2.0	35.0
116-117	29.518132119468106	35.0	31.0	35.0	2.0	35.0
118-119	29.4299432037185	35.0	31.0	35.0	2.0	35.0
120-121	29.309389155217957	35.0	31.0	35.0	2.0	35.0
122-123	29.194183734558255	35.0	31.0	35.0	2.0	35.0
124-125	28.290764066260202	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37573.0
3	13902.0
4	7722.0
5	6492.0
6	6770.0
7	8135.0
8	9285.0
9	10304.0
10	12212.0
11	13350.0
12	13898.0
13	16182.0
14	20830.0
15	17393.0
16	9072.0
17	7653.0
18	6811.0
19	6272.0
20	6070.0
21	6265.0
22	7397.0
23	10024.0
24	14344.0
25	20085.0
26	25209.0
27	27477.0
28	32301.0
29	38479.0
30	44720.0
31	53400.0
32	67006.0
33	86049.0
34	119661.0
35	191397.0
36	378786.0
37	629539.0
38	212423.0
39	374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.279806856723184	18.162310603036737	12.815152213233096	24.74273032700699
2	16.634986185730842	20.046864634741976	38.02450483681667	25.293644342710515
3	18.39703356560438	25.212915126965267	29.431708347403116	26.95834296002723
4	13.066717358620961	16.13750101429495	36.025933128559714	34.76984849852437
5	14.041463905930563	37.52828928119933	33.53333691838924	14.896909894480867
6	32.59535549256751	35.88793394154264	17.139289652846763	14.377420913043082
7	29.0322845233967	31.918839618157786	21.02456164653849	18.02431421190703
8	27.73404715799066	33.476204579109236	19.415468732201845	19.374279530698256
9	27.894012639022414	14.306653483932402	19.239653907663147	38.55967996938204
10-11	26.070487018283124	25.095115418858686	27.099168816540924	21.73522874631727
12-13	26.567749019822255	22.98837455661338	27.407842788830646	23.03603363473372
14-15	24.1540522001648	23.86110999104019	25.01321873527059	26.971619073524415
16-17	23.73733242900453	26.799512172783608	25.481544834788743	23.98161056342312
18-19	23.771238558727102	25.59063360793679	27.727195994241686	22.910931839094417
20-21	24.91636772909592	24.5325580891994	27.488047243717755	23.063026937986926
22-23	24.671662591235847	24.231251784163845	27.232246701133732	23.864838923466575
24-25	24.13564559954469	24.86039323653931	27.268624146012154	23.73533701790384
26-27	23.741385320243957	24.843380850293098	27.49707812845299	23.918155701009955
28-29	23.84874769029571	24.855949638763388	27.491780756375466	23.803521914565433
30-31	24.218619162728043	24.740037199225938	27.360547903630174	23.680795734415845
32-33	23.7933507672805	24.84116471161308	27.290552267894057	24.07493225321236
34-35	24.040992451708984	24.896122136124433	27.365471444638274	23.697413967528313
36-37	24.2925878130713	24.69009563700589	27.021350182361015	23.995966367561795
38-39	24.253008336667577	25.031694283909694	26.92945504059876	23.78584233882397
40-41	24.228098745765834	24.842298028930408	27.147271131654033	23.782332093649728
42-43	24.17570775914489	24.934460100913334	26.80044772402776	24.089384415914026
44-45	24.173521707985266	24.924492775548966	26.709416119994966	24.1925693964708
46-47	24.36248514764635	25.092235536968833	26.357980740641167	24.18729857474365
48-49	24.523044476657947	25.373851558544597	25.8360763165294	24.26702764826806
50-51	24.38506969870819	25.568610313795702	25.64486855194104	24.401451435555067
52-53	24.533670616344295	25.450869566307123	25.82142669164238	24.194033125706202
54-55	24.53676225152058	25.39712846709175	25.81727499433235	24.248834287055317
56-57	24.38061798072826	25.47569842394635	25.65071169633682	24.492971898988564
58-59	24.36680950092766	25.26291278452077	25.895334316244483	24.474943398307087
60-61	24.484775324446293	25.46685222729719	25.921639823717403	24.12673262453912
62-63	24.33180966729304	25.48660133153875	25.950764647028763	24.23082435413945
64-65	24.348309512254584	25.122135717491805	26.049597058079687	24.479957712173928
66-67	24.332414185601923	25.46446274740494	25.817669736086962	24.38545333090617
68-69	24.464847552416956	25.382919053122414	25.78103638106974	24.371197013390887
70-71	24.280080481797157	25.179770552602637	26.11712238614722	24.42302657945299
72-73	24.600991446455993	25.410343861993134	25.70770488826348	24.2809598032874
74-75	24.37958625868834	25.627932665075694	25.566664828707523	24.425816247528438
76-77	24.29303590326612	25.399854869559174	25.790545104923385	24.51656412225132
78-79	24.334452339978707	25.435305130598373	25.65315138765331	24.577091141769607
80-81	24.32924595196127	25.600894277969882	25.517545602525704	24.55231416754314
82-83	24.4933197882946	25.50744343013601	25.508582562121994	24.490654219447396
84-85	24.1672909307641	25.563364172891077	25.456646908935816	24.812697987409003
86-87	24.04459008684395	25.64440344423061	25.63761395463391	24.673392514291535
88-89	24.26103359939701	25.38127173288182	25.56808821730045	24.789606450420717
90-91	24.165881587686012	25.378757414582076	25.743064439510565	24.712296558221343
92-93	24.18249713569569	25.613407655261927	25.594019920384987	24.610075288657402
94-95	24.11954106575702	25.278700647721426	25.71270202422343	24.88905626229813
96-97	24.25223671180299	25.381271284727898	25.562882737822274	24.803609265646845
98-99	24.080564090788144	25.3592315374718	25.48377455322158	25.076429818518477
100-101	24.03264460635162	25.46016833557026	25.63844647179025	24.86874058628787
102-103	24.228094195585463	25.169343833934377	25.747097109297222	24.855464861182934
104-105	24.099198586483244	25.37263484598549	25.629399726946687	24.89876684058457
106-107	24.032111460665966	25.39580063375485	25.551323435944518	25.02076446963467
108-109	24.112670600833923	25.384264849962406	25.419945772289182	25.083118776914485
110-111	24.10954865999466	25.49028586112349	25.441535199344646	24.958630279537207
112-113	24.005126102111955	25.366322648258265	25.68138427539699	24.947166974232793
114-115	23.810504474860547	25.639814162183	25.53729992067676	25.012381442279697
116-117	23.86379998213866	25.61539611169535	25.432157886966873	25.088646019199118
118-119	23.879542886304815	25.179961178143113	25.611170852622756	25.329325082929316
120-121	23.554441799273274	25.445956874850495	25.710881525441103	25.28871980043512
122-123	23.725182165649205	25.44510017345204	25.560051447871214	25.269666213027538
124-125	23.672633923770046	25.515675101216416	25.53410915878719	25.277581816226345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	21.5
2	34.0
3	46.0
4	44.0
5	52.0
6	88.5
7	195.0
8	323.5
9	390.5
10	404.0
11	410.0
12	485.5
13	610.0
14	731.0
15	916.0
16	1115.0
17	1330.0
18	1620.5
19	1856.0
20	2108.0
21	2415.5
22	2777.5
23	3263.0
24	3777.0
25	4772.5
26	6624.5
27	8538.5
28	9505.5
29	11204.0
30	14723.0
31	17938.5
32	21985.5
33	26627.5
34	31397.5
35	36008.5
36	41046.0
37	45990.0
38	47787.5
39	49511.5
40	51830.5
41	53334.5
42	55350.5
43	60998.0
44	67075.5
45	70955.0
46	74179.5
47	76769.5
48	81792.0
49	86426.5
50	85617.0
51	84112.0
52	83445.5
53	83346.5
54	84749.0
55	83851.5
56	79760.5
57	73633.0
58	69113.0
59	64919.5
60	58240.0
61	51916.5
62	44803.5
63	34849.0
64	26578.0
65	21584.5
66	16909.0
67	13032.0
68	10758.0
69	8854.5
70	7678.0
71	6467.0
72	5268.0
73	4976.0
74	4087.0
75	3122.0
76	2233.5
77	1402.5
78	912.0
79	539.5
80	326.5
81	166.5
82	91.0
83	49.5
84	28.5
85	19.0
86	9.0
87	3.5
88	3.0
89	3.5
90	3.0
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12333349431536014
2	0.0013668285295385314
3	0.020183501286185648
4	0.05558436020123361
5	0.02587862015926286
6	0.020137940335201027
7	0.015627406187723875
8	0.005102826510277184
9	0.0032803884708924747
10-11	0.007289752157538834
12-13	0.004875021755354095
14-15	0.002756437534569372
16-17	0.010592921103923618
18-19	0.005535655544631052
20-21	0.01296209055512374
22-23	0.009408336378323558
24-25	0.013896090050308403
26-27	0.009431116853815867
28-29	0.014442821462123815
30-31	0.021026378879401075
32-33	0.013349358638492988
34-35	0.020866915550954913
36-37	0.014306138609169962
38-39	0.02095803745292415
40-41	0.009841165412677426
42-43	0.009408336378323558
44-45	0.016857551864308554
46-47	0.0156957476142008
48-49	0.02164145171769341
50-51	0.01571852808969311
52-53	0.02111750078137031
54-55	0.017745990408508598
56-57	0.020821354599970296
58-59	0.016424722829954684
60-61	0.019636769874370234
62-63	0.022028719801062663
64-65	0.01717647852120088
66-67	0.011686383927554443
68-69	0.02432954782578586
70-71	0.01496677239844692
72-73	0.017837112310477833
74-75	0.018178819442862468
76-77	0.01296209055512374
78-79	0.029796861943939986
80-81	0.021231403158831853
82-83	0.009499458280292793
84-85	0.012665944373723724
86-87	0.013372139113985299
88-89	0.021071939830385692
90-91	0.01296209055512374
92-93	0.007995946897800407
94-95	0.007677020240908084
96-97	0.0030298032404770777
98-99	0.001731316137415473
100-101	0.01173194487853906
102-103	0.0030525837159693864
104-105	0.00239194992669243
106-107	3.18926656892324E-4
108-109	0.018771111805662495
110-111	9.112190196923542E-4
112-113	0.009658921608738956
114-115	0.0020502427943077974
116-117	0.008109849275261953
118-119	0.010114531118585133
120-121	0.0039638027356617405
122-123	8.656580687077365E-4
124-125	0.02524076684547821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2194862.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.55031166140053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45612851541132	40.444378767163464
2	11.037302980005025	11.379528170411275
3	3.6707434209782	5.676839021413853
4	1.8542805011599508	3.823549509698137
5	1.112305049601037	2.866983598474151
6	0.7435421721315639	2.29978984240661
7	0.49063002307465126	1.770449141995688
8	0.3733840779731032	1.5398452471134516
9	0.27952164707905197	1.2968485220729822
>10	1.7809759740356454	17.092913448194075
>50	0.13265380203878607	4.711793467742184
>100	0.0655965902054423	5.883689668107358
>500	0.0025794588751782816	0.7870102913550139
>1k	3.557874310590628E-4	0.42638130385167117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3295	0.15012333349431536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2933	0.13363026923788376	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.556095098461771E-5	0.0
2	0.0	0.0	0.0	4.556095098461771E-5	0.0
3	0.0	0.0	0.0	4.556095098461771E-5	0.0
4	0.0	0.0	0.0	4.556095098461771E-5	0.0
5	0.0	0.0	0.0	4.556095098461771E-5	0.0
6	0.0	0.0	0.0	4.556095098461771E-5	0.0
7	0.0	0.0	0.0	4.556095098461771E-5	4.556095098461771E-5
8	0.0	0.0	0.0	4.556095098461771E-5	9.112190196923542E-5
9	0.0	0.0	0.0	4.556095098461771E-5	9.112190196923542E-5
10-11	0.0	0.0	0.0	4.556095098461771E-5	9.112190196923542E-5
12-13	0.0	0.0	0.0	6.834142647692657E-5	9.112190196923542E-5
14-15	0.0	0.0	0.0	9.112190196923542E-5	1.59463328446162E-4
16-17	0.0	0.0	0.0	9.112190196923542E-5	1.8224380393847084E-4
18-19	0.0	0.0	0.0	9.112190196923542E-5	2.2780475492308855E-4
20-21	0.0	0.0	0.0	9.112190196923542E-5	2.2780475492308855E-4
22-23	0.0	0.0	0.0	1.1390237746154429E-4	2.9614618140001513E-4
24-25	0.0	0.0	0.0	2.733657059077063E-4	3.18926656892324E-4
26-27	0.0	0.0	0.0	3.644876078769417E-4	3.18926656892324E-4
28-29	0.0	0.0	0.0	0.0010479018726462074	3.18926656892324E-4
30-31	0.0	0.0	0.0	0.004419412245507918	3.4170713238463284E-4
32-33	0.0	0.0	0.0	0.010911847760815942	3.644876078769417E-4
34-35	0.0	0.0	0.0	0.01918116036452406	3.644876078769417E-4
36-37	0.0	0.0	0.0	0.030138569076324617	3.8726808336925054E-4
38-39	0.0	0.0	0.0	0.04952475372027945	4.556095098461771E-4
40-41	0.0	0.0	0.0	0.0710067421095267	4.556095098461771E-4
42-43	0.0	0.0	0.0	0.09214702336638932	4.78389985338486E-4
44-45	0.0	0.0	0.0	0.11800286305015988	5.011704608307949E-4
46-47	0.0	0.0	0.0	0.14654679884202287	6.150728382923391E-4
48-49	0.0	0.0	0.0	0.17962404925685532	7.289752157538834E-4
50-51	0.0	0.0	0.0	0.215298273877811	7.289752157538834E-4
52-53	0.0	0.0	0.0	0.2493095237878281	8.428775932154276E-4
54-55	0.0	0.0	0.0	0.2862822355118454	9.567799706769719E-4
56-57	0.0	0.0	0.0	0.3284261151726168	9.795604461692808E-4
58-59	0.0	0.0	0.0	0.3770624303486962	0.0010251213971538987
60-61	0.0	0.0	0.0	0.428751329240745	0.0010479018726462074
62-63	0.0	0.0	0.0	0.4830144218634247	0.0010934628236308251
64-65	0.0	0.0	0.0	0.5398517082167353	0.0010934628236308251
66-67	0.0	0.0	0.0	0.6051633314531848	0.001116243299123134
68-69	0.0	0.0	0.0	0.6782886577834962	0.0011390237746154428
70-71	0.0	0.0	0.0	0.7633509532717775	0.0012301456765846782
72-73	0.0	0.0	0.0	0.847912078299228	0.001412389480523149
74-75	0.0	0.0	0.0	0.9346373484984478	0.0014579504315077668
76-77	0.0	0.0	0.0	1.0332540269046526	0.0014807309070000757
78-79	0.0	0.0	0.0	1.1473386481701355	0.0015490723334770022
80-81	0.0	0.0	0.0	1.2706038010590186	0.0015946332844616199
82-83	0.0	0.0	0.0	1.4034595341301639	0.001731316137415473
84-85	0.0	0.0	0.0	1.5380238028632323	0.0017996575638923994
86-87	0.0	0.0	0.0	1.6850034307396091	0.0018224380393847084
88-89	0.0	0.0	0.0	1.84909119571071	0.0018452185148770174
90-91	0.0	0.0	0.0	2.0329296329336426	0.001867998990369326
92-93	0.0	0.0	0.0	2.2287050393145442	0.0019135599413539438
94-95	0.0	0.0	0.0	2.4396522423733247	0.0021413646962770323
96-97	0.0	0.0	0.0	2.6621719269821975	0.002209706122753959
98-99	0.0	0.0	0.0	2.8999317496954253	0.0022780475492308857
100-101	0.0	0.0	0.0	3.1705182375930696	0.0022780475492308857
102-103	0.0	0.0	0.0	3.469329734625685	0.002369169451200121
104-105	6.834142647692657E-5	0.0	0.0	3.7781418603994235	0.002369169451200121
106-107	9.112190196923542E-5	0.0	0.0	4.0976380291790555	0.002369169451200121
108-109	9.112190196923542E-5	0.0	0.0	4.43048355659718	0.00239194992669243
110-111	9.112190196923542E-5	0.0	0.0	4.795586237312414	0.0025514132551385914
112-113	9.112190196923542E-5	0.0	0.0	5.193242217506158	0.0025969742061232094
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3390	0.0	77.36748	1
GTATCAA	6930	0.0	60.468273	1
TCAACGC	9250	0.0	44.483185	4
CAACGCA	9490	0.0	43.265865	5
ATCAACG	9820	0.0	42.204357	3
AACGCAG	9940	0.0	41.238827	6
TATCAAC	10300	0.0	40.41191	2
GTACATG	11850	0.0	38.481194	1
TACATGG	12335	0.0	36.40002	2
ACATGGG	12210	0.0	36.33291	3
ACGCAGA	11445	0.0	35.660038	7
CGCAGAG	12105	0.0	33.665833	8
CATGGGG	9705	0.0	32.5192	4
GCAGAGT	12720	0.0	31.336557	9
ATGGGGG	5120	0.0	28.823336	5
AGAGTAC	12110	0.0	28.149721	10-11
CAGAGTA	12645	0.0	26.064808	10-11
GTGGTAT	1340	0.0	25.80053	1
GAGTACT	6920	0.0	25.103851	12-13
AGTACTT	7605	0.0	23.624971	12-13
>>END_MODULE