FastQCFastQC Report
Mon 15 Aug 2016
SRR2921547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919793
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30960.16126738664012213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.1460053245323845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.078.5743941
GTATCAA59350.062.559851
TCAACGC80100.046.1554574
CAACGCA81500.045.191775
ATCAACG84150.043.792583
TATCAAC85650.042.9561232
AACGCAG86450.042.807296
ACGCAGA97150.037.7211577
CGCAGAG101000.036.222488
GTACATG93150.033.141741
GCAGAGT109950.032.623859
TACATGG97050.031.6532362
ACATGGG96100.031.5324953
AGAGTAC102950.028.51503810-11
CATGGGG79200.028.3387174
CAGAGTA108650.026.77270710-11
GAGTACT62900.024.77850712-13
AGTACTT67750.024.40956112-13
GTACTTT66200.023.63564714-15
ATGGGGG46250.022.77135
Produced by FastQC (version 0.11.5)