FastQCFastQC Report
Mon 15 Aug 2016
SRR2921547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919793
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.1058968336690466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.070.454231
GTATCAA45250.060.8934331
TCAACGC59300.045.449284
CAACGCA59800.044.9697765
AACGCAG63500.042.4420786
ATCAACG64500.042.0585863
TATCAAC67300.041.1032452
ACGCAGA73800.036.357367
GTACATG97900.035.3792231
CGCAGAG77850.034.3868268
TACATGG100850.033.6230552
ACATGGG102850.032.796563
GCAGAGT84150.030.540719
CATGGGG86450.030.2122444
AGAGTAC75700.027.78105410-11
ATGGGGG46800.027.4594215
CAGAGTA79550.025.80085610-11
GAGTACT32550.023.02928412-13
GTGGTAT12050.021.7306981
GTACTTT34900.021.65329714-15
Produced by FastQC (version 0.11.5)