FastQCFastQC Report
Mon 15 Aug 2016
SRR2921546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045143
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23650.11563983545404893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.074.397241
GTATCAA45150.057.0790821
TCAACGC57300.044.2325944
CAACGCA59200.042.8119165
ATCAACG62200.040.748033
AACGCAG63650.039.9093256
GTACATG116550.039.4231951
TACATGG119950.037.7460752
ACATGGG122400.036.553073
TATCAAC70800.035.9664762
CATGGGG100500.033.980774
ACGCAGA74450.033.8785487
CGCAGAG79450.031.5944188
ATGGGGG53050.030.0556565
GTATACG1203.6898717E-529.7588961
GCAGAGT85650.028.6128869
GTATAGG5550.027.8822081
ATGGGGA39950.026.2103425
AGAGTAC76650.025.60905610-11
ATGGGTA17050.025.472725
Produced by FastQC (version 0.11.5)