FastQCFastQC Report
Mon 15 Aug 2016
SRR2921546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045143
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.13827883918141667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.12478345035041559No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22280.10894103737489262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.084.644981
GTATCAA61850.062.456721
TCAACGC84150.044.8587574
CAACGCA86000.044.0074735
ATCAACG88650.042.9195823
AACGCAG90650.041.741896
TATCAAC94050.040.708542
GTACATG109600.038.2374231
TACATGG113950.036.2642362
ACGCAGA103400.036.247797
ACATGGG118450.034.6351853
CGCAGAG109450.034.1881188
GCAGAGT115300.032.195539
CATGGGG96700.031.6481844
AGAGTAC107950.027.88586210-11
CAGAGTA111450.027.83751710-11
GAGTACT57800.025.78347412-13
ATGGGGG51250.025.544455
CATGGGT21600.025.3598214
AGTACTT60700.024.11059612-13
Produced by FastQC (version 0.11.5)