##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921547_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1919793
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.776628001039697	31.0	30.0	34.0	25.0	34.0
2	29.812297471654496	31.0	30.0	34.0	25.0	34.0
3	29.881109057070216	31.0	30.0	34.0	25.0	34.0
4	33.244696172972816	37.0	35.0	37.0	26.0	37.0
5	33.23511128543546	37.0	35.0	37.0	26.0	37.0
6	33.25165369391387	37.0	35.0	37.0	26.0	37.0
7	33.05451473153616	37.0	35.0	37.0	25.0	37.0
8	33.01942761537312	37.0	35.0	37.0	25.0	37.0
9	34.45515584232258	39.0	35.0	39.0	25.0	39.0
10-11	34.34620295000555	39.0	35.0	39.0	25.0	39.0
12-13	34.2301826290647	39.0	35.0	39.0	24.0	39.0
14-15	35.37628640171101	40.0	36.0	41.0	24.0	41.0
16-17	35.326478688066885	40.0	36.0	41.0	24.0	41.0
18-19	35.35738592650354	40.0	36.0	41.0	24.0	41.0
20-21	35.38111140107293	40.0	36.0	41.0	24.0	41.0
22-23	35.35431580383927	40.0	35.0	41.0	24.0	41.0
24-25	35.32685424939043	40.0	35.0	41.0	24.0	41.0
26-27	35.25189668886177	40.0	35.0	41.0	24.0	41.0
28-29	35.21444551574049	40.0	35.0	41.0	24.0	41.0
30-31	35.11405083777261	40.0	35.0	41.0	24.0	41.0
32-33	35.00336051855591	40.0	35.0	41.0	24.0	41.0
34-35	34.83943581417371	40.0	34.5	41.0	23.0	41.0
36-37	34.70522837618431	39.0	34.0	41.0	22.0	41.0
38-39	34.57996721521539	39.0	34.0	41.0	21.5	41.0
40-41	34.4283209179323	39.0	34.0	41.0	19.5	41.0
42-43	34.238089210659695	39.0	33.5	41.0	18.0	41.0
44-45	34.04241629175645	39.0	33.0	41.0	16.5	41.0
46-47	33.776012570105216	39.0	33.0	41.0	14.5	41.0
48-49	33.612597556090684	38.0	33.0	41.0	11.5	41.0
50-51	33.422278860272954	38.0	33.0	40.0	9.0	41.0
52-53	33.23510920187749	38.0	33.0	40.0	8.0	41.0
54-55	33.03301215287273	38.0	32.5	40.0	7.5	41.0
56-57	32.80126190688267	37.0	32.0	40.0	6.5	41.0
58-59	32.56442257055839	37.0	32.0	40.0	2.0	41.0
60-61	32.26268717512774	36.0	31.5	40.0	2.0	41.0
62-63	31.9511952590722	36.0	31.0	39.5	2.0	41.0
64-65	31.64438796266056	35.0	31.0	39.0	2.0	41.0
66-67	31.598802058346916	35.0	31.0	39.0	2.0	41.0
68-69	31.354510095619684	35.0	31.0	38.5	2.0	40.5
70-71	31.05295050039249	35.0	31.0	37.5	2.0	40.0
72-73	30.723728287372648	35.0	31.0	37.0	2.0	39.0
74-75	30.418729519276297	35.0	31.0	36.5	2.0	39.0
76-77	30.13808103269467	35.0	30.0	36.0	2.0	39.0
78-79	29.8599489632476	35.0	30.0	35.5	2.0	37.0
80-81	29.619252961126538	35.0	30.0	35.0	2.0	37.0
82-83	29.41712179386007	35.0	30.0	35.0	2.0	36.5
84-85	29.216334261037517	35.0	30.0	35.0	2.0	36.0
86-87	29.064530394683175	34.5	30.0	35.0	2.0	36.0
88-89	28.939532282907585	34.0	30.0	35.0	2.0	36.0
90-91	28.831166433047727	34.0	29.5	35.0	2.0	35.0
92-93	28.73473442188819	34.0	29.0	35.0	2.0	35.0
94-95	28.669161987776807	34.0	29.0	35.0	2.0	35.0
96-97	28.572899265702084	34.0	29.0	35.0	2.0	35.0
98-99	28.513325655422225	34.0	29.0	35.0	2.0	35.0
100-101	28.453824709226463	34.0	29.0	35.0	2.0	35.0
102-103	27.51249561801715	32.5	26.5	34.5	2.0	35.0
104-105	28.315865825117605	34.0	29.0	35.0	2.0	35.0
106-107	28.628900615847645	34.5	30.0	35.0	2.0	35.0
108-109	28.673289776554036	35.0	30.0	35.0	2.0	35.0
110-111	28.61750016798686	35.0	30.0	35.0	2.0	35.0
112-113	28.55514474737641	35.0	30.0	35.0	2.0	35.0
114-115	28.446286917391618	35.0	29.5	35.0	2.0	35.0
116-117	28.38980061912925	35.0	29.0	35.0	2.0	35.0
118-119	28.238165520970227	35.0	29.0	35.0	2.0	35.0
120-121	28.07822848609199	34.5	29.0	35.0	2.0	35.0
122-123	27.96603826558384	34.0	29.0	35.0	2.0	35.0
124-125	27.14814383634069	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80524.0
3	13371.0
4	8749.0
5	7459.0
6	7723.0
7	8826.0
8	9967.0
9	10393.0
10	11334.0
11	12018.0
12	11934.0
13	12585.0
14	15053.0
15	12963.0
16	8803.0
17	7707.0
18	6960.0
19	6336.0
20	6032.0
21	6327.0
22	7511.0
23	10251.0
24	14744.0
25	20725.0
26	24742.0
27	25731.0
28	30001.0
29	35742.0
30	40122.0
31	47725.0
32	60320.0
33	78545.0
34	109735.0
35	172247.0
36	328894.0
37	499657.0
38	147852.0
39	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57189255549366	17.264718280979608	12.54868836476398	24.614700798762748
2	16.576277385763806	19.352041978001253	38.77661073802774	25.295069898207203
3	18.791750486768798	24.72379094550117	28.91831297944187	27.566145588288165
4	13.37111272751525	15.577278591421894	35.8326229723449	35.21898570871795
5	14.429184343615924	37.09095513363617	33.39773630837562	15.082124214372284
6	33.15753844774853	35.00864817033482	17.412396561994015	14.421416819922634
7	29.47922539871789	31.379408095565147	20.913056936174915	18.22830956954205
8	27.757102749390867	33.52778049893806	19.448694086235538	19.266422665435545
9	27.771997075896937	14.87520196996594	19.054084964680982	38.29871598945614
10-11	26.169718627644084	25.038898563593026	27.000188630676767	21.79119417808612
12-13	26.62382055071189	22.85031233130254	27.07006767699295	23.45579944099262
14-15	24.157321081269792	23.90666095227922	24.45482529215674	27.481192674294245
16-17	24.142941445437526	26.82400542960725	24.909339367388224	24.123713757566996
18-19	24.223650229527234	25.995271611349697	26.48773175188806	23.293346407235013
20-21	25.43741738974528	24.921116327747455	26.159097447786152	23.482368834721115
22-23	25.223846954636482	24.59573869299593	26.012317738768566	24.168096613599026
24-25	24.609455931711764	25.02656270400899	26.090843014354153	24.273138349925087
26-27	24.28602101561397	25.080477512938675	26.2082570944504	24.425244376996954
28-29	24.345759422492243	25.03027829978748	26.297849875916295	24.326112401803982
30-31	24.709209686661207	25.00607781003932	26.051539307992144	24.23317319530733
32-33	24.385386717562067	25.082210332846632	26.004081395460954	24.528321554130347
34-35	24.592927444096325	25.111859460797405	26.132292881196957	24.162920213909317
36-37	24.716633825079853	24.854844702984717	25.99755994219325	24.430961529742177
38-39	24.64713873628486	25.14228203205766	25.949860747463106	24.260718484194374
40-41	24.678435959618806	24.96235401840725	26.14779529653881	24.211414725435134
42-43	24.508279449094736	25.026339496632215	25.99754572961383	24.467835324659216
44-45	24.445759581630018	25.14850669820911	25.87744363390646	24.528290086254408
46-47	24.63891131585819	25.15755199306985	25.745462903939544	24.458073787132413
48-49	24.720865381157832	25.31149729763815	25.512161540317592	24.455475780886424
50-51	24.695867931401626	25.486196992840917	25.353407118225203	24.464527957532255
52-53	24.79398135238714	25.350730753133604	25.465577907650065	24.389709986829192
54-55	24.679114036852248	25.25991372167266	25.59374977200551	24.467222469469583
56-57	24.603246567133066	25.37898329815785	25.40253784621798	24.6152322884911
58-59	24.678547494301558	25.184814997399613	25.505433712048177	24.63120379625065
60-61	24.702056839509826	25.377769834479498	25.552429763469185	24.367743562541488
62-63	24.571658902965286	25.46648650085228	25.56891107899903	24.39294351718341
64-65	24.644787471756004	25.123007287159094	25.635646214262486	24.596559026822415
66-67	24.574645054471226	25.35645662819008	25.532629977346538	24.536268339992155
68-69	24.59636397362353	25.402945638323676	25.483812223828494	24.5168781642243
70-71	24.56196167791679	25.16731874399874	25.609662991321702	24.661056586762765
72-73	24.76030934885375	25.445180720537415	25.34831706564089	24.44619286496795
74-75	24.51967344412027	25.622687994480632	25.346589135612817	24.511049425786283
76-77	24.588451715584554	25.273012952630165	25.489406834305488	24.64912849747979
78-79	24.559667565490383	25.34985084736931	25.443875623640366	24.64660596349994
80-81	24.565419002563406	25.574083529583397	25.276947043744656	24.583550424108537
82-83	24.7319483029666	25.424359289953774	25.32920194690934	24.514490460170283
84-85	24.559935147514917	25.546450436144426	25.198274698830332	24.69533971751033
86-87	24.34726501488438	25.595583554141733	25.42834919368031	24.628802237293577
88-89	24.533913526882277	25.37164090416621	25.37393333557366	24.720512233377853
90-91	24.48663936522854	25.42823682755969	25.430867642898857	24.654256164312912
92-93	24.578349408493498	25.57302436516012	25.27801352595392	24.570612700392463
94-95	24.557061668310315	25.24463261736974	25.423115574747946	24.775190139572008
96-97	24.60081173879952	25.325057577609307	25.390306123777922	24.68382455981325
98-99	24.39436128121443	25.389550345517392	25.326099130801712	24.88998924246647
100-101	24.397661050245638	25.43244497036981	25.439008907940515	24.730885071444035
102-103	24.465514021539622	25.28919661515004	25.521996862484027	24.723292500826314
104-105	24.366289005188147	25.50354502049707	25.368078640824997	24.762087333489784
106-107	24.345651061825844	25.481554142542944	25.402658237211778	24.770136558419438
108-109	24.378778402897144	25.42514643447964	25.386647717726785	24.809427444896432
110-111	24.359590784045807	25.575398076024484	25.35848590722056	24.706525232709147
112-113	24.360832693234855	25.460447644372486	25.4963139964988	24.68240566589386
114-115	24.24532253124043	25.69930215645948	25.43802757617622	24.617347736123875
116-117	24.195438853665436	25.73923670420999	25.36468906555313	24.700635376571444
118-119	24.396651044858025	25.34079970676148	25.381814460324424	24.880734788056067
120-121	24.106926287439673	25.492042631671392	25.55809265718181	24.84293842370712
122-123	24.173068691694333	25.602924476468537	25.43723471075543	24.786772121081704
124-125	24.17619253129152	25.557324612660278	25.504947441814068	24.76153541423414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	79.5
2	101.0
3	120.0
4	113.5
5	109.5
6	127.0
7	161.5
8	250.5
9	311.0
10	364.5
11	470.5
12	561.5
13	629.5
14	709.5
15	834.0
16	951.0
17	1037.0
18	1186.0
19	1323.5
20	1458.5
21	1617.0
22	1885.5
23	2314.0
24	2739.5
25	3460.5
26	4646.5
27	6011.5
28	7001.5
29	8531.5
30	11046.0
31	13490.5
32	16530.0
33	20065.5
34	23798.0
35	27414.0
36	31601.5
37	35729.0
38	38069.0
39	40949.0
40	43677.0
41	45726.5
42	48167.0
43	52638.5
44	57304.5
45	60648.0
46	64311.5
47	67788.0
48	72442.0
49	76498.5
50	77016.0
51	76961.5
52	77473.0
53	77971.5
54	78628.5
55	77771.0
56	74221.0
57	68551.0
58	64249.5
59	59714.5
60	53028.5
61	46720.0
62	40118.0
63	31655.5
64	24166.0
65	19139.0
66	15041.0
67	11849.0
68	9603.0
69	7783.5
70	6472.5
71	5290.0
72	4284.0
73	3804.0
74	3168.5
75	2537.0
76	1910.0
77	1241.5
78	792.5
79	513.5
80	344.0
81	207.0
82	120.5
83	80.0
84	57.5
85	44.0
86	32.0
87	24.0
88	27.5
89	18.5
90	15.0
91	13.5
92	8.0
93	4.5
94	3.5
95	8.0
96	10.5
97	8.0
98	5.0
99	6.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036462264421216244
2	0.026148652484929365
3	0.026252830383275697
4	0.030263679469609485
5	0.03265977113157512
6	0.0463591647641178
7	0.039952224015818376
8	0.03526421859023343
9	0.02979487892705099
10-11	0.03641017547204308
12-13	0.03526421859023343
14-15	0.03440475092887619
16-17	0.03570697465820534
18-19	0.04987516883330651
20-21	0.059641846803275146
22-23	0.05289632788535014
24-25	0.06352247351667602
26-27	0.048651078527737104
28-29	0.04828645588352494
30-31	0.04831250035811153
32-33	0.05659464327664493
34-35	0.03935320110032696
36-37	0.051359703884741745
38-39	0.060240869718766556
40-41	0.04841667825645786
42-43	0.057115532768376595
44-45	0.05774060015845458
46-47	0.0635485179912626
48-49	0.04771347744262012
50-51	0.05063045859631742
52-53	0.060214825244179974
54-55	0.046202897916598304
56-57	0.043910984152979
58-59	0.044119339949671654
60-61	0.03307648272496045
62-63	0.04271293832199617
64-65	0.041723248287706016
66-67	0.03440475092887619
68-69	0.030341812893369235
70-71	0.048208322459765196
72-73	0.03127941397848622
74-75	0.03823328869310389
76-77	0.03164403662269838
78-79	0.03219097058901663
80-81	0.02463807295890755
82-83	0.017736287193463044
84-85	0.018752021702339784
86-87	0.01724144217631797
88-89	0.02258055946656749
90-91	0.01229299200486719
92-93	0.019377089092417775
94-95	0.01468908366683283
96-97	0.010938679326364874
98-99	0.01083450142801854
100-101	0.011120990648470955
102-103	0.006328807324539678
104-105	0.006693429968751839
106-107	0.009115566105304061
108-109	0.012944103869531768
110-111	0.006354851799126261
112-113	0.008672810037332149
114-115	0.0085946766135724
116-117	0.007500808680935914
118-119	0.24109370124799911
120-121	0.3567572128870144
122-123	0.02831034387561576
124-125	0.0033076482724960455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1919793.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.17755882546841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27617019541354	46.70271613826986
2	10.25049274242294	11.926972890246955
3	3.5103314144240674	6.126675370786376
4	1.6945645482912128	3.9434251476706144
5	1.0188295480013614	2.9636507980987297
6	0.6559087147618605	2.2895500702317313
7	0.4508505375944586	1.8360568570676972
8	0.3143858847794309	1.463216264452182
9	0.2701084357509406	1.4142824469140047
>10	1.4463362900908576	15.044770042817538
>50	0.08440212599768106	3.322524577048226
>100	0.027168436894008743	2.650847343110013
>500	2.7067534652265E-4	0.12519048561465768
>1k	1.8045023101509927E-4	0.19012156767139995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.1058968336690466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.208894917316606E-5
7	0.0	0.0	0.0	0.0	5.208894917316606E-5
8	0.0	0.0	0.0	0.0	5.208894917316606E-5
9	0.0	0.0	0.0	0.0	5.208894917316606E-5
10-11	5.208894917316606E-5	0.0	0.0	5.208894917316606E-5	5.208894917316606E-5
12-13	5.208894917316606E-5	0.0	0.0	5.208894917316606E-5	2.0835579669266428E-4
14-15	5.208894917316606E-5	0.0	0.0	5.208894917316606E-5	3.125336950389964E-4
16-17	5.208894917316606E-5	0.0	0.0	5.208894917316606E-5	3.6462264421216245E-4
18-19	5.208894917316606E-5	0.0	0.0	5.208894917316606E-5	5.208894917316607E-4
20-21	7.81334237597491E-5	0.0	0.0	5.208894917316606E-5	5.469339663182436E-4
22-23	1.0417789834633213E-4	0.0	0.0	7.81334237597491E-5	6.250673900779928E-4
24-25	1.0417789834633213E-4	0.0	0.0	1.562668475194982E-4	6.771563392511589E-4
26-27	1.0417789834633213E-4	0.0	0.0	2.0835579669266428E-4	7.292452884243249E-4
28-29	1.0417789834633213E-4	0.0	0.0	6.250673900779928E-4	7.292452884243249E-4
30-31	1.0417789834633213E-4	0.0	0.0	0.002213780339859558	7.292452884243249E-4
32-33	1.0417789834633213E-4	0.0	0.0	0.005625606510701935	7.292452884243249E-4
34-35	1.0417789834633213E-4	0.0	0.0	0.011251213021403871	7.81334237597491E-4
36-37	1.0417789834633213E-4	0.0	0.0	0.0190385109227922	7.81334237597491E-4
38-39	1.0417789834633213E-4	0.0	0.0	0.03180030347021788	7.81334237597491E-4
40-41	1.0417789834633213E-4	0.0	0.0	0.046254986865771466	7.81334237597491E-4
42-43	1.0417789834633213E-4	0.0	0.0	0.06102220395636404	7.81334237597491E-4
44-45	1.0417789834633213E-4	0.0	0.0	0.07792506796305643	8.594676613572401E-4
46-47	1.0417789834633213E-4	0.0	0.0	0.09753655732675345	9.376010851169891E-4
48-49	1.0417789834633213E-4	0.0	0.0	0.11816378119932722	9.376010851169891E-4
50-51	1.0417789834633213E-4	0.0	0.0	0.14162985280183854	9.896900342901552E-4
52-53	1.0417789834633213E-4	0.0	0.0	0.16348116697998172	0.0011199124072230704
54-55	1.0417789834633213E-4	0.0	0.0	0.18884848522731357	0.0012240903055694024
56-57	1.0417789834633213E-4	0.0	0.0	0.2170286067299964	0.0012501347801559856
58-59	1.0417789834633213E-4	0.0	0.0	0.2510687350146604	0.0012761792547425687
60-61	1.0417789834633213E-4	0.0	0.0	0.2875309994358767	0.0013543126785023178
62-63	1.0417789834633213E-4	0.0	0.0	0.32732695660417555	0.0013543126785023178
64-65	1.0417789834633213E-4	0.0	0.0	0.36618531268735743	0.0013803571530889007
66-67	1.0417789834633213E-4	0.0	0.0	0.40947122945025843	0.0014064016276754837
68-69	1.0417789834633213E-4	0.0	0.0	0.4613257783521453	0.0014064016276754837
70-71	1.0417789834633213E-4	0.0	0.0	0.5189361561376669	0.0014324461022620668
72-73	1.562668475194982E-4	0.0	0.0	0.5830055636206612	0.001640801898954731
74-75	1.562668475194982E-4	0.0	0.0	0.645225813408008	0.001744979797301063
76-77	1.562668475194982E-4	0.0	0.0	0.7164053624531395	0.0017710242718876462
78-79	1.562668475194982E-4	0.0	0.0	0.7970651002477871	0.0017710242718876462
80-81	1.562668475194982E-4	0.0	0.0	0.8827774661122318	0.0018231132210608124
82-83	1.562668475194982E-4	0.0	0.0	0.9776835315057404	0.0019793800685803105
84-85	1.562668475194982E-4	0.0	0.0	1.0797518274105593	0.0019793800685803105
86-87	1.562668475194982E-4	0.0	0.0	1.188279153012851	0.0021356469160998086
88-89	1.562668475194982E-4	0.0	0.0	1.3126154746891983	0.002239824814446141
90-91	1.562668475194982E-4	0.0	0.0	1.4494010552179324	0.002239824814446141
92-93	1.562668475194982E-4	0.0	0.0	1.6008496749389127	0.002239824814446141
94-95	1.562668475194982E-4	0.0	0.0	1.7583145682893937	0.002344002712792473
96-97	1.562668475194982E-4	0.0	0.0	1.9244783161517933	0.002500269560311971
98-99	1.562668475194982E-4	0.0	0.0	2.1087690183264547	0.002552358509485137
100-101	1.562668475194982E-4	0.0	0.0	2.313947389119556	0.002552358509485137
102-103	1.562668475194982E-4	0.0	0.0	2.5373568921232654	0.0026304919332448863
104-105	1.562668475194982E-4	0.0	0.0	2.7746220556070367	0.0026565364078314693
106-107	1.562668475194982E-4	0.0	0.0	3.02170598600995	0.002682580882418052
108-109	1.562668475194982E-4	0.0	0.0	3.2772804151280894	0.0027607143061778015
110-111	1.562668475194982E-4	0.0	0.0	3.5670251949038256	0.0029169811536972996
112-113	1.562668475194982E-4	0.0	0.0	3.884924051707658	0.003021159052043632
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2230	0.0	70.45423	1
GTATCAA	4525	0.0	60.893433	1
TCAACGC	5930	0.0	45.44928	4
CAACGCA	5980	0.0	44.969776	5
AACGCAG	6350	0.0	42.442078	6
ATCAACG	6450	0.0	42.058586	3
TATCAAC	6730	0.0	41.103245	2
ACGCAGA	7380	0.0	36.35736	7
GTACATG	9790	0.0	35.379223	1
CGCAGAG	7785	0.0	34.386826	8
TACATGG	10085	0.0	33.623055	2
ACATGGG	10285	0.0	32.79656	3
GCAGAGT	8415	0.0	30.54071	9
CATGGGG	8645	0.0	30.212244	4
AGAGTAC	7570	0.0	27.781054	10-11
ATGGGGG	4680	0.0	27.459421	5
CAGAGTA	7955	0.0	25.800856	10-11
GAGTACT	3255	0.0	23.029284	12-13
GTGGTAT	1205	0.0	21.730698	1
GTACTTT	3490	0.0	21.653297	14-15
>>END_MODULE