FastQCFastQC Report
Mon 15 Aug 2016
SRR2921545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563225
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.071.0354161
GTATCAA34450.057.7107851
TCAACGC43700.044.9329954
CAACGCA45150.043.4899645
ATCAACG47150.041.76743
AACGCAG47500.041.211776
TATCAAC49000.040.4333082
GTACATG80900.034.8762321
ACGCAGA56150.034.7559477
TACATGG83700.033.4800872
CGCAGAG59600.032.640068
ACATGGG85400.032.3259473
CATGGGG72900.029.4654124
GCAGAGT66500.028.7184269
AGAGTAC59150.025.4469710-11
CAGAGTA63300.024.95347810-11
ATGGGGG39550.022.5671965
ATGGGGA30150.022.3009825
GTATAGG4950.021.6454721
ACACCGT6400.020.4532186
Produced by FastQC (version 0.11.5)