##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921545_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1563225
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.681709286890882	31.0	30.0	34.0	25.0	34.0
2	29.800067808536838	31.0	30.0	34.0	25.0	34.0
3	29.977988453357643	31.0	30.0	34.0	25.0	34.0
4	33.33777927041852	37.0	35.0	37.0	27.0	37.0
5	33.39723968078811	37.0	35.0	37.0	27.0	37.0
6	33.37971757104703	37.0	35.0	37.0	27.0	37.0
7	33.19779238433367	37.0	35.0	37.0	26.0	37.0
8	33.20215835852164	37.0	35.0	37.0	26.0	37.0
9	34.64702330118825	39.0	35.0	39.0	26.0	39.0
10-11	34.53357386172816	39.0	35.0	39.0	25.5	39.0
12-13	34.392418557789185	39.0	35.0	39.0	25.0	39.0
14-15	35.54953445601241	40.0	36.0	41.0	25.0	41.0
16-17	35.481273329175266	40.0	36.0	41.0	25.0	41.0
18-19	35.49209102976219	40.0	36.0	41.0	25.0	41.0
20-21	35.5131791648675	40.0	36.0	41.0	25.0	41.0
22-23	35.475066289241795	40.0	36.0	41.0	25.0	41.0
24-25	35.43892977658366	40.0	35.5	41.0	25.0	41.0
26-27	35.3619299844872	40.0	35.0	41.0	24.5	41.0
28-29	35.31716483551632	40.0	35.0	41.0	24.5	41.0
30-31	35.2194485758608	40.0	35.0	41.0	24.0	41.0
32-33	35.111061747349225	40.0	35.0	41.0	24.0	41.0
34-35	34.950666410785395	40.0	35.0	41.0	24.0	41.0
36-37	34.808105678964964	39.5	35.0	41.0	23.0	41.0
38-39	34.678587535383585	39.0	34.0	41.0	22.0	41.0
40-41	34.51895056693694	39.0	34.0	41.0	21.0	41.0
42-43	34.32305362311887	39.0	34.0	41.0	19.5	41.0
44-45	34.117229445537276	39.0	33.0	41.0	18.0	41.0
46-47	33.85674199171584	38.5	33.0	41.0	15.0	41.0
48-49	33.70574069631691	38.0	33.0	40.5	14.5	41.0
50-51	33.50450095155848	38.0	33.0	40.0	13.0	41.0
52-53	33.29756624926034	38.0	33.0	40.0	10.0	41.0
54-55	33.08804586671784	37.5	33.0	40.0	8.5	41.0
56-57	32.836175214700376	37.0	32.0	40.0	8.0	41.0
58-59	32.58770362551776	36.5	32.0	40.0	7.0	41.0
60-61	32.27402261350733	36.0	32.0	40.0	4.0	41.0
62-63	31.95634377648771	35.5	31.0	39.0	2.0	41.0
64-65	31.64704313198676	35.0	31.0	39.0	2.0	41.0
66-67	31.602527147403606	35.0	31.0	39.0	2.0	41.0
68-69	31.352091669465366	35.0	31.0	38.0	2.0	40.0
70-71	31.06006141150506	35.0	31.0	37.0	2.0	40.0
72-73	30.742004190055816	35.0	31.0	37.0	2.0	39.0
74-75	30.444346783092644	35.0	31.0	36.0	2.0	39.0
76-77	30.170225015592763	35.0	31.0	36.0	2.0	38.5
78-79	29.912205536631003	35.0	30.0	35.0	2.0	37.0
80-81	29.681829551088295	35.0	30.0	35.0	2.0	37.0
82-83	29.49517663803995	35.0	30.0	35.0	2.0	36.0
84-85	29.303670296982197	35.0	30.0	35.0	2.0	36.0
86-87	29.15634057797182	34.5	30.0	35.0	2.0	36.0
88-89	29.039823441922948	34.0	30.0	35.0	2.0	35.0
90-91	28.929975531353453	34.0	30.0	35.0	2.0	35.0
92-93	28.834029650242286	34.0	29.5	35.0	2.0	35.0
94-95	28.770993299109215	34.0	29.0	35.0	2.0	35.0
96-97	28.673382270626426	34.0	29.0	35.0	2.0	35.0
98-99	28.6129207247837	34.0	29.0	35.0	2.0	35.0
100-101	28.55118233139823	34.0	29.0	35.0	2.0	35.0
102-103	27.57501095491692	32.5	27.5	34.5	2.0	35.0
104-105	28.4013641670265	34.0	29.0	35.0	2.0	35.0
106-107	28.730075005197588	34.5	30.0	35.0	2.0	35.0
108-109	28.773518847254874	35.0	30.0	35.0	2.0	35.0
110-111	28.71189592029298	35.0	30.0	35.0	2.0	35.0
112-113	28.64453709478802	35.0	30.0	35.0	2.0	35.0
114-115	28.528328935373985	35.0	30.0	35.0	2.0	35.0
116-117	28.468183402901055	35.0	30.0	35.0	2.0	35.0
118-119	28.31306785651458	35.0	29.5	35.0	2.0	35.0
120-121	28.145398455116826	34.5	29.0	35.0	2.0	35.0
122-123	28.01988741224072	34.0	29.0	35.0	2.0	35.0
124-125	27.167792544259463	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62474.0
3	10974.0
4	7107.0
5	6289.0
6	6509.0
7	7431.0
8	8215.0
9	8516.0
10	9010.0
11	9309.0
12	9422.0
13	9819.0
14	10823.0
15	9268.0
16	6823.0
17	6026.0
18	5560.0
19	5196.0
20	5146.0
21	5253.0
22	6162.0
23	8374.0
24	12237.0
25	16466.0
26	19601.0
27	20689.0
28	24582.0
29	29265.0
30	32793.0
31	39383.0
32	50011.0
33	64833.0
34	92141.0
35	147575.0
36	283568.0
37	401207.0
38	105057.0
39	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.760620686036226	16.99725723734816	12.380508083022644	24.861613993592968
2	16.869007131453582	19.0463240540186	38.22663881059256	25.858030003935255
3	19.594881242077527	23.60530867333717	28.39427412703217	28.405535957553134
4	13.814044028529853	14.911813677998662	35.00570161910625	36.26844067436524
5	15.055229085035704	36.5310592811164	32.883463142960814	15.53024849088709
6	34.021203810669746	33.99163495908682	17.013161979064996	14.973999251178432
7	30.22893112994907	30.89706290806276	20.167596120739862	18.706409841248306
8	28.081477760321512	33.17703750395965	19.2048047048735	19.536680030845336
9	27.874307383283004	14.842458692559765	18.631141724146094	38.65209220001113
10-11	26.9129890404761	24.560199947075844	26.278411136215063	22.24839987623299
12-13	27.074948692235967	22.18264860053154	26.49326871573483	24.24913399149766
14-15	24.501508186314368	23.28974359712755	23.950357040351943	28.258391176206143
16-17	24.64950411411695	26.358154558966344	24.389138027303723	24.603203299612986
18-19	24.795785427248088	25.441495505119242	25.902328390153922	23.860390677478748
20-21	25.961981922642018	24.465627831488376	25.533107950459293	24.039282295410313
22-23	25.731483564682428	24.000245788078832	25.501889335837724	24.766381311401016
24-25	25.16745388476689	24.49194105674468	25.488102428293374	24.852502630195055
26-27	24.76930811475407	24.51281490022366	25.653961692106375	25.06391529291589
28-29	24.858184285225114	24.58789758162844	25.639954813541856	24.913963319604587
30-31	25.103877850400536	24.54112615622512	25.50828462659017	24.846711366784177
32-33	24.829737988527157	24.561479308044945	25.464700076425878	25.144082627002017
34-35	25.091947586515843	24.5976993104331	25.5904516583204	24.719901444730652
36-37	25.191984710349193	24.40103586616179	25.448038997314328	24.958940426174696
38-39	25.17063041316484	24.62486913667492	25.34707786315833	24.857422587001906
40-41	25.21817658743048	24.53494969562002	25.45793002412575	24.78894369282375
42-43	25.120497428748095	24.56569718244047	25.35301105951965	24.960794329291787
44-45	24.991982742894088	24.56161126064567	25.361800730354524	25.084605266105726
46-47	25.06904713513202	24.619924687491498	25.298601453438597	25.012426723937885
48-49	25.257005487647994	24.772724072559328	24.946845210436646	25.02342522935604
50-51	25.129897643601062	25.058340331186184	24.866166064168603	24.94559596104415
52-53	25.249490233706762	24.84689408223024	24.969858897714207	24.933756786348795
54-55	25.154884361543623	24.75314504856457	25.11737930540124	24.97459128449057
56-57	25.071249048785997	24.814221265337025	24.906702626662664	25.20782705921431
58-59	25.18324521882383	24.739200572432303	24.985639591015552	25.09191461772831
60-61	25.15632854223374	24.826761129859477	25.133514798056765	24.88339552985001
62-63	25.026055741527042	24.931688677648317	25.01066389421417	25.031591686610472
64-65	25.086006431242698	24.685469737031994	25.07487063435963	25.153653197365678
66-67	25.04320464040716	24.85864299192095	25.058819426201417	25.03933294147047
68-69	25.031227763017117	24.874513026072623	24.99552060776394	25.098738603146327
70-71	24.989207381798828	24.68410190535714	25.127103280475904	25.199587432368126
72-73	25.184864751408863	24.917346394517427	24.916642483041333	24.981146371032377
74-75	24.96314559211042	25.066309135445348	24.915147665620847	25.055397606823387
76-77	25.04887390053721	24.822534147738363	24.93627995226196	25.19231199946247
78-79	24.981002060896433	24.876789365889586	24.970603188966063	25.17160538424792
80-81	24.99000199000391	25.02436315075847	24.871305615330325	25.114329243907292
82-83	25.14031141071511	24.882753916946907	24.934674897245728	25.042259775092262
84-85	24.953396392831216	25.0315196183031	24.81116221574359	25.20392177312209
86-87	24.77010569459671	25.053416735633625	24.970368467506727	25.206109102262936
88-89	24.90065504802886	24.89956730038631	24.92964032344496	25.270137328139867
90-91	24.84223012137916	24.877290094406014	24.935830012872383	25.344649771342436
92-93	24.919020860226407	25.025073541055825	24.90539237092525	25.15051322779252
94-95	24.891609748388742	24.88306840782054	24.9272146624427	25.298107181348016
96-97	24.914708848878956	24.87331484998514	24.94385099184052	25.268125309295385
98-99	24.836608415846175	24.881073063092778	24.831714105753566	25.45060441530748
100-101	24.810409947004032	24.98203025059763	24.957942629235845	25.249617173162488
102-103	24.852882799802575	24.798856393076814	25.09851262647345	25.24974818064716
104-105	24.68886855214121	24.95736906601549	24.934210336917925	25.419552044925375
106-107	24.76219356741477	24.971138870255906	24.99186706318932	25.274800499140003
108-109	24.76963703337604	24.889468961579247	24.92753623188406	25.413357773160655
110-111	24.786741167047527	25.053386679495677	24.98151138361384	25.178360769842957
112-113	24.768425665582285	25.046110146151413	24.948068108028572	25.237396080237733
114-115	24.578552891648084	25.240924024844276	24.972866358538965	25.207656724968675
116-117	24.50596324332624	25.24643860746233	24.94671705741891	25.300881091792522
118-119	24.72837875664564	24.941218870369855	24.938621695134593	25.391780677849912
120-121	24.425771387204836	25.061436498496793	25.098386679957173	25.414405434341198
122-123	24.488980379559727	25.107918180550026	24.996640554711604	25.40646088517864
124-125	24.474321074688664	25.21822656411431	25.013962022020653	25.293490339176376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	99.0
2	114.5
3	122.5
4	103.5
5	104.5
6	119.5
7	153.5
8	224.5
9	267.0
10	283.0
11	318.0
12	360.0
13	432.5
14	477.5
15	542.0
16	641.0
17	713.0
18	796.0
19	861.5
20	977.5
21	1089.5
22	1230.5
23	1491.0
24	1780.0
25	2224.5
26	2925.5
27	3706.5
28	4459.0
29	5598.0
30	7132.5
31	8529.0
32	10180.5
33	12105.0
34	13968.0
35	16026.0
36	18722.0
37	21764.5
38	24395.5
39	27387.0
40	30446.0
41	33377.0
42	36496.0
43	41062.0
44	45753.0
45	49106.5
46	53114.0
47	56688.0
48	60860.0
49	65475.0
50	66489.5
51	66581.0
52	66750.5
53	67038.0
54	68214.0
55	67689.5
56	64348.0
57	59343.0
58	55943.5
59	52209.0
60	46934.0
61	42069.0
62	36558.5
63	28996.5
64	22145.0
65	17706.5
66	13880.5
67	10774.5
68	8826.5
69	7258.0
70	6055.5
71	4959.0
72	4059.5
73	3639.5
74	2980.0
75	2368.5
76	1684.5
77	1017.0
78	673.0
79	434.5
80	250.5
81	125.5
82	76.5
83	52.5
84	43.5
85	33.0
86	23.5
87	17.0
88	11.5
89	9.0
90	9.0
91	6.5
92	5.5
93	5.5
94	6.5
95	8.0
96	7.5
97	7.0
98	7.5
99	7.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036271170177037855
2	0.02750723664219802
3	0.027123414735562696
4	0.032688832381774856
5	0.03531161541044955
6	0.04925714468486623
7	0.04215643941211278
8	0.03774248748580659
9	0.031473396344096335
10-11	0.03911784931791649
12-13	0.03812630939244191
14-15	0.03751859137360265
16-17	0.03950167122455181
18-19	0.05351117081674103
20-21	0.0636184810248045
22-23	0.057797182107502114
24-25	0.06899198771769899
26-27	0.051528090965791876
28-29	0.05181595739576836
30-31	0.05200786834908602
32-33	0.059300484575157124
34-35	0.0415807065521598
36-37	0.05507844360216859
38-39	0.06419421388475748
40-41	0.05031265492811336
42-43	0.06083577220169841
44-45	0.062051208239376925
46-47	0.0682883142222009
48-49	0.05162404644245071
50-51	0.05379903724671753
52-53	0.06329862943594172
54-55	0.049193174367093674
56-57	0.047849797693870044
58-59	0.049225159525979945
60-61	0.0364950662892418
62-63	0.04538694045962673
64-65	0.044555326328583535
66-67	0.038606086775736057
68-69	0.032816773017319965
70-71	0.052103823825744855
72-73	0.033808312942794544
74-75	0.042060483935453946
76-77	0.03390426841945337
78-79	0.03553551152265349
80-81	0.026483711557837165
82-83	0.017463896751907116
84-85	0.019798813350605318
86-87	0.01781573349965616
88-89	0.023189240192550656
90-91	0.012090390059012619
92-93	0.020630427481648513
94-95	0.015256920788754019
96-97	0.01257016744230677
98-99	0.01186649394680868
100-101	0.011610612675718467
102-103	0.0064290169361416296
104-105	0.006365046618369077
106-107	0.008731948375953558
108-109	0.01295398934894209
110-111	0.006461002095027908
112-113	0.007676438132706425
114-115	0.008220185833773128
116-117	0.006876809160549505
118-119	0.24555006476994676
120-121	0.3660701434534376
122-123	0.0298741383997825
124-125	0.003582337795262998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1563225.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.284439212440255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02167944323864	48.24062070073299
2	10.635526256527585	12.823134722078992
3	3.5854114037069813	6.484335474550908
4	1.7155583493620938	4.136858921100539
5	0.9857342585602015	2.9712218494896163
6	0.6252042598042887	2.261405291731821
7	0.45422498187037835	1.9167888815835619
8	0.32656259699597995	1.574931442212862
9	0.25697220767071033	1.3942282889350357
>10	1.318803100895951	13.815851881953106
>50	0.056499762952924644	2.2675826150157055
>100	0.017183064516032666	1.794067295847141
>500	4.26875932123348E-4	0.16823586928553472
>1k	2.13437966061674E-4	0.15073676548222098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.397031777255354E-5
2	0.0	0.0	0.0	0.0	6.397031777255354E-5
3	0.0	0.0	0.0	0.0	6.397031777255354E-5
4	0.0	0.0	0.0	0.0	6.397031777255354E-5
5	0.0	0.0	0.0	0.0	6.397031777255354E-5
6	0.0	0.0	0.0	0.0	1.2794063554510707E-4
7	0.0	0.0	0.0	0.0	1.2794063554510707E-4
8	0.0	0.0	0.0	0.0	1.2794063554510707E-4
9	0.0	0.0	0.0	0.0	1.2794063554510707E-4
10-11	0.0	0.0	0.0	0.0	1.2794063554510707E-4
12-13	0.0	0.0	0.0	0.0	2.878664299764909E-4
14-15	0.0	0.0	0.0	0.0	3.838219066353212E-4
16-17	0.0	0.0	0.0	0.0	5.117625421804283E-4
18-19	0.0	0.0	0.0	0.0	5.117625421804283E-4
20-21	0.0	0.0	0.0	3.198515888627677E-5	5.757328599529818E-4
22-23	0.0	0.0	0.0	1.2794063554510707E-4	7.356586543843656E-4
24-25	0.0	0.0	0.0	1.2794063554510707E-4	8.31614131043196E-4
26-27	0.0	0.0	0.0	2.878664299764909E-4	8.31614131043196E-4
28-29	0.0	0.0	0.0	9.275696077020262E-4	8.635992899294727E-4
30-31	0.0	0.0	0.0	0.004030130019670872	9.275696077020262E-4
32-33	0.0	0.0	0.0	0.009723488301428138	0.0010235250843608566
34-35	0.0	0.0	0.0	0.016984119368612965	0.00108749540213341
36-37	0.0	0.0	0.0	0.027891058548833343	0.00108749540213341
38-39	0.0	0.0	0.0	0.04452334116969726	0.0011194805610196868
40-41	0.0	0.0	0.0	0.06473796158582418	0.0011514657199059636
42-43	0.0	0.0	0.0	0.08444081945977067	0.0011514657199059636
44-45	0.0	0.0	0.0	0.10919733243774889	0.0011834508787922403
46-47	0.0	3.198515888627677E-5	0.0	0.13574501431335861	0.001215436037678517
48-49	0.0	6.397031777255354E-5	0.0	0.16485150889987044	0.001215436037678517
50-51	0.0	6.397031777255354E-5	0.0	0.1946296918229941	0.0014393321498824545
52-53	0.0	6.397031777255354E-5	0.0	0.22565529594268258	0.0016632282620863918
54-55	0.0	6.397031777255354E-5	0.0	0.2591437572966144	0.0019191095331766062
56-57	0.0	6.397031777255354E-5	0.0	0.29752594796014653	0.00207903532760799
58-59	0.0	6.397031777255354E-5	0.0	0.33958643189560045	0.002206975963153097
60-61	0.0	6.397031777255354E-5	0.0	0.3828303667098466	0.0022389611220393736
62-63	0.0	6.397031777255354E-5	0.0	0.4324393481424619	0.0023669017575844806
64-65	0.0	6.397031777255354E-5	0.0	0.4817604631451007	0.002398886916470757
66-67	0.0	6.397031777255354E-5	0.0	0.5402933039069872	0.002622783028674695
68-69	0.0	6.397031777255354E-5	0.0	0.6089334548769371	0.002750723664219802
70-71	0.0	6.397031777255354E-5	0.0	0.6839386524652562	0.002846679140878632
72-73	0.0	6.397031777255354E-5	0.0	0.762973980073246	0.0031345455708551235
74-75	0.0	6.397031777255354E-5	0.0	0.8441842984855028	0.003230501047513954
76-77	6.397031777255354E-5	6.397031777255354E-5	0.0	0.936205600601321	0.003326456524172784
78-79	6.397031777255354E-5	6.397031777255354E-5	0.0	1.0385900941962931	0.003358441683059061
80-81	6.397031777255354E-5	6.397031777255354E-5	0.0	1.1510818979993283	0.003454397159717891
82-83	6.397031777255354E-5	6.397031777255354E-5	0.0	1.2739049081226312	0.003582337795262998
84-85	6.397031777255354E-5	6.397031777255354E-5	0.0	1.399158790321291	0.003614322954149275
86-87	6.397031777255354E-5	6.397031777255354E-5	0.0	1.5342960866158104	0.003710278430808105
88-89	6.397031777255354E-5	6.397031777255354E-5	0.0	1.6829311199603385	0.003838219066353212
90-91	6.397031777255354E-5	6.397031777255354E-5	0.0	1.8559708295350956	0.003838219066353212
92-93	6.397031777255354E-5	6.397031777255354E-5	0.0	2.0451310591885363	0.004094100337443426
94-95	6.397031777255354E-5	6.397031777255354E-5	0.0	2.235858561627405	0.004286011290761087
96-97	6.397031777255354E-5	6.397031777255354E-5	0.0	2.431639719170305	0.004573877720737578
98-99	6.397031777255354E-5	6.397031777255354E-5	0.0	2.6466119720449712	0.004669833197396408
100-101	6.397031777255354E-5	6.397031777255354E-5	0.0	2.891938140702714	0.004669833197396408
102-103	6.397031777255354E-5	6.397031777255354E-5	0.0	3.1566793008044267	0.004797773832941514
104-105	6.397031777255354E-5	9.59554766588303E-5	0.0	3.445857122295255	0.004861744150714068
106-107	6.397031777255354E-5	1.2794063554510707E-4	0.0	3.744502550816421	0.004893729309600345
108-109	6.397031777255354E-5	1.2794063554510707E-4	0.0	4.050056773657023	0.004989684786259176
110-111	6.397031777255354E-5	1.2794063554510707E-4	0.0	4.399878456396232	0.005117625421804283
112-113	6.397031777255354E-5	1.2794063554510707E-4	0.0	4.766108525644102	0.00524556605734939
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1810	0.0	71.035416	1
GTATCAA	3445	0.0	57.710785	1
TCAACGC	4370	0.0	44.932995	4
CAACGCA	4515	0.0	43.489964	5
ATCAACG	4715	0.0	41.7674	3
AACGCAG	4750	0.0	41.21177	6
TATCAAC	4900	0.0	40.433308	2
GTACATG	8090	0.0	34.876232	1
ACGCAGA	5615	0.0	34.755947	7
TACATGG	8370	0.0	33.480087	2
CGCAGAG	5960	0.0	32.64006	8
ACATGGG	8540	0.0	32.325947	3
CATGGGG	7290	0.0	29.465412	4
GCAGAGT	6650	0.0	28.718426	9
AGAGTAC	5915	0.0	25.44697	10-11
CAGAGTA	6330	0.0	24.953478	10-11
ATGGGGG	3955	0.0	22.567196	5
ATGGGGA	3015	0.0	22.300982	5
GTATAGG	495	0.0	21.645472	1
ACACCGT	640	0.0	20.453218	6
>>END_MODULE